Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lysP and yeiE are separated by 204 nucleotides yeiE and yeiH are separated by 98 nucleotides
b2156: lysP - lysine transporter (NCBI), at 2,245,085 to 2,246,554
lysP
b2157: yeiE - predicted DNA-binding transcriptional regulator (NCBI), at 2,246,759 to 2,247,640
yeiE
b2158: yeiH - conserved inner membrane protein (NCBI), at 2,247,739 to 2,248,788
yeiH
Position (kb)
2246
2247
2248 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2245.802 kb on - strand at 2245.873 kb on + strand, within lysP at 2245.888 kb on + strand, within lysP at 2245.937 kb on - strand, within lysP at 2245.985 kb on - strand, within lysP at 2246.012 kb on - strand, within lysP at 2246.012 kb on - strand, within lysP at 2246.040 kb on - strand, within lysP at 2246.092 kb on - strand, within lysP at 2246.092 kb on - strand, within lysP at 2246.189 kb on + strand, within lysP at 2246.189 kb on + strand, within lysP at 2246.197 kb on - strand, within lysP at 2246.218 kb on - strand, within lysP at 2246.218 kb on - strand, within lysP at 2246.253 kb on + strand, within lysP at 2246.253 kb on + strand, within lysP at 2246.344 kb on + strand, within lysP at 2246.513 kb on + strand at 2246.589 kb on + strand at 2246.760 kb on - strand at 2246.760 kb on - strand at 2246.844 kb on - strand at 2246.844 kb on - strand at 2246.962 kb on - strand, within yeiE at 2246.962 kb on - strand, within yeiE at 2246.979 kb on + strand, within yeiE at 2247.094 kb on + strand, within yeiE at 2247.199 kb on - strand, within yeiE at 2247.199 kb on - strand, within yeiE at 2247.236 kb on - strand, within yeiE at 2247.337 kb on + strand, within yeiE at 2247.346 kb on + strand, within yeiE at 2247.346 kb on + strand, within yeiE at 2247.351 kb on + strand, within yeiE at 2247.432 kb on - strand, within yeiE at 2247.432 kb on - strand, within yeiE at 2247.646 kb on - strand at 2247.712 kb on + strand at 2247.712 kb on + strand at 2247.729 kb on - strand at 2247.794 kb on + strand at 2247.814 kb on - strand at 2247.842 kb on - strand at 2247.869 kb on + strand, within yeiH at 2247.879 kb on + strand, within yeiH at 2247.879 kb on + strand, within yeiH at 2247.887 kb on - strand, within yeiH at 2247.904 kb on + strand, within yeiH at 2247.912 kb on - strand, within yeiH at 2247.912 kb on - strand, within yeiH at 2247.960 kb on - strand, within yeiH at 2248.074 kb on + strand, within yeiH at 2248.074 kb on + strand, within yeiH at 2248.114 kb on + strand, within yeiH at 2248.155 kb on + strand, within yeiH at 2248.161 kb on + strand, within yeiH at 2248.161 kb on + strand, within yeiH at 2248.167 kb on + strand, within yeiH at 2248.167 kb on + strand, within yeiH at 2248.185 kb on + strand, within yeiH at 2248.215 kb on + strand, within yeiH at 2248.282 kb on - strand, within yeiH at 2248.282 kb on - strand, within yeiH at 2248.445 kb on + strand, within yeiH at 2248.536 kb on - strand, within yeiH at 2248.536 kb on - strand, within yeiH at 2248.574 kb on + strand, within yeiH at 2248.607 kb on + strand, within yeiH
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 2,245,802 - -0.4 2,245,873 + lysP b2156 0.54 +1.6 2,245,888 + lysP b2156 0.55 -0.4 2,245,937 - lysP b2156 0.58 +1.3 2,245,985 - lysP b2156 0.61 -0.6 2,246,012 - lysP b2156 0.63 -0.7 2,246,012 - lysP b2156 0.63 -0.5 2,246,040 - lysP b2156 0.65 -0.4 2,246,092 - lysP b2156 0.69 -0.2 2,246,092 - lysP b2156 0.69 +1.4 2,246,189 + lysP b2156 0.75 +0.6 2,246,189 + lysP b2156 0.75 -0.7 2,246,197 - lysP b2156 0.76 -0.2 2,246,218 - lysP b2156 0.77 -1.1 2,246,218 - lysP b2156 0.77 -0.5 2,246,253 + lysP b2156 0.79 +0.6 2,246,253 + lysP b2156 0.79 +0.6 2,246,344 + lysP b2156 0.86 +0.4 2,246,513 + +0.2 2,246,589 + +0.0 2,246,760 - -0.4 2,246,760 - +0.2 2,246,844 - +0.0 2,246,844 - -0.2 2,246,962 - yeiE b2157 0.23 -1.6 2,246,962 - yeiE b2157 0.23 -1.7 2,246,979 + yeiE b2157 0.25 -0.9 2,247,094 + yeiE b2157 0.38 -1.3 2,247,199 - yeiE b2157 0.50 -2.7 2,247,199 - yeiE b2157 0.50 -2.8 2,247,236 - yeiE b2157 0.54 +0.3 2,247,337 + yeiE b2157 0.66 -2.0 2,247,346 + yeiE b2157 0.67 -1.8 2,247,346 + yeiE b2157 0.67 +1.5 2,247,351 + yeiE b2157 0.67 -1.5 2,247,432 - yeiE b2157 0.76 +0.8 2,247,432 - yeiE b2157 0.76 -1.7 2,247,646 - -0.3 2,247,712 + -2.0 2,247,712 + +0.0 2,247,729 - +0.8 2,247,794 + +0.1 2,247,814 - -0.3 2,247,842 - -2.1 2,247,869 + yeiH b2158 0.12 -1.5 2,247,879 + yeiH b2158 0.13 +0.8 2,247,879 + yeiH b2158 0.13 +0.2 2,247,887 - yeiH b2158 0.14 +1.3 2,247,904 + yeiH b2158 0.16 +0.7 2,247,912 - yeiH b2158 0.16 +1.0 2,247,912 - yeiH b2158 0.16 +1.3 2,247,960 - yeiH b2158 0.21 +0.8 2,248,074 + yeiH b2158 0.32 +0.5 2,248,074 + yeiH b2158 0.32 +0.2 2,248,114 + yeiH b2158 0.36 +0.9 2,248,155 + yeiH b2158 0.40 +0.3 2,248,161 + yeiH b2158 0.40 +1.0 2,248,161 + yeiH b2158 0.40 -0.6 2,248,167 + yeiH b2158 0.41 +0.2 2,248,167 + yeiH b2158 0.41 +0.4 2,248,185 + yeiH b2158 0.42 -2.6 2,248,215 + yeiH b2158 0.45 +0.1 2,248,282 - yeiH b2158 0.52 -0.0 2,248,282 - yeiH b2158 0.52 -0.5 2,248,445 + yeiH b2158 0.67 +0.4 2,248,536 - yeiH b2158 0.76 +0.7 2,248,536 - yeiH b2158 0.76 -0.3 2,248,574 + yeiH b2158 0.80 +0.3 2,248,607 + yeiH b2158 0.83 +0.7
Or see this region's nucleotide sequence