Experiment: LB Anaerobic with Ramoplanin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhgF and feoA are separated by 456 nucleotides feoA and feoB are separated by 16 nucleotides
b3407: yhgF - orf, hypothetical protein (VIMSS), at 3,535,407 to 3,537,728
yhgF
b3408: feoA - ferrous iron transport protein A (NCBI), at 3,538,185 to 3,538,412
feoA
b3409: feoB - ferrous iron transport protein B (NCBI), at 3,538,429 to 3,540,750
feoB
Position (kb)
3538
3539 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3537.187 kb on + strand, within yhgF at 3537.187 kb on + strand, within yhgF at 3537.187 kb on - strand, within yhgF at 3537.187 kb on - strand, within yhgF at 3537.188 kb on - strand, within yhgF at 3537.202 kb on + strand, within yhgF at 3537.202 kb on + strand, within yhgF at 3537.225 kb on + strand, within yhgF at 3537.260 kb on + strand, within yhgF at 3537.260 kb on + strand, within yhgF at 3537.268 kb on - strand, within yhgF at 3537.346 kb on + strand, within yhgF at 3537.346 kb on + strand, within yhgF at 3537.423 kb on + strand, within yhgF at 3537.423 kb on + strand, within yhgF at 3537.423 kb on + strand, within yhgF at 3537.431 kb on - strand, within yhgF at 3537.446 kb on - strand, within yhgF at 3537.482 kb on - strand, within yhgF at 3537.560 kb on - strand at 3537.566 kb on - strand at 3537.655 kb on + strand at 3537.663 kb on - strand at 3537.663 kb on - strand at 3537.663 kb on - strand at 3537.693 kb on + strand at 3537.695 kb on - strand at 3537.730 kb on + strand at 3537.735 kb on + strand at 3537.738 kb on - strand at 3537.740 kb on + strand at 3537.740 kb on + strand at 3537.763 kb on + strand at 3537.766 kb on + strand at 3537.774 kb on - strand at 3537.776 kb on + strand at 3537.784 kb on + strand at 3537.784 kb on - strand at 3537.784 kb on - strand at 3537.784 kb on - strand at 3537.784 kb on - strand at 3537.785 kb on + strand at 3537.785 kb on + strand at 3537.796 kb on + strand at 3537.810 kb on + strand at 3537.816 kb on - strand at 3537.819 kb on + strand at 3537.819 kb on + strand at 3537.819 kb on + strand at 3537.827 kb on - strand at 3537.827 kb on - strand at 3537.868 kb on - strand at 3537.868 kb on - strand at 3537.868 kb on - strand at 3537.868 kb on - strand at 3537.934 kb on + strand at 3537.943 kb on - strand at 3537.943 kb on - strand at 3537.964 kb on - strand at 3537.964 kb on - strand at 3537.987 kb on - strand at 3537.987 kb on - strand at 3537.987 kb on - strand at 3537.990 kb on + strand at 3538.000 kb on + strand at 3538.000 kb on + strand at 3538.034 kb on + strand at 3538.036 kb on + strand at 3538.036 kb on + strand at 3538.042 kb on - strand at 3538.044 kb on - strand at 3538.044 kb on - strand at 3538.080 kb on + strand at 3538.080 kb on + strand at 3538.111 kb on + strand at 3538.119 kb on - strand at 3538.123 kb on + strand at 3538.140 kb on + strand at 3538.140 kb on + strand at 3538.142 kb on + strand at 3538.142 kb on + strand at 3538.150 kb on + strand at 3538.155 kb on - strand at 3538.163 kb on + strand at 3538.172 kb on + strand at 3538.172 kb on + strand at 3538.172 kb on - strand at 3538.185 kb on + strand at 3538.185 kb on + strand at 3538.193 kb on - strand at 3538.193 kb on - strand at 3538.193 kb on - strand at 3538.195 kb on + strand at 3538.204 kb on - strand at 3538.204 kb on - strand at 3538.219 kb on - strand, within feoA at 3538.240 kb on + strand, within feoA at 3538.240 kb on + strand, within feoA at 3538.267 kb on + strand, within feoA at 3538.273 kb on + strand, within feoA at 3538.273 kb on + strand, within feoA at 3538.275 kb on - strand, within feoA at 3538.275 kb on - strand, within feoA at 3538.277 kb on + strand, within feoA at 3538.277 kb on + strand, within feoA at 3538.279 kb on + strand, within feoA at 3538.291 kb on + strand, within feoA at 3538.297 kb on + strand, within feoA at 3538.299 kb on + strand, within feoA at 3538.299 kb on - strand, within feoA at 3538.301 kb on + strand, within feoA at 3538.301 kb on + strand, within feoA at 3538.301 kb on + strand, within feoA at 3538.306 kb on + strand, within feoA at 3538.307 kb on - strand, within feoA at 3538.309 kb on + strand, within feoA at 3538.311 kb on + strand, within feoA at 3538.311 kb on + strand, within feoA at 3538.319 kb on + strand, within feoA at 3538.319 kb on - strand, within feoA at 3538.319 kb on - strand, within feoA at 3538.319 kb on - strand, within feoA at 3538.319 kb on - strand, within feoA at 3538.319 kb on - strand, within feoA at 3538.324 kb on + strand, within feoA at 3538.332 kb on - strand, within feoA at 3538.335 kb on + strand, within feoA at 3538.335 kb on + strand, within feoA at 3538.336 kb on - strand, within feoA at 3538.336 kb on - strand at 3538.336 kb on - strand, within feoA at 3538.339 kb on + strand, within feoA at 3538.342 kb on + strand, within feoA at 3538.342 kb on + strand, within feoA at 3538.342 kb on - strand, within feoA at 3538.344 kb on - strand, within feoA at 3538.348 kb on + strand, within feoA at 3538.348 kb on + strand, within feoA at 3538.352 kb on - strand, within feoA at 3538.352 kb on - strand, within feoA at 3538.353 kb on + strand, within feoA at 3538.353 kb on + strand, within feoA at 3538.359 kb on + strand, within feoA at 3538.361 kb on - strand, within feoA at 3538.362 kb on - strand, within feoA at 3538.362 kb on - strand, within feoA at 3538.362 kb on - strand, within feoA at 3538.362 kb on - strand, within feoA at 3538.384 kb on + strand, within feoA at 3538.384 kb on + strand, within feoA at 3538.392 kb on - strand at 3538.392 kb on - strand at 3538.417 kb on - strand at 3538.417 kb on - strand at 3538.455 kb on - strand at 3538.494 kb on + strand at 3538.494 kb on + strand at 3538.494 kb on + strand at 3538.501 kb on + strand at 3538.501 kb on + strand at 3538.507 kb on + strand at 3538.511 kb on + strand at 3538.512 kb on + strand at 3538.512 kb on + strand at 3538.515 kb on - strand at 3538.516 kb on + strand at 3538.516 kb on + strand at 3538.517 kb on - strand at 3538.517 kb on - strand at 3538.518 kb on + strand at 3538.524 kb on - strand at 3538.526 