Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydgT and b1626 are separated by 85 nucleotides b1626 and rsxA are separated by 76 nucleotides rsxA and rsxB overlap by 1 nucleotides rsxB and rsxC overlap by 8 nucleotides
b1625: ydgT - predicted regulator (NCBI), at 1,702,973 to 1,703,188
ydgT
b1626: b1626 - orf, hypothetical protein (VIMSS), at 1,703,274 to 1,703,714
b1626
b1627: rsxA - Na(+)-translocating NADH-quinone reductase subunit E (NCBI), at 1,703,791 to 1,704,372
rsxA
b1628: rsxB - electron transport complex protein RnfB (NCBI), at 1,704,372 to 1,704,950
rsxB
b1629: rsxC - electron transport complex protein RnfC (NCBI), at 1,704,943 to 1,707,165
rsxC
Position (kb)
1703
1704
1705 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1702.812 kb on + strand at 1702.812 kb on + strand at 1702.821 kb on + strand at 1702.979 kb on + strand at 1702.984 kb on + strand at 1702.984 kb on + strand at 1702.987 kb on - strand at 1702.987 kb on - strand at 1702.987 kb on - strand at 1702.990 kb on - strand at 1702.992 kb on - strand at 1703.033 kb on - strand, within ydgT at 1703.052 kb on + strand, within ydgT at 1703.054 kb on - strand, within ydgT at 1703.054 kb on - strand, within ydgT at 1703.055 kb on + strand, within ydgT at 1703.070 kb on + strand, within ydgT at 1703.070 kb on + strand, within ydgT at 1703.070 kb on + strand, within ydgT at 1703.070 kb on + strand, within ydgT at 1703.070 kb on + strand, within ydgT at 1703.078 kb on - strand, within ydgT at 1703.078 kb on - strand, within ydgT at 1703.081 kb on + strand, within ydgT at 1703.084 kb on + strand, within ydgT at 1703.089 kb on + strand, within ydgT at 1703.092 kb on - strand, within ydgT at 1703.102 kb on - strand, within ydgT at 1703.105 kb on - strand, within ydgT at 1703.109 kb on + strand, within ydgT at 1703.109 kb on + strand, within ydgT at 1703.142 kb on - strand, within ydgT at 1703.153 kb on - strand, within ydgT at 1703.178 kb on + strand at 1703.210 kb on - strand at 1703.263 kb on - strand at 1703.268 kb on - strand at 1703.276 kb on + strand at 1703.278 kb on - strand at 1703.296 kb on + strand at 1703.296 kb on + strand at 1703.368 kb on - strand, within b1626 at 1703.368 kb on - strand, within b1626 at 1703.369 kb on + strand, within b1626 at 1703.377 kb on - strand, within b1626 at 1703.377 kb on - strand, within b1626 at 1703.377 kb on - strand, within b1626 at 1703.380 kb on - strand, within b1626 at 1703.380 kb on - strand, within b1626 at 1703.424 kb on + strand, within b1626 at 1703.427 kb on - strand, within b1626 at 1703.427 kb on - strand, within b1626 at 1703.480 kb on + strand, within b1626 at 1703.495 kb on + strand, within b1626 at 1703.503 kb on - strand, within b1626 at 1703.518 kb on - strand, within b1626 at 1703.518 kb on - strand, within b1626 at 1703.527 kb on + strand, within b1626 at 1703.537 kb on + strand, within b1626 at 1703.542 kb on + strand, within b1626 at 1703.542 kb on + strand, within b1626 at 1703.549 kb on - strand, within b1626 at 1703.609 kb on + strand, within b1626 at 1703.617 kb on - strand, within b1626 at 1703.670 kb on + strand, within b1626 at 1703.671 kb on - strand at 1703.671 kb on - strand at 1703.819 kb on + strand at 1703.827 kb on - strand at 1703.845 kb on - strand at 1703.847 kb on + strand at 