Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tsgA and nirB are separated by 261 nucleotides nirB and nirD overlap by 4 nucleotides nirD and nirC are separated by 125 nucleotides
b3364: tsgA - hypothetical protein (NCBI), at 3,490,590 to 3,491,771
tsgA
b3365: nirB - nitrite reductase, large subunit, NAD(P)H-binding (NCBI), at 3,492,033 to 3,494,576
nirB
b3366: nirD - nitrite reductase small subunit (NCBI), at 3,494,573 to 3,494,899
nirD
b3367: nirC - nitrite transporter (NCBI), at 3,495,025 to 3,495,831
nirC
Position (kb)
3492
3493
3494
3495 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3491.065 kb on + strand, within tsgA at 3491.065 kb on + strand, within tsgA at 3491.068 kb on - strand, within tsgA at 3491.068 kb on - strand, within tsgA at 3491.073 kb on - strand, within tsgA at 3491.073 kb on - strand, within tsgA at 3491.073 kb on - strand, within tsgA at 3491.075 kb on + strand, within tsgA at 3491.075 kb on + strand, within tsgA at 3491.075 kb on + strand, within tsgA at 3491.083 kb on - strand, within tsgA at 3491.179 kb on + strand, within tsgA at 3491.181 kb on + strand, within tsgA at 3491.234 kb on - strand, within tsgA at 3491.253 kb on - strand, within tsgA at 3491.253 kb on - strand, within tsgA at 3491.263 kb on - strand, within tsgA at 3491.274 kb on + strand, within tsgA at 3491.274 kb on + strand, within tsgA at 3491.280 kb on + strand, within tsgA at 3491.280 kb on + strand, within tsgA at 3491.304 kb on + strand, within tsgA at 3491.315 kb on + strand, within tsgA at 3491.367 kb on - strand, within tsgA at 3491.367 kb on - strand, within tsgA at 3491.375 kb on - strand, within tsgA at 3491.382 kb on - strand, within tsgA at 3491.402 kb on - strand, within tsgA at 3491.420 kb on + strand, within tsgA at 3491.420 kb on + strand, within tsgA at 3491.468 kb on + strand, within tsgA at 3491.470 kb on + strand, within tsgA at 3491.472 kb on + strand, within tsgA at 3491.517 kb on + strand, within tsgA at 3491.517 kb on + strand, within tsgA at 3491.517 kb on + strand, within tsgA at 3491.526 kb on + strand, within tsgA at 3491.540 kb on + strand, within tsgA at 3491.542 kb on + strand, within tsgA at 3491.542 kb on + strand, within tsgA at 3491.550 kb on - strand, within tsgA at 3491.550 kb on - strand, within tsgA at 3491.550 kb on - strand, within tsgA at 3491.554 kb on + strand, within tsgA at 3491.555 kb on - strand, within tsgA at 3491.603 kb on + strand, within tsgA at 3491.603 kb on + strand, within tsgA at 3491.621 kb on - strand, within tsgA at 3491.621 kb on - strand, within tsgA at 3491.646 kb on + strand, within tsgA at 3491.646 kb on + strand, within tsgA at 3491.646 kb on + strand, within tsgA at 3491.666 kb on - strand at 3491.675 kb on - strand at 3491.726 kb on - strand at 3491.726 kb on - strand at 3491.726 kb on - strand at 3491.747 kb on + strand at 3491.747 kb on + strand at 3491.755 kb on - strand at 3491.755 kb on - strand at 3491.801 kb on - strand at 3491.814 kb on + strand at 3491.814 kb on - strand at 3491.841 kb on + strand at 3491.841 kb on + strand at 3491.993 kb on + strand at 3491.993 kb on + strand at 