Strain Fitness in Escherichia coli BW25113 around b4022

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
4,227,407	+				+0.6
4,227,427	+				+0.5
4,227,462	-				-0.7
4,227,509	+				+0.7
4,227,509	+				+0.9
4,227,527	-				-0.7
4,227,527	-				+0.5
4,227,534	-				-0.6
4,227,551	-	pepE	b4021	0.11	-0.2
4,227,556	-	pepE	b4021	0.12	-0.4
4,227,556	-	pepE	b4021	0.12	+0.1
4,227,621	-	pepE	b4021	0.21	+0.0
4,227,626	+	pepE	b4021	0.22	-1.3
4,227,626	+	pepE	b4021	0.22	+0.9
4,227,634	+	pepE	b4021	0.23	+0.2
4,227,634	+	pepE	b4021	0.23	-2.6
4,227,657	-	pepE	b4021	0.26	+0.1
4,227,739	+	pepE	b4021	0.38	+0.9
4,227,739	+	pepE	b4021	0.38	-1.2
4,227,772	+	pepE	b4021	0.43	-0.5
4,227,796	-	pepE	b4021	0.46	-0.1
4,227,796	-	pepE	b4021	0.46	-0.4
4,227,813	+	pepE	b4021	0.49	+0.6
4,227,821	-	pepE	b4021	0.50	+0.8
4,227,887	-	pepE	b4021	0.60	-0.4
4,227,887	-	pepE	b4021	0.60	+0.3
4,227,888	-	pepE	b4021	0.60	-0.0
4,227,888	-	pepE	b4021	0.60	+0.9
4,227,993	+	pepE	b4021	0.75	+0.7
4,228,039	-	pepE	b4021	0.82	-0.1
4,228,077	+	pepE	b4021	0.87	-0.7
4,228,087	-	pepE	b4021	0.89	+1.0
4,228,087	-	pepE	b4021	0.89	+0.2
4,228,118	-				-2.5
4,228,118	-				-1.4
4,228,119	-				-0.0
4,228,125	-				+0.2
4,228,125	-				-0.9
4,228,159	+				-1.4
4,228,162	+				-0.5
4,228,162	+				+0.7
4,228,200	+				+0.7
4,228,200	+				+0.6
4,228,223	+				+0.8
4,228,223	+				+0.6
4,228,225	+				-0.6
4,228,225	+				+0.9
4,228,225	+				+0.4
4,228,231	-				+0.0
4,228,231	-				+1.4
4,228,231	-				-1.7
4,228,235	+				+0.8
4,228,235	+				+0.1
4,228,235	+				-1.8
4,228,252	-				-0.1
4,228,267	+				+0.3
4,228,303	+				-0.1
4,228,344	-				-0.4
4,228,344	-				-0.3
4,228,369	-				-0.4
4,228,369	-				+0.4
4,228,387	+				-2.0
4,228,451	+				+0.2
4,228,451	+				-1.2
4,228,451	+				-0.5
4,228,451	+				-1.4
4,228,459	-				-1.9
4,228,459	-				-0.1
4,228,459	-				-1.3
4,228,461	+				-1.5
4,228,478	+	yjbC	b4022	0.12	-0.9
4,228,478	-	yjbC	b4022	0.12	-1.6
4,228,488	-	yjbC	b4022	0.13	-0.6
4,228,498	+	yjbC	b4022	0.14	-3.0
4,228,511	+	yjbC	b4022	0.15	-0.9
4,228,547	+	yjbC	b4022	0.19	-0.5
4,228,555	-	yjbC	b4022	0.20	-0.4
4,228,555	-	yjbC	b4022	0.20	+0.4
4,228,555	-	yjbC	b4022	0.20	-0.5
4,228,605	-	yjbC	b4022	0.26	-0.5
4,228,610	+	yjbC	b4022	0.27	-2.4
4,228,688	-	yjbC	b4022	0.36	-2.1
4,228,695	+	yjbC	b4022	0.36	-0.5
4,228,703	-	yjbC	b4022	0.37	-1.4
4,228,737	+	yjbC	b4022	0.41	+0.1
4,228,737	+	yjbC	b4022	0.41	-0.4
4,228,786	+	yjbC	b4022	0.47	-1.0
4,228,798	-	yjbC	b4022	0.48	-1.0
4,228,835	+	yjbC	b4022	0.52	-1.4
4,228,837	+	yjbC	b4022	0.53	-2.2
4,228,837	+	yjbC	b4022	0.53	+0.5
4,228,845	-	yjbC	b4022	0.54	-2.1
4,228,861	-	yjbC	b4022	0.55	-1.1
4,228,892	+	yjbC	b4022	0.59	-0.1
4,228,892	+	yjbC	b4022	0.59	-0.6
4,228,909	+	yjbC	b4022	0.61	-0.7
4,228,915	+	yjbC	b4022	0.62	-1.6
4,228,920	-	yjbC	b4022	0.62	+0.0