kb on - strand at 3538.532 kb on + strand at 3538.532 kb on + strand at 3538.534 kb on - strand at 3538.539 kb on - strand at 3538.540 kb on - strand at 3538.540 kb on - strand at 3538.540 kb on - strand at 3538.540 kb on - strand at 3538.546 kb on + strand at 3538.563 kb on + strand at 3538.563 kb on + strand at 3538.563 kb on + strand at 3538.568 kb on - strand at 3538.571 kb on - strand at 3538.586 kb on + strand at 3538.586 kb on + strand at 3538.586 kb on + strand at 3538.588 kb on + strand at 3538.589 kb on - strand at 3538.593 kb on - strand at 3538.596 kb on - strand at 3538.597 kb on - strand at 3538.597 kb on - strand at 3538.607 kb on - strand at 3538.615 kb on - strand at 3538.633 kb on - strand at 3538.633 kb on - strand at 3538.635 kb on + strand at 3538.650 kb on - strand at 3538.665 kb on - strand, within feoB at 3538.665 kb on - strand, within feoB at 3538.669 kb on + strand at 3538.669 kb on + strand, within feoB at 3538.673 kb on - strand, within feoB at 3538.712 kb on + strand, within feoB at 3538.719 kb on + strand, within feoB at 3538.738 kb on - strand, within feoB at 3538.738 kb on - strand, within feoB at 3538.744 kb on + strand, within feoB at 3538.750 kb on + strand, within feoB at 3538.750 kb on + strand, within feoB at 3538.767 kb on + strand, within feoB at 3538.775 kb on - strand, within feoB at 3538.783 kb on - strand, within feoB at 3538.793 kb on - strand, within feoB at 3538.793 kb on - strand, within feoB at 3538.807 kb on - strand, within feoB at 3538.807 kb on - strand, within feoB at 3538.846 kb on + strand, within feoB at 3538.846 kb on + strand, within feoB at 3538.846 kb on + strand, within feoB at 3538.854 kb on - strand, within feoB at 3538.854 kb on - strand, within feoB at 3538.854 kb on - strand, within feoB at 3538.923 kb on + strand, within feoB at 3538.946 kb on - strand, within feoB at 3538.955 kb on + strand, within feoB at 3538.965 kb on + strand, within feoB at 3539.031 kb on + strand, within feoB at 3539.094 kb on + strand, within feoB at 3539.149 kb on + strand, within feoB at 3539.149 kb on + strand, within feoB at 3539.157 kb on - strand, within feoB at 3539.157 kb on - strand, within feoB at 3539.176 kb on - strand, within feoB at 3539.186 kb on - strand, within feoB at 3539.186 kb on - strand, within feoB at 3539.205 kb on + strand, within feoB at 3539.214 kb on + strand, within feoB at 3539.217 kb on + strand, within feoB at 3539.217 kb on + strand, within feoB at 3539.227 kb on - strand, within feoB at 3539.258 kb on - strand, within feoB at 3539.260 kb on - strand, within feoB at 3539.260 kb on - strand, within feoB at 3539.296 kb on + strand, within feoB at 3539.343 kb on + strand, within feoB at 3539.353 kb on + strand, within feoB at 3539.394 kb on + strand, within feoB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Ramoplanin 0.00003125 mM remove 3,537,187 + yhgF b3407 0.77 +1.0 3,537,187 + yhgF b3407 0.77 -0.2 3,537,187 - yhgF b3407 0.77 +0.8 3,537,187 - yhgF b3407 0.77 -0.5 3,537,188 - yhgF b3407 0.77 +0.6 3,537,202 + yhgF b3407 0.77 +0.1 3,537,202 + yhgF b3407 0.77 -0.2 3,537,225 + yhgF b3407 0.78 +0.1 3,537,260 + yhgF b3407 0.80 +1.3 3,537,260 + yhgF b3407 0.80 +1.1 3,537,268 - yhgF b3407 0.80 -0.3 3,537,346 + yhgF b3407 0.84 +0.6 3,537,346 + yhgF b3407 0.84 +1.1 3,537,423 + yhgF b3407 0.87 -0.4 3,537,423 + yhgF b3407 0.87 +0.4 3,537,423 + yhgF b3407 0.87 +2.0 3,537,431 - yhgF b3407 0.87 -0.7 3,537,446 - yhgF b3407 0.88 -1.7 3,537,482 - yhgF b3407 0.89 -0.3 3,537,560 - +0.7 3,537,566 - -1.1 3,537,655 + +0.4 3,537,663 - -0.6 3,537,663 - -2.6 3,537,663 - -2.0 3,537,693 + +0.3 3,537,695 - -0.6 3,537,730 + +0.3 3,537,735 + +0.0 3,537,738 - +0.4 3,537,740 + -0.6 3,537,740 + +0.7 3,537,763 + +0.6 3,537,766 + -0.4 3,537,774 - +0.4 3,537,776 + -0.8 3,537,784 + -0.3 3,537,784 - +0.4 3,537,784 - -0.2 3,537,784 - +0.4 3,537,784 - -1.0 3,537,785 + +1.4 3,537,785 + -0.3 3,537,796 + +0.3 3,537,810 + +0.9 3,537,816 - +0.0 3,537,819 + -1.3 3,537,819 + -0.2 3,537,819 + +0.1 3,537,827 - +0.2 3,537,827 - -0.2 3,537,868 - -0.3 3,537,868 - -1.3 3,537,868 - +0.6 3,537,868 - +0.5 3,537,934 + -0.4 3,537,943 - -0.2 3,537,943 - -0.5 3,537,964 - +0.7 3,537,964 - -0.3 3,537,987 - -0.5 3,537,987 - -0.7 3,537,987 - +0.4 3,537,990 + -0.4 3,538,000 + +1.5 3,538,000 + -0.3 3,538,034 + -0.6 3,538,036 + +0.0 3,538,036 + +1.0 3,538,042 - -0.5 3,538,044 - +0.5 3,538,044 - +1.2 3,538,080 + -1.3 3,538,080 + +0.8 3,538,111 + -0.9 3,538,119 - -0.8 3,538,123 + -0.1 3,538,140 + +0.7 3,538,140 + -2.2 3,538,142 + +0.1 3,538,142 + -0.9 3,538,150 + +0.4 3,538,155 - -1.2 3,538,163 + -0.1 3,538,172 + -0.9 3,538,172 + +0.4 3,538,172 - -0.9 3,538,185 + +0.4 3,538,185 + -0.8 3,538,193 - -1.8 3,538,193 - -0.7 3,538,193 - -0.3 3,538,195 + -1.3 3,538,204 - +0.7 3,538,204 - +0.0 3,538,219 - feoA b3408 0.15 -0.9 3,538,240 + feoA b3408 0.24 +0.2 3,538,240 + feoA b3408 0.24 -0.6 3,538,267 + feoA b3408 0.36 -0.6 3,538,273 + feoA b3408 0.39 -0.4 3,538,273 + feoA b3408 0.39 +0.1 3,538,275 - feoA b3408 0.39 -0.7 3,538,275 - feoA b3408 0.39 -0.6 3,538,277 + feoA b3408 0.40 -0.8 3,538,277 + feoA b3408 0.40 -1.2 3,538,279 + feoA b3408 0.41 -0.8 3,538,291 + feoA b3408 0.46 +0.4 3,538,297 + feoA b3408 0.49 -0.8 3,538,299 + feoA b3408 0.50 -0.2 3,538,299 - feoA b3408 0.50 -0.3 3,538,301 + feoA b3408 0.51 -1.9 3,538,301 + feoA b3408 0.51 -0.6 3,538,301 + feoA b3408 0.51 -2.2 3,538,306 + feoA b3408 0.53 -0.9 3,538,307 - feoA b3408 0.54 -0.7 3,538,309 + feoA b3408 0.54 +0.3 3,538,311 + feoA b3408 0.55 -1.1 3,538,311 + feoA b3408 0.55 -1.4 3,538,319 + feoA b3408 0.59 +0.0 3,538,319 - feoA b3408 0.59 -1.0 3,538,319 - feoA b3408 0.59 -0.8 3,538,319 - feoA b3408 0.59 -0.6 3,538,319 - feoA b3408 0.59 -0.3 3,538,319 - feoA b3408 0.59 +1.8 3,538,324 + feoA b3408 0.61 -1.3 3,538,332 - feoA b3408 0.64 -0.7 3,538,335 + feoA b3408 0.66 -0.1 3,538,335 + feoA b3408 0.66 -0.3 3,538,336 - feoA b3408 0.66 -1.3 3,538,336 - +0.0 3,538,336 - feoA b3408 0.66 -1.0 3,538,339 + feoA b3408 0.68 +0.4 3,538,342 + feoA b3408 0.69 -1.3 3,538,342 + feoA b3408 0.69 -0.9 3,538,342 - feoA b3408 0.69 -0.3 3,538,344 - feoA b3408 0.70 -0.6 3,538,348 + feoA b3408 0.71 -0.4 3,538,348 + feoA b3408 0.71 -0.5 3,538,352 - feoA b3408 0.73 -1.3 3,538,352 - feoA b3408 0.73 +1.2 3,538,353 + feoA b3408 0.74 -1.2 3,538,353 + feoA b3408 0.74 -0.4 3,538,359 + feoA b3408 0.76 -0.5 3,538,361 - feoA b3408 0.77 -1.4 3,538,362 - feoA b3408 0.78 -1.2 3,538,362 - feoA b3408 0.78 +0.2 3,538,362 - feoA b3408 0.78 -0.1 3,538,362 - feoA b3408 0.78 -0.8 3,538,384 + feoA b3408 0.87 -1.2 3,538,384 + feoA b3408 0.87 -2.6 3,538,392 - -0.4 3,538,392 - +0.5 3,538,417 - -2.4 3,538,417 - -0.0 3,538,455 - -0.4 3,538,494 + +0.6 3,538,494 + -0.6 3,538,494 + -1.2 3,538,501 + -0.5 3,538,501 + +0.0 3,538,507 + -1.3 3,538,511 + -2.8 3,538,512 + +1.0 3,538,512 + -1.2 3,538,515 - -0.2 3,538,516 + -0.2 3,538,516 + -1.5 3,538,517 - +0.4 3,538,517 - -0.9 3,538,518 + +0.2 3,538,524 - -0.2 3,538,526 - -1.3 3,538,532 + -0.8 3,538,532 + -0.2 3,538,534 - -0.7 3,538,539 - -1.3 3,538,540 - +1.0 3,538,540 - -0.3 3,538,540 - -0.4 3,538,540 - -0.2 3,538,546 + +0.2 3,538,563 + -1.8 3,538,563 + -1.4 3,538,563 + +0.4 3,538,568 - -0.3 3,538,571 - -0.6 3,538,586 + -0.8 3,538,586 + -0.6 3,538,586 + -0.7 3,538,588 + -1.7 3,538,589 - -1.1 3,538,593 - -1.3 3,538,596 - -0.1 3,538,597 - -0.3 3,538,597 - -0.7 3,538,607 - -1.2 3,538,615 - +0.2 3,538,633 - -0.2 3,538,633 - -0.2 3,538,635 + -0.6 3,538,650 - -0.4 3,538,665 - feoB b3409 0.10 -0.4 3,538,665 - feoB b3409 0.10 -1.2 3,538,669 + +2.0 3,538,669 + feoB b3409 0.10 -0.2 3,538,673 - feoB b3409 0.11 +0.7 3,538,712 + feoB b3409 0.12 +0.0 3,538,719 + feoB b3409 0.12 -0.3 3,538,738 - feoB b3409 0.13 -1.5 3,538,738 - feoB b3409 0.13 -1.5 3,538,744 + feoB b3409 0.14 -0.2 3,538,750 + feoB b3409 0.14 +1.0 3,538,750 + feoB b3409 0.14 -0.1 3,538,767 + feoB b3409 0.15 -0.6 3,538,775 - feoB b3409 0.15 -0.7 3,538,783 - feoB b3409 0.15 -1.9 3,538,793 - feoB b3409 0.16 -0.8 3,538,793 - feoB b3409 0.16 -0.1 3,538,807 - feoB b3409 0.16 -1.3 3,538,807 - feoB b3409 0.16 -1.0 3,538,846 + feoB b3409 0.18 -0.6 3,538,846 + feoB b3409 0.18 -2.2 3,538,846 + feoB b3409 0.18 -1.0 3,538,854 - feoB b3409 0.18 +1.2 3,538,854 - feoB b3409 0.18 -0.2 3,538,854 - feoB b3409 0.18 -1.0 3,538,923 + feoB b3409 0.21 -0.6 3,538,946 - feoB b3409 0.22 -0.6 3,538,955 + feoB b3409 0.23 -0.4 3,538,965 + feoB b3409 0.23 -0.7 3,539,031 + feoB b3409 0.26 -0.7 3,539,094 + feoB b3409 0.29 +0.9 3,539,149 + feoB b3409 0.31 -0.6 3,539,149 + feoB b3409 0.31 -0.3 3,539,157 - feoB b3409 0.31 -0.1 3,539,157 - feoB b3409 0.31 -1.1 3,539,176 - feoB b3409 0.32 -1.2 3,539,186 - feoB b3409 0.33 +1.4 3,539,186 - feoB b3409 0.33 -1.8 3,539,205 + feoB b3409 0.33 +0.1 3,539,214 + feoB b3409 0.34 -2.1 3,539,217 + feoB b3409 0.34 -0.3 3,539,217 + feoB b3409 0.34 +0.2 3,539,227 - feoB b3409 0.34 -2.1 3,539,258 - feoB b3409 0.36 -2.0 3,539,260 - feoB b3409 0.36 -0.6 3,539,260 - feoB b3409 0.36 +0.3 3,539,296 + feoB b3409 0.37 -0.5 3,539,343 + feoB b3409 0.39 -0.1 3,539,353 + feoB b3409 0.40 -1.2 3,539,394 + feoB b3409 0.42 -1.1
Or see this region's nucleotide sequence