Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliP and fliQ are separated by 9 nucleotides fliQ and fliR are separated by 7 nucleotides fliR and rcsA are separated by 289 nucleotides rcsA and dsrB are separated by 43 nucleotides
b1948: fliP - flagellar biosynthesis protein P (NCBI), at 2,019,893 to 2,020,630
fliP
b1949: fliQ - flagellar biosynthesis protein Q (NCBI), at 2,020,640 to 2,020,909
fliQ
b1950: fliR - flagellar biosynthesis protein R (NCBI), at 2,020,917 to 2,021,702
fliR
b1951: rcsA - DNA-binding transcriptional activator, co-regulator with RcsB (NCBI), at 2,021,992 to 2,022,615
rcsA
b1952: dsrB - hypothetical protein (NCBI), at 2,022,659 to 2,022,847
dsrB
Position (kb)
2020
2021
2022 Strain fitness (log2 ratio)
-2
-1
0
1 at 2019.995 kb on + strand, within fliP at 2020.000 kb on + strand, within fliP at 2020.231 kb on - strand, within fliP at 2020.359 kb on - strand, within fliP at 2020.359 kb on - strand, within fliP at 2020.375 kb on + strand, within fliP at 2020.382 kb on - strand, within fliP at 2020.668 kb on + strand, within fliQ at 2020.719 kb on - strand, within fliQ at 2020.840 kb on + strand, within fliQ at 2020.894 kb on + strand at 2020.899 kb on - strand at 2020.950 kb on - strand at 2021.298 kb on + strand, within fliR at 2021.377 kb on + strand, within fliR at 2021.377 kb on + strand, within fliR at 2021.413 kb on - strand, within fliR at 2021.498 kb on + strand, within fliR at 2021.501 kb on + strand, within fliR at 2021.501 kb on + strand, within fliR at 2021.522 kb on - strand, within fliR at 2021.572 kb on + strand, within fliR at 2021.594 kb on + strand, within fliR at 2021.594 kb on + strand, within fliR at 2021.595 kb on + strand, within fliR at 2021.595 kb on + strand, within fliR at 2021.812 kb on - strand at 2021.982 kb on + strand at 2021.982 kb on + strand at 2022.049 kb on + strand at 2022.191 kb on + strand, within rcsA at 2022.382 kb on - strand, within rcsA at 2022.382 kb on - strand, within rcsA at 2022.413 kb on - strand, within rcsA at 2022.413 kb on - strand, within rcsA at 2022.424 kb on - strand, within rcsA at 2022.424 kb on - strand, within rcsA at 2022.427 kb on - strand, within rcsA at 2022.435 kb on + strand, within rcsA at 2022.453 kb on - strand, within rcsA at 2022.456 kb on - strand, within rcsA at 2022.610 kb on + strand at 2022.610 kb on + strand at 2022.627 kb on - strand at 2022.637 kb on - strand at 2022.650 kb on - strand at 2022.650 kb on - strand at 2022.674 kb on - strand at 2022.674 kb on - strand at 2022.694 kb on + strand, within dsrB at 2022.694 kb on + strand, within dsrB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 2,019,995 + fliP b1948 0.14 -0.4 2,020,000 + fliP b1948 0.14 +0.7 2,020,231 - fliP b1948 0.46 +0.7 2,020,359 - fliP b1948 0.63 -2.6 2,020,359 - fliP b1948 0.63 +0.0 2,020,375 + fliP b1948 0.65 -2.6 2,020,382 - fliP b1948 0.66 -0.5 2,020,668 + fliQ b1949 0.10 +1.2 2,020,719 - fliQ b1949 0.29 +0.8 2,020,840 + fliQ b1949 0.74 -0.2 2,020,894 + -0.1 2,020,899 - -2.6 2,020,950 - +0.0 2,021,298 + fliR b1950 0.48 +0.3 2,021,377 + fliR b1950 0.59 -1.0 2,021,377 + fliR b1950 0.59 +1.0 2,021,413 - fliR b1950 0.63 -0.4 2,021,498 + fliR b1950 0.74 +0.0 2,021,501 + fliR b1950 0.74 -2.0 2,021,501 + fliR b1950 0.74 -1.6 2,021,522 - fliR b1950 0.77 +1.6 2,021,572 + fliR b1950 0.83 +0.6 2,021,594 + fliR b1950 0.86 +1.6 2,021,594 + fliR b1950 0.86 +1.6 2,021,595 + fliR b1950 0.86 +0.0 2,021,595 + fliR b1950 0.86 +1.6 2,021,812 - +1.6 2,021,982 + +1.6 2,021,982 + +1.6 2,022,049 + -1.1 2,022,191 + rcsA b1951 0.32 +1.6 2,022,382 - rcsA b1951 0.62 +1.6 2,022,382 - rcsA b1951 0.62 +0.6 2,022,413 - rcsA b1951 0.67 +0.6 2,022,413 - rcsA b1951 0.67 +1.6 2,022,424 - rcsA b1951 0.69 +1.6 2,022,424 - rcsA b1951 0.69 +0.0 2,022,427 - rcsA b1951 0.70 +1.6 2,022,435 + rcsA b1951 0.71 +1.6 2,022,453 - rcsA b1951 0.74 -1.7 2,022,456 - rcsA b1951 0.74 +0.6 2,022,610 + +1.6 2,022,610 + -1.2 2,022,627 - +0.1 2,022,637 - -0.2 2,022,650 - +0.3 2,022,650 - +1.0 2,022,674 - +0.0 2,022,674 - -0.4 2,022,694 + dsrB b1952 0.19 -1.6 2,022,694 + dsrB b1952 0.19 -0.4
Or see this region's nucleotide sequence