Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yedI and yedA are separated by 172 nucleotides yedA and vsr overlap by 12 nucleotides vsr and dcm overlap by 20 nucleotides
b1958: yedI - conserved inner membrane protein (NCBI), at 2,026,473 to 2,027,390
yedI
b1959: yedA - predicted inner membrane protein (NCBI), at 2,027,563 to 2,028,483
yedA
b1960: vsr - DNA mismatch endonuclease of very short patch repair (NCBI), at 2,028,472 to 2,028,942
vsr
b1961: dcm - DNA cytosine methylase (NCBI), at 2,028,923 to 2,030,341
dcm
Position (kb)
2027
2028
2029 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2026.610 kb on - strand, within yedI at 2026.657 kb on - strand, within yedI at 2026.834 kb on + strand, within yedI at 2026.859 kb on - strand, within yedI at 2026.880 kb on + strand, within yedI at 2026.906 kb on - strand, within yedI at 2027.009 kb on - strand, within yedI at 2027.080 kb on - strand, within yedI at 2027.285 kb on - strand, within yedI at 2027.321 kb on - strand at 2027.339 kb on - strand at 2027.339 kb on - strand at 2027.416 kb on - strand at 2027.416 kb on - strand at 2027.424 kb on - strand at 2027.450 kb on - strand at 2027.459 kb on - strand at 2027.608 kb on + strand at 2027.686 kb on + strand, within yedA at 2027.723 kb on + strand, within yedA at 2028.038 kb on + strand, within yedA at 2028.081 kb on - strand, within yedA at 2028.322 kb on + strand, within yedA at 2028.398 kb on + strand at 2028.717 kb on + strand, within vsr at 2028.727 kb on - strand, within vsr at 2028.727 kb on - strand, within vsr at 2028.753 kb on + strand, within vsr at 2028.753 kb on + strand, within vsr at 2028.819 kb on - strand, within vsr at 2028.819 kb on - strand, within vsr at 2028.943 kb on + strand at 2028.966 kb on + strand at 2028.966 kb on - strand at 2029.342 kb on + strand at 2029.361 kb on - strand, within dcm
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 2,026,610 - yedI b1958 0.15 -0.0 2,026,657 - yedI b1958 0.20 -0.8 2,026,834 + yedI b1958 0.39 -0.4 2,026,859 - yedI b1958 0.42 -0.1 2,026,880 + yedI b1958 0.44 -0.1 2,026,906 - yedI b1958 0.47 -1.4 2,027,009 - yedI b1958 0.58 +0.7 2,027,080 - yedI b1958 0.66 -2.0 2,027,285 - yedI b1958 0.88 -0.5 2,027,321 - +0.3 2,027,339 - -0.1 2,027,339 - -0.2 2,027,416 - -1.0 2,027,416 - +0.0 2,027,424 - -1.2 2,027,450 - -1.2 2,027,459 - +0.3 2,027,608 + -2.0 2,027,686 + yedA b1959 0.13 +0.3 2,027,723 + yedA b1959 0.17 +0.5 2,028,038 + yedA b1959 0.52 +0.2 2,028,081 - yedA b1959 0.56 +0.6 2,028,322 + yedA b1959 0.82 +1.8 2,028,398 + +1.0 2,028,717 + vsr b1960 0.52 +0.0 2,028,727 - vsr b1960 0.54 -0.4 2,028,727 - vsr b1960 0.54 +1.6 2,028,753 + vsr b1960 0.60 +0.0 2,028,753 + vsr b1960 0.60 -0.9 2,028,819 - vsr b1960 0.74 +0.6 2,028,819 - vsr b1960 0.74 -0.4 2,028,943 + -0.2 2,028,966 + -1.1 2,028,966 - -0.1 2,029,342 + +0.3 2,029,361 - dcm b1961 0.31 +0.0
Or see this region's nucleotide sequence