Strain Fitness in Escherichia coli BW25113 around b4175

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthflK and hflC are separated by 2 nucleotideshflC and yjeT are separated by 81 nucleotidesyjeT and purA are separated by 103 nucleotides b4174: hflK - modulator for HflB protease specific for phage lambda cII repressor (NCBI), at 4,400,061 to 4,401,320 hflK b4175: hflC - modulator for HflB protease specific for phage lambda cII repressor (NCBI), at 4,401,323 to 4,402,327 hflC b4176: yjeT - conserved inner membrane protein (NCBI), at 4,402,409 to 4,402,606 yjeT b4177: purA - adenylosuccinate synthetase (NCBI), at 4,402,710 to 4,404,008 purA Position (kb) 4401 4402 4403Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4400.356 kb on + strand, within hflKat 4400.385 kb on - strand, within hflKat 4400.385 kb on - strand, within hflKat 4400.411 kb on + strand, within hflKat 4400.416 kb on + strand, within hflKat 4400.416 kb on + strand, within hflKat 4400.429 kb on - strand, within hflKat 4400.476 kb on - strand, within hflKat 4400.502 kb on - strand, within hflKat 4400.534 kb on - strand, within hflKat 4400.546 kb on - strand, within hflKat 4400.555 kb on - strand, within hflKat 4400.582 kb on + strand, within hflKat 4400.586 kb on + strand, within hflKat 4400.586 kb on + strand, within hflKat 4400.620 kb on - strand, within hflKat 4400.643 kb on + strand, within hflKat 4400.654 kb on + strand, within hflKat 4400.704 kb on - strand, within hflKat 4400.704 kb on - strand, within hflKat 4400.709 kb on - strand, within hflKat 4400.716 kb on + strand, within hflKat 4400.716 kb on + strand, within hflKat 4400.747 kb on + strand, within hflKat 4400.749 kb on + strand, within hflKat 4400.767 kb on - strand, within hflKat 4400.779 kb on - strand, within hflKat 4400.779 kb on - strand, within hflKat 4400.860 kb on + strand, within hflKat 4400.875 kb on - strand, within hflKat 4400.908 kb on - strand, within hflKat 4400.932 kb on + strand, within hflKat 4400.933 kb on + strand, within hflKat 4400.942 kb on + strand, within hflKat 4400.942 kb on + strand, within hflKat 4400.942 kb on - strand, within hflKat 4400.951 kb on - strand, within hflKat 4400.951 kb on - strand, within hflKat 4400.952 kb on + strand, within hflKat 4400.952 kb on + strand, within hflKat 4400.952 kb on + strand, within hflKat 4400.966 kb on + strand, within hflKat 4400.983 kb on + strand, within hflKat 4400.991 kb on - strand, within hflKat 4401.008 kb on + strand, within hflKat 4401.010 kb on - strand, within hflKat 4401.073 kb on - strand, within hflKat 4401.091 kb on - strand, within hflKat 4401.091 kb on - strand, within hflKat 4401.218 kb on + strandat 4401.238 kb on - strandat 4401.238 kb on - strandat 4401.252 kb on - strandat 4401.259 kb on + strandat 4401.310 kb on + strandat 4401.362 kb on + strandat 4401.368 kb on + strandat 4401.368 kb on + strandat 