Strain Fitness in Pseudomonas putida KT2440 around PP_1020

Experiment: D-Glucose (C) 1.25 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPP_1019 and PP_1020 are separated by 117 nucleotidesPP_1020 and PP_1021 overlap by 8 nucleotides PP_1019: PP_1019 - carbohydrate-selective porin, at 1,162,233 to 1,163,576 _1019 PP_1020: PP_1020 - putative glucose-6-phosphate 1-epimerase, at 1,163,694 to 1,164,548 _1020 PP_1021: PP_1021 - DNA-binding transcriptional regulator, at 1,164,541 to 1,165,404 _1021 Position (kb) 1163 1164 1165Strain fitness (log2 ratio) -3 -2 -1 0 1at 1162.854 kb on + strand, within PP_1019at 1162.854 kb on + strand, within PP_1019at 1162.855 kb on - strand, within PP_1019at 1162.855 kb on - strand, within PP_1019at 1162.855 kb on - strand, within PP_1019at 1162.855 kb on - strand, within PP_1019at 1162.880 kb on - strand, within PP_1019at 1162.880 kb on - strand, within PP_1019at 1162.952 kb on + strand, within PP_1019at 1162.952 kb on + strand, within PP_1019at 1163.034 kb on + strand, within PP_1019at 1163.034 kb on + strand, within PP_1019at 1163.034 kb on + strand, within PP_1019at 1163.034 kb on + strand, within PP_1019at 1163.035 kb on - strand, within PP_1019at 1163.035 kb on - strand, within PP_1019at 1163.035 kb on - strand, within PP_1019at 1163.037 kb on + strandat 1163.037 kb on + strand, within PP_1019at 1163.037 kb on + strand, within PP_1019at 1163.037 kb on + strand, within PP_1019at 1163.038 kb on - strand, within PP_1019at 1163.064 kb on + strand, within PP_1019at 1163.065 kb on - strand, within PP_1019at 1163.250 kb on + strand, within PP_1019at 1163.251 kb on - strand, within PP_1019at 1163.271 kb on + strand, within PP_1019at 1163.271 kb on + strand, within PP_1019at 1163.327 kb on + strand, within PP_1019at 1163.426 kb on + strand, within PP_1019at 1163.427 kb on - strand, within PP_1019at 1163.433 kb on + strand, within PP_1019at 1163.434 kb on - strand, within PP_1019at 1163.448 kb on + strandat 1163.448 kb on + strandat 1163.448 kb on + strandat 1163.448 kb on + strandat 1163.448 kb on + strandat 1163.448 kb on + strandat 1163.449 kb on - strandat 1163.499 kb on + strandat 1163.500 kb on - strandat 1163.500 kb on - strandat 1163.941 kb on - strand, within PP_1020at 1164.307 kb on - strand, within PP_1020at 1164.307 kb on - strand, within PP_1020at 1164.655 kb on + strand, within PP_1021at 1164.656 kb on - strand, within PP_1021at 1164.656 kb on - strand, within PP_1021at 1164.701 kb on + strand, within PP_1021at 1164.701 kb on + strand, within PP_1021at 1164.702 kb on - strand, within PP_1021at 1164.702 kb on - strand, within PP_1021at 1164.702 kb on - strand, within PP_1021at 1165.148 kb on - strand, within PP_1021at 1165.163 kb on + strand, within PP_1021at 1165.163 kb on + strand, within PP_1021at 1165.479 kb on - strandat 1165.503 kb on + strandat 1165.529 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction D-Glucose (C) 1.25 mM
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1,162,854 + PP_1019 0.46 -1.6
1,162,854 + PP_1019 0.46 -0.9
1,162,855 - PP_1019 0.46 -0.1
1,162,855 - PP_1019 0.46 +0.5
1,162,855 - PP_1019 0.46 +0.4
1,162,855 - PP_1019 0.46 -0.9
1,162,880 - PP_1019 0.48 -0.8
1,162,880 - PP_1019 0.48 +0.2
1,162,952 + PP_1019 0.53 -1.8
1,162,952 + PP_1019 0.53 +0.4
1,163,034 + PP_1019 0.60 -0.1
1,163,034 + PP_1019 0.60 -1.0
1,163,034 + PP_1019 0.60 -0.5
1,163,034 + PP_1019 0.60 -0.6
1,163,035 - PP_1019 0.60 -0.4
1,163,035 - PP_1019 0.60 -0.4
1,163,035 - PP_1019 0.60 -1.6
1,163,037 + -0.7
1,163,037 + PP_1019 0.60 -0.0
1,163,037 + PP_1019 0.60 -0.8
1,163,037 + PP_1019 0.60 -2.6
1,163,038 - PP_1019 0.60 +0.0
1,163,064 + PP_1019 0.62 -0.8
1,163,065 - PP_1019 0.62 -2.8
1,163,250 + PP_1019 0.76 -0.9
1,163,251 - PP_1019 0.76 -0.9
1,163,271 + PP_1019 0.77 +0.0
1,163,271 + PP_1019 0.77 -0.3
1,163,327 + PP_1019 0.81 +0.8
1,163,426 + PP_1019 0.89 +0.3
1,163,427 - PP_1019 0.89 +0.1
1,163,433 + PP_1019 0.89 -1.4
1,163,434 - PP_1019 0.89 +0.2
1,163,448 + -0.5
1,163,448 + +0.6
1,163,448 + -0.2
1,163,448 + -1.2
1,163,448 + +1.6
1,163,448 + -0.1
1,163,449 - -0.1
1,163,499 + -0.5
1,163,500 - +0.4
1,163,500 - -1.2
1,163,941 - PP_1020 0.29 -0.1
1,164,307 - PP_1020 0.72 -0.9
1,164,307 - PP_1020 0.72 +0.4
1,164,655 + PP_1021 0.13 -0.9
1,164,656 - PP_1021 0.13 +0.9
1,164,656 - PP_1021 0.13 -0.8
1,164,701 + PP_1021 0.19 +0.3
1,164,701 + PP_1021 0.19 +0.5
1,164,702 - PP_1021 0.19 -0.4
1,164,702 - PP_1021 0.19 +0.8
1,164,702 - PP_1021 0.19 +0.6
1,165,148 - PP_1021 0.70 -1.1
1,165,163 + PP_1021 0.72 -1.1
1,165,163 + PP_1021 0.72 +1.2
1,165,479 - -1.2
1,165,503 + +0.6
1,165,529 + -0.5

Or see this region's nucleotide sequence