3492.065 kb on + strand at 3492.065 kb on + strand at 3492.065 kb on + strand at 3492.073 kb on - strand at 3492.159 kb on + strand at 3492.159 kb on + strand at 3492.159 kb on + strand at 3492.167 kb on - strand at 3492.176 kb on - strand at 3492.198 kb on - strand at 3492.220 kb on - strand at 3492.275 kb on + strand at 3492.279 kb on + strand at 3492.316 kb on + strand, within nirB at 3492.319 kb on - strand, within nirB at 3492.352 kb on - strand, within nirB at 3492.355 kb on - strand, within nirB at 3492.361 kb on + strand, within nirB at 3492.424 kb on + strand, within nirB at 3492.424 kb on + strand, within nirB at 3492.434 kb on - strand, within nirB at 3492.444 kb on + strand, within nirB at 3492.495 kb on - strand, within nirB at 3492.495 kb on - strand, within nirB at 3492.574 kb on + strand, within nirB at 3492.574 kb on + strand, within nirB at 3492.599 kb on - strand, within nirB at 3492.647 kb on - strand, within nirB at 3492.677 kb on - strand, within nirB at 3492.680 kb on - strand, within nirB at 3492.690 kb on - strand, within nirB at 3492.765 kb on - strand, within nirB at 3492.765 kb on - strand, within nirB at 3492.792 kb on + strand, within nirB at 3492.793 kb on + strand, within nirB at 3492.822 kb on + strand, within nirB at 3492.822 kb on + strand, within nirB at 3492.918 kb on + strand, within nirB at 3492.918 kb on + strand, within nirB at 3492.952 kb on - strand, within nirB at 3492.959 kb on - strand, within nirB at 3492.970 kb on + strand, within nirB at 3492.979 kb on + strand, within nirB at 3493.051 kb on - strand, within nirB at 3493.053 kb on - strand, within nirB at 3493.141 kb on + strand, within nirB at 3493.146 kb on + strand, within nirB at 3493.149 kb on - strand, within nirB at 3493.149 kb on - strand, within nirB at 3493.179 kb on + strand, within nirB at 3493.265 kb on + strand, within nirB at 3493.284 kb on + strand, within nirB at 3493.292 kb on - strand, within nirB at 3493.299 kb on + strand, within nirB at 3493.299 kb on + strand, within nirB at 3493.312 kb on + strand, within nirB at 3493.312 kb on + strand, within nirB at 3493.347 kb on + strand, within nirB at 3493.352 kb on - strand, within nirB at 3493.358 kb on + strand, within nirB at 3493.364 kb on - strand, within nirB at 3493.364 kb on - strand, within nirB at 3493.414 kb on + strand, within nirB at 3493.422 kb on + strand, within nirB at 3493.422 kb on + strand, within nirB at 3493.430 kb on - strand, within nirB at 3493.430 kb on - strand, within nirB at 3493.432 kb on + strand, within nirB at 3493.481 kb on + strand, within nirB at 3493.582 kb on - strand, within nirB at 3493.591 kb on + strand, within nirB at 3493.599 kb on - strand, within nirB at 3493.623 kb on + strand, within nirB at 3493.623 kb on + strand, within nirB at 3493.623 kb on + strand, within nirB at 3493.724 kb on + strand, within nirB at 3493.779 kb on + strand, within nirB at 3493.794 kb on - strand, within nirB at 3493.805 kb on - strand, within nirB at 3493.805 kb on - strand, within nirB at 3493.811 kb on + strand, within nirB at 3493.882 kb on + strand, within nirB at 3493.882 kb on + strand, within nirB at 3493.959 kb on + strand, within nirB at 3493.989 kb on - strand, within nirB at 3494.041 kb on - strand, within nirB at 3494.107 kb on - strand, within nirB at 3494.107 kb on - strand, within nirB at 3494.247 kb on + strand, within nirB at 3494.347 kb on + strand at 3494.395 kb on + strand at 3494.434 kb on + strand at 3494.434 kb on + strand at 3494.452 kb on - strand at 3494.459 kb on + strand at 3494.488 kb on + strand at 3494.499 kb on - strand at 3494.499 kb on - strand at 3494.507 kb on - strand at 3494.507 kb on - strand at 3494.507 kb on - strand at 3494.540 kb on - strand at 3494.548 kb on + strand at 3494.564 kb on - strand at 3494.577 kb on - strand at 3494.577 kb on - strand at 3494.646 kb on - strand, within nirD at 3494.742 kb on - strand, within nirD at 3494.760 kb on + strand, within nirD at 3494.765 kb on + strand, within nirD at 3494.765 kb on + strand, within nirD at 3494.765 kb on + strand, within nirD at 3494.779 kb on - strand, within nirD at 3494.888 kb on - strand at 3494.888 kb on - strand at 3494.939 kb on + strand at 3494.939 kb on + strand at 3494.947 kb on - strand at 3494.947 kb on - strand at 3495.048 kb on + strand at 3495.056 kb on - strand at 3495.062 kb on - strand at 3495.071 kb on + strand at 3495.079 kb on - strand at 3495.113 kb on + strand, within nirC at 3495.113 kb on + strand, within nirC at 3495.123 kb on + strand, within nirC at 3495.124 kb on - strand, within nirC at 3495.124 kb on - strand, within nirC at 3495.129 kb on + strand, within nirC at 3495.129 kb on + strand, within nirC at 3495.131 kb on + strand, within nirC at 3495.148 kb on - strand, within nirC at 3495.148 kb on - strand, within nirC at 3495.148 kb on - strand, within nirC at 3495.148 kb on - strand, within nirC at 3495.182 kb on + strand, within nirC at 3495.195 kb on - strand, within nirC at 3495.197 kb on + strand, within nirC at 3495.197 kb on + strand, within nirC at 3495.251 kb on + strand, within nirC at 3495.251 kb on + strand, within nirC at 3495.251 kb on + strand, within nirC at 3495.259 kb on - strand, within nirC at 3495.271 kb on - strand, within nirC at 3495.308 kb on + strand, within nirC at 3495.308 kb on + strand, within nirC at 3495.311 kb on - strand, within nirC at 3495.311 kb on - strand, within nirC at 3495.353 kb on - strand, within nirC at 3495.358 kb on + strand, within nirC at 3495.388 kb on + strand, within nirC at 3495.388 kb on + strand, within nirC at 3495.388 kb on + strand, within nirC at 3495.388 kb on + strand, within nirC at 3495.388 kb on + strand, within nirC at 3495.396 kb on - strand, within nirC at 3495.491 kb on + strand, within nirC at 3495.535 kb on + strand, within nirC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 3,491,065 + tsgA b3364 0.40 +0.2 3,491,065 + tsgA b3364 0.40 +0.2 3,491,068 - tsgA b3364 0.40 -1.7 3,491,068 - tsgA b3364 0.40 -1.0 3,491,073 - tsgA b3364 0.41 -0.0 3,491,073 - tsgA b3364 0.41 -1.6 3,491,073 - tsgA b3364 0.41 +0.5 3,491,075 + tsgA b3364 0.41 -0.4 3,491,075 + tsgA b3364 0.41 +0.1 3,491,075 + tsgA b3364 0.41 -0.2 3,491,083 - tsgA b3364 0.42 -0.2 3,491,179 + tsgA b3364 0.50 +1.6 3,491,181 + tsgA b3364 0.50 +0.2 3,491,234 - tsgA b3364 0.54 +0.9 3,491,253 - tsgA b3364 0.56 -1.4 3,491,253 - tsgA b3364 0.56 -0.6 3,491,263 - tsgA b3364 0.57 -1.0 3,491,274 + tsgA b3364 0.58 +0.5 3,491,274 + tsgA b3364 0.58 +0.6 3,491,280 + tsgA b3364 0.58 -0.2 3,491,280 + tsgA b3364 0.58 -0.1 3,491,304 + tsgA b3364 0.60 -0.7 3,491,315 + tsgA b3364 0.61 +0.4 3,491,367 - tsgA b3364 0.66 -0.1 3,491,367 - tsgA b3364 0.66 -0.2 3,491,375 - tsgA b3364 0.66 -2.4 3,491,382 - tsgA b3364 0.67 -0.0 3,491,402 - tsgA b3364 0.69 +0.2 3,491,420 + tsgA b3364 0.70 +1.0 3,491,420 + tsgA b3364 0.70 +2.0 3,491,468 + tsgA b3364 0.74 -1.2 3,491,470 + tsgA b3364 0.74 +0.1 3,491,472 + tsgA b3364 0.75 +0.4 3,491,517 + tsgA b3364 0.78 -0.3 3,491,517 + tsgA b3364 0.78 -1.4 3,491,517 + tsgA b3364 0.78 -1.8 3,491,526 + tsgA b3364 0.79 +0.3 3,491,540 + tsgA b3364 0.80 +0.6 3,491,542 + tsgA b3364 0.81 -0.7 3,491,542 + tsgA b3364 0.81 -1.3 3,491,550 - tsgA b3364 0.81 -1.5 3,491,550 - tsgA b3364 0.81 -2.4 3,491,550 - tsgA b3364 0.81 -0.1 3,491,554 + tsgA b3364 0.82 +0.1 3,491,555 - tsgA b3364 0.82 -0.3 3,491,603 + tsgA b3364 0.86 +0.6 3,491,603 + tsgA b3364 0.86 +0.4 3,491,621 - tsgA b3364 0.87 -0.4 3,491,621 - tsgA b3364 0.87 +0.8 3,491,646 + tsgA b3364 0.89 -0.9 3,491,646 + tsgA b3364 0.89 +0.4 3,491,646 + tsgA b3364 0.89 -1.1 3,491,666 - +0.1 3,491,675 - -1.2 3,491,726 - -1.0 3,491,726 - +0.1 3,491,726 - -0.1 3,491,747 + -1.7 3,491,747 + +0.1 3,491,755 - -0.3 3,491,755 - +1.1 3,491,801 - +0.4 3,491,814 + -0.8 3,491,814 - -0.1 3,491,841 + +2.0 3,491,841 + -1.2 3,491,993 + -0.0 3,491,993 + -0.7 3,492,065 + +2.0 3,492,065 + +1.5 3,492,065 + +1.4 3,492,073 - -0.1 3,492,159 + +1.0 3,492,159 + +1.6 3,492,159 + -0.5 3,492,167 - +1.4 3,492,176 - +1.4 3,492,198 - +0.5 3,492,220 - +1.3 3,492,275 + +0.1 3,492,279 + -0.3 3,492,316 + nirB b3365 0.11 +2.3 3,492,319 - nirB b3365 0.11 +0.9 3,492,352 - nirB b3365 0.13 +0.9 3,492,355 - nirB b3365 0.13 +0.8 3,492,361 + nirB b3365 0.13 +1.6 3,492,424 + nirB b3365 0.15 -0.2 3,492,424 + nirB b3365 0.15 +1.2 3,492,434 - nirB b3365 0.16 +1.3 3,492,444 + nirB b3365 0.16 +1.2 3,492,495 - nirB b3365 0.18 +1.0 3,492,495 - nirB b3365 0.18 +0.9 3,492,574 + nirB b3365 0.21 +0.9 3,492,574 + nirB b3365 0.21 +0.9 3,492,599 - nirB b3365 0.22 +0.1 3,492,647 - nirB b3365 0.24 +0.7 3,492,677 - nirB b3365 0.25 +1.1 3,492,680 - nirB b3365 0.25 +1.0 3,492,690 - nirB b3365 0.26 +0.2 3,492,765 - nirB b3365 0.29 +1.1 3,492,765 - nirB b3365 0.29 +1.6 3,492,792 + nirB b3365 0.30 -0.0 3,492,793 + nirB b3365 0.30 +1.6 3,492,822 + nirB b3365 0.31 +0.4 3,492,822 + nirB b3365 0.31 +0.2 3,492,918 + nirB b3365 0.35 +1.0 3,492,918 + nirB b3365 0.35 +0.4 3,492,952 - nirB b3365 0.36 -1.7 3,492,959 - nirB b3365 0.36 -1.1 3,492,970 + nirB b3365 0.37 +0.5 3,492,979 + nirB b3365 0.37 +0.6 3,493,051 - nirB b3365 0.40 +0.8 3,493,053 - nirB b3365 0.40 +1.8 3,493,141 + nirB b3365 0.44 -0.5 3,493,146 + nirB b3365 0.44 +1.2 3,493,149 - nirB b3365 0.44 +0.7 3,493,149 - nirB b3365 0.44 +0.5 3,493,179 + nirB b3365 0.45 -3.2 3,493,265 + nirB b3365 0.48 +1.6 3,493,284 + nirB b3365 0.49 +0.6 