Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rlmB and yjfI are separated by 126 nucleotides yjfI and yjfJ are separated by 18 nucleotides yjfJ and yjfK are separated by 50 nucleotides
b4180: rlmB - 23S rRNA (Gm2251)-methyltransferase (NCBI), at 4,407,298 to 4,408,029
rlmB
b4181: yjfI - hypothetical protein (NCBI), at 4,408,156 to 4,408,557
yjfI
b4182: yjfJ - predicted transcriptional regulator effector protein (NCBI), at 4,408,576 to 4,409,274
yjfJ
b4183: yjfK - hypothetical protein (NCBI), at 4,409,325 to 4,409,984
yjfK
Position (kb)
4408
4409 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4407.253 kb on + strand at 4407.286 kb on - strand at 4407.328 kb on + strand at 4407.335 kb on + strand at 4407.335 kb on + strand at 4407.336 kb on - strand at 4407.336 kb on - strand at 4407.336 kb on - strand at 4407.336 kb on - strand at 4407.345 kb on - strand at 4407.345 kb on - strand at 4407.346 kb on - strand at 4407.404 kb on + strand, within rlmB at 4407.404 kb on + strand, within rlmB at 4407.501 kb on + strand, within rlmB at 4407.534 kb on - strand, within rlmB at 4407.534 kb on - strand at 4407.704 kb on - strand, within rlmB at 4407.772 kb on - strand, within rlmB at 4407.772 kb on - strand, within rlmB at 4407.776 kb on + strand, within rlmB at 4407.776 kb on + strand, within rlmB at 4407.833 kb on + strand, within rlmB at 4407.842 kb on - strand, within rlmB at 4407.849 kb on - strand, within rlmB at 4407.874 kb on + strand, within rlmB at 4407.876 kb on + strand, within rlmB at 4407.876 kb on + strand, within rlmB at 4407.882 kb on - strand, within rlmB at 4407.889 kb on + strand, within rlmB at 4407.891 kb on - strand, within rlmB at 4407.898 kb on + strand, within rlmB at 4407.906 kb on + strand, within rlmB at 4407.907 kb on + strand, within rlmB at 4407.924 kb on - strand, within rlmB at 4407.941 kb on + strand, within rlmB at 4407.942 kb on - strand, within rlmB at 4407.942 kb on - strand, within rlmB at 4407.951 kb on + strand, within rlmB at 4407.953 kb on - strand, within rlmB at 4407.967 kb on + strand at 4407.967 kb on + strand at 4407.977 kb on + strand at 4408.012 kb on + strand at 4408.012 kb on + strand at 4408.012 kb on - strand at 4408.147 kb on + strand at 4408.152 kb on + strand at 4408.152 kb on + strand at 4408.152 kb on + strand at 4408.179 kb on + strand at 4408.186 kb on + strand at 4408.189 kb on + strand at 4408.193 kb on + strand at 4408.193 kb on + strand at 4408.325 kb on - strand, within yjfI at 4408.337 kb on - strand, within yjfI at 4408.344 kb on - strand, within yjfI at 4408.344 kb on - strand, within yjfI at 4408.344 kb on - strand, within yjfI at 4408.344 kb on - strand, within yjfI at 4408.345 kb on - strand, within yjfI at 4408.406 kb on - strand, within yjfI at 4408.406 kb on - strand, within yjfI at 4408.411 kb on - strand, within yjfI at 4408.413 kb on - strand, within yjfI at 4408.413 kb on - strand, within yjfI at 4408.413 kb on - strand, within yjfI at 4408.413 kb on - strand, within yjfI at 4408.413 kb on - strand, within yjfI at 4408.423 kb on - strand, within yjfI at 4408.425 kb on - strand, within yjfI at 4408.442 kb on - strand, within yjfI at 4408.469 kb on - strand, within yjfI at 4408.500 kb on - strand, within yjfI at 4408.500 kb on - strand, within