Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hypE and fhlA are separated by 73 nucleotides fhlA and ygbA are separated by 36 nucleotides ygbA and mutS are separated by 286 nucleotides
b2730: hypE - carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein (RefSeq), at 2,851,276 to 2,852,286
hypE
b2731: fhlA - DNA-binding transcriptional activator (NCBI), at 2,852,360 to 2,854,438
fhlA
b2732: ygbA - hypothetical protein (NCBI), at 2,854,475 to 2,854,828
ygbA
b2733: mutS - DNA mismatch repair protein (NCBI), at 2,855,115 to 2,857,676
mutS
Position (kb)
2852
2853
2854
2855 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2851.420 kb on - strand, within hypE at 2851.438 kb on - strand, within hypE at 2851.468 kb on + strand, within hypE at 2851.480 kb on + strand, within hypE at 2851.480 kb on + strand, within hypE at 2851.524 kb on - strand, within hypE at 2851.538 kb on + strand, within hypE at 2851.538 kb on + strand, within hypE at 2851.831 kb on - strand, within hypE at 2851.874 kb on + strand, within hypE at 2851.892 kb on - strand, within hypE at 2851.903 kb on + strand, within hypE at 2851.940 kb on - strand, within hypE at 2852.009 kb on - strand, within hypE at 2852.024 kb on + strand, within hypE at 2852.024 kb on + strand, within hypE at 2852.046 kb on - strand, within hypE at 2852.046 kb on - strand, within hypE at 2852.108 kb on - strand, within hypE at 2852.108 kb on - strand, within hypE at 2852.199 kb on - strand at 2852.199 kb on - strand at 2852.234 kb on - strand at 2852.234 kb on - strand at 2852.300 kb on + strand at 2852.300 kb on + strand at 2852.331 kb on + strand at 2852.331 kb on + strand at 2852.339 kb on - strand at 2852.364 kb on + strand at 2852.364 kb on + strand at 2852.428 kb on - strand at 2852.428 kb on - strand at 2852.457 kb on - strand at 2852.524 kb on + strand at 2852.612 kb on - strand, within fhlA at 2852.696 kb on - strand, within fhlA at 2852.796 kb on - strand, within fhlA at 2852.853 kb on + strand, within fhlA at 2852.866 kb on - strand, within fhlA at 2852.866 kb on - strand, within fhlA at 2852.896 kb on + strand, within fhlA at 2852.962 kb on + strand, within fhlA at 2852.962 kb on + strand, within fhlA at 2852.962 kb on + strand, within fhlA at 2852.990 kb on - strand, within fhlA at 2853.030 kb on + strand, within fhlA at 2853.167 kb on - strand, within fhlA at 2853.183 kb on + strand, within fhlA at 2853.183 kb on + strand, within fhlA at 2853.230 kb on - strand, within fhlA at 2853.251 kb on + strand, within fhlA at 2853.298 kb on - strand, within fhlA at 2853.298 kb on - strand, within fhlA at 2853.462 kb on - strand, within fhlA at 2853.518 kb on + strand, within fhlA at 2853.526 kb on - strand, within fhlA at 2853.526 kb on - strand, within fhlA at 2853.869 kb on - strand, within fhlA at 2854.040 kb on - strand, within fhlA at 2854.040 kb on - strand, within fhlA at 