3,493,292 - nirB b3365 0.49 +0.3 3,493,299 + nirB b3365 0.50 -1.6 3,493,299 + nirB b3365 0.50 +1.6 3,493,312 + nirB b3365 0.50 -0.8 3,493,312 + nirB b3365 0.50 +0.5 3,493,347 + nirB b3365 0.52 -0.1 3,493,352 - nirB b3365 0.52 +0.1 3,493,358 + nirB b3365 0.52 +1.5 3,493,364 - nirB b3365 0.52 +0.6 3,493,364 - nirB b3365 0.52 +0.3 3,493,414 + nirB b3365 0.54 -0.1 3,493,422 + nirB b3365 0.55 +0.9 3,493,422 + nirB b3365 0.55 -1.2 3,493,430 - nirB b3365 0.55 +0.7 3,493,430 - nirB b3365 0.55 +1.6 3,493,432 + nirB b3365 0.55 -1.8 3,493,481 + nirB b3365 0.57 +0.3 3,493,582 - nirB b3365 0.61 +0.4 3,493,591 + nirB b3365 0.61 +0.5 3,493,599 - nirB b3365 0.62 -0.1 3,493,623 + nirB b3365 0.62 -0.6 3,493,623 + nirB b3365 0.62 -0.9 3,493,623 + nirB b3365 0.62 +0.7 3,493,724 + nirB b3365 0.66 -1.4 3,493,779 + nirB b3365 0.69 -1.5 3,493,794 - nirB b3365 0.69 +1.1 3,493,805 - nirB b3365 0.70 +1.1 3,493,805 - nirB b3365 0.70 +0.9 3,493,811 + nirB b3365 0.70 -0.0 3,493,882 + nirB b3365 0.73 -1.4 3,493,882 + nirB b3365 0.73 -0.9 3,493,959 + nirB b3365 0.76 +0.1 3,493,989 - nirB b3365 0.77 +0.4 3,494,041 - nirB b3365 0.79 +1.4 3,494,107 - nirB b3365 0.82 -0.9 3,494,107 - nirB b3365 0.82 +1.2 3,494,247 + nirB b3365 0.87 +1.0 3,494,347 + -0.2 3,494,395 + -1.1 3,494,434 + +0.7 3,494,434 + +0.1 3,494,452 - +0.1 3,494,459 + -0.8 3,494,488 + +1.7 3,494,499 - +2.1 3,494,499 - +0.8 3,494,507 - -0.5 3,494,507 - +0.8 3,494,507 - +0.7 3,494,540 - -0.0 3,494,548 + +0.8 3,494,564 - -0.2 3,494,577 - +0.4 3,494,577 - -2.4 3,494,646 - nirD b3366 0.22 -1.3 3,494,742 - nirD b3366 0.52 +0.3 3,494,760 + nirD b3366 0.57 -0.8 3,494,765 + nirD b3366 0.59 -1.5 3,494,765 + nirD b3366 0.59 -3.3 3,494,765 + nirD b3366 0.59 -1.2 3,494,779 - nirD b3366 0.63 +0.3 3,494,888 - -0.5 3,494,888 - -1.3 3,494,939 + +0.1 3,494,939 + -0.7 3,494,947 - -0.1 3,494,947 - +0.5 3,495,048 + -2.9 3,495,056 - -0.0 3,495,062 - +0.6 3,495,071 + +0.0 3,495,079 - +0.8 3,495,113 + nirC b3367 0.11 +1.1 3,495,113 + nirC b3367 0.11 +0.8 3,495,123 + nirC b3367 0.12 -0.5 3,495,124 - nirC b3367 0.12 +0.8 3,495,124 - nirC b3367 0.12 +0.2 3,495,129 + nirC b3367 0.13 +0.3 3,495,129 + nirC b3367 0.13 -1.2 3,495,131 + nirC b3367 0.13 -1.2 3,495,148 - nirC b3367 0.15 +2.1 3,495,148 - nirC b3367 0.15 -1.3 3,495,148 - nirC b3367 0.15 -0.8 3,495,148 - nirC b3367 0.15 +1.1 3,495,182 + nirC b3367 0.19 -0.4 3,495,195 - nirC b3367 0.21 -0.5 3,495,197 + nirC b3367 0.21 -1.0 3,495,197 + nirC b3367 0.21 -0.6 3,495,251 + nirC b3367 0.28 +0.8 3,495,251 + nirC b3367 0.28 +0.6 3,495,251 + nirC b3367 0.28 -0.1 3,495,259 - nirC b3367 0.29 -0.6 3,495,271 - nirC b3367 0.30 -0.4 3,495,308 + nirC b3367 0.35 +1.2 3,495,308 + nirC b3367 0.35 -0.3 3,495,311 - nirC b3367 0.35 +0.5 3,495,311 - nirC b3367 0.35 -0.4 3,495,353 - nirC b3367 0.41 +0.5 3,495,358 + nirC b3367 0.41 +0.5 3,495,388 + nirC b3367 0.45 +1.9 3,495,388 + nirC b3367 0.45 -0.1 3,495,388 + nirC b3367 0.45 +0.2 3,495,388 + nirC b3367 0.45 +0.1 3,495,388 + nirC b3367 0.45 -0.2 3,495,396 - nirC b3367 0.46 +0.0 3,495,491 + nirC b3367 0.58 +0.1 3,495,535 + nirC b3367 0.63 -0.2
Or see this region's nucleotide sequence