4,228,923	-	yjbC	b4022	0.63	+0.6
4,228,923	-	yjbC	b4022	0.63	-0.1
4,228,929	-	yjbC	b4022	0.63	-1.1
4,229,003	-	yjbC	b4022	0.72	+0.4
4,229,003	-	yjbC	b4022	0.72	-2.6
4,229,010	+	yjbC	b4022	0.73	-1.4
4,229,010	+	yjbC	b4022	0.73	-1.1
4,229,020	+	yjbC	b4022	0.74	-0.8
4,229,020	+	yjbC	b4022	0.74	-0.4
4,229,071	+	yjbC	b4022	0.79	-2.0
4,229,072	+	yjbC	b4022	0.80	-1.0
4,229,080	-	yjbC	b4022	0.81	-1.4
4,229,080	-	yjbC	b4022	0.81	-1.5
4,229,080	-	yjbC	b4022	0.81	-0.6
4,229,154	+	yjbC	b4022	0.89	-1.0
4,229,162	-				-0.1
4,229,255	+				-1.9
4,229,263	-				-2.5
4,229,263	-				+0.6
4,229,298	-				-2.9
4,229,298	-				+0.5
4,229,336	+				-0.6
4,229,421	-	yjbD	b4023	0.14	-0.1
4,229,533	+	yjbD	b4023	0.55	+0.8
4,229,539	+	yjbD	b4023	0.58	-0.2
4,229,551	+	yjbD	b4023	0.62	-0.6
4,229,555	+	yjbD	b4023	0.63	-0.6
4,229,555	+	yjbD	b4023	0.63	-0.3
4,229,563	-	yjbD	b4023	0.66	-2.4
4,229,563	-	yjbD	b4023	0.66	-0.4
4,229,563	-	yjbD	b4023	0.66	-0.8
4,229,572	+	yjbD	b4023	0.70	+0.6
4,229,583	+	yjbD	b4023	0.74	+0.8
4,229,583	+	yjbD	b4023	0.74	+1.3
4,229,591	+	yjbD	b4023	0.77	-0.0
4,229,599	+	yjbD	b4023	0.79	-1.1
4,229,603	-	yjbD	b4023	0.81	+0.9
4,229,603	-	yjbD	b4023	0.81	-0.1
4,229,609	+	yjbD	b4023	0.83	+0.8
4,229,620	+	yjbD	b4023	0.87	+0.2
4,229,638	+				+0.6
4,229,638	+				-1.1
4,229,638	+				-0.6
4,229,646	-				+0.1
4,229,648	-				+1.0
4,229,648	-				-1.1
4,229,650	+				-1.8
4,229,650	+				+0.1
4,229,650	+				+0.6
4,229,650	+				+0.6
4,229,651	+				+0.0
4,229,651	+				+0.0
4,229,659	-				+1.6
4,229,661	+				+0.3
4,229,663	+				-1.5
4,229,666	+				+1.3
4,229,668	-				-0.8
4,229,678	+				-0.6
4,229,693	-				+2.2
4,229,695	-				+2.2
4,229,734	+				-0.4
4,229,796	+				+2.2
4,229,818	+				+0.2
4,229,821	-				+0.6
4,229,826	+				-0.4
4,229,826	-				-0.4
4,229,831	-				-0.9
4,229,831	-				+1.2
4,229,831	-				+0.3
4,229,835	-				+1.1
4,229,836	-				+1.2
4,229,836	-				-1.0
4,229,836	-				-0.1
4,229,836	-				+0.3
4,229,845	+				+0.0
4,229,898	+				+0.6
4,229,898	+				+0.6
4,229,903	+				+0.6
4,229,903	+				+1.2
4,229,903	+				+0.6
4,229,910	-				+0.1
4,229,912	-				-0.9
4,229,951	-				-0.9
4,229,951	-				-0.8
4,229,953	-				+0.5
4,229,953	-				-0.1
4,229,964	-				-1.0
4,229,973	+				+0.2
4,229,973	+				+2.2
4,229,974	-				-0.5
4,229,978	+				+1.4
4,229,986	-				+0.9
4,229,986	-				-1.0
4,229,986	-				-0.6
4,229,997	-				+0.2
4,230,031	-				+0.0
4,230,031	-				-0.4
4,230,061	+	lysC	b4024	0.11	+0.5
4,230,084	-	lysC	b4024	0.13	-0.1
4,230,126	-	lysC	b4024	0.16	+0.3
4,230,182	-	lysC	b4024	0.20	+0.1
4,230,182	-	lysC	b4024	0.20	-0.5
4,230,187	-	lysC	b4024	0.21	-1.4
4,230,226	-	lysC	b4024	0.24	+0.0
4,230,226	-	lysC	b4024	0.24	+0.2
4,230,226	-	lysC	b4024	0.24	+0.4
4,230,226	-	lysC	b4024	0.24	-0.6
4,230,229	+	lysC	b4024	0.24	-1.1
4,230,237	-	lysC	b4024	0.24	-0.2