4401.408 kb on + strandat 4401.408 kb on + strandat 4401.408 kb on + strandat 4401.408 kb on + strandat 4401.426 kb on + strand, within hflCat 4401.436 kb on + strand, within hflCat 4401.467 kb on - strand, within hflCat 4401.526 kb on + strand, within hflCat 4401.605 kb on - strand, within hflCat 4401.628 kb on + strand, within hflCat 4401.639 kb on - strand, within hflCat 4401.643 kb on - strand, within hflCat 4401.756 kb on - strand, within hflCat 4401.764 kb on - strand, within hflCat 4401.764 kb on - strand, within hflCat 4401.792 kb on - strand, within hflCat 4401.792 kb on - strand, within hflCat 4401.910 kb on + strand, within hflCat 4401.912 kb on + strand, within hflCat 4401.912 kb on + strand, within hflCat 4401.912 kb on + strand, within hflCat 4401.912 kb on + strand, within hflCat 4401.920 kb on - strand, within hflCat 4401.920 kb on - strand, within hflCat 4401.920 kb on - strand, within hflCat 4401.920 kb on - strand, within hflCat 4401.927 kb on + strand, within hflCat 4401.958 kb on + strand, within hflCat 4401.986 kb on - strand, within hflCat 4401.986 kb on - strand, within hflCat 4401.997 kb on - strand, within hflCat 4401.997 kb on - strand, within hflCat 4402.038 kb on + strand, within hflCat 4402.038 kb on + strand, within hflCat 4402.126 kb on - strand, within hflCat 4402.126 kb on - strand, within hflCat 4402.138 kb on - strand, within hflCat 4402.166 kb on - strand, within hflCat 4402.191 kb on - strand, within hflCat 4402.220 kb on - strand, within hflCat 4402.220 kb on - strand, within hflCat 4402.253 kb on - strandat 4402.253 kb on - strandat 4402.258 kb on + strandat 4402.263 kb on - strandat 4402.263 kb on - strandat 4402.263 kb on - strandat 4402.263 kb on - strandat 4402.326 kb on + strandat 4402.336 kb on + strandat 4402.336 kb on + strandat 4402.341 kb on + strandat 4402.373 kb on + strandat 4402.441 kb on + strand, within yjeTat 4402.449 kb on - strand, within yjeTat 4402.484 kb on - strand, within yjeTat 4402.497 kb on - strand, within yjeTat 4402.930 kb on - strand, within purAat 4402.990 kb on - strand, within purAat 4403.242 kb on - strand, within purA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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4,400,356 + hflK b4174 0.23 +1.1
4,400,385 - hflK b4174 0.26 +0.5
4,400,385 - hflK b4174 0.26 +0.7
4,400,411 + hflK b4174 0.28 +1.0
4,400,416 + hflK b4174 0.28 +0.5
4,400,416 + hflK b4174 0.28 +0.3
4,400,429 - hflK b4174 0.29 +1.4
4,400,476 - hflK b4174 0.33 +0.9
4,400,502 - hflK b4174 0.35 +0.1
4,400,534 - hflK b4174 0.38 +0.1
4,400,546 - hflK b4174 0.38 +0.0
4,400,555 - hflK b4174 0.39 +0.5
4,400,582 + hflK b4174 0.41 +0.8
4,400,586 + hflK b4174 0.42 +1.9
4,400,586 + hflK b4174 0.42 +0.7
4,400,620 - hflK b4174 0.44 +1.8
4,400,643 + hflK b4174 0.46 +0.8
4,400,654 + hflK b4174 0.47 +0.1
4,400,704 - hflK b4174 0.51 +1.2
4,400,704 - hflK b4174 0.51 -0.1
