Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt msrA and ytfM are separated by 205 nucleotides ytfM and ytfN overlap by 4 nucleotides
b4219: msrA - methionine sulfoxide reductase A (NCBI), at 4,439,561 to 4,440,199
msrA
b4220: ytfM - predicted outer membrane protein and surface antigen (NCBI), at 4,440,405 to 4,442,138
ytfM
b4221: ytfN - hypothetical protein (NCBI), at 4,442,135 to 4,445,914
ytfN
Position (kb)
4440
4441
4442
4443 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4439.431 kb on - strand at 4439.431 kb on - strand at 4439.466 kb on - strand at 4439.688 kb on + strand, within msrA at 4439.739 kb on + strand, within msrA at 4439.759 kb on + strand, within msrA at 4439.759 kb on + strand, within msrA at 4439.764 kb on + strand, within msrA at 4439.872 kb on + strand, within msrA at 4439.937 kb on - strand, within msrA at 4439.937 kb on - strand, within msrA at 4440.009 kb on + strand, within msrA at 4440.090 kb on + strand, within msrA at 4440.120 kb on - strand, within msrA at 4440.131 kb on - strand, within msrA at 4440.143 kb on - strand at 4440.143 kb on - strand at 4440.279 kb on - strand at 4440.331 kb on + strand at 4440.331 kb on + strand at 4440.430 kb on + strand at 4440.435 kb on + strand at 4440.435 kb on + strand at 4440.435 kb on + strand at 4440.483 kb on - strand at 4440.483 kb on - strand at 4440.485 kb on - strand at 4440.582 kb on + strand, within ytfM at 4440.582 kb on - strand, within ytfM at 4440.675 kb on - strand, within ytfM at 4440.681 kb on + strand, within ytfM at 4440.698 kb on + strand, within ytfM at 4440.740 kb on + strand, within ytfM at 4440.741 kb on - strand, within ytfM at 4440.741 kb on - strand, within ytfM at 4440.770 kb on - strand, within ytfM at 4440.770 kb on - strand, within ytfM at 4440.798 kb on + strand, within ytfM at 4440.810 kb on + strand, within ytfM at 4440.810 kb on + strand, within ytfM at 4440.810 kb on + strand, within ytfM at 4440.810 kb on + strand, within ytfM at 4440.810 kb on + strand, within ytfM at 4440.818 kb on - strand, within ytfM at 4440.861 kb on + strand, within ytfM at 4440.864 kb on - strand, within ytfM at 4440.864 kb on - strand, within ytfM at 4440.935 kb on + strand, within ytfM at 4440.935 kb on + strand, within ytfM at 4440.948 kb on + strand, within ytfM at 4440.973 kb on + strand, within ytfM at 4440.976 kb on - strand, within ytfM at 4440.989 kb on + strand, within ytfM at 4440.996 kb on - strand, within ytfM at 4441.096 kb on + strand, within ytfM at 4441.144 kb on - strand, within ytfM at 4441.234 kb on - strand, within ytfM at 4441.234 kb on - strand, within ytfM at 4441.292 kb on + strand, within ytfM at 4441.306 kb on + strand, within ytfM at 4441.382 kb on + strand, within ytfM at 4441.406 kb on + strand, within ytfM at 4441.406 kb on + strand, within ytfM at 4441.416 kb on + strand, within ytfM at 4441.424 kb on - strand, within ytfM at 4441.441 kb on + strand, within ytfM at 4441.441 kb on + strand, within ytfM at 4441.442 kb on + strand, within ytfM at 4441.456 kb on + strand, within ytfM at 4441.458 kb on + strand, within ytfM at 4441.495 kb on + strand, within ytfM at 4441.503 kb on - strand, within ytfM at 4441.521 kb on - strand, within ytfM at 4441.559 kb on + strand, within ytfM at 4441.564 kb on + strand, within ytfM at 4441.564 kb on + strand, within ytfM at 4441.567 kb on - strand, within ytfM at 4441.614 kb on + strand, within ytfM at 