Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ynfC and b1586 are separated by 107 nucleotides b1586 and ynfE are separated by 198 nucleotides ynfE and ynfF are separated by 60 nucleotides
b1585: ynfC - orf, hypothetical protein (VIMSS), at 1,654,771 to 1,655,481
ynfC
b1586: b1586 - orf, hypothetical protein (VIMSS), at 1,655,589 to 1,655,894
b1586
b1587: ynfE - oxidoreductase subunit (NCBI), at 1,656,093 to 1,658,519
ynfE
b1588: ynfF - oxidoreductase subunit (RefSeq), at 1,658,580 to 1,661,003
ynfF
Position (kb)
1656
1657
1658
1659 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1655.102 kb on + strand, within ynfC at 1655.114 kb on + strand, within ynfC at 1655.123 kb on - strand, within ynfC at 1655.123 kb on - strand, within ynfC at 1655.147 kb on - strand, within ynfC at 1655.175 kb on - strand, within ynfC at 1655.199 kb on - strand, within ynfC at 1655.208 kb on + strand, within ynfC at 1655.216 kb on - strand, within ynfC at 1655.394 kb on + strand, within ynfC at 1655.402 kb on - strand at 1655.402 kb on - strand, within ynfC at 1655.413 kb on - strand at 1655.428 kb on + strand at 1655.500 kb on - strand at 1655.500 kb on - strand at 1655.626 kb on + strand, within b1586 at 1655.634 kb on - strand, within b1586 at 1655.643 kb on + strand, within b1586 at 1655.645 kb on + strand, within b1586 at 1655.655 kb on + strand, within b1586 at 1655.655 kb on + strand, within b1586 at 1655.786 kb on - strand, within b1586 at 1655.786 kb on - strand, within b1586 at 1655.841 kb on + strand, within b1586 at 1655.858 kb on + strand, within b1586 at 1655.955 kb on - strand at 1656.025 kb on - strand at 1656.291 kb on - strand at 1656.291 kb on - strand at 1656.418 kb on - strand, within ynfE at 1656.569 kb on - strand, within ynfE at 1656.673 kb on - strand, within ynfE at 1656.712 kb on - strand, within ynfE at 1656.712 kb on - strand, within ynfE at 1656.800 kb on + strand, within ynfE at 1656.800 kb on + strand, within ynfE at 1656.944 kb on + strand, within ynfE at 1656.970 kb on - strand, within ynfE at 1657.604 kb on + strand, within ynfE at 1657.707 kb on - strand, within ynfE at 1657.762 kb on + strand, within ynfE at 1657.892 kb on + strand, within ynfE at 1658.038 kb on - strand, within ynfE at 1658.056 kb on + strand, within ynfE at 1658.249 kb on + strand, within ynfE at 1658.251 kb on - strand, within ynfE at 1658.251 kb on - strand, within ynfE at 1658.314 kb on + strand at 1658.488 kb on + strand at 1658.579 kb on + strand at 1658.579 kb on + strand at 1658.750 kb on + strand at 1658.750 kb on + strand at 1658.992 kb on + strand, within ynfF at 1659.171 kb on - strand, within ynfF at 1659.174 kb on - strand, within ynfF at 1659.176 kb on - strand, within ynfF at 1659.176 kb on - strand, within ynfF at 1659.475 kb on - strand, within ynfF
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 1,655,102 + ynfC b1585 0.47 +0.6 1,655,114 + ynfC b1585 0.48 +1.8 1,655,123 - ynfC b1585 0.50 -0.8 1,655,123 - ynfC b1585 0.50 +0.2 1,655,147 - ynfC b1585 0.53 -0.7 1,655,175 - ynfC b1585 0.57 -0.7 1,655,199 - ynfC b1585 0.60 -2.3 1,655,208 + ynfC b1585 0.61 +1.6 1,655,216 - ynfC b1585 0.63 +0.6 1,655,394 + ynfC b1585 0.88 +0.6 1,655,402 - +0.0 1,655,402 - ynfC b1585 0.89 +0.3 1,655,413 - +1.4 1,655,428 + +0.1 1,655,500 - +1.0 1,655,500 - -0.6 1,655,626 + b1586 b1586 0.12 +1.8 1,655,634 - b1586 b1586 0.15 +0.3 1,655,643 + b1586 b1586 0.18 +0.3 1,655,645 + b1586 b1586 0.18 +0.5 1,655,655 + b1586 b1586 0.22 -0.7 1,655,655 + b1586 b1586 0.22 -0.6 1,655,786 - b1586 b1586 0.64 -1.7 1,655,786 - b1586 b1586 0.64 +0.6 1,655,841 + b1586 b1586 0.82 +1.4 1,655,858 + b1586 b1586 0.88 -0.3 1,655,955 - -0.2 1,656,025 - -1.3 1,656,291 - +1.4 1,656,291 - +0.1 1,656,418 - ynfE b1587 0.13 +0.2 1,656,569 - ynfE b1587 0.20 +0.8 1,656,673 - ynfE b1587 0.24 +0.4 1,656,712 - ynfE b1587 0.26 -1.2 1,656,712 - ynfE b1587 0.26 +0.9 1,656,800 + ynfE b1587 0.29 +0.6 1,656,800 + ynfE b1587 0.29 +1.8 1,656,944 + ynfE b1587 0.35 +1.3 1,656,970 - ynfE b1587 0.36 -1.0 1,657,604 + ynfE b1587 0.62 +1.9 1,657,707 - ynfE b1587 0.67 -0.4 1,657,762 + ynfE b1587 0.69 +0.8 1,657,892 + ynfE b1587 0.74 -1.6 1,658,038 - ynfE b1587 0.80 -1.8 1,658,056 + ynfE b1587 0.81 +0.5 1,658,249 + ynfE b1587 0.89 +0.8 1,658,251 - ynfE b1587 0.89 -0.5 1,658,251 - ynfE b1587 0.89 +0.5 1,658,314 + +2.7 1,658,488 + +3.6 1,658,579 + -0.2 1,658,579 + -0.0 1,658,750 + -0.5 1,658,750 + +1.5 1,658,992 + ynfF b1588 0.17 -2.4 1,659,171 - ynfF b1588 0.24 -1.0 1,659,174 - ynfF b1588 0.25 -0.8 1,659,176 - ynfF b1588 0.25 +0.4 1,659,176 - ynfF b1588 0.25 +0.4 1,659,475 - ynfF b1588 0.37 +0.2
Or see this region's nucleotide sequence