2854.115 kb on + strand, within fhlA at 2854.129 kb on - strand, within fhlA at 2854.131 kb on + strand, within fhlA at 2854.192 kb on - strand, within fhlA at 2854.260 kb on + strand at 2854.282 kb on - strand at 2854.529 kb on - strand, within ygbA at 2854.552 kb on + strand, within ygbA at 2854.586 kb on - strand, within ygbA at 2854.622 kb on - strand, within ygbA at 2854.652 kb on - strand, within ygbA at 2854.652 kb on - strand, within ygbA at 2854.984 kb on + strand at 2854.984 kb on + strand at 2855.167 kb on - strand at 2855.314 kb on + strand at 2855.314 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 2,851,420 - hypE b2730 0.14 -0.9 2,851,438 - hypE b2730 0.16 -1.0 2,851,468 + hypE b2730 0.19 -1.5 2,851,480 + hypE b2730 0.20 -1.7 2,851,480 + hypE b2730 0.20 -2.2 2,851,524 - hypE b2730 0.25 -0.4 2,851,538 + hypE b2730 0.26 +0.4 2,851,538 + hypE b2730 0.26 +2.3 2,851,831 - hypE b2730 0.55 -0.3 2,851,874 + hypE b2730 0.59 -1.5 2,851,892 - hypE b2730 0.61 -0.7 2,851,903 + hypE b2730 0.62 -0.3 2,851,940 - hypE b2730 0.66 +0.7 2,852,009 - hypE b2730 0.73 -1.3 2,852,024 + hypE b2730 0.74 -0.4 2,852,024 + hypE b2730 0.74 -1.7 2,852,046 - hypE b2730 0.76 -1.8 2,852,046 - hypE b2730 0.76 -0.1 2,852,108 - hypE b2730 0.82 -1.9 2,852,108 - hypE b2730 0.82 -0.8 2,852,199 - -0.5 2,852,199 - -2.6 2,852,234 - +1.3 2,852,234 - -0.8 2,852,300 + -1.1 2,852,300 + -0.9 2,852,331 + +0.3 2,852,331 + +0.2 2,852,339 - +0.7 2,852,364 + -0.1 2,852,364 + +0.8 2,852,428 - +0.7 2,852,428 - +0.9 2,852,457 - -0.2 2,852,524 + -0.2 2,852,612 - fhlA b2731 0.12 +1.1 2,852,696 - fhlA b2731 0.16 +0.2 2,852,796 - fhlA b2731 0.21 +0.6 2,852,853 + fhlA b2731 0.24 +0.7 2,852,866 - fhlA b2731 0.24 +0.2 2,852,866 - fhlA b2731 0.24 -0.3 2,852,896 + fhlA b2731 0.26 +0.5 2,852,962 + fhlA b2731 0.29 -2.2 2,852,962 + fhlA b2731 0.29 +0.2 2,852,962 + fhlA b2731 0.29 -0.1 2,852,990 - fhlA b2731 0.30 -0.1 2,853,030 + fhlA b2731 0.32 -0.3 2,853,167 - fhlA b2731 0.39 -0.0 2,853,183 + fhlA b2731 0.40 +0.0 2,853,183 + fhlA b2731 0.40 -1.6 2,853,230 - fhlA b2731 0.42 +0.8 2,853,251 + fhlA b2731 0.43 -0.3 2,853,298 - fhlA b2731 0.45 +1.2 2,853,298 - fhlA b2731 0.45 -0.6 2,853,462 - fhlA b2731 0.53 -0.1 2,853,518 + fhlA b2731 0.56 -0.3 2,853,526 - fhlA b2731 0.56 -0.0 2,853,526 - fhlA b2731 0.56 +0.4 2,853,869 - fhlA b2731 0.73 -0.4 2,854,040 - fhlA b2731 0.81 -1.8 2,854,040 - fhlA b2731 0.81 -0.6 2,854,115 + fhlA b2731 0.84 -2.8 2,854,129 - fhlA b2731 0.85 +0.1 2,854,131 + fhlA b2731 0.85 -1.1 2,854,192 - fhlA b2731 0.88 +2.2 2,854,260 + +0.5 2,854,282 - +0.1 2,854,529 - ygbA b2732 0.15 +0.1 2,854,552 + ygbA b2732 0.22 +0.4 2,854,586 - ygbA b2732 0.31 +0.0 2,854,622 - ygbA b2732 0.42 +0.2 2,854,652 - ygbA b2732 0.50 +0.1 2,854,652 - ygbA b2732 0.50 -0.4 2,854,984 + +0.6 2,854,984 + -1.8 2,855,167 - -1.1 2,855,314 + -0.8 2,855,314 + -0.6
Or see this region's nucleotide sequence