4441.614 kb on + strand, within ytfM at 4441.639 kb on + strand, within ytfM at 4441.639 kb on + strand, within ytfM at 4441.653 kb on + strand, within ytfM at 4441.657 kb on - strand, within ytfM at 4441.657 kb on - strand, within ytfM at 4441.657 kb on - strand, within ytfM at 4441.717 kb on + strand, within ytfM at 4441.727 kb on - strand, within ytfM at 4441.828 kb on + strand, within ytfM at 4441.933 kb on - strand, within ytfM at 4441.933 kb on - strand, within ytfM at 4441.969 kb on - strand at 4441.969 kb on - strand at 4442.033 kb on - strand at 4442.108 kb on + strand at 4442.183 kb on + strand at 4442.201 kb on - strand at 4442.225 kb on + strand at 4442.262 kb on + strand at 4442.262 kb on + strand at 4442.264 kb on - strand at 4442.303 kb on + strand at 4442.303 kb on + strand at 4442.312 kb on - strand at 4442.400 kb on + strand at 4442.448 kb on - strand at 4442.448 kb on - strand at 4442.469 kb on + strand at 4442.469 kb on + strand at 4442.512 kb on - strand at 4442.515 kb on + strand, within ytfN at 4442.535 kb on + strand, within ytfN at 4442.535 kb on + strand, within ytfN at 4442.542 kb on - strand, within ytfN at 4442.543 kb on - strand, within ytfN at 4442.544 kb on + strand, within ytfN at 4442.544 kb on + strand, within ytfN at 4442.549 kb on + strand, within ytfN at 4442.557 kb on - strand, within ytfN at 4442.557 kb on - strand, within ytfN at 4442.557 kb on - strand, within ytfN at 4442.571 kb on + strand, within ytfN at 4442.571 kb on + strand, within ytfN at 4442.594 kb on - strand, within ytfN at 4442.634 kb on + strand, within ytfN at 4442.634 kb on + strand, within ytfN at 4442.704 kb on + strand, within ytfN at 4442.704 kb on + strand, within ytfN at 4442.818 kb on - strand, within ytfN at 4442.853 kb on - strand, within ytfN at 4442.853 kb on - strand, within ytfN at 4442.855 kb on + strand, within ytfN at 4442.872 kb on + strand, within ytfN at 4442.890 kb on + strand, within ytfN at 4442.890 kb on + strand, within ytfN at 4442.912 kb on - strand, within ytfN at 4443.017 kb on + strand, within ytfN at 4443.017 kb on + strand, within ytfN at 4443.031 kb on + strand, within ytfN at 4443.033 kb on + strand, within ytfN at 4443.041 kb on - strand, within ytfN at 4443.041 kb on - strand, within ytfN at 4443.041 kb on - strand, within ytfN at 4443.041 kb on - strand, within ytfN at 4443.053 kb on - strand, within ytfN at 4443.053 kb on - strand, within ytfN at 4443.106 kb on + strand, within ytfN
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 4,439,431 - -0.7 4,439,431 - -1.1 4,439,466 - -0.9 4,439,688 + msrA b4219 0.20 -0.6 4,439,739 + msrA b4219 0.28 -1.2 4,439,759 + msrA b4219 0.31 +0.9 4,439,759 + msrA b4219 0.31 -0.3 4,439,764 + msrA b4219 0.32 +0.0 4,439,872 + msrA b4219 0.49 -0.9 4,439,937 - msrA b4219 0.59 -0.3 4,439,937 - msrA b4219 0.59 -0.2 4,440,009 + msrA b4219 0.70 -0.8 4,440,090 + msrA b4219 0.83 -1.9 4,440,120 - msrA b4219 0.87 -0.0 4,440,131 - msrA b4219 0.89 +0.2 4,440,143 - -2.0 4,440,143 - +1.8 4,440,279 - +0.0 4,440,331 + +1.1 4,440,331 + +0.7 4,440,430 + +1.5 4,440,435 + +0.7 4,440,435 + -0.0 4,440,435 + +0.7 4,440,483 - +0.9 4,440,483 - +1.4 4,440,485 - +1.1 4,440,582 + ytfM b4220 0.10 +1.4 4,440,582 - ytfM b4220 0.10 +0.8 4,440,675 - ytfM b4220 0.16 +0.7 4,440,681 + ytfM b4220 0.16 +1.4 4,440,698 + ytfM b4220 0.17 +0.4 4,440,740 + ytfM b4220 0.19 +1.6 4,440,741 - ytfM b4220 0.19 +0.7 4,440,741 - ytfM b4220 0.19 +0.7 4,440,770 - ytfM b4220 0.21 +1.2 4,440,770 - ytfM b4220 0.21 +1.5 4,440,798 + ytfM b4220 0.23 +1.5 4,440,810 + ytfM b4220 0.23 +0.1 4,440,810 + ytfM b4220 0.23 +1.6 4,440,810 + ytfM b4220 0.23 +1.5 4,440,810 + ytfM b4220 0.23 -0.5 4,440,810 + ytfM b4220 0.23 +0.7 4,440,818 - ytfM b4220 0.24 +0.8 4,440,861 + ytfM b4220 0.26 +1.6 4,440,864 - ytfM b4220 0.26 +2.8 4,440,864 - ytfM b4220 0.26 +0.8 4,440,935 + ytfM b4220 0.31 +0.6 4,440,935 + ytfM b4220 0.31 +1.9 4,440,948 + ytfM b4220 0.31 +1.5 4,440,973 + ytfM b4220 0.33 +1.0 4,440,976 - ytfM b4220 0.33 +1.0 4,440,989 + ytfM b4220 0.34 +1.3 4,440,996 - ytfM b4220 0.34 +1.6 4,441,096 + ytfM b4220 0.40 +2.2 4,441,144 - ytfM b4220 0.43 +1.7 4,441,234 - ytfM b4220 0.48 +0.9 4,441,234 - ytfM b4220 0.48 +1.3 4,441,292 + ytfM b4220 0.51 +2.1 4,441,306 + ytfM b4220 0.52 +0.8 4,441,382 + ytfM b4220 0.56 +2.1 4,441,406 + ytfM b4220 0.58 +1.4 4,441,406 + ytfM b4220 0.58 +1.8 4,441,416 + ytfM b4220 0.58 +1.7 4,441,424 - ytfM b4220 0.59 -0.2 4,441,441 + ytfM b4220 0.60 +1.8 4,441,441 + ytfM b4220 0.60 +1.3 4,441,442 + ytfM b4220 0.60 +1.0 4,441,456 + ytfM b4220 0.61 +0.7 4,441,458 + ytfM b4220 0.61 +1.5 4,441,495 + ytfM b4220 0.63 +1.3 4,441,503 - ytfM b4220 0.63 +1.5 4,441,521 - ytfM b4220 0.64 +1.7 4,441,559 + ytfM b4220 0.67 +1.5 4,441,564 + ytfM b4220 0.67 +1.2 4,441,564 + ytfM b4220 0.67 +0.9 4,441,567 - ytfM b4220 0.67 +0.5 4,441,614 + ytfM b4220 0.70 +1.0 4,441,614 + ytfM b4220 0.70 +2.3 4,441,639 + ytfM b4220 0.71 +1.6 4,441,639 + ytfM b4220 0.71 +1.6 4,441,653 + ytfM b4220 0.72 +1.0 4,441,657 - ytfM b4220 0.72 +0.3 4,441,657 - ytfM b4220 0.72 +1.8 4,441,657 - ytfM b4220 0.72 +1.4 4,441,717 + ytfM b4220 0.76 +1.1 4,441,727 - ytfM b4220 0.76 +1.9 4,441,828 + ytfM b4220 0.82 +0.9 4,441,933 - ytfM b4220 0.88 +1.1 4,441,933 - ytfM b4220 0.88 +0.9 4,441,969 - +1.7 4,441,969 - -0.7 4,442,033 - +1.1 4,442,108 + +1.7 4,442,183 + +1.4 4,442,201 - +1.5 4,442,225 + +1.0 4,442,262 + +0.4 4,442,262 + +1.6 4,442,264 - +1.4 4,442,303 + +1.0 4,442,303 + +1.3 4,442,312 - +0.8 4,442,400 + +1.2 4,442,448 - +0.4 4,442,448 - +1.9 4,442,469 + +1.5 4,442,469 + +1.7 4,442,512 - +1.3 4,442,515 + ytfN b4221 0.10 +0.5 4,442,535 + ytfN b4221 0.11 +1.1 4,442,535 + ytfN b4221 0.11 +1.7 4,442,542 - ytfN b4221 0.11 +1.2 4,442,543 - ytfN b4221 0.11 +1.2 4,442,544 + ytfN b4221 0.11 +0.7 4,442,544 + ytfN b4221 0.11 +0.4 4,442,549 + ytfN b4221 0.11 +1.4 4,442,557 - ytfN b4221 0.11 +1.6 4,442,557 - ytfN b4221 0.11 +0.9 4,442,557 - ytfN b4221 0.11 +1.5 4,442,571 + ytfN b4221 0.12 +1.9 4,442,571 + ytfN b4221 0.12 +0.5 4,442,594 - ytfN b4221 0.12 +0.7 4,442,634 + ytfN b4221 0.13 +1.9 4,442,634 + ytfN b4221 0.13 -1.8 4,442,704 + ytfN b4221 0.15 +1.9 4,442,704 + ytfN b4221 0.15 +0.7 4,442,818 - ytfN b4221 0.18 +1.4 4,442,853 - ytfN b4221 0.19 +1.5 4,442,853 - ytfN b4221 0.19 +0.8 4,442,855 + ytfN b4221 0.19 +1.0 4,442,872 + ytfN b4221 0.19 +1.8 4,442,890 + ytfN b4221 0.20 +1.0 4,442,890 + ytfN b4221 0.20 +1.4 4,442,912 - ytfN b4221 0.21 +1.6 4,443,017 + ytfN b4221 0.23 +1.2 4,443,017 + ytfN b4221 0.23 +1.1 4,443,031 + ytfN b4221 0.24 -0.3 4,443,033 + ytfN b4221 0.24 +0.9 4,443,041 - ytfN b4221 0.24 +1.1 4,443,041 - ytfN b4221 0.24 +1.8 4,443,041 - ytfN b4221 0.24 +0.9 4,443,041 - ytfN b4221 0.24 +1.7 4,443,053 - ytfN b4221 0.24 +0.9 4,443,053 - ytfN b4221 0.24 +2.9 4,443,106 + ytfN b4221 0.26 +1.7
Or see this region's nucleotide sequence