yjfI at 4408.512 kb on - strand, within yjfI at 4408.638 kb on - strand at 4408.662 kb on - strand, within yjfJ at 4408.662 kb on - strand, within yjfJ at 4408.666 kb on + strand, within yjfJ at 4408.666 kb on + strand, within yjfJ at 4408.668 kb on - strand, within yjfJ at 4408.668 kb on - strand, within yjfJ at 4408.670 kb on - strand, within yjfJ at 4408.672 kb on - strand, within yjfJ at 4408.672 kb on - strand, within yjfJ at 4408.694 kb on - strand, within yjfJ at 4408.694 kb on - strand, within yjfJ at 4408.777 kb on - strand, within yjfJ at 4408.779 kb on + strand, within yjfJ at 4408.779 kb on + strand, within yjfJ at 4408.788 kb on - strand, within yjfJ at 4408.807 kb on + strand, within yjfJ at 4408.813 kb on - strand, within yjfJ at 4408.879 kb on - strand, within yjfJ at 4408.894 kb on + strand, within yjfJ at 4408.894 kb on + strand, within yjfJ at 4408.897 kb on + strand, within yjfJ at 4408.897 kb on + strand, within yjfJ at 4408.931 kb on - strand, within yjfJ at 4408.960 kb on - strand, within yjfJ at 4408.986 kb on + strand, within yjfJ at 4409.015 kb on - strand, within yjfJ at 4409.022 kb on - strand, within yjfJ at 4409.032 kb on - strand, within yjfJ at 4409.032 kb on - strand, within yjfJ at 4409.115 kb on + strand, within yjfJ at 4409.115 kb on + strand, within yjfJ at 4409.119 kb on + strand, within yjfJ at 4409.131 kb on + strand, within yjfJ at 4409.131 kb on + strand, within yjfJ at 4409.131 kb on + strand, within yjfJ at 4409.138 kb on - strand, within yjfJ at 4409.140 kb on - strand, within yjfJ at 4409.141 kb on + strand, within yjfJ at 4409.151 kb on + strand, within yjfJ at 4409.159 kb on - strand, within yjfJ at 4409.160 kb on + strand, within yjfJ at 4409.160 kb on + strand, within yjfJ at 4409.160 kb on + strand, within yjfJ at 4409.161 kb on + strand, within yjfJ at 4409.229 kb on + strand at 4409.243 kb on + strand at 4409.257 kb on + strand at 4409.348 kb on + strand at 4409.395 kb on - strand, within yjfK at 4409.402 kb on + strand, within yjfK at 4409.402 kb on + strand, within yjfK at 4409.405 kb on - strand, within yjfK at 4409.425 kb on - strand, within yjfK at 4409.425 kb on - strand, within yjfK at 4409.459 kb on - strand, within yjfK at 4409.498 kb on + strand, within yjfK at 4409.521 kb on - strand, within yjfK at 4409.537 kb on + strand, within yjfK at 4409.537 kb on + strand, within yjfK at 4409.551 kb on - strand, within yjfK
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 4,407,253 + -1.5 4,407,286 - -0.6 4,407,328 + -0.9 4,407,335 + -0.7 4,407,335 + -3.2 4,407,336 - +0.7 4,407,336 - -0.4 4,407,336 - +0.2 4,407,336 - -1.8 4,407,345 - -0.0 4,407,345 - +0.1 4,407,346 - -0.9 4,407,404 + rlmB b4180 0.14 -1.0 4,407,404 + rlmB b4180 0.14 -1.6 4,407,501 + rlmB b4180 0.28 -1.6 4,407,534 - rlmB b4180 0.32 +0.4 4,407,534 - +0.6 4,407,704 - rlmB b4180 0.55 -1.6 4,407,772 - rlmB b4180 0.65 +1.4 4,407,772 - rlmB b4180 0.65 -0.4 4,407,776 + rlmB b4180 0.65 -1.4 4,407,776 + rlmB b4180 0.65 +0.2 4,407,833 + rlmB b4180 0.73 +1.1 4,407,842 - rlmB b4180 0.74 -0.9 4,407,849 - rlmB b4180 0.75 -1.0 4,407,874 + rlmB b4180 0.79 +0.5 4,407,876 + rlmB b4180 0.79 +0.7 4,407,876 + rlmB b4180 0.79 -0.6 4,407,882 - rlmB b4180 0.80 -0.6 4,407,889 + rlmB b4180 0.81 -1.0 4,407,891 - rlmB b4180 0.81 -1.0 4,407,898 + rlmB b4180 0.82 -0.4 4,407,906 + rlmB b4180 0.83 -0.3 4,407,907 + rlmB b4180 0.83 -1.0 4,407,924 - rlmB b4180 0.86 -0.2 4,407,941 + rlmB b4180 0.88 -0.2 4,407,942 - rlmB b4180 0.88 +2.0 4,407,942 - rlmB b4180 0.88 +0.4 4,407,951 + rlmB b4180 0.89 +0.9 4,407,953 - rlmB b4180 0.89 +2.0 4,407,967 + -1.2 4,407,967 + -1.4 4,407,977 + -0.7 4,408,012 + -0.4 4,408,012 + -0.1 4,408,012 - +1.9 4,408,147 + +0.9 4,408,152 + +0.9 4,408,152 + +0.9 4,408,152 + +1.9 4,408,179 + +2.9 4,408,186 + +1.9 4,408,189 + +1.9 4,408,193 + +1.9 4,408,193 + +1.9 4,408,325 - yjfI b4181 0.42 +1.9 4,408,337 - yjfI b4181 0.45 -0.4 4,408,344 - yjfI b4181 0.47 +1.9 4,408,344 - yjfI b4181 0.47 -0.4 4,408,344 - yjfI b4181 0.47 +0.4 4,408,344 - yjfI b4181 0.47 +0.4 4,408,345 - yjfI b4181 0.47 +3.2 4,408,406 - yjfI b4181 0.62 +1.9 4,408,406 - yjfI b4181 0.62 +1.9 4,408,411 - yjfI b4181 0.63 -0.4 4,408,413 - yjfI b4181 0.64 +0.4 4,408,413 - yjfI b4181 0.64 -0.6 4,408,413 - yjfI b4181 0.64 -1.1 4,408,413 - yjfI b4181 0.64 -0.4 4,408,413 - yjfI b4181 0.64 +0.4 4,408,423 - yjfI b4181 0.66 +0.4 4,408,425 - yjfI b4181 0.67 -0.5 4,408,442 - yjfI b4181 0.71 +1.9 4,408,469 - yjfI b4181 0.78 -0.6 4,408,500 - yjfI b4181 0.86 +0.9 4,408,500 - yjfI b4181 0.86 +0.9 4,408,512 - yjfI b4181 0.89 +1.9 4,408,638 - -2.3 4,408,662 - yjfJ b4182 0.12 -1.5 4,408,662 - yjfJ b4182 0.12 +0.4 4,408,666 + yjfJ b4182 0.13 +1.9 4,408,666 + yjfJ b4182 0.13 +1.9 4,408,668 - yjfJ b4182 0.13 -0.4 4,408,668 - yjfJ b4182 0.13 -0.6 4,408,670 - yjfJ b4182 0.13 +0.1 4,408,672 - yjfJ b4182 0.14 -1.8 4,408,672 - yjfJ b4182 0.14 -1.8 4,408,694 - yjfJ b4182 0.17 -0.8 4,408,694 - yjfJ b4182 0.17 +1.9 4,408,777 - yjfJ b4182 0.29 +0.9 4,408,779 + yjfJ b4182 0.29 -0.4 4,408,779 + yjfJ b4182 0.29 -0.9 4,408,788 - yjfJ b4182 0.30 -1.8 4,408,807 + yjfJ b4182 0.33 +1.3 4,408,813 - yjfJ b4182 0.34 -0.2 4,408,879 - yjfJ b4182 0.43 -0.1 4,408,894 + yjfJ b4182 0.45 -0.8 4,408,894 + yjfJ b4182 0.45 -1.5 4,408,897 + yjfJ b4182 0.46 +0.1 4,408,897 + yjfJ b4182 0.46 -0.1 4,408,931 - yjfJ b4182 0.51 -1.6 4,408,960 - yjfJ b4182 0.55 -0.2 4,408,986 + yjfJ b4182 0.59 +1.0 4,409,015 - yjfJ b4182 0.63 -0.2 4,409,022 - yjfJ b4182 0.64 -0.1 4,409,032 - yjfJ b4182 0.65 +0.5 4,409,032 - yjfJ b4182 0.65 +0.7 4,409,115 + yjfJ b4182 0.77 -2.4 4,409,115 + yjfJ b4182 0.77 -1.7 4,409,119 + yjfJ b4182 0.78 -1.0 4,409,131 + yjfJ b4182 0.79 +1.2 4,409,131 + yjfJ b4182 0.79 +0.3 4,409,131 + yjfJ b4182 0.79 -0.7 4,409,138 - yjfJ b4182 0.80 -2.8 4,409,140 - yjfJ b4182 0.81 +0.3 4,409,141 + yjfJ b4182 0.81 -0.4 4,409,151 + yjfJ b4182 0.82 -0.5 4,409,159 - yjfJ b4182 0.83 +0.6 4,409,160 + yjfJ b4182 0.84 -1.3 4,409,160 + yjfJ b4182 0.84 -1.1 4,409,160 + yjfJ b4182 0.84 -2.7 4,409,161 + yjfJ b4182 0.84 -0.3 4,409,229 + -0.2 4,409,243 + -0.6 4,409,257 + -1.0 4,409,348 + -1.4 4,409,395 - yjfK b4183 0.11 +0.8 4,409,402 + yjfK b4183 0.12 +1.0 4,409,402 + yjfK b4183 0.12 +0.2 4,409,405 - yjfK b4183 0.12 +0.7 4,409,425 - yjfK b4183 0.15 -0.4 4,409,425 - yjfK b4183 0.15 -0.4 4,409,459 - yjfK b4183 0.20 -0.2 4,409,498 + yjfK b4183 0.26 -1.0 4,409,521 - yjfK b4183 0.30 -0.3 4,409,537 + yjfK b4183 0.32 +1.5 4,409,537 + yjfK b4183 0.32 -1.0 4,409,551 - yjfK b4183 0.34 +0.4
Or see this region's nucleotide sequence