4,400,709 - hflK b4174 0.51 +2.5
4,400,716 + hflK b4174 0.52 +1.9
4,400,716 + hflK b4174 0.52 +0.7
4,400,747 + hflK b4174 0.54 +0.6
4,400,749 + hflK b4174 0.55 +1.3
4,400,767 - hflK b4174 0.56 +0.9
4,400,779 - hflK b4174 0.57 +2.5
4,400,779 - hflK b4174 0.57 +1.5
4,400,860 + hflK b4174 0.63 +1.3
4,400,875 - hflK b4174 0.65 +1.5
4,400,908 - hflK b4174 0.67 -0.2
4,400,932 + hflK b4174 0.69 +1.9
4,400,933 + hflK b4174 0.69 +3.7
4,400,942 + hflK b4174 0.70 +0.8
4,400,942 + hflK b4174 0.70 +0.1
4,400,942 - hflK b4174 0.70 +1.5
4,400,951 - hflK b4174 0.71 -0.5
4,400,951 - hflK b4174 0.71 +0.5
4,400,952 + hflK b4174 0.71 +2.4
4,400,952 + hflK b4174 0.71 +0.1
4,400,952 + hflK b4174 0.71 +1.3
4,400,966 + hflK b4174 0.72 +0.7
4,400,983 + hflK b4174 0.73 +1.7
4,400,991 - hflK b4174 0.74 +0.6
4,401,008 + hflK b4174 0.75 +0.4
4,401,010 - hflK b4174 0.75 +1.8
4,401,073 - hflK b4174 0.80 +1.6
4,401,091 - hflK b4174 0.82 +0.5
4,401,091 - hflK b4174 0.82 -1.2
4,401,218 + +1.0
4,401,238 - +1.1
4,401,238 - -0.1
4,401,252 - +1.7
4,401,259 + -1.5
4,401,310 + -0.3
4,401,362 + +1.7
4,401,368 + +0.7
4,401,368 + +2.8
4,401,408 + +1.6
4,401,408 + +1.3
4,401,408 + +1.1
4,401,408 + +1.6
4,401,426 + hflC b4175 0.10 +0.0
4,401,436 + hflC b4175 0.11 +2.2
4,401,467 - hflC b4175 0.14 +0.7
4,401,526 + hflC b4175 0.20 +0.9
4,401,605 - hflC b4175 0.28 +2.0
4,401,628 + hflC b4175 0.30 -2.6
4,401,639 - hflC b4175 0.31 +0.9
4,401,643 - hflC b4175 0.32 +0.5
4,401,756 - hflC b4175 0.43 +1.8
4,401,764 - hflC b4175 0.44 +1.4
4,401,764 - hflC b4175 0.44 +2.3
4,401,792 - hflC b4175 0.47 +2.5
4,401,792 - hflC b4175 0.47 +1.4
4,401,910 + hflC b4175 0.58 +1.8
4,401,912 + hflC b4175 0.59 +1.1
4,401,912 + hflC b4175 0.59 +1.0
4,401,912 + hflC b4175 0.59 +1.0
4,401,912 + hflC b4175 0.59 +1.2
4,401,920 - hflC b4175 0.59 +0.5
4,401,920 - hflC b4175 0.59 +0.9
4,401,920 - hflC b4175 0.59 +2.7
4,401,920 - hflC b4175 0.59 +0.5
4,401,927 + hflC b4175 0.60 +0.5
4,401,958 + hflC b4175 0.63 +0.9
4,401,986 - hflC b4175 0.66 +1.3
4,401,986 - hflC b4175 0.66 +1.4
4,401,997 - hflC b4175 0.67 +1.0
4,401,997 - hflC b4175 0.67 +0.7
4,402,038 + hflC b4175 0.71 -0.5
4,402,038 + hflC b4175 0.71 +1.0
4,402,126 - hflC b4175 0.80 +0.4
4,402,126 - hflC b4175 0.80 +1.4
4,402,138 - hflC b4175 0.81 -0.1
4,402,166 - hflC b4175 0.84 -0.0
4,402,191 - hflC b4175 0.86 +0.7
4,402,220 - hflC b4175 0.89 +0.5
4,402,220 - hflC b4175 0.89 +0.4
4,402,253 - +0.3
4,402,253 - +1.2
4,402,258 + +0.9
4,402,263 - -0.3
4,402,263 - +1.0
4,402,263 - +1.8
4,402,263 - +1.6
4,402,326 + +1.3
4,402,336 + +0.4
4,402,336 + -0.2
4,402,341 + +0.8
4,402,373 + +1.0
4,402,441 + yjeT b4176 0.16 -0.3
4,402,449 - yjeT b4176 0.20 -0.6
4,402,484 - yjeT b4176 0.38 -0.5
4,402,497 - yjeT b4176 0.44 +0.1
4,402,930 - purA b4177 0.17 +1.1
4,402,990 - purA b4177 0.22 +2.1
4,403,242 - purA b4177 0.41 +1.4

Or see this region's nucleotide sequence