Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bcp and hyfA are separated by 252 nucleotides hyfA and hyfB overlap by 1 nucleotides hyfB and hyfC are separated by 10 nucleotides hyfC and hyfD are separated by 16 nucleotides
b2480: bcp - thioredoxin-dependent thiol peroxidase (NCBI), at 2,598,500 to 2,598,970
bcp
b2481: hyfA - hydrogenase 4 Fe-S subunit (VIMSS), at 2,599,223 to 2,599,840
hyfA
b2482: hyfB - NADH dehydrogenase subunit N (NCBI), at 2,599,840 to 2,601,858
hyfB
b2483: hyfC - hydrogenase 4 membrane subunit (VIMSS), at 2,601,869 to 2,602,816
hyfC
b2484: hyfD - hydrogenase 4 membrane subunit (NCBI), at 2,602,833 to 2,604,272
hyfD
Position (kb)
2599
2600
2601
2602 Strain fitness (log2 ratio)
-2
-1
0
1 at 2598.938 kb on + strand at 2598.967 kb on - strand at 2598.976 kb on - strand at 2599.005 kb on + strand at 2599.230 kb on + strand at 2599.238 kb on - strand at 2599.319 kb on - strand, within hyfA at 2599.319 kb on - strand, within hyfA at 2599.362 kb on + strand, within hyfA at 2599.370 kb on - strand, within hyfA at 2599.426 kb on + strand, within hyfA at 2599.488 kb on - strand, within hyfA at 2599.528 kb on - strand, within hyfA at 2599.913 kb on - strand at 2599.998 kb on + strand at 2600.132 kb on + strand, within hyfB at 2600.135 kb on - strand, within hyfB at 2600.340 kb on - strand, within hyfB at 2600.517 kb on - strand, within hyfB at 2600.527 kb on + strand, within hyfB at 2600.577 kb on - strand, within hyfB at 2600.621 kb on - strand, within hyfB at 2600.621 kb on - strand, within hyfB at 2600.759 kb on - strand, within hyfB at 2600.889 kb on + strand, within hyfB at 2601.194 kb on + strand, within hyfB at 2601.202 kb on - strand, within hyfB at 2601.245 kb on - strand, within hyfB at 2601.245 kb on - strand, within hyfB at 2601.404 kb on - strand, within hyfB at 2601.421 kb on + strand, within hyfB at 2601.434 kb on + strand, within hyfB at 2601.588 kb on + strand, within hyfB at 2601.644 kb on + strand, within hyfB at 2601.644 kb on + strand, within hyfB at 2601.652 kb on - strand, within hyfB at 2601.652 kb on - strand, within hyfB at 2601.701 kb on - strand at 2601.701 kb on - strand at 2601.810 kb on + strand at 2601.925 kb on + strand at 2601.925 kb on + strand at 2601.988 kb on - strand, within hyfC at 2602.127 kb on + strand, within hyfC at 2602.127 kb on + strand, within hyfC at 2602.150 kb on - strand, within hyfC at 2602.199 kb on - strand, within hyfC at 2602.199 kb on - strand, within hyfC at 2602.325 kb on + strand, within hyfC at 2602.350 kb on + strand, within hyfC at 2602.379 kb on + strand, within hyfC at 2602.382 kb on - strand, within hyfC at 2602.432 kb on - strand, within hyfC at 2602.620 kb on - strand, within hyfC at 2602.620 kb on - strand, within hyfC at 2602.813 kb on + strand at 2602.813 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 2,598,938 + +0.9 2,598,967 - +0.1 2,598,976 - +1.3 2,599,005 + +0.1 2,599,230 + +0.3 2,599,238 - +1.6 2,599,319 - hyfA b2481 0.16 -0.4 2,599,319 - hyfA b2481 0.16 +0.7 2,599,362 + hyfA b2481 0.22 +0.1 2,599,370 - hyfA b2481 0.24 -0.5 2,599,426 + hyfA b2481 0.33 +1.7 2,599,488 - hyfA b2481 0.43 +0.4 2,599,528 - hyfA b2481 0.49 +0.3 2,599,913 - +0.2 2,599,998 + -0.4 2,600,132 + hyfB b2482 0.14 +0.5 2,600,135 - hyfB b2482 0.15 +1.0 2,600,340 - hyfB b2482 0.25 +0.3 2,600,517 - hyfB b2482 0.34 -2.1 2,600,527 + hyfB b2482 0.34 +1.2 2,600,577 - hyfB b2482 0.37 +0.3 2,600,621 - hyfB b2482 0.39 -0.1 2,600,621 - hyfB b2482 0.39 -1.4 2,600,759 - hyfB b2482 0.46 +0.4 2,600,889 + hyfB b2482 0.52 +0.9 2,601,194 + hyfB b2482 0.67 -0.4 2,601,202 - hyfB b2482 0.67 +0.2 2,601,245 - hyfB b2482 0.70 -1.5 2,601,245 - hyfB b2482 0.70 +0.6 2,601,404 - hyfB b2482 0.77 -1.7 2,601,421 + hyfB b2482 0.78 +1.1 2,601,434 + hyfB b2482 0.79 +0.5 2,601,588 + hyfB b2482 0.87 -0.8 2,601,644 + hyfB b2482 0.89 +1.6 2,601,644 + hyfB b2482 0.89 +0.3 2,601,652 - hyfB b2482 0.90 +0.3 2,601,652 - hyfB b2482 0.90 -1.9 2,601,701 - -0.6 2,601,701 - +0.5 2,601,810 + +0.5 2,601,925 + -0.5 2,601,925 + -0.3 2,601,988 - hyfC b2483 0.13 +0.3 2,602,127 + hyfC b2483 0.27 -0.8 2,602,127 + hyfC b2483 0.27 -1.7 2,602,150 - hyfC b2483 0.30 -0.6 2,602,199 - hyfC b2483 0.35 -1.1 2,602,199 - hyfC b2483 0.35 +0.3 2,602,325 + hyfC b2483 0.48 -1.8 2,602,350 + hyfC b2483 0.51 -1.0 2,602,379 + hyfC b2483 0.54 +1.2 2,602,382 - hyfC b2483 0.54 +0.3 2,602,432 - hyfC b2483 0.59 -0.8 2,602,620 - hyfC b2483 0.79 +0.2 2,602,620 - hyfC b2483 0.79 +0.5 2,602,813 + -0.5 2,602,813 + +0.5
Or see this region's nucleotide sequence