Experiment: LB Anaerobic with Spiramycin 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mhpC and mhpD are separated by 9 nucleotides mhpD and mhpF overlap by 4 nucleotides mhpF and mhpE overlap by 4 nucleotides
b0349: mhpC - 2-hydroxy-6-ketonona-2,4-dienedioic acid hydrolase (VIMSS), at 370,448 to 371,329
mhpC
b0350: mhpD - 2-keto-4-pentenoate hydratase (VIMSS), at 371,339 to 372,148
mhpD
b0351: mhpF - acetaldehyde dehydrogenase (NCBI), at 372,145 to 373,095
mhpF
b0352: mhpE - 4-hydroxy-2-ketovalerate aldolase (NCBI), at 373,092 to 374,105
mhpE
Position (kb) 372
373
374 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 371.194 kb on + strand, within mhpC at 371.196 kb on + strand, within mhpC at 371.237 kb on + strand, within mhpC at 371.247 kb on - strand at 371.280 kb on - strand at 371.325 kb on + strand at 371.331 kb on - strand at 371.331 kb on - strand at 371.331 kb on - strand at 371.360 kb on - strand at 371.472 kb on - strand, within mhpD at 371.489 kb on + strand, within mhpD at 371.511 kb on + strand, within mhpD at 371.522 kb on + strand, within mhpD at 371.522 kb on + strand, within mhpD at 371.530 kb on - strand, within mhpD at 371.753 kb on - strand, within mhpD at 371.791 kb on + strand, within mhpD at 371.815 kb on + strand, within mhpD at 371.936 kb on + strand, within mhpD at 371.943 kb on - strand, within mhpD at 371.982 kb on + strand, within mhpD at 371.996 kb on - strand, within mhpD at 371.996 kb on - strand, within mhpD at 371.999 kb on + strand, within mhpD at 371.999 kb on + strand, within mhpD at 372.009 kb on - strand, within mhpD at 372.028 kb on - strand, within mhpD at 372.181 kb on - strand at 372.181 kb on - strand at 372.191 kb on - strand at 372.191 kb on - strand at 372.193 kb on + strand at 372.193 kb on + strand at 372.226 kb on + strand at 372.226 kb on + strand at 372.271 kb on + strand, within mhpF at 372.307 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.308 kb on + strand, within mhpF at 372.619 kb on - strand, within mhpF at 372.648 kb on + strand, within mhpF at 372.779 kb on - strand, within mhpF at 372.779 kb on - strand, within mhpF at 372.793 kb on + strand, within mhpF at 372.814 kb on + strand, within mhpF at 372.820 kb on + strand, within mhpF at 372.844 kb on - strand, within mhpF at 372.854 kb on - strand, within mhpF at 372.903 kb on + strand, within mhpF at 372.991 kb on + strand, within mhpF at 372.991 kb on + strand, within mhpF at 373.062 kb on + strand at 373.130 kb on + strand at 373.130 kb on + strand at 373.393 kb on + strand, within mhpE at 373.429 kb on + strand, within mhpE at 373.429 kb on + strand, within mhpE at 373.437 kb on - strand, within mhpE at 373.552 kb on - strand, within mhpE at 373.576 kb on + strand, within mhpE at 373.576 kb on + strand, within mhpE at 373.640 kb on + strand, within mhpE at 373.640 kb on + strand, within mhpE at 373.736 kb on - strand, within mhpE at 373.787 kb on - strand, within mhpE at 373.787 kb on - strand, within mhpE at 373.857 kb on - strand, within mhpE at 373.857 kb on - strand, within mhpE at 373.912 kb on - strand, within mhpE
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Spiramycin 0.000125 mM remove 371,194 + mhpC b0349 0.85 +0.7 371,196 + mhpC b0349 0.85 +0.1 371,237 + mhpC b0349 0.89 +0.0 371,247 - -0.3 371,280 - +0.2 371,325 + -0.4 371,331 - +1.8 371,331 - -0.6 371,331 - -0.1 371,360 - -1.8 371,472 - mhpD b0350 0.16 +0.3 371,489 + mhpD b0350 0.19 +0.3 371,511 + mhpD b0350 0.21 -0.1 371,522 + mhpD b0350 0.23 +0.6 371,522 + mhpD b0350 0.23 -1.8 371,530 - mhpD b0350 0.24 +0.1 371,753 - mhpD b0350 0.51 -0.3 371,791 + mhpD b0350 0.56 +0.1 371,815 + mhpD b0350 0.59 +0.6 371,936 + mhpD b0350 0.74 +0.8 371,943 - mhpD b0350 0.75 -0.8 371,982 + mhpD b0350 0.79 +0.5 371,996 - mhpD b0350 0.81 +0.8 371,996 - mhpD b0350 0.81 -1.0 371,999 + mhpD b0350 0.81 -1.2 371,999 + mhpD b0350 0.81 -0.7 372,009 - mhpD b0350 0.83 +0.4 372,028 - mhpD b0350 0.85 +0.2 372,181 - +0.5 372,181 - -1.0 372,191 - +3.1 372,191 - -1.2 372,193 + +0.5 372,193 + +0.3 372,226 + +0.0 372,226 + -0.8 372,271 + mhpF b0351 0.13 -0.2 372,307 + mhpF b0351 0.17 -0.6 372,308 + mhpF b0351 0.17 -1.4 372,308 + mhpF b0351 0.17 -0.2 372,308 + mhpF b0351 0.17 +0.8 372,619 - mhpF b0351 0.50 -1.5 372,648 + mhpF b0351 0.53 +1.2 372,779 - mhpF b0351 0.67 -2.0 372,779 - mhpF b0351 0.67 +0.8 372,793 + mhpF b0351 0.68 -0.1 372,814 + mhpF b0351 0.70 +0.1 372,820 + mhpF b0351 0.71 +0.7 372,844 - mhpF b0351 0.74 +0.2 372,854 - mhpF b0351 0.75 -0.2 372,903 + mhpF b0351 0.80 +0.2 372,991 + mhpF b0351 0.89 +0.8 372,991 + mhpF b0351 0.89 +0.2 373,062 + -0.1 373,130 + +0.5 373,130 + +0.2 373,393 + mhpE b0352 0.30 -0.5 373,429 + mhpE b0352 0.33 +0.3 373,429 + mhpE b0352 0.33 +0.6 373,437 - mhpE b0352 0.34 +0.8 373,552 - mhpE b0352 0.45 -0.5 373,576 + mhpE b0352 0.48 +1.1 373,576 + mhpE b0352 0.48 +0.5 373,640 + mhpE b0352 0.54 -0.4 373,640 + mhpE b0352 0.54 -0.6 373,736 - mhpE b0352 0.64 -1.0 373,787 - mhpE b0352 0.69 +0.4 373,787 - mhpE b0352 0.69 +0.4 373,857 - mhpE b0352 0.75 +0.7 373,857 - mhpE b0352 0.75 -0.2 373,912 - mhpE b0352 0.81 +0.1
Or see this region's nucleotide sequence