Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yghA and exbD are separated by 271 nucleotides exbD and exbB are separated by 6 nucleotides exbB and metC are separated by 251 nucleotides
b3003: yghA - oxidoreductase (NCBI), at 3,147,684 to 3,148,568
yghA
b3005: exbD - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,148,840 to 3,149,265
exbD
b3006: exbB - membrane spanning protein in TonB-ExbB-ExbD complex (NCBI), at 3,149,272 to 3,150,006
exbB
b3008: metC - cystathionine beta-lyase (NCBI), at 3,150,258 to 3,151,445
metC
Position (kb)
3149
3150
3151 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3148.282 kb on - strand, within yghA at 3148.282 kb on - strand, within yghA at 3148.299 kb on + strand, within yghA at 3148.299 kb on + strand, within yghA at 3148.371 kb on + strand, within yghA at 3148.384 kb on - strand, within yghA at 3148.384 kb on - strand, within yghA at 3148.386 kb on + strand, within yghA at 3148.386 kb on + strand, within yghA at 3148.387 kb on - strand, within yghA at 3148.387 kb on - strand, within yghA at 3148.515 kb on + strand at 3148.519 kb on + strand at 3148.540 kb on - strand at 3148.552 kb on - strand at 3148.611 kb on - strand at 3148.694 kb on + strand at 3148.700 kb on - strand at 3148.758 kb on - strand at 3148.758 kb on - strand at 3148.875 kb on - strand at 3148.906 kb on + strand, within exbD at 3148.936 kb on - strand, within exbD at 3148.992 kb on - strand, within exbD at 3149.036 kb on + strand, within exbD at 3149.092 kb on + strand, within exbD at 3149.120 kb on - strand at 3149.120 kb on - strand, within exbD at 3149.131 kb on + strand, within exbD at 3149.131 kb on + strand, within exbD at 3149.208 kb on + strand, within exbD at 3149.377 kb on + strand, within exbB at 3149.377 kb on + strand, within exbB at 3149.380 kb on + strand, within exbB at 3149.396 kb on - strand, within exbB at 3149.448 kb on - strand, within exbB at 3149.501 kb on + strand, within exbB at 3149.508 kb on + strand, within exbB at 3149.543 kb on - strand, within exbB at 3149.553 kb on - strand, within exbB at 3149.554 kb on + strand, within exbB at 3149.564 kb on + strand, within exbB at 3149.659 kb on + strand, within exbB at 3149.682 kb on - strand, within exbB at 3149.682 kb on - strand, within exbB at 3149.690 kb on - strand, within exbB at 3149.707 kb on + strand, within exbB at 3149.710 kb on - strand, within exbB at 3149.718 kb on + strand, within exbB at 3149.803 kb on - strand, within exbB at 3149.803 kb on - strand, within exbB at 3149.866 kb on + strand, within exbB at 3149.956 kb on + strand at 3149.956 kb on + strand at 3149.956 kb on + strand at 3149.983 kb on + strand at 3150.016 kb on - strand at 3150.257 kb on - strand at 3150.288 kb on - strand at 3150.292 kb on + strand at 3150.300 kb on - strand at 3150.300 kb on - strand at 3150.337 kb on - strand at 3150.378 kb on - strand, within metC at 3150.421 kb on + strand, within metC at 3150.421 kb on + strand, within metC at 3150.514 kb on - strand, within metC at 3150.575 kb on + strand, within metC at 3150.577 kb on - strand, within metC at 3150.583 kb on - strand, within metC at 3150.640 kb on - strand, within metC at 3150.654 kb on + strand, within metC at 3150.669 kb on + strand, within metC at 3150.706 kb on - strand, within metC at 3150.791 kb on - strand, within metC at 3150.820 kb on + strand, within metC at 3150.820 kb on + strand, within metC at 3150.912 kb on + strand, within metC at 3150.914 kb on + strand, within metC at 3150.915 kb on + strand, within metC at 3150.932 kb on - strand, within metC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 3,148,282 - yghA b3003 0.68 -0.5 3,148,282 - yghA b3003 0.68 +0.1 3,148,299 + yghA b3003 0.69 -0.0 3,148,299 + yghA b3003 0.69 -1.1 3,148,371 + yghA b3003 0.78 -0.4 3,148,384 - yghA b3003 0.79 +1.0 3,148,384 - yghA b3003 0.79 -2.8 3,148,386 + yghA b3003 0.79 -0.6 3,148,386 + yghA b3003 0.79 -0.2 3,148,387 - yghA b3003 0.79 -1.1 3,148,387 - yghA b3003 0.79 -0.4 3,148,515 + +0.4 3,148,519 + -1.1 3,148,540 - +0.1 3,148,552 - +0.9 3,148,611 - +1.2 3,148,694 + -0.1 3,148,700 - -0.7 3,148,758 - -1.1 3,148,758 - -1.3 3,148,875 - +1.2 3,148,906 + exbD b3005 0.15 -0.2 3,148,936 - exbD b3005 0.23 +0.4 3,148,992 - exbD b3005 0.36 +0.5 3,149,036 + exbD b3005 0.46 +0.5 3,149,092 + exbD b3005 0.59 -0.8 3,149,120 - +0.9 3,149,120 - exbD b3005 0.66 +1.9 3,149,131 + exbD b3005 0.68 +0.9 3,149,131 + exbD b3005 0.68 +1.3 3,149,208 + exbD b3005 0.86 +0.7 3,149,377 + exbB b3006 0.14 +0.3 3,149,377 + exbB b3006 0.14 -0.1 3,149,380 + exbB b3006 0.15 +1.1 3,149,396 - exbB b3006 0.17 +0.5 3,149,448 - exbB b3006 0.24 +1.0 3,149,501 + exbB b3006 0.31 +1.0 3,149,508 + exbB b3006 0.32 +2.0 3,149,543 - exbB b3006 0.37 +2.1 3,149,553 - exbB b3006 0.38 +1.0 3,149,554 + exbB b3006 0.38 +0.6 3,149,564 + exbB b3006 0.40 +1.6 3,149,659 + exbB b3006 0.53 +0.8 3,149,682 - exbB b3006 0.56 +0.5 3,149,682 - exbB b3006 0.56 +1.3 3,149,690 - exbB b3006 0.57 +1.2 3,149,707 + exbB b3006 0.59 +0.7 3,149,710 - exbB b3006 0.60 +0.8 3,149,718 + exbB b3006 0.61 +0.2 3,149,803 - exbB b3006 0.72 +0.2 3,149,803 - exbB b3006 0.72 +1.1 3,149,866 + exbB b3006 0.81 +1.0 3,149,956 + +0.6 3,149,956 + +0.2 3,149,956 + +0.7 3,149,983 + -1.6 3,150,016 - -1.2 3,150,257 - +1.0 3,150,288 - +0.8 3,150,292 + -1.1 3,150,300 - -2.1 3,150,300 - +0.2 3,150,337 - -2.2 3,150,378 - metC b3008 0.10 -0.6 3,150,421 + metC b3008 0.14 +1.4 3,150,421 + metC b3008 0.14 -1.2 3,150,514 - metC b3008 0.22 -0.1 3,150,575 + metC b3008 0.27 +0.3 3,150,577 - metC b3008 0.27 +0.6 3,150,583 - metC b3008 0.27 +0.4 3,150,640 - metC b3008 0.32 +0.7 3,150,654 + metC b3008 0.33 +0.5 3,150,669 + metC b3008 0.35 -0.3 3,150,706 - metC b3008 0.38 -0.2 3,150,791 - metC b3008 0.45 -0.2 3,150,820 + metC b3008 0.47 -0.5 3,150,820 + metC b3008 0.47 +0.1 3,150,912 + metC b3008 0.55 -0.9 3,150,914 + metC b3008 0.55 +1.8 3,150,915 + metC b3008 0.55 +1.4 3,150,932 - metC b3008 0.57 -0.5
Or see this region's nucleotide sequence