Strain Fitness in Escherichia coli BW25113 around b4025
Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM |
---|---|---|---|---|---|
remove | |||||
4,230,783 | + | lysC | b4024 | 0.65 | -0.6 |
4,230,788 | + | lysC | b4024 | 0.65 | +0.3 |
4,230,843 | - | lysC | b4024 | 0.69 | +0.1 |
4,231,007 | - | lysC | b4024 | 0.81 | -0.9 |
4,231,015 | + | lysC | b4024 | 0.82 | -0.2 |
4,231,126 | - | +0.4 | |||
4,231,140 | - | +0.4 | |||
4,231,140 | - | +0.1 | |||
4,231,194 | + | +0.7 | |||
4,231,194 | + | +0.6 | |||
4,231,200 | - | +2.2 | |||
4,231,224 | - | +1.0 | |||
4,231,224 | - | +1.0 | |||
4,231,254 | + | +0.7 | |||
4,231,254 | + | +0.6 | |||
4,231,293 | + | +1.7 | |||
4,231,331 | + | +1.4 | |||
4,231,446 | - | +0.4 | |||
4,231,485 | - | -0.8 | |||
4,231,499 | + | -0.7 | |||
4,231,524 | - | +0.7 | |||
4,231,524 | - | -1.1 | |||
4,231,570 | + | -0.3 | |||
4,231,570 | + | -0.2 | |||
4,231,590 | + | -0.5 | |||
4,231,699 | - | -0.6 | |||
4,231,859 | - | -1.9 | |||
4,231,936 | - | -2.1 | |||
4,231,936 | - | -1.0 | |||
4,231,968 | - | pgi | b4025 | 0.11 | -2.0 |
4,232,022 | - | pgi | b4025 | 0.15 | -0.4 |
4,232,033 | + | pgi | b4025 | 0.15 | -1.1 |
4,232,074 | - | pgi | b4025 | 0.18 | -3.0 |
4,232,074 | - | pgi | b4025 | 0.18 | -3.0 |
4,232,074 | - | pgi | b4025 | 0.18 | -2.2 |
4,232,137 | + | pgi | b4025 | 0.22 | -1.8 |
4,232,145 | - | pgi | b4025 | 0.22 | -1.0 |
4,232,200 | + | pgi | b4025 | 0.25 | -1.5 |
4,232,200 | + | pgi | b4025 | 0.25 | -1.6 |
4,232,208 | - | pgi | b4025 | 0.26 | +0.2 |
4,232,258 | - | pgi | b4025 | 0.29 | -2.6 |
4,232,258 | - | pgi | b4025 | 0.29 | -2.4 |
4,232,258 | - | pgi | b4025 | 0.29 | +0.2 |
4,232,283 | - | pgi | b4025 | 0.30 | -0.8 |
4,232,321 | + | pgi | b4025 | 0.33 | -1.9 |
4,232,321 | + | pgi | b4025 | 0.33 | -2.2 |
4,232,333 | + | pgi | b4025 | 0.33 | +0.2 |
4,232,394 | - | pgi | b4025 | 0.37 | +2.2 |
4,232,394 | - | pgi | b4025 | 0.37 | -1.5 |
4,232,398 | + | pgi | b4025 | 0.37 | -1.0 |
4,232,405 | - | pgi | b4025 | 0.38 | -0.6 |
4,232,405 | - | pgi | b4025 | 0.38 | -0.4 |
4,232,426 | - | pgi | b4025 | 0.39 | -1.7 |
4,232,426 | - | pgi | b4025 | 0.39 | -1.1 |
4,232,472 | + | pgi | b4025 | 0.42 | -2.7 |
4,232,506 | + | pgi | b4025 | 0.44 | -1.9 |
4,232,509 | + | pgi | b4025 | 0.44 | -0.4 |
4,232,509 | + | pgi | b4025 | 0.44 | +1.2 |
4,232,560 | - | pgi | b4025 | 0.47 | -1.3 |
4,232,560 | - | pgi | b4025 | 0.47 | +0.2 |
4,232,632 | - | pgi | b4025 | 0.52 | -2.1 |
