Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hypD and hypE overlap by 4 nucleotides hypE and fhlA are separated by 73 nucleotides
b2729: hypD - protein required for maturation of hydrogenases (NCBI), at 2,850,158 to 2,851,279
hypD
b2730: hypE - carbamoyl phosphate phosphatase, hydrogenase 3 maturation protein (RefSeq), at 2,851,276 to 2,852,286
hypE
b2731: fhlA - DNA-binding transcriptional activator (NCBI), at 2,852,360 to 2,854,438
fhlA
Position (kb)
2851
2852
2853 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2850.297 kb on - strand, within hypD at 2850.297 kb on - strand, within hypD at 2850.350 kb on - strand, within hypD at 2850.350 kb on - strand, within hypD at 2850.354 kb on + strand, within hypD at 2850.369 kb on - strand, within hypD at 2850.369 kb on - strand, within hypD at 2850.452 kb on + strand, within hypD at 2850.522 kb on - strand, within hypD at 2850.522 kb on - strand, within hypD at 2850.616 kb on - strand, within hypD at 2850.624 kb on - strand, within hypD at 2850.681 kb on + strand, within hypD at 2850.691 kb on - strand, within hypD at 2850.913 kb on - strand, within hypD at 2850.913 kb on - strand, within hypD at 2850.955 kb on - strand, within hypD at 2850.971 kb on + strand, within hypD at 2851.008 kb on + strand, within hypD at 2851.008 kb on + strand, within hypD at 2851.099 kb on + strand, within hypD at 2851.132 kb on + strand, within hypD at 2851.141 kb on - strand, within hypD at 2851.180 kb on + strand at 2851.180 kb on + strand at 2851.254 kb on - strand at 2851.318 kb on + strand at 2851.359 kb on - strand at 2851.359 kb on - strand at 2851.420 kb on - strand, within hypE at 2851.438 kb on - strand, within hypE at 2851.468 kb on + strand, within hypE at 2851.480 kb on + strand, within hypE at 2851.480 kb on + strand, within hypE at 2851.524 kb on - strand, within hypE at 2851.538 kb on + strand, within hypE at 2851.538 kb on + strand, within hypE at 2851.831 kb on - strand, within hypE at 2851.874 kb on + strand, within hypE at 2851.892 kb on - strand, within hypE at 2851.903 kb on + strand, within hypE at 2851.940 kb on - strand, within hypE at 2852.009 kb on - strand, within hypE at 2852.024 kb on + strand, within hypE at 2852.024 kb on + strand, within hypE at 2852.046 kb on - strand, within hypE at 2852.046 kb on - strand, within hypE at 2852.108 kb on - strand, within hypE at 2852.108 kb on - strand, within hypE at 2852.199 kb on - strand at 2852.199 kb on - strand at 2852.234 kb on - strand at 2852.234 kb on - strand at 2852.300 kb on + strand at 2852.300 kb on + strand at 2852.331 kb on + strand at 2852.331 kb on + strand at 2852.339 kb on - strand at 2852.364 kb on + strand at 2852.364 kb on + strand at 2852.428 kb on - strand at 2852.428 kb on - strand at 2852.457 kb on - strand at 2852.524 kb on + strand at 2852.612 kb on - strand, within fhlA at 2852.696 kb on - strand, within fhlA at 2852.796 kb on - strand, within fhlA at 2852.853 kb on + strand, within fhlA at 2852.866 kb on - strand, within fhlA at 2852.866 kb on - strand, within fhlA at 2852.896 kb on + strand, within fhlA at 2852.962 kb on + strand, within fhlA at 2852.962 kb on + strand, within fhlA at 2852.962 kb on + strand, within fhlA at 2852.990 kb on - strand, within fhlA at 2853.030 kb on + strand, within fhlA at 2853.167 kb on - strand, within fhlA at 2853.183 kb on + strand, within fhlA at 2853.183 kb on + strand, within fhlA at 2853.230 kb on - strand, within fhlA at 2853.251 kb on + strand, within fhlA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 2,850,297 - hypD b2729 0.12 -0.8 2,850,297 - hypD b2729 0.12 -1.4 2,850,350 - hypD b2729 0.17 -2.6 2,850,350 - hypD b2729 0.17 +0.4 2,850,354 + hypD b2729 0.17 +0.3 2,850,369 - hypD b2729 0.19 +0.0 2,850,369 - hypD b2729 0.19 -0.1 2,850,452 + hypD b2729 0.26 -0.8 2,850,522 - hypD b2729 0.32 -0.8 2,850,522 - hypD b2729 0.32 -0.5 2,850,616 - hypD b2729 0.41 -0.6 2,850,624 - hypD b2729 0.42 +1.0 2,850,681 + hypD b2729 0.47 -2.6 2,850,691 - hypD b2729 0.48 -1.2 2,850,913 - hypD b2729 0.67 -1.4 2,850,913 - hypD b2729 0.67 -2.2 2,850,955 - hypD b2729 0.71 -0.9 2,850,971 + hypD b2729 0.72 -1.0 2,851,008 + hypD b2729 0.76 +0.1 2,851,008 + hypD b2729 0.76 +0.0 2,851,099 + hypD b2729 0.84 -1.5 2,851,132 + hypD b2729 0.87 -2.5 2,851,141 - hypD b2729 0.88 -1.4 2,851,180 + -0.4 2,851,180 + -0.1 2,851,254 - -1.0 2,851,318 + -0.2 2,851,359 - -0.4 2,851,359 - +2.2 2,851,420 - hypE b2730 0.14 -0.9 2,851,438 - hypE b2730 0.16 -1.0 2,851,468 + hypE b2730 0.19 -1.5 2,851,480 + hypE b2730 0.20 -1.7 2,851,480 + hypE b2730 0.20 -2.2 2,851,524 - hypE b2730 0.25 -0.4 2,851,538 + hypE b2730 0.26 +0.4 2,851,538 + hypE b2730 0.26 +2.3 2,851,831 - hypE b2730 0.55 -0.3 2,851,874 + hypE b2730 0.59 -1.5 2,851,892 - hypE b2730 0.61 -0.7 2,851,903 + hypE b2730 0.62 -0.3 2,851,940 - hypE b2730 0.66 +0.7 2,852,009 - hypE b2730 0.73 -1.3 2,852,024 + hypE b2730 0.74 -0.4 2,852,024 + hypE b2730 0.74 -1.7 2,852,046 - hypE b2730 0.76 -1.8 2,852,046 - hypE b2730 0.76 -0.1 2,852,108 - hypE b2730 0.82 -1.9 2,852,108 - hypE b2730 0.82 -0.8 2,852,199 - -0.5 2,852,199 - -2.6 2,852,234 - +1.3 2,852,234 - -0.8 2,852,300 + -1.1 2,852,300 + -0.9 2,852,331 + +0.3 2,852,331 + +0.2 2,852,339 - +0.7 2,852,364 + -0.1 2,852,364 + +0.8 2,852,428 - +0.7 2,852,428 - +0.9 2,852,457 - -0.2 2,852,524 + -0.2 2,852,612 - fhlA b2731 0.12 +1.1 2,852,696 - fhlA b2731 0.16 +0.2 2,852,796 - fhlA b2731 0.21 +0.6 2,852,853 + fhlA b2731 0.24 +0.7 2,852,866 - fhlA b2731 0.24 +0.2 2,852,866 - fhlA b2731 0.24 -0.3 2,852,896 + fhlA b2731 0.26 +0.5 2,852,962 + fhlA b2731 0.29 -2.2 2,852,962 + fhlA b2731 0.29 +0.2 2,852,962 + fhlA b2731 0.29 -0.1 2,852,990 - fhlA b2731 0.30 -0.1 2,853,030 + fhlA b2731 0.32 -0.3 2,853,167 - fhlA b2731 0.39 -0.0 2,853,183 + fhlA b2731 0.40 +0.0 2,853,183 + fhlA b2731 0.40 -1.6 2,853,230 - fhlA b2731 0.42 +0.8 2,853,251 + fhlA b2731 0.43 -0.3
Or see this region's nucleotide sequence