Strain Fitness in Escherichia coli BW25113 around b1158

Experiment: LB Anaerobic with Norfloxacin 0.00000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttfaE and stfE overlap by 1 nucleotidesstfE and pin are separated by 65 nucleotidespin and mcrA are separated by 106 nucleotides b1156: tfaE - e14 prophage; predicted tail fiber assembly protein (NCBI), at 1,207,740 to 1,208,342 tfaE b1157: stfE - no description, at 1,208,342 to 1,208,842 stfE b1158: pin - e14 prophage; site-specific DNA recombinase (NCBI), at 1,208,908 to 1,209,462 pin b1159: mcrA - e14 prophage; 5-methylcytosine-specific restriction endonuclease B (NCBI), at 1,209,569 to 1,210,402 mcrA Position (kb) 1208 1209 1210Strain fitness (log2 ratio) -1 0 1 2 3at 1207.959 kb on + strand, within tfaEat 1207.962 kb on - strand, within tfaEat 1207.964 kb on - strand, within tfaEat 1207.967 kb on + strand, within tfaEat 1207.972 kb on + strand, within tfaEat 1208.100 kb on - strand, within tfaEat 1208.167 kb on - strand, within tfaEat 1208.167 kb on - strand, within tfaEat 1208.168 kb on - strand, within tfaEat 1208.168 kb on - strand, within tfaEat 1208.170 kb on + strand, within tfaEat 1208.170 kb on + strand, within tfaEat 1208.172 kb on + strand, within tfaEat 1208.177 kb on + strand, within tfaEat 1208.178 kb on - strand, within tfaEat 1208.178 kb on - strand, within tfaEat 1208.180 kb on - strand, within tfaEat 1208.180 kb on - strand, within tfaEat 1208.184 kb on + strand, within tfaEat 1208.185 kb on - strand, within tfaEat 1208.187 kb on - strand, within tfaEat 1208.194 kb on + strand, within tfaEat 1208.194 kb on + strand, within tfaEat 1208.194 kb on + strand, within tfaEat 1208.352 kb on - strandat 1208.413 kb on - strand, within stfEat 1208.413 kb on - strand, within stfEat 1208.441 kb on - strand, within stfEat 1208.560 kb on - strand, within stfEat 1208.560 kb on - strand, within stfEat 1208.575 kb on - strand, within stfEat 1208.576 kb on - strand, within stfEat 1208.586 kb on - strand, within stfEat 1208.604 kb on - strand, within stfEat 1208.604 kb on - strand, within stfEat 1208.604 kb on - strand, within stfEat 1208.606 kb on - strand, within stfEat 1208.609 kb on - strand, within stfEat 1208.638 kb on - strand, within stfEat 1208.642 kb on - strand, within stfEat 1208.642 kb on - strand, within stfEat 1208.642 kb on - strand, within stfEat 1208.648 kb on + strand, within stfEat 1208.752 kb on + strand, within stfEat 1208.756 kb on - strand, within stfEat 1208.791 kb on - strand, within stfEat 1208.819 kb on + strandat 1208.838 kb on - strandat 1208.899 kb on - strandat 1208.946 kb on + strandat 1208.946 kb on + strandat 1208.964 kb on - strand, within pinat 1208.964 kb on - strand, within pinat 1208.968 kb on + strand, within pinat 1209.015 kb on - strand, within pinat 1209.110 kb on - strand, within pinat 1209.296 kb on + strand, within pinat 1209.296 kb on + strand, within pinat 1209.306 kb on + strand, within pinat 1209.306 kb on + strandat 1209.316 kb on + strand, within