Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gspF and gspG are separated by 9 nucleotides gspG and gspH are separated by 7 nucleotides gspH and yheH overlap by 4 nucleotides yheH and gspJ overlap by 8 nucleotides
b3327: gspF - general secretory pathway component, cryptic (NCBI), at 3,457,839 to 3,459,035
gspF
b3328: gspG - pseudopilin, cryptic, general secretion pathway (NCBI), at 3,459,045 to 3,459,482
gspG
b3329: gspH - predicted general secretory pathway component, cryptic (NCBI), at 3,459,490 to 3,459,999
gspH
b3330: yheH - putative export protein for general secretion pathway (GSP) (VIMSS), at 3,459,996 to 3,460,373
yheH
b3331: gspJ - predicted general secretory pathway component, cryptic (NCBI), at 3,460,366 to 3,460,953
gspJ
Position (kb)
3459
3460 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3458.048 kb on + strand, within gspF at 3458.067 kb on + strand, within gspF at 3458.067 kb on + strand, within gspF at 3458.074 kb on - strand, within gspF at 3458.085 kb on + strand, within gspF at 3458.093 kb on - strand, within gspF at 3458.113 kb on - strand, within gspF at 3458.141 kb on + strand, within gspF at 3458.218 kb on + strand, within gspF at 3458.223 kb on - strand, within gspF at 3458.223 kb on - strand, within gspF at 3458.223 kb on - strand, within gspF at 3458.223 kb on - strand, within gspF at 3458.223 kb on - strand, within gspF at 3458.223 kb on - strand, within gspF at 3458.243 kb on - strand, within gspF at 3458.243 kb on - strand, within gspF at 3458.324 kb on + strand, within gspF at 3458.324 kb on + strand, within gspF at 3458.324 kb on + strand, within gspF at 3458.338 kb on - strand, within gspF at 3458.338 kb on - strand, within gspF at 3458.348 kb on + strand, within gspF at 3458.348 kb on + strand, within gspF at 3458.353 kb on - strand, within gspF at 3458.358 kb on - strand, within gspF at 3458.406 kb on + strand, within gspF at 3458.420 kb on + strand, within gspF at 3458.420 kb on + strand, within gspF at 3458.428 kb on - strand, within gspF at 3458.453 kb on + strand, within gspF at 3458.475 kb on + strand, within gspF at 3458.505 kb on - strand, within gspF at 3458.521 kb on - strand, within gspF at 3458.521 kb on - strand, within gspF at 3458.632 kb on - strand, within gspF at 3458.632 kb on - strand, within gspF at 3458.638 kb on + strand, within gspF at 3458.663 kb on - strand, within gspF at 3458.668 kb on + strand, within gspF at 3458.671 kb on - strand, within gspF at 3458.687 kb on - strand, within gspF at 3458.687 kb on - strand, within gspF at 3458.689 kb on + strand, within gspF at 3458.711 kb on + strand, within gspF at 3458.817 kb on + strand, within gspF at 3458.817 kb on + strand, within gspF at 3458.822 kb on + strand, within gspF at 3458.822 kb on + strand, within gspF at 3458.823 kb on + strand, within gspF at 3458.878 kb on + strand, within gspF at 3458.889 kb on + strand, within gspF at 3458.899 kb on - strand, within gspF at 3458.909 kb on - strand, within