Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yraO and yraP are separated by 9 nucleotides yraP and yraQ are separated by 113 nucleotides yraQ and yraR are separated by 72 nucleotides yraR and yhbO are separated by 127 nucleotides
b3149: yraO - DnaA initiator-associating factor for replication initiation (NCBI), at 3,293,831 to 3,294,421
yraO
b3150: yraP - hypothetical protein (NCBI), at 3,294,431 to 3,295,006
yraP
b3151: yraQ - predicted permease (NCBI), at 3,295,120 to 3,296,160
yraQ
b3152: yraR - orf, hypothetical protein (VIMSS), at 3,296,233 to 3,296,868
yraR
b3153: yhbO - orf, hypothetical protein (VIMSS), at 3,296,996 to 3,297,514
yhbO
Position (kb)
3295
3296
3297 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3294.136 kb on + strand, within yraO at 3294.157 kb on - strand, within yraO at 3294.260 kb on + strand, within yraO at 3294.286 kb on - strand, within yraO at 3294.286 kb on - strand, within yraO at 3294.309 kb on - strand, within yraO at 3294.318 kb on - strand, within yraO at 3294.353 kb on + strand, within yraO at 3294.353 kb on + strand, within yraO at 3294.361 kb on + strand, within yraO at 3294.361 kb on + strand, within yraO at 3294.365 kb on + strand at 3294.376 kb on + strand at 3294.376 kb on + strand at 3294.391 kb on - strand at 3294.391 kb on - strand at 3294.415 kb on - strand at 3294.429 kb on + strand at 3294.465 kb on - strand at 3294.481 kb on + strand at 3294.503 kb on - strand, within yraP at 3294.503 kb on - strand, within yraP at 3294.510 kb on + strand, within yraP at 3294.563 kb on - strand, within yraP at 3294.563 kb on - strand, within yraP at 3294.612 kb on - strand, within yraP at 3294.612 kb on - strand, within yraP at 3294.619 kb on + strand, within yraP at 3294.680 kb on - strand, within yraP at 3294.716 kb on - strand, within yraP at 3294.728 kb on + strand, within yraP at 3294.728 kb on + strand, within yraP at 3294.729 kb on + strand, within yraP at 3294.736 kb on - strand, within yraP at 3294.736 kb on - strand, within yraP at 3294.751 kb on + strand, within yraP at 3294.751 kb on + strand, within yraP at 3294.787 kb on + strand, within yraP at 3294.790 kb on - strand, within yraP at 3294.818 kb on + strand, within yraP at 3294.966 kb on + strand at 3294.966 kb on + strand at 3294.966 kb on + strand at 3295.019 kb on - strand at 3295.019 kb on - strand at 3295.077 kb on - strand at 3295.079 kb on + strand at 3295.079 kb on + strand at 3295.095 kb on - strand at 3295.159 kb on - strand at 3295.159 kb on - strand at 3295.160 kb on - strand at 3295.222 kb on - strand at 3295.223 kb on - strand at 3295.249 kb on - strand, within yraQ at 3295.288 kb on + strand, within yraQ at 3295.350 kb on - strand, within yraQ at 3295.350 kb on - strand, within yraQ at 3295.384 kb on + strand, within yraQ at 3295.392 kb on - strand, within yraQ at 3295.394 kb on + strand, within yraQ at 3295.443 kb on + strand, within yraQ at 3295.443 kb on + strand, within yraQ at 3295.451 kb on - strand, within yraQ at 3295.461 kb on - strand, within yraQ at 3295.559 kb on - strand, within yraQ at 3295.559 kb on - strand, within yraQ at 3295.618 kb on - strand, within yraQ at 3295.633 kb on - strand, within yraQ at 3295.690 kb on + strand, within yraQ at 3295.791 kb on - strand, within yraQ at 3295.791 kb on - strand, within yraQ at 3295.824 kb on - strand, within yraQ at 3295.850 kb on + strand, within yraQ at 3295.911 kb on - strand, within yraQ at 3295.915 kb on - strand, within yraQ at 3296.007 kb on + strand, within yraQ at 3296.053 kb on - strand, within yraQ at 3296.053 kb on - strand, within yraQ at 3296.059 kb on - strand at 3296.059 kb on - strand at 3296.196 kb on + strand at 3296.201 kb on + strand at 3296.201 kb on + strand at 3296.221 