Experiment: LB, Anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt aspS and yecD are separated by 309 nucleotides yecD and yecE overlap by 4 nucleotides yecE and yecN are separated by 52 nucleotides
b1866: aspS - aspartyl-tRNA synthetase (NCBI), at 1,946,774 to 1,948,546
aspS
b1867: yecD - orf, hypothetical protein (VIMSS), at 1,948,856 to 1,949,422
yecD
b1868: yecE - hypothetical protein (NCBI), at 1,949,419 to 1,950,237
yecE
b1869: yecN - orf, hypothetical protein (VIMSS), at 1,950,290 to 1,950,685
yecN
Position (kb)
1948
1949
1950 Strain fitness (log2 ratio)
-1
0
1
2
3 at 1948.556 kb on - strand at 1948.718 kb on - strand at 1948.761 kb on + strand at 1948.771 kb on + strand at 1948.859 kb on - strand at 1948.859 kb on - strand at 1948.932 kb on + strand, within yecD at 1949.048 kb on + strand, within yecD at 1949.173 kb on + strand, within yecD at 1949.173 kb on + strand, within yecD at 1949.179 kb on - strand, within yecD at 1949.186 kb on + strand, within yecD at 1949.186 kb on + strand, within yecD at 1949.189 kb on - strand, within yecD at 1949.228 kb on + strand, within yecD at 1949.228 kb on + strand, within yecD at 1949.228 kb on - strand, within yecD at 1949.228 kb on - strand, within yecD at 1949.231 kb on - strand, within yecD at 1949.349 kb on + strand, within yecD at 1949.404 kb on - strand at 1949.404 kb on - strand at 1949.442 kb on - strand at 1949.709 kb on + strand, within yecE at 1949.709 kb on + strand, within yecE at 1949.746 kb on - strand, within yecE at 1949.842 kb on - strand, within yecE at 1949.842 kb on - strand, within yecE at 1949.931 kb on + strand, within yecE at 1950.249 kb on + strand at 1950.310 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB, Anaerobic remove 1,948,556 - +3.7 1,948,718 - -0.8 1,948,761 + -0.3 1,948,771 + +0.3 1,948,859 - -1.3 1,948,859 - -0.3 1,948,932 + yecD b1867 0.13 -0.1 1,949,048 + yecD b1867 0.34 +0.9 1,949,173 + yecD b1867 0.56 +0.5 1,949,173 + yecD b1867 0.56 -0.3 1,949,179 - yecD b1867 0.57 +0.1 1,949,186 + yecD b1867 0.58 +0.3 1,949,186 + yecD b1867 0.58 -0.9 1,949,189 - yecD b1867 0.59 +0.8 1,949,228 + yecD b1867 0.66 +0.4 1,949,228 + yecD b1867 0.66 +1.3 1,949,228 - yecD b1867 0.66 +1.0 1,949,228 - yecD b1867 0.66 +0.7 1,949,231 - yecD b1867 0.66 -0.5 1,949,349 + yecD b1867 0.87 +0.1 1,949,404 - -0.9 1,949,404 - -1.4 1,949,442 - -0.8 1,949,709 + yecE b1868 0.35 +1.3 1,949,709 + yecE b1868 0.35 +1.0 1,949,746 - yecE b1868 0.40 +0.4 1,949,842 - yecE b1868 0.52 -0.0 1,949,842 - yecE b1868 0.52 -0.9 1,949,931 + yecE b1868 0.63 +0.2 1,950,249 + -0.4 1,950,310 - -0.8
Or see this region's nucleotide sequence