Experiment: LB, Anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydiR and ydiS are separated by 55 nucleotides ydiS and ydiT overlap by 4 nucleotides ydiT and fadK are separated by 56 nucleotides
b1698: ydiR - predicted electron transfer flavoprotein, FAD-binding (NCBI), at 1,778,425 to 1,779,363
ydiR
b1699: ydiS - predicted oxidoreductase with FAD/NAD(P)-binding domain (NCBI), at 1,779,419 to 1,780,708
ydiS
b1700: ydiT - predicted 4Fe-4S ferredoxin-type protein (NCBI), at 1,780,705 to 1,780,998
ydiT
b1701: fadK - short chain acyl-CoA synthetase, anaerobic (RefSeq), at 1,781,055 to 1,782,701
fadK
Position (kb)
1779
1780
1781 Strain fitness (log2 ratio)
-1
0
1 at 1778.469 kb on + strand at 1778.740 kb on + strand, within ydiR at 1778.933 kb on - strand, within ydiR at 1779.145 kb on + strand, within ydiR at 1779.222 kb on - strand, within ydiR at 1779.423 kb on - strand at 1779.424 kb on - strand at 1779.424 kb on - strand at 1779.484 kb on + strand at 1779.721 kb on + strand, within ydiS at 1779.847 kb on + strand, within ydiS at 1780.127 kb on - strand, within ydiS at 1780.378 kb on - strand, within ydiS at 1780.483 kb on - strand, within ydiS at 1780.483 kb on - strand, within ydiS at 1780.566 kb on - strand, within ydiS at 1780.580 kb on + strand at 1780.598 kb on + strand at 1780.939 kb on + strand, within ydiT at 1781.119 kb on - strand at 1781.209 kb on - strand at 1781.217 kb on - strand at 1781.266 kb on + strand, within fadK at 1781.399 kb on - strand, within fadK at 1781.530 kb on + strand, within fadK
Per-strain Table
Position Strand Gene LocusTag Fraction LB, Anaerobic remove 1,778,469 + -0.1 1,778,740 + ydiR b1698 0.34 -1.2 1,778,933 - ydiR b1698 0.54 +0.5 1,779,145 + ydiR b1698 0.77 +1.2 1,779,222 - ydiR b1698 0.85 +0.5 1,779,423 - -0.3 1,779,424 - -0.6 1,779,424 - -0.5 1,779,484 + -1.0 1,779,721 + ydiS b1699 0.23 +0.6 1,779,847 + ydiS b1699 0.33 -0.0 1,780,127 - ydiS b1699 0.55 -0.3 1,780,378 - ydiS b1699 0.74 +1.2 1,780,483 - ydiS b1699 0.82 +1.5 1,780,483 - ydiS b1699 0.82 +0.7 1,780,566 - ydiS b1699 0.89 +0.4 1,780,580 + -0.1 1,780,598 + +0.2 1,780,939 + ydiT b1700 0.80 -1.6 1,781,119 - +0.2 1,781,209 - -0.0 1,781,217 - +0.6 1,781,266 + fadK b1701 0.13 -0.3 1,781,399 - fadK b1701 0.21 +0.9 1,781,530 + fadK b1701 0.29 +0.4
Or see this region's nucleotide sequence