Experiment: LB Anaerobic with Phenazine ethosulfate 0.000125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybeX and ybeY are separated by 89 nucleotides ybeY and ybeZ overlap by 4 nucleotides ybeZ and miaB are separated by 152 nucleotides
b0658: ybeX - predicteed ion transport (NCBI), at 690,129 to 691,007
ybeX
b0659: ybeY - hypothetical protein (NCBI), at 691,097 to 691,564
ybeY
b0660: ybeZ - predicted protein with nucleoside triphosphate hydrolase domain (RefSeq), at 691,561 to 692,601
ybeZ
b0661: miaB - isopentenyl-adenosine A37 tRNA methylthiolase (NCBI), at 692,754 to 694,178
miaB
Position (kb) 691
692
693 Strain fitness (log2 ratio) -2
-1
0
1 at 690.589 kb on - strand, within ybeX at 690.609 kb on - strand, within ybeX at 690.713 kb on - strand, within ybeX at 690.779 kb on - strand, within ybeX at 690.795 kb on - strand, within ybeX at 690.795 kb on - strand, within ybeX at 690.809 kb on + strand, within ybeX at 690.817 kb on - strand, within ybeX at 691.017 kb on - strand at 691.646 kb on - strand at 691.668 kb on - strand, within ybeZ at 691.757 kb on + strand, within ybeZ at 691.757 kb on + strand, within ybeZ at 691.766 kb on - strand, within ybeZ at 691.793 kb on - strand, within ybeZ at 691.793 kb on - strand, within ybeZ at 691.842 kb on + strand, within ybeZ at 691.849 kb on - strand, within ybeZ at 692.300 kb on - strand, within ybeZ at 692.300 kb on - strand, within ybeZ at 692.407 kb on - strand, within ybeZ at 692.407 kb on - strand, within ybeZ at 692.576 kb on - strand at 692.576 kb on - strand at 692.598 kb on - strand at 692.598 kb on - strand at 692.774 kb on + strand at 692.914 kb on + strand, within miaB at 693.086 kb on + strand, within miaB at 693.088 kb on + strand, within miaB at 693.088 kb on + strand, within miaB at 693.141 kb on + strand, within miaB at 693.175 kb on + strand, within miaB at 693.175 kb on - strand, within miaB at 693.175 kb on - strand, within miaB at 693.183 kb on - strand, within miaB at 693.183 kb on - strand, within miaB at 693.236 kb on - strand, within miaB at 693.302 kb on + strand, within miaB at 693.310 kb on - strand, within miaB at 693.310 kb on - strand, within miaB at 693.310 kb on - strand, within miaB at 693.310 kb on - strand, within miaB at 693.337 kb on - strand, within miaB at 693.380 kb on + strand, within miaB at 693.380 kb on + strand, within miaB at 693.380 kb on + strand, within miaB at 693.595 kb on - strand, within miaB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Phenazine ethosulfate 0.000125 mM remove 690,589 - ybeX b0658 0.52 +1.6 690,609 - ybeX b0658 0.55 +1.6 690,713 - ybeX b0658 0.66 +1.6 690,779 - ybeX b0658 0.74 +1.6 690,795 - ybeX b0658 0.76 +1.6 690,795 - ybeX b0658 0.76 +1.6 690,809 + ybeX b0658 0.77 +1.6 690,817 - ybeX b0658 0.78 +1.6 691,017 - +0.4 691,646 - -0.1 691,668 - ybeZ b0660 0.10 -1.8 691,757 + ybeZ b0660 0.19 -0.9 691,757 + ybeZ b0660 0.19 -0.6 691,766 - ybeZ b0660 0.20 -1.4 691,793 - ybeZ b0660 0.22 -1.7 691,793 - ybeZ b0660 0.22 -0.9 691,842 + ybeZ b0660 0.27 +0.2 691,849 - ybeZ b0660 0.28 -1.4 692,300 - ybeZ b0660 0.71 -0.4 692,300 - ybeZ b0660 0.71 -1.9 692,407 - ybeZ b0660 0.81 -1.4 692,407 - ybeZ b0660 0.81 +1.0 692,576 - -0.5 692,576 - -0.6 692,598 - +0.3 692,598 - -0.4 692,774 + +0.6 692,914 + miaB b0661 0.11 +0.1 693,086 + miaB b0661 0.23 +1.0 693,088 + miaB b0661 0.23 +0.1 693,088 + miaB b0661 0.23 -0.6 693,141 + miaB b0661 0.27 -0.8 693,175 + miaB b0661 0.30 +0.1 693,175 - miaB b0661 0.30 +0.4 693,175 - miaB b0661 0.30 +1.3 693,183 - miaB b0661 0.30 +0.4 693,183 - miaB b0661 0.30 +0.3 693,236 - miaB b0661 0.34 +0.3 693,302 + miaB b0661 0.38 -1.2 693,310 - miaB b0661 0.39 +1.6 693,310 - miaB b0661 0.39 +0.2 693,310 - miaB b0661 0.39 +1.7 693,310 - miaB b0661 0.39 -0.1 693,337 - miaB b0661 0.41 +0.2 693,380 + miaB b0661 0.44 -0.6 693,380 + miaB b0661 0.44 -0.3 693,380 + miaB b0661 0.44 +0.5 693,595 - miaB b0661 0.59 +0.6
Or see this region's nucleotide sequence