Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rlmE and yhbY are separated by 125 nucleotides yhbY and greA are separated by 155 nucleotides greA and dacB are separated by 247 nucleotides
b3179: rlmE - 23S rRNA U2552 ribose 2'-O-methyltransferase, SAM-dependent (NCBI), at 3,325,057 to 3,325,686
rlmE
b3180: yhbY - predicted RNA-binding protein (NCBI), at 3,325,812 to 3,326,105
yhbY
b3181: greA - transcription elongation factor: cleaves 3' nucleotide of paused mRNA (VIMSS), at 3,326,261 to 3,326,737
greA
b3182: dacB - D-alanyl-D-alanine carboxypeptidase (NCBI), at 3,326,985 to 3,328,418
dacB
Position (kb)
3326
3327 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3325.749 kb on - strand at 3325.849 kb on + strand, within yhbY at 3325.849 kb on + strand at 3325.883 kb on - strand, within yhbY at 3325.908 kb on - strand, within yhbY at 3325.913 kb on + strand, within yhbY at 3325.921 kb on - strand, within yhbY at 3325.928 kb on + strand, within yhbY at 3325.932 kb on + strand, within yhbY at 3325.939 kb on + strand at 3325.940 kb on - strand, within yhbY at 3325.940 kb on - strand, within yhbY at 3325.976 kb on - strand, within yhbY at 3326.010 kb on - strand, within yhbY at 3326.015 kb on - strand, within yhbY at 3326.015 kb on - strand, within yhbY at 3326.066 kb on - strand, within yhbY at 3326.187 kb on - strand at 3326.190 kb on - strand at 3326.197 kb on + strand at 3326.267 kb on - strand at 3326.278 kb on - strand at 3326.278 kb on - strand at 3326.323 kb on + strand, within greA at 3326.338 kb on - strand, within greA at 3326.500 kb on - strand, within greA at 3326.500 kb on - strand, within greA at 3326.548 kb on + strand, within greA at 3326.548 kb on + strand, within greA at 3326.552 kb on + strand, within greA at 3326.552 kb on + strand, within greA at 3326.602 kb on - strand, within greA at 3326.653 kb on + strand, within greA at 3326.711 kb on + strand at 3326.711 kb on + strand at 3326.719 kb on - strand at 3326.719 kb on - strand at 3326.730 kb on - strand at 3326.738 kb on + strand at 3326.746 kb on - strand at 3326.746 kb on - strand at 3326.772 kb on + strand at 3326.788 kb on + strand at 3326.810 kb on + strand at 3326.810 kb on + strand at 3326.867 kb on + strand at 3326.875 kb on - strand at 3326.899 kb on - strand at 3326.901 kb on + strand at 3326.943 kb on + strand at 3326.980 kb on + strand at 3327.019 kb on + strand at 3327.027 kb on - strand at 3327.030 kb on - strand at 3327.034 kb on + strand at 3327.059 kb on + strand at 3327.064 kb on + strand at 3327.066 kb on + strand at 3327.069 kb on - strand at 3327.088 kb on + strand at 3327.132 kb on + strand, within dacB at 3327.161 kb on + strand, within dacB at 3327.161 kb on + strand, within dacB at 3327.413 kb on - strand, within dacB at 3327.434 kb on - strand, within dacB at 3327.494 kb on - strand, within dacB at 3327.494 kb on - strand, within dacB at 3327.515 kb on + strand, within dacB at 3327.523 kb on - strand, within