1703.861 kb on + strand, within rsxA at 1703.861 kb on + strand, within rsxA at 1703.908 kb on - strand, within rsxA at 1704.048 kb on + strand, within rsxA at 1704.090 kb on - strand, within rsxA at 1704.235 kb on - strand, within rsxA at 1704.376 kb on + strand at 1704.376 kb on + strand at 1704.377 kb on - strand at 1704.525 kb on - strand, within rsxB at 1704.772 kb on + strand, within rsxB at 1704.806 kb on + strand, within rsxB at 1705.039 kb on + strand at 1705.042 kb on - strand at 1705.235 kb on - strand, within rsxC at 1705.290 kb on + strand, within rsxC at 1705.290 kb on + strand, within rsxC at 1705.308 kb on - strand, within rsxC at 1705.308 kb on - strand, within rsxC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 1,702,812 + -1.2 1,702,812 + -1.5 1,702,821 + -0.4 1,702,979 + -1.3 1,702,984 + +0.0 1,702,984 + +0.0 1,702,987 - -1.6 1,702,987 - +0.6 1,702,987 - +0.0 1,702,990 - -0.7 1,702,992 - +2.3 1,703,033 - ydgT b1625 0.28 +1.0 1,703,052 + ydgT b1625 0.37 +0.8 1,703,054 - ydgT b1625 0.38 -0.1 1,703,054 - ydgT b1625 0.38 -0.4 1,703,055 + ydgT b1625 0.38 -1.6 1,703,070 + ydgT b1625 0.45 +0.4 1,703,070 + ydgT b1625 0.45 +0.6 1,703,070 + ydgT b1625 0.45 +0.8 1,703,070 + ydgT b1625 0.45 -0.2 1,703,070 + ydgT b1625 0.45 -1.3 1,703,078 - ydgT b1625 0.49 +0.0 1,703,078 - ydgT b1625 0.49 -1.9 1,703,081 + ydgT b1625 0.50 +0.5 1,703,084 + ydgT b1625 0.51 +0.7 1,703,089 + ydgT b1625 0.54 +0.4 1,703,092 - ydgT b1625 0.55 +1.5 1,703,102 - ydgT b1625 0.60 -2.2 1,703,105 - ydgT b1625 0.61 -0.9 1,703,109 + ydgT b1625 0.63 +1.0 1,703,109 + ydgT b1625 0.63 -1.7 1,703,142 - ydgT b1625 0.78 -1.2 1,703,153 - ydgT b1625 0.83 -3.3 1,703,178 + -2.6 1,703,210 - +0.8 1,703,263 - +0.0 1,703,268 - -0.4 1,703,276 + +0.0 1,703,278 - -1.1 1,703,296 + -1.0 1,703,296 + +1.2 1,703,368 - b1626 b1626 0.21 +0.5 1,703,368 - b1626 b1626 0.21 -0.4 1,703,369 + b1626 b1626 0.22 +0.0 1,703,377 - b1626 b1626 0.23 -1.4 1,703,377 - b1626 b1626 0.23 +0.9 1,703,377 - b1626 b1626 0.23 -0.6 1,703,380 - b1626 b1626 0.24 +1.6 1,703,380 - b1626 b1626 0.24 -0.4 1,703,424 + b1626 b1626 0.34 +1.3 1,703,427 - b1626 b1626 0.35 +2.0 1,703,427 - b1626 b1626 0.35 +1.6 1,703,480 + b1626 b1626 0.47 +0.4 1,703,495 + b1626 b1626 0.50 -1.0 1,703,503 - b1626 b1626 0.52 -1.3 1,703,518 - b1626 b1626 0.55 -0.1 1,703,518 - b1626 b1626 0.55 -0.1 1,703,527 + b1626 b1626 0.57 +0.2 1,703,537 + b1626 b1626 0.60 -0.7 1,703,542 + b1626 b1626 0.61 +1.0 1,703,542 + b1626 b1626 0.61 -0.8 1,703,549 - b1626 b1626 0.62 -0.5 1,703,609 + b1626 b1626 0.76 -0.6 1,703,617 - b1626 b1626 0.78 -0.2 1,703,670 + b1626 b1626 0.90 -1.4 1,703,671 - +0.7 1,703,671 - -1.4 1,703,819 + +2.8 1,703,827 - +2.1 1,703,845 - +1.6 1,703,847 + +2.1 1,703,861 + rsxA b1627 0.12 +0.9 1,703,861 + rsxA b1627 0.12 +1.8 1,703,908 - rsxA b1627 0.20 +2.4 1,704,048 + rsxA b1627 0.44 +2.9 1,704,090 - rsxA b1627 0.51 +2.1 1,704,235 - rsxA b1627 0.76 +2.1 1,704,376 + +0.2 1,704,376 + +1.4 1,704,377 - +0.2 1,704,525 - rsxB b1628 0.26 -1.0 1,704,772 + rsxB b1628 0.69 +1.6 1,704,806 + rsxB b1628 0.75 +1.4 1,705,039 + +2.7 1,705,042 - +2.2 1,705,235 - rsxC b1629 0.13 -0.2 1,705,290 + rsxC b1629 0.16 +1.1 1,705,290 + rsxC b1629 0.16 -1.2 1,705,308 - rsxC b1629 0.16 +2.5 1,705,308 - rsxC b1629 0.16 +2.7
Or see this region's nucleotide sequence