Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutG and eutJ overlap by 11 nucleotides eutJ and eutE are separated by 10 nucleotides
b2453: eutG - ethanolamine utilization; homolog of Salmonella enzyme, similar to iron-containing alcohol dehydrogenase (VIMSS), at 2,566,346 to 2,567,533
eutG
b2454: eutJ - predicted chaperonin, ethanolamine utilization protein (NCBI), at 2,567,523 to 2,568,359
eutJ
b2455: eutE - predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI), at 2,568,370 to 2,569,773
eutE
Position (kb)
2567
2568
2569 Strain fitness (log2 ratio)
-2
-1
0
1 at 2566.585 kb on + strand, within eutG at 2566.620 kb on + strand, within eutG at 2566.620 kb on + strand, within eutG at 2566.637 kb on + strand, within eutG at 2566.894 kb on + strand, within eutG at 2566.914 kb on + strand, within eutG at 2566.938 kb on - strand, within eutG at 2566.938 kb on - strand, within eutG at 2566.938 kb on - strand, within eutG at 2567.008 kb on + strand, within eutG at 2567.012 kb on - strand, within eutG at 2567.130 kb on + strand, within eutG at 2567.210 kb on + strand, within eutG at 2567.220 kb on + strand, within eutG at 2567.220 kb on + strand, within eutG at 2567.241 kb on + strand, within eutG at 2567.241 kb on - strand, within eutG at 2567.325 kb on - strand, within eutG at 2567.325 kb on - strand, within eutG at 2567.325 kb on - strand, within eutG at 2567.325 kb on - strand, within eutG at 2567.341 kb on + strand, within eutG at 2567.341 kb on + strand, within eutG at 2567.418 kb on - strand at 2567.595 kb on + strand at 2567.707 kb on + strand, within eutJ at 2567.785 kb on + strand, within eutJ at 2567.785 kb on + strand, within eutJ at 2567.785 kb on + strand, within eutJ at 2567.963 kb on + strand, within eutJ at 2568.118 kb on - strand, within eutJ at 2568.140 kb on - strand, within eutJ at 2568.149 kb on + strand, within eutJ at 2568.149 kb on + strand, within eutJ at 2568.219 kb on + strand, within eutJ at 2568.298 kb on + strand at 2568.338 kb on + strand at 2568.891 kb on - strand, within eutE at 2568.932 kb on + strand, within eutE at 2568.932 kb on + strand, within eutE at 2568.937 kb on + strand, within eutE at 2568.937 kb on + strand, within eutE at 2569.130 kb on + strand, within eutE at 2569.252 kb on - strand, within eutE at 2569.252 kb on - strand, within eutE
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 2,566,585 + eutG b2453 0.20 -0.2 2,566,620 + eutG b2453 0.23 -1.6 2,566,620 + eutG b2453 0.23 -1.0 2,566,637 + eutG b2453 0.24 +0.0 2,566,894 + eutG b2453 0.46 +0.4 2,566,914 + eutG b2453 0.48 +0.4 2,566,938 - eutG b2453 0.50 -0.6 2,566,938 - eutG b2453 0.50 -0.1 2,566,938 - eutG b2453 0.50 +0.4 2,567,008 + eutG b2453 0.56 -1.2 2,567,012 - eutG b2453 0.56 +0.1 2,567,130 + eutG b2453 0.66 -2.3 2,567,210 + eutG b2453 0.73 -0.7 2,567,220 + eutG b2453 0.74 +1.5 2,567,220 + eutG b2453 0.74 -2.1 2,567,241 + eutG b2453 0.75 -0.2 2,567,241 - eutG b2453 0.75 -1.5 2,567,325 - eutG b2453 0.82 +0.8 2,567,325 - eutG b2453 0.82 -0.3 2,567,325 - eutG b2453 0.82 -0.2 2,567,325 - eutG b2453 0.82 -0.3 2,567,341 + eutG b2453 0.84 +0.2 2,567,341 + eutG b2453 0.84 +0.2 2,567,418 - -0.8 2,567,595 + +1.1 2,567,707 + eutJ b2454 0.22 +1.1 2,567,785 + eutJ b2454 0.31 +0.2 2,567,785 + eutJ b2454 0.31 +1.1 2,567,785 + eutJ b2454 0.31 +0.2 2,567,963 + eutJ b2454 0.53 -1.2 2,568,118 - eutJ b2454 0.71 +0.5 2,568,140 - eutJ b2454 0.74 -1.6 2,568,149 + eutJ b2454 0.75 -0.1 2,568,149 + eutJ b2454 0.75 -1.2 2,568,219 + eutJ b2454 0.83 +0.3 2,568,298 + -0.9 2,568,338 + +0.9 2,568,891 - eutE b2455 0.37 -0.1 2,568,932 + eutE b2455 0.40 +0.1 2,568,932 + eutE b2455 0.40 -0.4 2,568,937 + eutE b2455 0.40 -0.7 2,568,937 + eutE b2455 0.40 -0.3 2,569,130 + eutE b2455 0.54 -2.0 2,569,252 - eutE b2455 0.63 -1.2 2,569,252 - eutE b2455 0.63 -1.8
Or see this region's nucleotide sequence