dacB at 3327.533 kb on - strand, within dacB at 3327.533 kb on - strand, within dacB at 3327.574 kb on - strand, within dacB at 3327.574 kb on - strand, within dacB at 3327.584 kb on + strand, within dacB at 3327.597 kb on - strand, within dacB at 3327.632 kb on - strand, within dacB at 3327.649 kb on - strand, within dacB at 3327.649 kb on - strand, within dacB at 3327.656 kb on - strand, within dacB at 3327.656 kb on - strand, within dacB at 3327.697 kb on + strand, within dacB at 3327.697 kb on + strand, within dacB at 3327.731 kb on + strand, within dacB at 3327.731 kb on + strand, within dacB at 3327.734 kb on - strand, within dacB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 3,325,749 - -1.8 3,325,849 + yhbY b3180 0.13 -0.4 3,325,849 + +0.3 3,325,883 - yhbY b3180 0.24 -1.0 3,325,908 - yhbY b3180 0.33 -2.2 3,325,913 + yhbY b3180 0.34 +0.4 3,325,921 - yhbY b3180 0.37 -0.8 3,325,928 + yhbY b3180 0.39 -0.4 3,325,932 + yhbY b3180 0.41 +0.9 3,325,939 + +1.9 3,325,940 - yhbY b3180 0.44 -0.6 3,325,940 - yhbY b3180 0.44 -0.7 3,325,976 - yhbY b3180 0.56 -0.7 3,326,010 - yhbY b3180 0.67 +0.3 3,326,015 - yhbY b3180 0.69 -0.2 3,326,015 - yhbY b3180 0.69 +1.4 3,326,066 - yhbY b3180 0.86 -1.0 3,326,187 - +0.6 3,326,190 - +0.9 3,326,197 + -0.6 3,326,267 - -0.5 3,326,278 - -1.1 3,326,278 - +1.2 3,326,323 + greA b3181 0.13 -0.1 3,326,338 - greA b3181 0.16 +1.1 3,326,500 - greA b3181 0.50 +1.4 3,326,500 - greA b3181 0.50 +2.5 3,326,548 + greA b3181 0.60 +0.1 3,326,548 + greA b3181 0.60 +1.9 3,326,552 + greA b3181 0.61 -0.6 3,326,552 + greA b3181 0.61 +0.3 3,326,602 - greA b3181 0.71 +0.2 3,326,653 + greA b3181 0.82 +1.6 3,326,711 + +1.4 3,326,711 + +0.8 3,326,719 - +1.2 3,326,719 - +1.7 3,326,730 - +1.5 3,326,738 + +2.2 3,326,746 - +1.3 3,326,746 - +0.9 3,326,772 + -0.4 3,326,788 + +0.4 3,326,810 + -0.7 3,326,810 + +0.9 3,326,867 + +0.8 3,326,875 - -0.4 3,326,899 - -0.3 3,326,901 + -1.7 3,326,943 + +0.2 3,326,980 + +0.6 3,327,019 + +0.3 3,327,027 - +0.8 3,327,030 - +0.4 3,327,034 + +1.3 3,327,059 + -0.7 3,327,064 + +1.0 3,327,066 + +0.2 3,327,069 - +0.8 3,327,088 + -0.0 3,327,132 + dacB b3182 0.10 +0.9 3,327,161 + dacB b3182 0.12 -1.6 3,327,161 + dacB b3182 0.12 +0.0 3,327,413 - dacB b3182 0.30 -0.7 3,327,434 - dacB b3182 0.31 -0.1 3,327,494 - dacB b3182 0.35 -1.5 3,327,494 - dacB b3182 0.35 +1.4 3,327,515 + dacB b3182 0.37 -0.9 3,327,523 - dacB b3182 0.38 +0.2 3,327,533 - dacB b3182 0.38 -1.1 3,327,533 - dacB b3182 0.38 -1.1 3,327,574 - dacB b3182 0.41 -0.4 3,327,574 - dacB b3182 0.41 -2.2 3,327,584 + dacB b3182 0.42 +0.4 3,327,597 - dacB b3182 0.43 +1.0 3,327,632 - dacB b3182 0.45 +0.9 3,327,649 - dacB b3182 0.46 +0.2 3,327,649 - dacB b3182 0.46 -0.1 3,327,656 - dacB b3182 0.47 +0.0 3,327,656 - dacB b3182 0.47 +0.2 3,327,697 + dacB b3182 0.50 +0.6 3,327,697 + dacB b3182 0.50 +0.1 3,327,731 + dacB b3182 0.52 -1.2 3,327,731 + dacB b3182 0.52 -0.1 3,327,734 - dacB b3182 0.52 -2.4
Or see this region's nucleotide sequence