kb on - strand at 3296.221 kb on - strand at 3296.289 kb on - strand at 3296.289 kb on - strand at 3296.294 kb on - strand at 3296.441 kb on - strand, within yraR at 3296.441 kb on - strand, within yraR at 3296.466 kb on - strand, within yraR at 3296.549 kb on + strand, within yraR at 3296.661 kb on + strand, within yraR at 3296.661 kb on + strand, within yraR at 3296.687 kb on - strand, within yraR at 3296.737 kb on - strand, within yraR at 3296.737 kb on - strand, within yraR at 3296.819 kb on + strand at 3296.819 kb on + strand at 3296.830 kb on + strand at 3296.927 kb on - strand at 3296.992 kb on + strand at 3296.992 kb on + strand at 3297.032 kb on - strand at 3297.149 kb on - strand, within yhbO at 3297.149 kb on - strand, within yhbO
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 3,294,136 + yraO b3149 0.52 +0.9 3,294,157 - yraO b3149 0.55 -2.0 3,294,260 + yraO b3149 0.73 -0.6 3,294,286 - yraO b3149 0.77 +1.9 3,294,286 - yraO b3149 0.77 +1.5 3,294,309 - yraO b3149 0.81 +0.0 3,294,318 - yraO b3149 0.82 -1.0 3,294,353 + yraO b3149 0.88 -0.8 3,294,353 + yraO b3149 0.88 -1.8 3,294,361 + yraO b3149 0.90 -2.7 3,294,361 + yraO b3149 0.90 -0.0 3,294,365 + -0.1 3,294,376 + -2.5 3,294,376 + -0.2 3,294,391 - -2.0 3,294,391 - -0.2 3,294,415 - +1.1 3,294,429 + -0.4 3,294,465 - +0.8 3,294,481 + -0.4 3,294,503 - yraP b3150 0.12 -0.0 3,294,503 - yraP b3150 0.12 -0.6 3,294,510 + yraP b3150 0.14 +0.9 3,294,563 - yraP b3150 0.23 -0.6 3,294,563 - yraP b3150 0.23 -0.4 3,294,612 - yraP b3150 0.31 -1.7 3,294,612 - yraP b3150 0.31 +0.3 3,294,619 + yraP b3150 0.33 -0.1 3,294,680 - yraP b3150 0.43 -1.9 3,294,716 - yraP b3150 0.49 +0.3 3,294,728 + yraP b3150 0.52 -1.3 3,294,728 + yraP b3150 0.52 +0.4 3,294,729 + yraP b3150 0.52 -0.6 3,294,736 - yraP b3150 0.53 -1.1 3,294,736 - yraP b3150 0.53 -1.2 3,294,751 + yraP b3150 0.56 +0.5 3,294,751 + yraP b3150 0.56 -0.5 3,294,787 + yraP b3150 0.62 +1.0 3,294,790 - yraP b3150 0.62 -1.1 3,294,818 + yraP b3150 0.67 -1.3 3,294,966 + -0.4 3,294,966 + +0.7 3,294,966 + -1.1 3,295,019 - +0.3 3,295,019 - +1.2 3,295,077 - +1.4 3,295,079 + +4.4 3,295,079 + -0.3 3,295,095 - -0.1 3,295,159 - +0.1 3,295,159 - +1.2 3,295,160 - +0.2 3,295,222 - +0.4 3,295,223 - +0.5 3,295,249 - yraQ b3151 0.12 +0.8 3,295,288 + yraQ b3151 0.16 -0.0 3,295,350 - yraQ b3151 0.22 -0.9 3,295,350 - yraQ b3151 0.22 +2.8 3,295,384 + yraQ b3151 0.25 -1.3 3,295,392 - yraQ b3151 0.26 -0.1 3,295,394 + yraQ b3151 0.26 +0.9 3,295,443 + yraQ b3151 0.31 -0.8 3,295,443 + yraQ b3151 0.31 +0.2 3,295,451 - yraQ b3151 0.32 +0.8 3,295,461 - yraQ b3151 0.33 +1.7 3,295,559 - yraQ b3151 0.42 -0.6 3,295,559 - yraQ b3151 0.42 +0.4 3,295,618 - yraQ b3151 0.48 +0.8 3,295,633 - yraQ b3151 0.49 +0.4 3,295,690 + yraQ b3151 0.55 -0.1 3,295,791 - yraQ b3151 0.64 +0.6 3,295,791 - yraQ b3151 0.64 +1.1 3,295,824 - yraQ b3151 0.68 +0.9 3,295,850 + yraQ b3151 0.70 +0.8 3,295,911 - yraQ b3151 0.76 +0.6 3,295,915 - yraQ b3151 0.76 +0.5 3,296,007 + yraQ b3151 0.85 +0.0 3,296,053 - yraQ b3151 0.90 +0.5 3,296,053 - yraQ b3151 0.90 +0.8 3,296,059 - -1.7 3,296,059 - +0.3 3,296,196 + -0.1 3,296,201 + -1.7 3,296,201 + +0.3 3,296,221 - -1.9 3,296,221 - -2.2 3,296,289 - +0.7 3,296,289 - -0.2 3,296,294 - +0.1 3,296,441 - yraR b3152 0.33 +0.2 3,296,441 - yraR b3152 0.33 +0.1 3,296,466 - yraR b3152 0.37 -1.9 3,296,549 + yraR b3152 0.50 -0.1 3,296,661 + yraR b3152 0.67 +1.7 3,296,661 + yraR b3152 0.67 +0.3 3,296,687 - yraR b3152 0.71 -0.2 3,296,737 - yraR b3152 0.79 +0.9 3,296,737 - yraR b3152 0.79 -0.5 3,296,819 + -0.1 3,296,819 + +0.1 3,296,830 + -0.0 3,296,927 - -1.3 3,296,992 + -0.0 3,296,992 + -0.4 3,297,032 - +0.2 3,297,149 - yhbO b3153 0.29 -0.3 3,297,149 - yhbO b3153 0.29 +1.7
Or see this region's nucleotide sequence