Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hycA and hypA are separated by 211 nucleotides hypA and hypB are separated by 3 nucleotides hypB and hypC overlap by 10 nucleotides hypC and hypD overlap by 1 nucleotides
b2725: hycA - regulator of the transcriptional regulator FhlA (NCBI), at 2,847,996 to 2,848,457
hycA
b2726: hypA - protein involved in nickel insertion into hydrogenases 3 (NCBI), at 2,848,669 to 2,849,019
hypA
b2727: hypB - GTP hydrolase involved in nickel liganding into hydrogenases (NCBI), at 2,849,023 to 2,849,895
hypB
b2728: hypC - protein required for maturation of hydrogenases 1 and 3 (NCBI), at 2,849,886 to 2,850,158
hypC
b2729: hypD - protein required for maturation of hydrogenases (NCBI), at 2,850,158 to 2,851,279
hypD
Position (kb)
2849
2850 Strain fitness (log2 ratio)
-2
-1
0
1 at 2848.071 kb on - strand, within hycA at 2848.073 kb on - strand, within hycA at 2848.073 kb on - strand, within hycA at 2848.082 kb on - strand, within hycA at 2848.153 kb on - strand, within hycA at 2848.153 kb on - strand, within hycA at 2848.157 kb on + strand, within hycA at 2848.157 kb on + strand, within hycA at 2848.253 kb on - strand, within hycA at 2848.253 kb on - strand, within hycA at 2848.684 kb on - strand at 2848.684 kb on - strand at 2848.684 kb on - strand at 2848.719 kb on - strand, within hypA at 2848.850 kb on - strand, within hypA at 2848.892 kb on + strand, within hypA at 2848.912 kb on + strand, within hypA at 2848.944 kb on + strand, within hypA at 2848.965 kb on - strand, within hypA at 2848.965 kb on - strand, within hypA at 2849.025 kb on + strand at 2849.025 kb on + strand at 2849.290 kb on - strand, within hypB at 2849.290 kb on - strand, within hypB at 2849.345 kb on + strand, within hypB at 2849.390 kb on - strand, within hypB at 2849.430 kb on + strand, within hypB at 2849.430 kb on + strand, within hypB at 2849.486 kb on + strand, within hypB at 2849.486 kb on + strand, within hypB at 2849.494 kb on + strand, within hypB at 2849.507 kb on - strand, within hypB at 2849.581 kb on - strand, within hypB at 2849.748 kb on + strand, within hypB at 2849.884 kb on - strand at 2849.917 kb on + strand, within hypC at 2849.917 kb on + strand, within hypC at 2849.917 kb on - strand, within hypC at 2849.917 kb on - strand, within hypC at 2850.031 kb on - strand, within hypC at 2850.085 kb on + strand, within hypC at 2850.093 kb on - strand, within hypC at 2850.093 kb on - strand, within hypC at 2850.145 kb on - strand at 2850.170 kb on + strand at 2850.170 kb on + strand at 2850.222 kb on + strand at 2850.236 kb on + strand at 2850.246 kb on - strand at 2850.297 kb on - strand, within hypD at 2850.297 kb on - strand, within hypD at 2850.350 kb on - strand, within hypD at 2850.350 kb on - strand, within hypD at 2850.354 kb on + strand, within hypD at 2850.369 kb on - strand, within hypD at 2850.369 kb on - strand, within hypD at 2850.452 kb on + strand, within hypD at 2850.522 kb on - strand, within hypD at 2850.522 kb on - strand, within hypD at 2850.616 kb on - strand, within hypD at 2850.624 kb on - strand, within hypD at 2850.681 kb on + strand, within hypD at 2850.691 kb on - strand, within hypD
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 2,848,071 - hycA b2725 0.16 +0.6 2,848,073 - hycA b2725 0.17 -1.5 2,848,073 - hycA b2725 0.17 +0.7 2,848,082 - hycA b2725 0.19 +1.7 2,848,153 - hycA b2725 0.34 -1.4 2,848,153 - hycA b2725 0.34 +0.7 2,848,157 + hycA b2725 0.35 -1.1 2,848,157 + hycA b2725 0.35 -1.6 2,848,253 - hycA b2725 0.56 +0.9 2,848,253 - hycA b2725 0.56 +0.6 2,848,684 - +1.0 2,848,684 - +0.2 2,848,684 - -2.0 2,848,719 - hypA b2726 0.14 -0.1 2,848,850 - hypA b2726 0.52 +1.2 2,848,892 + hypA b2726 0.64 -0.5 2,848,912 + hypA b2726 0.69 -0.7 2,848,944 + hypA b2726 0.78 -2.3 2,848,965 - hypA b2726 0.84 +0.3 2,848,965 - hypA b2726 0.84 -0.0 2,849,025 + -0.4 2,849,025 + -0.5 2,849,290 - hypB b2727 0.31 -0.8 2,849,290 - hypB b2727 0.31 -2.2 2,849,345 + hypB b2727 0.37 -0.0 2,849,390 - hypB b2727 0.42 -0.1 2,849,430 + hypB b2727 0.47 -0.8 2,849,430 + hypB b2727 0.47 -1.6 2,849,486 + hypB b2727 0.53 -0.8 2,849,486 + hypB b2727 0.53 +0.5 2,849,494 + hypB b2727 0.54 +0.2 2,849,507 - hypB b2727 0.55 -2.2 2,849,581 - hypB b2727 0.64 -1.4 2,849,748 + hypB b2727 0.83 -2.6 2,849,884 - -1.4 2,849,917 + hypC b2728 0.11 -0.1 2,849,917 + hypC b2728 0.11 -1.3 2,849,917 - hypC b2728 0.11 -1.3 2,849,917 - hypC b2728 0.11 +0.1 2,850,031 - hypC b2728 0.53 +1.1 2,850,085 + hypC b2728 0.73 -0.8 2,850,093 - hypC b2728 0.76 -0.4 2,850,093 - hypC b2728 0.76 -0.4 2,850,145 - -1.1 2,850,170 + -1.4 2,850,170 + -0.1 2,850,222 + -0.5 2,850,236 + -1.7 2,850,246 - +0.6 2,850,297 - hypD b2729 0.12 -0.8 2,850,297 - hypD b2729 0.12 -1.4 2,850,350 - hypD b2729 0.17 -2.6 2,850,350 - hypD b2729 0.17 +0.4 2,850,354 + hypD b2729 0.17 +0.3 2,850,369 - hypD b2729 0.19 +0.0 2,850,369 - hypD b2729 0.19 -0.1 2,850,452 + hypD b2729 0.26 -0.8 2,850,522 - hypD b2729 0.32 -0.8 2,850,522 - hypD b2729 0.32 -0.5 2,850,616 - hypD b2729 0.41 -0.6 2,850,624 - hypD b2729 0.42 +1.0 2,850,681 + hypD b2729 0.47 -2.6 2,850,691 - hypD b2729 0.48 -1.2
Or see this region's nucleotide sequence