4,232,637 | - | pgi | b4025 | 0.52 | -0.1 |
4,232,800 | + | pgi | b4025 | 0.62 | -1.1 |
4,232,807 | - | pgi | b4025 | 0.62 | -2.4 |
4,232,835 | + | pgi | b4025 | 0.64 | -1.0 |
4,232,895 | - | pgi | b4025 | 0.68 | -3.4 |
4,232,895 | - | pgi | b4025 | 0.68 | -1.4 |
4,232,895 | - | pgi | b4025 | 0.68 | -0.6 |
4,232,944 | + | pgi | b4025 | 0.70 | -1.0 |
4,233,014 | - | pgi | b4025 | 0.75 | -1.6 |
4,233,014 | - | pgi | b4025 | 0.75 | -2.3 |
4,233,027 | - | pgi | b4025 | 0.76 | -1.5 |
4,233,121 | + | pgi | b4025 | 0.81 | +0.6 |
4,233,121 | + | pgi | b4025 | 0.81 | -1.1 |
4,233,155 | - | pgi | b4025 | 0.83 | -1.1 |
4,233,184 | + | pgi | b4025 | 0.85 | -1.6 |
4,233,200 | - | pgi | b4025 | 0.86 | -1.5 |
4,233,200 | - | pgi | b4025 | 0.86 | -1.1 |
4,233,217 | + | pgi | b4025 | 0.87 | -0.4 |
4,233,217 | + | pgi | b4025 | 0.87 | -1.3 |
4,233,268 | + | -1.7 | |||
4,233,271 | - | -2.5 | |||
4,233,277 | + | -3.1 | |||
4,233,314 | - | -1.0 | |||
4,233,314 | - | -0.4 | |||
4,233,403 | - | -0.1 | |||
4,233,406 | + | -0.1 | |||
4,233,408 | - | -0.8 | |||
4,233,417 | - | -0.6 | |||
4,233,426 | + | +1.5 | |||
4,233,426 | + | +0.2 | |||
4,233,426 | + | -0.4 | |||
4,233,460 | - | -1.1 | |||
4,233,461 | + | -3.0 | |||
4,233,461 | + | -2.6 | |||
4,233,479 | + | -0.3 | |||
4,233,480 | - | -0.7 | |||
4,233,487 | - | -0.1 | |||
4,233,490 | + | +0.7 | |||
4,233,490 | + | +0.9 | |||
4,233,504 | + | -1.0 | |||
4,233,512 | + | +0.9 | |||
4,233,512 | - | -0.4 | |||
4,233,512 | - | -0.1 | |||
4,233,512 | - | -0.1 | |||
4,233,514 | + | -1.8 | |||
4,233,514 | + | +1.1 | |||
4,233,514 | + | +0.2 | |||
4,233,516 | + | -0.2 | |||
4,233,522 | - | +0.7 | |||
4,233,522 | - | -0.6 | |||
4,233,522 | - | -0.7 | |||
4,233,522 | - | -0.7 | |||
4,233,525 | - | -0.1 | |||
4,233,527 | + | +1.2 | |||
4,233,532 | + | +0.3 | |||
4,233,535 | + | +0.1 | |||
4,233,535 | - | +0.1 | |||
4,233,537 | - | -0.5 | |||
4,233,543 | - | +0.4 | |||
4,233,544 | - | -0.3 | |||
4,233,545 | - | +1.8 | |||
4,233,558 | + | +0.6 | |||
4,233,558 | + | -0.8 | |||
4,233,560 | + | +0.0 | |||
4,233,568 | - | +0.7 | |||
4,233,568 | - | -0.6 | |||
4,233,583 | + | -0.3 | |||
4,233,583 | + | -1.1 | |||
4,233,583 | + | +0.9 | |||
4,233,584 | + | +1.1 | |||
4,233,604 | - | -1.2 | |||
4,233,625 | + | +1.2 | |||
4,233,625 | + | -1.0 | |||
4,233,627 | + | +1.2 | |||
4,233,627 | + | +0.6 | |||
4,233,648 | - | +1.2 | |||
4,233,853 | + | +1.2 | |||
4,233,853 | + | +1.2 | |||
4,233,853 | + | +0.6 | |||
