Experiment: LB, Anaerobic
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt agp and yccJ are separated by 37 nucleotides yccJ and wrbA are separated by 20 nucleotides wrbA and ycdF are separated by 372 nucleotides ycdF and ycdG are separated by 256 nucleotides
b1002: agp - glucose-1-phosphatase/inositol phosphatase (NCBI), at 1,064,808 to 1,066,049
agp
b1003: yccJ - hypothetical protein (NCBI), at 1,066,087 to 1,066,314
yccJ
b1004: wrbA - TrpR binding protein WrbA (NCBI), at 1,066,335 to 1,066,931
wrbA
b4518: ycdF - orf, hypothetical protein (VIMSS), at 1,067,304 to 1,067,477
ycdF
b1006: ycdG - putative transport protein (VIMSS), at 1,067,734 to 1,069,062
ycdG
Position (kb)
1066
1067 Strain fitness (log2 ratio)
-1
0
1
2 at 1065.339 kb on + strand, within agp at 1065.578 kb on + strand, within agp at 1065.620 kb on - strand, within agp at 1065.634 kb on - strand, within agp at 1065.634 kb on - strand, within agp at 1065.655 kb on - strand, within agp at 1065.692 kb on + strand, within agp at 1065.699 kb on + strand, within agp at 1065.750 kb on - strand, within agp at 1065.750 kb on - strand, within agp at 1065.803 kb on + strand, within agp at 1065.846 kb on + strand, within agp at 1065.899 kb on - strand, within agp at 1065.901 kb on + strand, within agp at 1065.901 kb on + strand, within agp at 1065.936 kb on - strand at 1065.983 kb on + strand at 1066.079 kb on + strand at 1066.415 kb on + strand, within wrbA at 1066.460 kb on - strand, within wrbA at 1066.550 kb on + strand, within wrbA at 1066.550 kb on + strand, within wrbA at 1066.562 kb on - strand, within wrbA at 1066.562 kb on - strand, within wrbA at 1066.563 kb on - strand, within wrbA at 1066.630 kb on + strand, within wrbA at 1066.651 kb on + strand, within wrbA at 1066.821 kb on - strand, within wrbA at 1066.849 kb on - strand, within wrbA at 1066.911 kb on - strand at 1066.947 kb on + strand at 1066.951 kb on - strand at 1067.176 kb on - strand at 1067.203 kb on - strand at 1067.203 kb on - strand at 1067.205 kb on + strand at 1067.212 kb on - strand at 1067.214 kb on + strand at 1067.214 kb on + strand at 1067.214 kb on + strand at 1067.214 kb on + strand at 1067.254 kb on - strand at 1067.254 kb on - strand at 1067.254 kb on - strand at 1067.254 kb on - strand at 1067.254 kb on - strand at 1067.279 kb on + strand at 1067.302 kb on - strand at 1067.589 kb on + strand at 1067.833 kb on - strand at 1067.883 kb on + strand, within ycdG at 1067.922 kb on + strand, within ycdG
Per-strain Table
Position Strand Gene LocusTag Fraction LB, Anaerobic remove 1,065,339 + agp b1002 0.43 -0.1 1,065,578 + agp b1002 0.62 +0.6 1,065,620 - agp b1002 0.65 -1.6 1,065,634 - agp b1002 0.67 -0.2 1,065,634 - agp b1002 0.67 -0.0 1,065,655 - agp b1002 0.68 +0.3 1,065,692 + agp b1002 0.71 -1.3 1,065,699 + agp b1002 0.72 +0.2 1,065,750 - agp b1002 0.76 -0.2 1,065,750 - agp b1002 0.76 -0.7 1,065,803 + agp b1002 0.80 +0.9 1,065,846 + agp b1002 0.84 -1.2 1,065,899 - agp b1002 0.88 -0.7 1,065,901 + agp b1002 0.88 +0.7 1,065,901 + agp b1002 0.88 +0.3 1,065,936 - +0.1 1,065,983 + +0.2 1,066,079 + +1.5 1,066,415 + wrbA b1004 0.13 -0.8 1,066,460 - wrbA b1004 0.21 +0.4 1,066,550 + wrbA b1004 0.36 -0.9 1,066,550 + wrbA b1004 0.36 +0.8 1,066,562 - wrbA b1004 0.38 +0.8 1,066,562 - wrbA b1004 0.38 +0.8 1,066,563 - wrbA b1004 0.38 -0.5 1,066,630 + wrbA b1004 0.49 +1.1 1,066,651 + wrbA b1004 0.53 +0.4 1,066,821 - wrbA b1004 0.81 +0.4 1,066,849 - wrbA b1004 0.86 +1.5 1,066,911 - -1.0 1,066,947 + -0.8 1,066,951 - +0.3 1,067,176 - +1.8 1,067,203 - +0.5 1,067,203 - -0.5 1,067,205 + -0.2 1,067,212 - +0.5 1,067,214 + -0.1 1,067,214 + +0.4 1,067,214 + -0.3 1,067,214 + +0.0 1,067,254 - +1.8 1,067,254 - -0.4 1,067,254 - -0.0 1,067,254 - -0.1 1,067,254 - +1.2 1,067,279 + -0.6 1,067,302 - -0.3 1,067,589 + +0.4 1,067,833 - -0.2 1,067,883 + ycdG b1006 0.11 -0.5 1,067,922 + ycdG b1006 0.14 -0.4
Or see this region's nucleotide sequence