Strain Fitness in Escherichia coli BW25113 around b4171

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmutL and miaA overlap by 8 nucleotidesmiaA and hfq are separated by 85 nucleotideshfq and hflX are separated by 75 nucleotides b4170: mutL - DNA mismatch repair protein (NCBI), at 4,395,435 to 4,397,282 mutL b4171: miaA - tRNA delta(2)-isopentenylpyrophosphate transferase (NCBI), at 4,397,275 to 4,398,225 miaA b4172: hfq - RNA-binding protein Hfq (NCBI), at 4,398,311 to 4,398,619 hfq b4173: hflX - predicted GTPase (NCBI), at 4,398,695 to 4,399,975 hflX Position (kb) 4397 4398 4399Strain fitness (log2 ratio) -2 -1 0 1 2 3 4at 4396.326 kb on - strand, within mutLat 4396.329 kb on - strand, within mutLat 4396.407 kb on + strand, within mutLat 4396.415 kb on + strand, within mutLat 4396.415 kb on - strand, within mutLat 4396.432 kb on - strand, within mutLat 4396.435 kb on - strand, within mutLat 4396.435 kb on - strand, within mutLat 4396.466 kb on - strand, within mutLat 4396.546 kb on + strand, within mutLat 4396.551 kb on + strand, within mutLat 4396.556 kb on + strand, within mutLat 4396.556 kb on + strand, within mutLat 4396.556 kb on + strand, within mutLat 4396.778 kb on + strand, within mutLat 4396.991 kb on + strand, within mutLat 4396.991 kb on + strand, within mutLat 4396.991 kb on + strand, within mutLat 4396.999 kb on - strand, within mutLat 4396.999 kb on - strand, within mutLat 4396.999 kb on - strand, within mutLat 4396.999 kb on - strand, within mutLat 4397.012 kb on + strand, within mutLat 4397.162 kb on + strandat 4397.162 kb on + strandat 4397.162 kb on + strandat 4397.162 kb on + strandat 4397.170 kb on - strandat 4397.195 kb on - strandat 4397.195 kb on - strandat 4397.244 kb on + strandat 4397.244 kb on + strandat 4397.420 kb on + strand, within miaAat 4397.449 kb on + strand, within miaAat 4397.449 kb on + strand, within miaAat 4397.455 kb on + strand, within miaAat 4397.458 kb on - strand, within miaAat 4397.505 kb on + strand, within miaAat 4397.549 kb on - strand, within miaAat 4397.584 kb on + strand, within miaAat 4397.589 kb on - strand, within miaAat 4397.704 kb on - strand, within miaAat 4397.763 kb on - strand, within miaAat 4397.845 kb on - strand, within miaAat 4397.853 kb on + strand, within miaAat 4397.856 kb on + strand, within miaAat 4397.866 kb on + strand, within miaAat 4397.922 kb on + strand, within miaAat 4397.935 kb on + strand, within miaAat 4397.935 kb on + strand, within miaAat 4398.137 kb on + strandat 4398.137 kb on + strandat 4398.156 kb on + strandat 4398.233 kb on + strandat 4398.233 kb on + strandat 4398.433 kb on + strand, within hfqat 4398.562 kb on + strand, within hfqat 4398.565 kb on + strand, within hfqat 4398.593 kb on + strandat 4398.668 kb on - strandat 4398.675 kb on - strandat 4398.677 kb on + strandat 4398.677 kb on + strandat 4398.703 kb on + strandat 4398.714 kb on + strandat 4398.724 kb on - strandat 4398.728 kb on + strandat 4398.728 kb on + strandat 4398.793 kb on + strandat 4398.815 kb on - strandat 4398.815 kb on - strandat 4398.837 kb on + strand, within hflXat 4398.926 kb on - strand, within hflXat 4398.961 kb on + strand, within hflXat 4398.961 kb on + strand, within hflXat 4398.965 kb on + strand, within hflXat 4398.965 kb on + strand, within hflXat 4398.968 kb on - strand, within hflXat 4398.983 kb on - strand, within hflXat 4398.983 kb on - strand, within hflXat 4398.992 kb on - strand, within hflXat 4399.118 kb on + strand, within hflXat 4399.140 kb on + strand, within hflXat 4399.154 kb on + strand, within hflXat 4399.217 kb on - strand, within hflX