4,233,859 | - | +0.9 | |||
4,233,861 | + | +1.2 | |||
4,233,861 | + | +1.2 | |||
4,233,861 | + | +1.2 | |||
4,233,861 | + | +0.2 | |||
4,233,861 | - | -1.4 | |||
4,233,861 | - | +0.5 | |||
4,233,861 | - | +0.0 | |||
4,233,863 | + | +1.2 | |||
4,233,863 | + | +2.2 | |||
4,233,869 | - | -1.1 | |||
4,233,869 | - | +0.6 | |||
4,233,869 | - | -0.8 | |||
4,233,869 | - | -0.9 | |||
4,233,869 | - | +0.2 | |||
4,233,869 | - | -0.2 | |||
4,233,869 | - | +1.4 | |||
4,233,869 | - | -1.8 | |||
4,233,869 | - | -2.1 | |||
4,233,869 | - | +0.1 | |||
4,233,871 | - | -1.8 | |||
4,233,871 | - | +0.4 | |||
4,233,927 | - | -2.3 | |||
4,233,961 | + | yjbE | b4026 | 0.13 | +0.3 |
4,233,961 | + | yjbE | b4026 | 0.13 | -1.3 |
4,233,962 | - | yjbE | b4026 | 0.14 | -0.9 |
4,233,963 | + | yjbE | b4026 | 0.14 | -0.0 |
4,233,977 | + | yjbE | b4026 | 0.20 | +0.3 |
4,233,987 | + | yjbE | b4026 | 0.24 | +0.3 |
4,233,993 | + | yjbE | b4026 | 0.26 | +0.2 |
4,233,995 | + | yjbE | b4026 | 0.27 | -0.0 |
4,233,996 | + | yjbE | b4026 | 0.28 | -2.4 |
4,233,996 | + | yjbE | b4026 | 0.28 | -0.5 |
4,233,996 | + | yjbE | b4026 | 0.28 | -0.6 |
4,233,996 | + | yjbE | b4026 | 0.28 | -0.4 |
4,233,996 | + | yjbE | b4026 | 0.28 | -0.8 |
4,233,998 | + | yjbE | b4026 | 0.28 | -0.1 |
4,234,003 | - | yjbE | b4026 | 0.30 | -0.1 |
4,234,003 | - | yjbE | b4026 | 0.30 | -0.2 |
4,234,003 | - | yjbE | b4026 | 0.30 | +0.3 |
4,234,003 | - | yjbE | b4026 | 0.30 | +1.5 |
4,234,003 | - | yjbE | b4026 | 0.30 | -0.6 |
4,234,003 | - | yjbE | b4026 | 0.30 | -0.3 |
4,234,004 | - | yjbE | b4026 | 0.31 | -0.5 |
4,234,004 | - | yjbE | b4026 | 0.31 | -0.2 |
4,234,004 | - | yjbE | b4026 | 0.31 | -0.2 |
4,234,004 | - | yjbE | b4026 | 0.31 | -0.9 |
4,234,004 | - | yjbE | b4026 | 0.31 | -0.2 |
4,234,006 | - | yjbE | b4026 | 0.32 | -0.6 |
4,234,006 | - | yjbE | b4026 | 0.32 | +0.6 |
4,234,029 | - | yjbE | b4026 | 0.41 | -0.1 |
4,234,126 | + | yjbE | b4026 | 0.81 | -0.8 |
4,234,161 | + | +0.8 | |||
4,234,184 | - | +0.3 | |||
4,234,212 | + | +1.2 | |||
4,234,214 | + | -0.5 | |||
4,234,235 | + | -0.8 | |||
4,234,264 | - | -0.1 | |||
4,234,280 | - | -0.4 | |||
4,234,280 | - | +0.2 | |||
4,234,300 | - | -0.9 | |||
4,234,300 | - | -0.2 | |||
4,234,329 | - | +0.0 | |||
4,234,331 | + | -1.2 | |||
4,234,331 | + | -1.6 | |||
4,234,340 | + | -0.4 | |||
4,234,340 | + | +1.6 | |||
4,234,348 | - | +0.2 | |||
4,234,399 | - | yjbF | b4027 | 0.18 | +0.1 |
Or see this region's nucleotide sequence