Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sppA and ansA are separated by 166 nucleotides ansA and ydjB are separated by 10 nucleotides ydjB and ydjE are separated by 92 nucleotides
b1766: sppA - protease IV (signal peptide peptidase) (NCBI), at 1,846,861 to 1,848,717
sppA
b1767: ansA - cytoplasmic asparaginase I (NCBI), at 1,848,884 to 1,849,900
ansA
b1768: ydjB - orf, hypothetical protein (VIMSS), at 1,849,911 to 1,850,552
ydjB
b1769: ydjE - predicted transporter (NCBI), at 1,850,645 to 1,852,003
ydjE
Position (kb)
1848
1849
1850 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1847.975 kb on + strand, within sppA at 1848.206 kb on + strand, within sppA at 1848.219 kb on - strand, within sppA at 1848.280 kb on + strand, within sppA at 1848.374 kb on + strand, within sppA at 1848.374 kb on + strand, within sppA at 1848.390 kb on + strand, within sppA at 1848.390 kb on + strand, within sppA at 1848.398 kb on - strand, within sppA at 1848.726 kb on - strand at 1848.833 kb on - strand at 1848.833 kb on - strand at 1848.842 kb on + strand at 1848.842 kb on + strand at 1848.850 kb on - strand at 1848.979 kb on - strand at 1848.979 kb on - strand at 1849.035 kb on - strand, within ansA at 1849.123 kb on + strand, within ansA at 1849.152 kb on + strand, within ansA at 1849.152 kb on + strand, within ansA at 1849.160 kb on - strand, within ansA at 1849.160 kb on - strand, within ansA at 1849.173 kb on - strand, within ansA at 1849.184 kb on - strand, within ansA at 1849.260 kb on - strand, within ansA at 1849.474 kb on - strand, within ansA at 1849.474 kb on - strand, within ansA at 1849.603 kb on + strand, within ansA at 1849.751 kb on + strand, within ansA at 1849.785 kb on + strand, within ansA at 1849.896 kb on + strand at 1849.896 kb on + strand at 1850.057 kb on - strand, within ydjB at 1850.262 kb on + strand, within ydjB at 1850.272 kb on - strand, within ydjB at 1850.313 kb on + strand, within ydjB at 1850.352 kb on + strand, within ydjB at 1850.353 kb on + strand, within ydjB at 1850.365 kb on + strand, within ydjB at 1850.365 kb on + strand, within ydjB at 1850.367 kb on + strand, within ydjB at 1850.372 kb on + strand, within ydjB at 1850.372 kb on + strand, within ydjB at 1850.463 kb on + strand, within ydjB at 1850.463 kb on + strand, within ydjB at 1850.463 kb on + strand, within ydjB at 1850.484 kb on + strand, within ydjB at 1850.523 kb on + strand at 1850.552 kb on + strand at 1850.552 kb on + strand at 1850.567 kb on + strand at 1850.567 kb on + strand at 1850.570 kb on + strand at 1850.570 kb on + strand at 1850.669 kb on + strand at 1850.718 kb on - strand at 1850.718 kb on - strand at 1850.720 kb on + strand at 1850.728 kb on + strand at 1850.728 kb on + strand at 1850.728 kb on - strand at 1850.738 kb on + strand at 1850.752 kb on - strand