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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4,396,326 - mutL b4170 0.48 -0.0
4,396,329 - mutL b4170 0.48 -0.6
4,396,407 + mutL b4170 0.53 -0.0
4,396,415 + mutL b4170 0.53 +1.8
4,396,415 - mutL b4170 0.53 +0.1
4,396,432 - mutL b4170 0.54 +0.1
4,396,435 - mutL b4170 0.54 +0.0
4,396,435 - mutL b4170 0.54 +0.8
4,396,466 - mutL b4170 0.56 -0.4
4,396,546 + mutL b4170 0.60 -1.3
4,396,551 + mutL b4170 0.60 -0.2
4,396,556 + mutL b4170 0.61 +0.6
4,396,556 + mutL b4170 0.61 +0.3
4,396,556 + mutL b4170 0.61 +1.2
4,396,778 + mutL b4170 0.73 -2.6
4,396,991 + mutL b4170 0.84 +0.1
4,396,991 + mutL b4170 0.84 +0.6
4,396,991 + mutL b4170 0.84 +0.7
4,396,999 - mutL b4170 0.85 -1.0
4,396,999 - mutL b4170 0.85 +0.9
4,396,999 - mutL b4170 0.85 -0.6
4,396,999 - mutL b4170 0.85 +0.8
4,397,012 + mutL b4170 0.85 -0.1
4,397,162 + +1.1
4,397,162 + +0.9
4,397,162 + +1.3
4,397,162 + +0.2
4,397,170 - -0.1
4,397,195 - -0.1
4,397,195 - +1.1
4,397,244 + -1.3
4,397,244 + +0.7
4,397,420 + miaA b4171 0.15 -0.9
4,397,449 + miaA b4171 0.18 -0.5
4,397,449 + miaA b4171 0.18 +1.1
4,397,455 + miaA b4171 0.19 -1.2
4,397,458 - miaA b4171 0.19 -1.5
4,397,505 + miaA b4171 0.24 +2.6
4,397,549 - miaA b4171 0.29 +2.1
4,397,584 + miaA b4171 0.32 +4.1
4,397,589 - miaA b4171 0.33 +0.5
4,397,704 - miaA b4171 0.45 +1.1
4,397,763 - miaA b4171 0.51 +2.1
4,397,845 - miaA b4171 0.60 +0.5
4,397,853 + miaA b4171 0.61 +0.5
4,397,856 + miaA b4171 0.61 -0.2
4,397,866 + miaA b4171 0.62 -1.0
4,397,922 + miaA b4171 0.68 +2.6
4,397,935 + miaA b4171 0.69 +0.3
4,397,935 + miaA b4171 0.69 +4.1
4,398,137 + +1.1
4,398,137 + +3.0
4,398,156 + +2.1
4,398,233 + +1.6
4,398,233 + +2.8
4,398,433 + hfq b4172 0.39 +2.1
4,398,562 + hfq b4172 0.81 +0.5
4,398,565 + hfq b4172 0.82 +0.1
4,398,593 + -0.5
4,398,668 - +1.2
4,398,675 - -2.3
4,398,677 + -1.0
4,398,677 + -0.7
4,398,703 + +0.3
4,398,714 + +0.9
4,398,724 - +0.7
4,398,728 + -1.0
4,398,728 + -1.6
4,398,793 + +0.3
4,398,815 - -1.6
4,398,815 - -0.6
4,398,837 + hflX b4173 0.11 -0.7
4,398,926 - hflX b4173 0.18 +1.5
4,398,961 + hflX b4173 0.21 -1.6
4,398,961 + hflX b4173 0.21 +0.3
4,398,965 + hflX b4173 0.21 -0.5
4,398,965 + hflX b4173 0.21 +0.5
4,398,968 - hflX b4173 0.21 +1.7
4,398,983 - hflX b4173 0.22 +1.1
4,398,983 - hflX b4173 0.22 +1.1
4,398,992 - hflX b4173 0.23 +1.6
4,399,118 + hflX b4173 0.33 -1.5
4,399,140 + hflX b4173 0.35 -1.3
4,399,154 + hflX b4173 0.36 -1.0
4,399,217 - hflX b4173 0.41 -0.0

Or see this region's nucleotide sequence