pinat 1209.316 kb on + strand, within pinat 1209.333 kb on + strand, within pinat 1209.348 kb on + strand, within pinat 1209.397 kb on + strand, within pinat 1209.408 kb on + strandat 1209.423 kb on + strandat 1209.612 kb on + strandat 1209.612 kb on + strandat 1209.620 kb on + strandat 1209.635 kb on + strandat 1209.648 kb on + strandat 1209.648 kb on + strandat 1209.663 kb on + strand, within mcrAat 1209.743 kb on + strand, within mcrAat 1209.769 kb on + strand, within mcrAat 1210.309 kb on + strand, within mcrAat 1210.442 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Norfloxacin 0.00000015625 mM
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1,207,959 + tfaE b1156 0.36 +0.1
1,207,962 - tfaE b1156 0.37 +0.3
1,207,964 - tfaE b1156 0.37 +0.1
1,207,967 + tfaE b1156 0.38 -0.3
1,207,972 + tfaE b1156 0.38 +0.7
1,208,100 - tfaE b1156 0.60 +1.1
1,208,167 - tfaE b1156 0.71 -0.1
1,208,167 - tfaE b1156 0.71 -0.2
1,208,168 - tfaE b1156 0.71 +1.7
1,208,168 - tfaE b1156 0.71 +1.7
1,208,170 + tfaE b1156 0.71 +1.7
1,208,170 + tfaE b1156 0.71 +0.7
1,208,172 + tfaE b1156 0.72 +1.7
1,208,177 + tfaE b1156 0.72 +1.7
1,208,178 - tfaE b1156 0.73 +3.2
1,208,178 - tfaE b1156 0.73 -0.7
1,208,180 - tfaE b1156 0.73 +2.2
1,208,180 - tfaE b1156 0.73 +1.7
1,208,184 + tfaE b1156 0.74 +1.7
1,208,185 - tfaE b1156 0.74 +0.1
1,208,187 - tfaE b1156 0.74 +0.7
1,208,194 + tfaE b1156 0.75 +1.7
1,208,194 + tfaE b1156 0.75 +1.7
1,208,194 + tfaE b1156 0.75 +1.7
1,208,352 - -0.3
1,208,413 - stfE b1157 0.14 +1.7
1,208,413 - stfE b1157 0.14 -0.3
1,208,441 - stfE b1157 0.20 +2.0
1,208,560 - stfE b1157 0.44 +0.2
1,208,560 - stfE b1157 0.44 +0.8
1,208,575 - stfE b1157 0.47 +0.4
1,208,576 - stfE b1157 0.47 -0.7
1,208,586 - stfE b1157 0.49 +1.5
1,208,604 - stfE b1157 0.52 +1.6
1,208,604 - stfE b1157 0.52 +2.9
1,208,604 - stfE b1157 0.52 +1.0
1,208,606 - stfE b1157 0.53 -0.8
1,208,609 - stfE b1157 0.53 -1.4
1,208,638 - stfE b1157 0.59 +0.6
1,208,642 - stfE b1157 0.60 +1.0
1,208,642 - stfE b1157 0.60 +1.7
1,208,642 - stfE b1157 0.60 +3.4
1,208,648 + stfE b1157 0.61 +1.7
1,208,752 + stfE b1157 0.82 +2.3
1,208,756 - stfE b1157 0.83 +0.5
1,208,791 - stfE b1157 0.90 -0.7
1,208,819 + +2.3
1,208,838 - +2.2
1,208,899 - -1.0
1,208,946 + +2.1
1,208,946 + +0.9
1,208,964 - pin b1158 0.10 +2.2
1,208,964 - pin b1158 0.10 -0.2
1,208,968 + pin b1158 0.11 +0.7
1,209,015 - pin b1158 0.19 +1.6
1,209,110 - pin b1158 0.36 +1.7
1,209,296 + pin b1158 0.70 -0.6
1,209,296 + pin b1158 0.70 -1.2
1,209,306 + pin b1158 0.72 +1.0
1,209,306 + +2.2
1,209,316 + pin b1158 0.74 +0.3
1,209,316 + pin b1158 0.74 +1.0
1,209,333 + pin b1158 0.77 +0.1
1,209,348 + pin b1158 0.79 +0.4
1,209,397 + pin b1158 0.88 +0.4
1,209,408 + +0.2
1,209,423 + +0.4
1,209,612 + -0.9
1,209,612 + +0.9
1,209,620 + +0.2
1,209,635 + +0.1
1,209,648 + +1.9
1,209,648 + -1.3
1,209,663 + mcrA b1159 0.11 +0.7
1,209,743 + mcrA b1159 0.21 +1.7
1,209,769 + mcrA b1159 0.24 +0.1
1,210,309 + mcrA b1159 0.89 +1.7
1,210,442 - +1.7

Or see this region's nucleotide sequence