Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt deoB and deoD are separated by 56 nucleotides deoD and yjjJ are separated by 166 nucleotides yjjJ and lplA are separated by 0 nucleotides
b4383: deoB - phosphopentomutase (NCBI), at 4,617,626 to 4,618,849
deoB
b4384: deoD - purine nucleoside phosphorylase (NCBI), at 4,618,906 to 4,619,625
deoD
b4385: yjjJ - predicted DNA-binding transcriptional regulator (NCBI), at 4,619,792 to 4,621,123
yjjJ
b4386: lplA - lipoate-protein ligase A (NCBI), at 4,621,124 to 4,622,140
lplA
Position (kb)
4619
4620
4621
4622 Strain fitness (log2 ratio)
-2
-1
0
1 at 4618.851 kb on - strand at 4618.864 kb on + strand at 4618.872 kb on - strand at 4618.977 kb on - strand at 4618.991 kb on - strand, within deoD at 4619.050 kb on - strand, within deoD at 4619.050 kb on - strand, within deoD at 4619.089 kb on + strand, within deoD at 4619.094 kb on + strand, within deoD at 4619.094 kb on + strand, within deoD at 4619.104 kb on + strand, within deoD at 4619.104 kb on + strand, within deoD at 4619.109 kb on + strand, within deoD at 4619.117 kb on - strand, within deoD at 4619.119 kb on + strand, within deoD at 4619.119 kb on + strand, within deoD at 4619.128 kb on + strand, within deoD at 4619.131 kb on + strand, within deoD at 4619.198 kb on + strand, within deoD at 4619.223 kb on + strand, within deoD at 4619.272 kb on + strand, within deoD at 4619.279 kb on + strand, within deoD at 4619.298 kb on + strand, within deoD at 4619.298 kb on + strand, within deoD at 4619.316 kb on - strand, within deoD at 4619.335 kb on + strand, within deoD at 4619.335 kb on + strand, within deoD at 4619.336 kb on - strand, within deoD at 4619.340 kb on + strand, within deoD at 4619.355 kb on + strand, within deoD at 4619.375 kb on - strand, within deoD at 4619.375 kb on - strand, within deoD at 4619.382 kb on + strand, within deoD at 4619.382 kb on + strand, within deoD at 4619.385 kb on - strand, within deoD at 4619.385 kb on - strand, within deoD at 4619.390 kb on - strand, within deoD at 4619.390 kb on - strand, within deoD at 4619.512 kb on + strand, within deoD at 4619.513 kb on + strand, within deoD at 4619.513 kb on + strand, within deoD at 4619.513 kb on + strand, within deoD at 4619.541 kb on + strand, within deoD at 4619.564 kb on - strand at 4619.564 kb on - strand at 4619.593 kb on + strand at 4619.899 kb on + strand at 4619.899 kb on + strand at 4619.929 kb on + strand, within yjjJ at 4619.942 kb on + strand, within yjjJ at 4619.947 kb on + strand, within yjjJ at 4619.947 kb on + strand, within yjjJ at 4619.964 kb on + strand, within yjjJ at 4619.972 kb on - strand, within yjjJ at 4620.029 kb on + strand, within yjjJ at 4620.114 kb on - strand, within yjjJ at 4620.193 kb on - strand, within yjjJ at 4620.217 kb