Experiment: LB Anaerobic with Ramoplanin 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ydhH and slyB are separated by 273 nucleotides slyB and slyA are separated by 46 nucleotides slyA and ydhI are separated by 200 nucleotides ydhI and b1644 are separated by 2 nucleotides
b1640: ydhH - anhydro-N-acetylmuramic acid kinase (NCBI), at 1,716,517 to 1,717,626
ydhH
b1641: slyB - outer membrane lipoprotein (NCBI), at 1,717,900 to 1,718,367
slyB
b1642: slyA - transcriptional regulator for cryptic hemolysin (VIMSS), at 1,718,414 to 1,718,848
slyA
b1643: ydhI - predicted inner membrane protein (NCBI), at 1,719,049 to 1,719,285
ydhI
b1644: b1644 - putative membrane protein (VIMSS), at 1,719,288 to 1,720,145
b1644
Position (kb)
1718
1719 Strain fitness (log2 ratio)
-2
-1
0
1 at 1717.569 kb on + strand at 1717.569 kb on + strand at 1717.612 kb on - strand at 1717.661 kb on + strand at 1717.661 kb on + strand at 1717.696 kb on - strand at 1717.837 kb on + strand at 1717.934 kb on + strand at 1718.034 kb on - strand, within slyB at 1718.718 kb on - strand, within slyA at 1718.718 kb on - strand, within slyA at 1718.809 kb on - strand at 1719.062 kb on - strand at 1719.065 kb on + strand at 1719.078 kb on + strand, within ydhI at 1719.103 kb on - strand, within ydhI at 1719.163 kb on + strand, within ydhI at 1719.171 kb on - strand, within ydhI at 1719.171 kb on - strand, within ydhI at 1719.190 kb on + strand, within ydhI at 1719.211 kb on + strand, within ydhI at 1719.221 kb on - strand, within ydhI at 1719.559 kb on - strand, within b1644 at 1719.779 kb on + strand, within b1644 at 1719.779 kb on + strand, within b1644 at 1719.786 kb on - strand, within b1644 at 1719.786 kb on - strand, within b1644 at 1719.832 kb on - strand, within b1644 at 1719.832 kb on - strand, within b1644
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Ramoplanin 0.00003125 mM remove 1,717,569 + +0.5 1,717,569 + +0.5 1,717,612 - -0.6 1,717,661 + -0.1 1,717,661 + +0.2 1,717,696 - -0.7 1,717,837 + -0.1 1,717,934 + +0.4 1,718,034 - slyB b1641 0.29 +0.9 1,718,718 - slyA b1642 0.70 -0.3 1,718,718 - slyA b1642 0.70 -1.5 1,718,809 - -0.2 1,719,062 - -0.2 1,719,065 + -0.1 1,719,078 + ydhI b1643 0.12 +0.3 1,719,103 - ydhI b1643 0.23 -0.8 1,719,163 + ydhI b1643 0.48 -0.4 1,719,171 - ydhI b1643 0.51 -0.5 1,719,171 - ydhI b1643 0.51 -0.2 1,719,190 + ydhI b1643 0.59 +0.1 1,719,211 + ydhI b1643 0.68 -0.3 1,719,221 - ydhI b1643 0.73 -2.1 1,719,559 - b1644 b1644 0.32 -1.4 1,719,779 + b1644 b1644 0.57 -0.3 1,719,779 + b1644 b1644 0.57 -0.6 1,719,786 - b1644 b1644 0.58 +1.0 1,719,786 - b1644 b1644 0.58 -0.7 1,719,832 - b1644 b1644 0.63 -1.3 1,719,832 - b1644 b1644 0.63 -0.1
Or see this region's nucleotide sequence