gspF at 3458.913 kb on + strand, within gspF at 3458.920 kb on + strand at 3458.928 kb on - strand at 3458.936 kb on + strand at 3458.938 kb on - strand at 3458.949 kb on - strand at 3458.978 kb on + strand at 3458.980 kb on + strand at 3458.986 kb on - strand at 3459.001 kb on + strand at 3459.004 kb on - strand at 3459.015 kb on - strand at 3459.027 kb on - strand at 3459.078 kb on - strand at 3459.078 kb on - strand at 3459.203 kb on - strand, within gspG at 3459.203 kb on - strand, within gspG at 3459.226 kb on - strand, within gspG at 3459.244 kb on - strand, within gspG at 3459.252 kb on + strand, within gspG at 3459.252 kb on + strand, within gspG at 3459.288 kb on - strand, within gspG at 3459.288 kb on - strand, within gspG at 3459.320 kb on + strand, within gspG at 3459.320 kb on + strand, within gspG at 3459.324 kb on + strand, within gspG at 3459.355 kb on - strand, within gspG at 3459.360 kb on + strand, within gspG at 3459.360 kb on + strand, within gspG at 3459.360 kb on + strand, within gspG at 3459.360 kb on + strand, within gspG at 3459.368 kb on - strand, within gspG at 3459.371 kb on + strand, within gspG at 3459.389 kb on - strand, within gspG at 3459.391 kb on + strand, within gspG at 3459.391 kb on + strand, within gspG at 3459.391 kb on + strand, within gspG at 3459.399 kb on - strand, within gspG at 3459.421 kb on + strand, within gspG at 3459.446 kb on - strand at 3459.460 kb on - strand at 3459.460 kb on - strand at 3459.519 kb on - strand at 3459.519 kb on - strand at 3459.530 kb on - strand at 3459.530 kb on - strand at 3459.540 kb on - strand at 3459.570 kb on + strand, within gspH at 3459.578 kb on - strand, within gspH at 3459.603 kb on - strand, within gspH at 3459.607 kb on - strand, within gspH at 3459.607 kb on - strand, within gspH at 3459.674 kb on - strand, within gspH at 3459.698 kb on - strand, within gspH at 3459.716 kb on + strand, within gspH at 3459.742 kb on + strand, within gspH at 3459.742 kb on + strand, within gspH at 3459.747 kb on + strand, within gspH at 3459.818 kb on + strand, within gspH at 3459.851 kb on - strand, within gspH at 3459.851 kb on - strand, within gspH at 3459.893 kb on + strand, within gspH at 3459.920 kb on - strand, within gspH at 3459.930 kb on + strand, within gspH at 3459.937 kb on + strand, within gspH at 3459.937 kb on + strand, within gspH at 3459.947 kb on + strand, within gspH at 3459.947 kb on + strand, within gspH at 3459.955 kb on - strand at 3459.964 kb on - strand at 3459.978 kb on + strand at 3459.978 kb on + strand at 3459.978 kb on + strand at 3459.995 kb on + strand at 3460.012 kb on + strand at 3460.012 kb on + strand at 3460.031 kb on - strand at 3460.043 kb on - strand, within yheH at 3460.057 kb on + strand, within yheH at 3460.081 kb on + strand, within yheH at 3460.081 kb on + strand, within yheH at 3460.081 kb on + strand, within yheH at 3460.081 kb on + strand, within yheH at 3460.083 kb on + strand, within yheH at 3460.083 kb on + strand, within yheH at 3460.086 kb on - strand, within yheH at 3460.086 kb on - strand, within yheH at 3460.086 kb on - strand, within yheH at 3460.089 kb on - strand, within yheH at 3460.089 kb on - strand, within yheH at 3460.107 kb on + strand, within yheH at 3460.114 kb on + strand, within yheH at 3460.133 kb on + strand, within yheH at 3460.198 kb on - strand, within yheH at 3460.198 kb on - strand, within yheH at 3460.224 kb on + strand, within yheH at 3460.248 kb on - strand, within yheH at 3460.248 kb on - strand, within yheH at 3460.248 kb on - strand, within yheH at 3460.277 kb on - strand, within yheH at 3460.280 kb on + strand, within yheH at 3460.280 kb on + strand, within yheH at 3460.280 kb on + strand, within yheH at 3460.280 kb on + strand, within yheH at 3460.280 kb on + strand, within yheH at 3460.385 kb on + strand at 3460.388 kb on - strand at 3460.395 kb on + strand at 3460.395 kb on + strand at 3460.401 kb on + strand at 3460.439 kb on - strand, within gspJ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 3,458,048 + gspF b3327 0.17 -1.0 3,458,067 + gspF b3327 0.19 -1.4 3,458,067 + gspF b3327 0.19 -2.4 3,458,074 - gspF b3327 0.20 +1.0 3,458,085 + gspF b3327 0.21 -0.7 3,458,093 - gspF b3327 0.21 -0.4 3,458,113 - gspF b3327 0.23 +0.1 3,458,141 + gspF b3327 0.25 -0.1 3,458,218 + gspF b3327 0.32 +0.5 3,458,223 - gspF b3327 0.32 +0.9 3,458,223 - gspF b3327 0.32 +0.1 3,458,223 - gspF b3327 0.32 +0.1 3,458,223 - gspF b3327 0.32 +0.2 3,458,223 - gspF b3327 0.32 -0.3 3,458,223 - gspF b3327 0.32 -0.8 3,458,243 - gspF b3327 0.34 -0.1 3,458,243 - gspF b3327 0.34 -1.7 3,458,324 + gspF b3327 0.41 +0.4 3,458,324 + gspF b3327 0.41 -1.0 3,458,324 + gspF b3327 0.41 +0.9 3,458,338 - gspF b3327 0.42 -0.9 3,458,338 - gspF b3327 0.42 +1.1 3,458,348 + gspF b3327 0.43 +1.6 3,458,348 + gspF b3327 0.43 -1.5 3,458,353 - gspF b3327 0.43 +0.2 3,458,358 - gspF b3327 0.43 -0.6 3,458,406 + gspF b3327 0.47 +0.2 3,458,420 + gspF b3327 0.49 -0.5 3,458,420 + gspF b3327 0.49 +0.1 3,458,428 - gspF b3327 0.49 +0.1 3,458,453 + gspF b3327 0.51 -0.7 3,458,475 + gspF b3327 0.53 +0.1 3,458,505 - gspF b3327 0.56 +0.2 3,458,521 - gspF b3327 0.57 +0.5 3,458,521 - gspF b3327 0.57 -0.4 3,458,632 - gspF b3327 0.66 -0.5 3,458,632 - gspF b3327 0.66 -0.4 3,458,638 + gspF b3327 0.67 +0.7 3,458,663 - gspF b3327 0.69 -0.2 3,458,668 + gspF b3327 0.69 -0.2 3,458,671 - gspF b3327 0.70 +0.6 3,458,687 - gspF b3327 0.71 +0.4 3,458,687 - gspF b3327 0.71 +0.9 3,458,689 + gspF b3327 0.71 -1.5 3,458,711 + gspF b3327 0.73 +0.5 3,458,817 + gspF b3327 0.82 +0.2 3,458,817 + gspF b3327 0.82 +0.5 3,458,822 + gspF b3327 0.82 +1.4 3,458,822 + gspF b3327 0.82 -1.0 3,458,823 + gspF b3327 0.82 +0.2 3,458,878 + gspF b3327 0.87 -1.5 3,458,889 + gspF b3327 0.88 -0.2 3,458,899 - gspF b3327 0.89 +0.6 3,458,909 - gspF b3327 0.89 +0.2 3,458,913 + gspF b3327 0.90 +0.6 3,458,920 + +0.7 3,458,928 - -0.6 3,458,936 + -2.1 3,458,938 - -0.2 3,458,949 - -1.0 3,458,978 + +0.3 3,458,980 + +0.3 3,458,986 - +0.5 3,459,001 + +0.4 3,459,004 - -0.5 3,459,015 - -0.5 3,459,027 - +0.3 3,459,078 - +0.3 3,459,078 - +0.8 3,459,203 - gspG b3328 0.36 +0.6 3,459,203 - gspG b3328 0.36 -0.2 3,459,226 - gspG b3328 0.41 -0.8 3,459,244 - gspG b3328 0.45 +0.1 3,459,252 + gspG b3328 0.47 -2.6 3,459,252 + gspG b3328 0.47 -0.0 3,459,288 - gspG b3328 0.55 -0.0 3,459,288 - gspG b3328 0.55 -1.0 3,459,320 + gspG b3328 0.63 -1.1 3,459,320 + gspG b3328 0.63 -0.8 3,459,324 + gspG b3328 0.64 -0.8 3,459,355 - gspG b3328 0.71 +0.5 3,459,360 + gspG b3328 0.72 -0.0 3,459,360 + gspG b3328 0.72 -0.9 3,459,360 + gspG b3328 0.72 -0.8 3,459,360 + gspG b3328 0.72 -1.5 3,459,368 - gspG b3328 0.74 -0.8 3,459,371 + gspG b3328 0.74 -0.2 3,459,389 - gspG b3328 0.79 -0.7 3,459,391 + gspG b3328 0.79 +0.4 3,459,391 + gspG b3328 0.79 +0.4 3,459,391 + gspG b3328 0.79 +0.4 3,459,399 - gspG b3328 0.81 -1.6 3,459,421 + gspG b3328 0.86 -1.5 3,459,446 - -1.9 3,459,460 - +1.3 3,459,460 - +0.6 3,459,519 - -2.0 3,459,519 - +0.4 3,459,530 - -1.7 3,459,530 - -1.2 3,459,540 - -0.2 3,459,570 + gspH b3329 0.16 +0.8 3,459,578 - gspH b3329 0.17 +1.1 3,459,603 - gspH b3329 0.22 -0.7 3,459,607 - gspH b3329 0.23 -1.4 3,459,607 - gspH b3329 0.23 +0.2 3,459,674 - gspH b3329 0.36 +1.5 3,459,698 - gspH b3329 0.41 -0.7 3,459,716 + gspH b3329 0.44 +0.3 3,459,742 + gspH b3329 0.49 -0.2 3,459,742 + gspH b3329 0.49 +0.7 3,459,747 + gspH b3329 0.50 +1.0 3,459,818 + gspH b3329 0.64 -1.7 3,459,851 - gspH b3329 0.71 -0.8 3,459,851 - gspH b3329 0.71 -0.6 3,459,893 + gspH b3329 0.79 -0.2 3,459,920 - gspH b3329 0.84 +0.3 3,459,930 + gspH b3329 0.86 -0.8 3,459,937 + gspH b3329 0.88 -0.7 3,459,937 + gspH b3329 0.88 -0.0 3,459,947 + gspH b3329 0.90 -1.0 3,459,947 + gspH b3329 0.90 +0.7 3,459,955 - +0.8 3,459,964 - +0.6 3,459,978 + +0.9 3,459,978 + -0.0 3,459,978 + -0.3 3,459,995 + -3.4 3,460,012 + -1.4 3,460,012 + -0.9 3,460,031 - +0.5 3,460,043 - yheH b3330 0.12 -0.1 3,460,057 + yheH b3330 0.16 +2.5 3,460,081 + yheH b3330 0.22 -0.7 3,460,081 + yheH b3330 0.22 -1.7 3,460,081 + yheH b3330 0.22 +0.4 3,460,081 + yheH b3330 0.22 +1.0 3,460,083 + yheH b3330 0.23 -1.2 3,460,083 + yheH b3330 0.23 +0.8 3,460,086 - yheH b3330 0.24 +0.6 3,460,086 - yheH b3330 0.24 -0.3 3,460,086 - yheH b3330 0.24 -0.5 3,460,089 - yheH b3330 0.25 -0.9 3,460,089 - yheH b3330 0.25 +1.0 3,460,107 + yheH b3330 0.29 +0.4 3,460,114 + yheH b3330 0.31 -1.3 3,460,133 + yheH b3330 0.36 -0.3 3,460,198 - yheH b3330 0.53 -0.1 3,460,198 - yheH b3330 0.53 -0.1 3,460,224 + yheH b3330 0.60 +0.3 3,460,248 - yheH b3330 0.67 -1.0 3,460,248 - yheH b3330 0.67 -0.3 3,460,248 - yheH b3330 0.67 -2.1 3,460,277 - yheH b3330 0.74 +0.1 3,460,280 + yheH b3330 0.75 -0.9 3,460,280 + yheH b3330 0.75 -0.1 3,460,280 + yheH b3330 0.75 +0.5 3,460,280 + yheH b3330 0.75 -0.9 3,460,280 + yheH b3330 0.75 -0.8 3,460,385 + +0.5 3,460,388 - -0.4 3,460,395 + +1.2 3,460,395 + +0.7 3,460,401 + +0.1 3,460,439 - gspJ b3331 0.12 +0.3
Or see this region's nucleotide sequence