Experiment: LB Anaerobic with Norfloxacin 0.00000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ymfL and ymfM overlap by 4 nucleotides ymfM and oweE are separated by 9 nucleotides oweE and aaaE are separated by 0 nucleotides aaaE and ymfR are separated by 11 nucleotides ymfR and ymfO overlap by 1 nucleotides ymfO and ymfP are separated by 0 nucleotides
b1147: ymfL - e14 prophage; predicted DNA-binding transcriptional regulator (RefSeq), at 1,202,491 to 1,203,048
ymfL
b1148: ymfM - e14 prophage; predicted protein (NCBI), at 1,203,045 to 1,203,383
ymfM
b4692: oweE - no description, at 1,203,393 to 1,203,626
oweE
b4693: aaaE - no description, at 1,203,627 to 1,204,760
aaaE
b1150: ymfR - e14 prophage; predicted protein (NCBI), at 1,204,772 to 1,204,954
ymfR
b1151: ymfO - e14 prophage; conserved protein (RefSeq), at 1,204,954 to 1,205,365
ymfO
b1152: ymfP - orf, hypothetical protein (VIMSS), at 1,205,366 to 1,206,145
ymfP
Position (kb)
1203
1204
1205 Strain fitness (log2 ratio)
-1
0
1
2 at 1202.825 kb on - strand, within ymfL at 1202.869 kb on - strand, within ymfL at 1202.869 kb on - strand, within ymfL at 1202.897 kb on - strand, within ymfL at 1202.897 kb on - strand, within ymfL at 1202.909 kb on - strand, within ymfL at 1203.060 kb on + strand at 1203.060 kb on + strand at 1203.173 kb on - strand, within ymfM at 1203.180 kb on - strand, within ymfM at 1203.189 kb on - strand, within ymfM at 1203.189 kb on - strand, within ymfM at 1203.378 kb on + strand at 1203.378 kb on + strand at 1203.435 kb on + strand, within oweE at 1203.490 kb on + strand, within oweE at 1203.556 kb on + strand, within oweE at 1203.617 kb on + strand at 1203.617 kb on + strand at 1203.646 kb on - strand at 1203.684 kb on - strand at 1203.684 kb on - strand at 1203.872 kb on + strand, within aaaE at 1203.872 kb on + strand, within aaaE at 1203.872 kb on + strand, within aaaE at 1203.872 kb on + strand, within aaaE at 1203.880 kb on - strand, within aaaE at 1203.891 kb on + strand, within aaaE at 1204.010 kb on + strand, within aaaE at 1204.018 kb on - strand, within aaaE at 1204.078 kb on - strand, within aaaE at 1204.084 kb on + strand, within aaaE at 1204.196 kb on + strand, within aaaE at 1204.204 kb on - strand, within aaaE at 1204.237 kb on - strand, within aaaE at 1204.391 kb on - strand, within aaaE at 1204.420 kb on - strand, within aaaE at 1204.462 kb on + strand, within aaaE at 1204.841 kb on - strand, within ymfR at 1204.996 kb on + strand, within ymfO at 1205.042 kb on - strand, within ymfO at 1205.128 kb on + strand, within ymfO at 1205.226 kb on - strand, within ymfO at 1205.226 kb on - strand, within ymfO at 1205.409 kb on + strand at 1205.409 kb on + strand at 1205.435 kb on - strand at 1205.437 kb on - strand at 1205.474 kb on + strand, within ymfP at 1205.474 kb on + strand, within ymfP at 1205.630 kb on + strand, within ymfP at 1205.661 kb on - strand, within ymfP at 1205.709 kb on - strand, within ymfP at 1205.740 kb on - strand, within ymfP
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Norfloxacin 0.00000015625 mM remove 1,202,825 - ymfL b1147 0.60 +0.4 1,202,869 - ymfL b1147 0.68 +1.7 1,202,869 - ymfL b1147 0.68 +0.2 1,202,897 - ymfL b1147 0.73 +0.1 1,202,897 - ymfL b1147 0.73 +2.1 1,202,909 - ymfL b1147 0.75 +0.7 1,203,060 + +1.7 1,203,060 + +0.7 1,203,173 - ymfM b1148 0.38 +1.3 1,203,180 - ymfM b1148 0.40 +1.7 1,203,189 - ymfM b1148 0.42 +1.0 1,203,189 - ymfM b1148 0.42 +1.1 1,203,378 + +1.5 1,203,378 + -0.2 1,203,435 + oweE b4692 0.18 +1.1 1,203,490 + oweE b4692 0.41 +1.1 1,203,556 + oweE b4692 0.70 +0.5 1,203,617 + +0.2 1,203,617 + +0.4 1,203,646 - +0.8 1,203,684 - +0.1 1,203,684 - +1.7 1,203,872 + aaaE b4693 0.22 +0.6 1,203,872 + aaaE b4693 0.22 +2.5 1,203,872 + aaaE b4693 0.22 +1.5 1,203,872 + aaaE b4693 0.22 +1.7 1,203,880 - aaaE b4693 0.22 +0.8 1,203,891 + aaaE b4693 0.23 +0.2 1,204,010 + aaaE b4693 0.34 +1.1 1,204,018 - aaaE b4693 0.34 +1.6 1,204,078 - aaaE b4693 0.40 +1.5 1,204,084 + aaaE b4693 0.40 +0.5 1,204,196 + aaaE b4693 0.50 +0.8 1,204,204 - aaaE b4693 0.51 +1.0 1,204,237 - aaaE b4693 0.54 +2.0 1,204,391 - aaaE b4693 0.67 +0.7 1,204,420 - aaaE b4693 0.70 +1.5 1,204,462 + aaaE b4693 0.74 +0.1 1,204,841 - ymfR b1150 0.38 -1.3 1,204,996 + ymfO b1151 0.10 -0.3 1,205,042 - ymfO b1151 0.21 +0.4 1,205,128 + ymfO b1151 0.42 -1.0 1,205,226 - ymfO b1151 0.66 +2.4 1,205,226 - ymfO b1151 0.66 +1.7 1,205,409 + +0.9 1,205,409 + +1.0 1,205,435 - -0.8 1,205,437 - +0.9 1,205,474 + ymfP b1152 0.14 +0.4 1,205,474 + ymfP b1152 0.14 +1.3 1,205,630 + ymfP b1152 0.34 +0.9 1,205,661 - ymfP b1152 0.38 +0.5 1,205,709 - ymfP b1152 0.44 +1.9 1,205,740 - ymfP b1152 0.48 +0.8
Or see this region's nucleotide sequence