Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cca and bacA are separated by 180 nucleotides bacA and ygiG are separated by 89 nucleotides ygiG and ygiH are separated by 104 nucleotides
b3056: cca - fused tRNA nucleotidyl transferase/2'3'-cyclic phosphodiesterase/2'nucleotidase and phosphatase (NCBI), at 3,199,913 to 3,201,151
cca
b3057: bacA - undecaprenyl pyrophosphate phosphatase (NCBI), at 3,201,332 to 3,202,153
bacA
b3058: ygiG - putative kinase (VIMSS), at 3,202,243 to 3,202,611
ygiG
b3059: ygiH - hypothetical protein (NCBI), at 3,202,716 to 3,203,333
ygiH
Position (kb)
3201
3202
3203 Strain fitness (log2 ratio)
-2
-1
0
1 at 3201.212 kb on + strand at 3201.294 kb on + strand at 3201.343 kb on - strand at 3201.352 kb on - strand at 3201.439 kb on + strand, within bacA at 3201.489 kb on - strand, within bacA at 3201.489 kb on - strand, within bacA at 3201.512 kb on + strand, within bacA at 3201.512 kb on + strand, within bacA at 3201.537 kb on - strand, within bacA at 3201.537 kb on - strand, within bacA at 3201.640 kb on + strand, within bacA at 3201.640 kb on + strand, within bacA at 3201.696 kb on + strand, within bacA at 3201.707 kb on - strand, within bacA at 3201.707 kb on - strand, within bacA at 3201.765 kb on - strand, within bacA at 3201.880 kb on + strand, within bacA at 3201.970 kb on - strand, within bacA at 3201.978 kb on - strand, within bacA at 3201.995 kb on - strand, within bacA at 3202.065 kb on - strand, within bacA at 3202.069 kb on - strand, within bacA at 3202.069 kb on - strand, within bacA at 3202.081 kb on - strand at 3202.081 kb on - strand at 3202.136 kb on + strand at 3202.136 kb on + strand at 3202.139 kb on + strand at 3202.187 kb on + strand at 3202.219 kb on - strand at 3202.602 kb on - strand at 3202.644 kb on - strand at 3202.712 kb on + strand at 3202.878 kb on + strand, within ygiH at 3202.878 kb on + strand, within ygiH at 3202.968 kb on - strand, within ygiH at 3203.057 kb on + strand, within ygiH at 3203.072 kb on - strand, within ygiH at 3203.128 kb on - strand, within ygiH at 3203.143 kb on + strand, within ygiH
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 3,201,212 + -1.1 3,201,294 + -0.6 3,201,343 - +0.2 3,201,352 - +1.3 3,201,439 + bacA b3057 0.13 +0.7 3,201,489 - bacA b3057 0.19 +0.4 3,201,489 - bacA b3057 0.19 +1.6 3,201,512 + bacA b3057 0.22 -0.4 3,201,512 + bacA b3057 0.22 +1.7 3,201,537 - bacA b3057 0.25 +0.7 3,201,537 - bacA b3057 0.25 -1.4 3,201,640 + bacA b3057 0.37 -0.4 3,201,640 + bacA b3057 0.37 +0.9 3,201,696 + bacA b3057 0.44 +0.7 3,201,707 - bacA b3057 0.46 -0.1 3,201,707 - bacA b3057 0.46 +0.5 3,201,765 - bacA b3057 0.53 +1.2 3,201,880 + bacA b3057 0.67 +0.7 3,201,970 - bacA b3057 0.78 -0.9 3,201,978 - bacA b3057 0.79 +0.9 3,201,995 - bacA b3057 0.81 +0.6 3,202,065 - bacA b3057 0.89 +1.3 3,202,069 - bacA b3057 0.90 -1.1 3,202,069 - bacA b3057 0.90 -0.6 3,202,081 - +1.4 3,202,081 - +1.4 3,202,136 + +0.3 3,202,136 + -2.3 3,202,139 + +0.7 3,202,187 + +0.9 3,202,219 - +1.2 3,202,602 - -0.6 3,202,644 - -0.4 3,202,712 + +0.1 3,202,878 + ygiH b3059 0.26 +0.5 3,202,878 + ygiH b3059 0.26 -0.8 3,202,968 - ygiH b3059 0.41 -0.5 3,203,057 + ygiH b3059 0.55 -0.1 3,203,072 - ygiH b3059 0.58 -1.4 3,203,128 - ygiH b3059 0.67 -1.2 3,203,143 + ygiH b3059 0.69 +0.4
Or see this region's nucleotide sequence