on + strand, within yjjJ at 4620.305 kb on - strand, within yjjJ at 4620.335 kb on - strand, within yjjJ at 4620.350 kb on - strand, within yjjJ at 4620.350 kb on - strand, within yjjJ at 4620.455 kb on - strand, within yjjJ at 4620.484 kb on - strand, within yjjJ at 4620.581 kb on - strand, within yjjJ at 4620.583 kb on + strand, within yjjJ at 4620.608 kb on - strand, within yjjJ at 4620.627 kb on - strand, within yjjJ at 4620.632 kb on - strand, within yjjJ at 4620.677 kb on + strand, within yjjJ at 4620.688 kb on + strand, within yjjJ at 4620.835 kb on - strand, within yjjJ at 4620.883 kb on - strand, within yjjJ at 4620.954 kb on + strand, within yjjJ at 4620.993 kb on + strand at 4621.019 kb on - strand at 4621.117 kb on - strand at 4621.117 kb on - strand at 4621.124 kb on + strand at 4621.128 kb on + strand at 4621.128 kb on + strand at 4621.232 kb on + strand, within lplA at 4621.240 kb on - strand, within lplA at 4621.240 kb on - strand, within lplA at 4621.240 kb on - strand, within lplA at 4621.287 kb on - strand, within lplA at 4621.298 kb on + strand, within lplA at 4621.298 kb on + strand, within lplA at 4621.301 kb on - strand, within lplA at 4621.350 kb on - strand, within lplA at 4621.350 kb on - strand, within lplA at 4621.457 kb on + strand, within lplA at 4621.457 kb on + strand, within lplA at 4621.491 kb on + strand, within lplA at 4621.501 kb on + strand, within lplA at 4621.549 kb on + strand, within lplA at 4621.549 kb on + strand, within lplA at 4621.592 kb on + strand, within lplA at 4621.674 kb on - strand, within lplA at 4621.674 kb on - strand, within lplA at 4621.869 kb on + strand, within lplA at 4621.884 kb on - strand, within lplA at 4621.946 kb on - strand, within lplA at 4621.946 kb on - strand, within lplA at 4621.952 kb on - strand, within lplA at 4621.957 kb on - strand, within lplA at 4621.970 kb on + strand, within lplA at 4621.970 kb on + strand, within lplA at 4621.976 kb on - strand, within lplA at 4622.011 kb on - strand, within lplA at 4622.011 kb on - strand, within lplA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 4,618,851 - -0.7 4,618,864 + -0.1 4,618,872 - -0.9 4,618,977 - -0.4 4,618,991 - deoD b4384 0.12 -1.2 4,619,050 - deoD b4384 0.20 +0.1 4,619,050 - deoD b4384 0.20 -0.4 4,619,089 + deoD b4384 0.25 -1.1 4,619,094 + deoD b4384 0.26 -1.5 4,619,094 + deoD b4384 0.26 -1.0 4,619,104 + deoD b4384 0.28 +1.3 4,619,104 + deoD b4384 0.28 -1.6 4,619,109 + deoD b4384 0.28 -0.2 4,619,117 - deoD b4384 0.29 -2.2 4,619,119 + deoD b4384 0.30 -0.4 4,619,119 + deoD b4384 0.30 -0.2 4,619,128 + deoD b4384 0.31 -0.1 4,619,131 + deoD b4384 0.31 +0.1 4,619,198 + deoD b4384 0.41 -0.7 4,619,223 + deoD b4384 0.44 -1.9 4,619,272 + deoD b4384 0.51 +0.0 4,619,279 + deoD b4384 0.52 -1.4 4,619,298 + deoD b4384 0.54 +0.6 4,619,298 + deoD b4384 0.54 -0.8 4,619,316 - deoD b4384 0.57 -1.7 4,619,335 + deoD b4384 0.60 -0.9 4,619,335 + deoD b4384 0.60 +0.8 4,619,336 - deoD b4384 0.60 -0.6 4,619,340 + deoD b4384 0.60 -0.5 4,619,355 + deoD b4384 0.62 -1.0 4,619,375 - deoD b4384 0.65 -0.5 4,619,375 - deoD b4384 0.65 -0.4 4,619,382 + deoD b4384 0.66 +0.1 4,619,382 + deoD b4384 0.66 +0.4 4,619,385 - deoD b4384 0.67 +0.4 4,619,385 - deoD b4384 0.67 +0.4 4,619,390 - deoD b4384 0.67 +1.0 4,619,390 - deoD b4384 0.67 +0.9 4,619,512 + deoD b4384 0.84 -1.0 4,619,513 + deoD b4384 0.84 -0.4 4,619,513 + deoD b4384 0.84 +1.7 4,619,513 + deoD b4384 0.84 +0.2 4,619,541 + deoD b4384 0.88 -1.4 4,619,564 - -0.7 4,619,564 - -0.7 4,619,593 + -1.5 4,619,899 + -1.0 4,619,899 + +0.8 4,619,929 + yjjJ b4385 0.10 -0.5 4,619,942 + yjjJ b4385 0.11 +1.4 4,619,947 + yjjJ b4385 0.12 +0.8 4,619,947 + yjjJ b4385 0.12 +0.6 4,619,964 + yjjJ b4385 0.13 +0.2 4,619,972 - yjjJ b4385 0.14 +1.2 4,620,029 + yjjJ b4385 0.18 +0.7 4,620,114 - yjjJ b4385 0.24 +0.9 4,620,193 - yjjJ b4385 0.30 +0.9 4,620,217 + yjjJ b4385 0.32 +1.2 4,620,305 - yjjJ b4385 0.39 +0.5 4,620,335 - yjjJ b4385 0.41 +0.7 4,620,350 - yjjJ b4385 0.42 +1.4 4,620,350 - yjjJ b4385 0.42 +0.5 4,620,455 - yjjJ b4385 0.50 +1.2 4,620,484 - yjjJ b4385 0.52 -0.2 4,620,581 - yjjJ b4385 0.59 -0.2 4,620,583 + yjjJ b4385 0.59 -0.3 4,620,608 - yjjJ b4385 0.61 +1.4 4,620,627 - yjjJ b4385 0.63 -0.1 4,620,632 - yjjJ b4385 0.63 +0.1 4,620,677 + yjjJ b4385 0.66 +0.5 4,620,688 + yjjJ b4385 0.67 +0.6 4,620,835 - yjjJ b4385 0.78 +1.1 4,620,883 - yjjJ b4385 0.82 +1.4 4,620,954 + yjjJ b4385 0.87 +0.5 4,620,993 + +0.1 4,621,019 - -0.1 4,621,117 - +0.6 4,621,117 - +0.4 4,621,124 + -0.7 4,621,128 + +1.2 4,621,128 + +0.1 4,621,232 + lplA b4386 0.11 +0.2 4,621,240 - lplA b4386 0.11 +0.6 4,621,240 - lplA b4386 0.11 +0.0 4,621,240 - lplA b4386 0.11 -0.4 4,621,287 - lplA b4386 0.16 +0.9 4,621,298 + lplA b4386 0.17 -0.5 4,621,298 + lplA b4386 0.17 +1.3 4,621,301 - lplA b4386 0.17 +0.1 4,621,350 - lplA b4386 0.22 -0.7 4,621,350 - lplA b4386 0.22 +1.6 4,621,457 + lplA b4386 0.33 +1.4 4,621,457 + lplA b4386 0.33 -0.7 4,621,491 + lplA b4386 0.36 -0.1 4,621,501 + lplA b4386 0.37 +0.9 4,621,549 + lplA b4386 0.42 -2.2 4,621,549 + lplA b4386 0.42 -2.5 4,621,592 + lplA b4386 0.46 +1.7 4,621,674 - lplA b4386 0.54 +1.5 4,621,674 - lplA b4386 0.54 -0.1 4,621,869 + lplA b4386 0.73 +0.9 4,621,884 - lplA b4386 0.75 +0.4 4,621,946 - lplA b4386 0.81 -0.2 4,621,946 - lplA b4386 0.81 +0.5 4,621,952 - lplA b4386 0.81 -1.1 4,621,957 - lplA b4386 0.82 +0.3 4,621,970 + lplA b4386 0.83 -1.2 4,621,970 + lplA b4386 0.83 +0.4 4,621,976 - lplA b4386 0.84 -1.5 4,622,011 - lplA b4386 0.87 +0.7 4,622,011 - lplA b4386 0.87 +0.2
Or see this region's nucleotide sequence