at 1850.795 kb on - strand, within ydjE at 1850.795 kb on - strand, within ydjE at 1850.795 kb on - strand, within ydjE at 1850.796 kb on + strand, within ydjE at 1850.796 kb on + strand, within ydjE at 1850.803 kb on + strand, within ydjE at 1850.804 kb on - strand, within ydjE at 1850.812 kb on - strand, within ydjE at 1850.816 kb on - strand, within ydjE at 1850.836 kb on + strand, within ydjE at 1850.851 kb on + strand, within ydjE at 1850.852 kb on + strand, within ydjE at 1850.853 kb on + strand, within ydjE at 1850.854 kb on + strand, within ydjE
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 1,847,975 + sppA b1766 0.60 +0.4 1,848,206 + sppA b1766 0.72 +0.5 1,848,219 - sppA b1766 0.73 +0.5 1,848,280 + sppA b1766 0.76 -1.8 1,848,374 + sppA b1766 0.81 -0.3 1,848,374 + sppA b1766 0.81 +0.7 1,848,390 + sppA b1766 0.82 +1.0 1,848,390 + sppA b1766 0.82 +0.1 1,848,398 - sppA b1766 0.83 -0.6 1,848,726 - +0.5 1,848,833 - +1.2 1,848,833 - -1.7 1,848,842 + -0.2 1,848,842 + -0.2 1,848,850 - +0.1 1,848,979 - -1.4 1,848,979 - -0.1 1,849,035 - ansA b1767 0.15 -0.5 1,849,123 + ansA b1767 0.24 +1.1 1,849,152 + ansA b1767 0.26 -0.3 1,849,152 + ansA b1767 0.26 -0.5 1,849,160 - ansA b1767 0.27 -0.1 1,849,160 - ansA b1767 0.27 +1.6 1,849,173 - ansA b1767 0.28 +0.8 1,849,184 - ansA b1767 0.29 +0.5 1,849,260 - ansA b1767 0.37 +0.3 1,849,474 - ansA b1767 0.58 -0.1 1,849,474 - ansA b1767 0.58 +1.0 1,849,603 + ansA b1767 0.71 -0.6 1,849,751 + ansA b1767 0.85 -1.8 1,849,785 + ansA b1767 0.89 +0.6 1,849,896 + -0.1 1,849,896 + +0.3 1,850,057 - ydjB b1768 0.23 +0.4 1,850,262 + ydjB b1768 0.55 +0.3 1,850,272 - ydjB b1768 0.56 -1.2 1,850,313 + ydjB b1768 0.63 +1.1 1,850,352 + ydjB b1768 0.69 -1.3 1,850,353 + ydjB b1768 0.69 -0.3 1,850,365 + ydjB b1768 0.71 +0.5 1,850,365 + ydjB b1768 0.71 +0.2 1,850,367 + ydjB b1768 0.71 -0.6 1,850,372 + ydjB b1768 0.72 +0.3 1,850,372 + ydjB b1768 0.72 -0.2 1,850,463 + ydjB b1768 0.86 +1.8 1,850,463 + ydjB b1768 0.86 -0.2 1,850,463 + ydjB b1768 0.86 -1.2 1,850,484 + ydjB b1768 0.89 +0.2 1,850,523 + +0.1 1,850,552 + +0.1 1,850,552 + +1.1 1,850,567 + -0.8 1,850,567 + -1.6 1,850,570 + +1.1 1,850,570 + -0.6 1,850,669 + -2.0 1,850,718 - -0.8 1,850,718 - -1.6 1,850,720 + +0.3 1,850,728 + -0.8 1,850,728 + -0.4 1,850,728 - +1.1 1,850,738 + -0.1 1,850,752 - -1.6 1,850,795 - ydjE b1769 0.11 +1.2 1,850,795 - ydjE b1769 0.11 -0.3 1,850,795 - ydjE b1769 0.11 -0.4 1,850,796 + ydjE b1769 0.11 -0.8 1,850,796 + ydjE b1769 0.11 -0.8 1,850,803 + ydjE b1769 0.12 -0.7 1,850,804 - ydjE b1769 0.12 -1.4 1,850,812 - ydjE b1769 0.12 -1.2 1,850,816 - ydjE b1769 0.13 +1.6 1,850,836 + ydjE b1769 0.14 -0.6 1,850,851 + ydjE b1769 0.15 +0.6 1,850,852 + ydjE b1769 0.15 +0.6 1,850,853 + ydjE b1769 0.15 +1.6 1,850,854 + ydjE b1769 0.15 +1.6
Or see this region's nucleotide sequence