Strain Fitness in Escherichia coli BW25113 around b3835

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntubiE and yigP are separated by 13 nucleotidesyigP and ubiB overlap by 4 nucleotidesubiB and b3836 are separated by 78 nucleotidesb3836 and tatB are separated by 3 nucleotidestatB and tatC are separated by 2 nucleotides b3833: ubiE - ubiquinone/menaquinone biosynthesis methyltransferase (NCBI), at 4,016,878 to 4,017,633 ubiE b3834: yigP - hypothetical protein (NCBI), at 4,017,647 to 4,018,252 yigP b3835: ubiB - putative ubiquinone biosynthesis protein UbiB (NCBI), at 4,018,249 to 4,019,889 ubiB b3836: b3836 - orf, hypothetical protein (VIMSS), at 4,019,968 to 4,020,237 b3836 b3838: tatB - sec-independent translocase (NCBI), at 4,020,241 to 4,020,756 tatB b3839: tatC - TatABCE protein translocation system subunit (NCBI), at 4,020,759 to 4,021,535 tatC Position (kb) 4018 4019 4020Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 4017.662 kb on + strandat 4017.674 kb on + strandat 4017.674 kb on + strandat 4017.698 kb on + strandat 4017.698 kb on + strandat 4017.711 kb on + strand, within yigPat 4017.732 kb on + strand, within yigPat 4017.752 kb on + strand, within yigPat 4017.797 kb on + strand, within yigPat 4017.797 kb on + strand, within yigPat 4017.816 kb on + strand, within yigPat 4017.816 kb on + strand, within yigPat 4017.843 kb on + strand, within yigPat 4017.843 kb on + strand, within yigPat 4017.916 kb on + strand, within yigPat 4017.992 kb on + strand, within yigPat 4017.992 kb on + strand, within yigPat 4018.018 kb on + strand, within yigPat 4018.018 kb on + strand, within yigPat 4018.038 kb on + strand, within yigPat 4019.914 kb on + strandat 4019.914 kb on + strandat 4019.957 kb on - strandat 4019.960 kb on - strandat 4019.972 kb on - strandat 4019.975 kb on + strandat 4019.975 kb on + strandat 4019.981 kb on + strandat 4019.983 kb on - strandat 4019.991 kb on + strandat 4020.007 kb on - strand, within b3836at 4020.011 kb on + strand, within b3836at 4020.014 kb on + strand, within b3836at 4020.181 kb on + strand, within b3836at 4020.182 kb on + strand, within b3836at 4020.182 kb on + strand, within b3836at 4020.197 kb on + strand, within b3836at 4020.224 kb on - strandat 4020.226 kb on - strandat 4020.229 kb on + strandat 4020.232 kb on + strandat 4020.239 kb on - strandat 4020.262 kb on - strandat 4020.276 kb on + strandat 4020.282 kb on - strandat 4020.353 kb on - strand, within tatBat 4020.357 kb on + strand, within tatBat 4020.357 kb on + strand, within tatBat 4020.366 kb on + strand, within tatBat 4020.366 kb on + strand, within tatBat 4020.366 kb on + strand, within tatBat 4020.366 kb on + strand, within tatBat 4020.368 kb on + strand, within tatBat 4020.376 kb on + strand, within tatBat 4020.381 kb on + strand, within tatBat 4020.381 kb on + strand, within tatBat 4020.381 kb on + strand, within tatBat 4020.381 kb on + strand, within tatBat 4020.384 kb on - strand, within tatBat 4020.423 kb on - strand, within tatBat 4020.423 kb on - strand, within tatBat 4020.425 kb on + strand, within tatBat 4020.453 kb on + strand, within tatBat 4020.490 kb on + strand, within tatBat 4020.491 kb on + strand, within tatBat 4020.491 kb on + strand, within tatBat 4020.495 kb on + strand, within tatBat 4020.495 kb on + strand, within tatBat 4020.522 kb on + strand, within tatBat 4020.527 kb on - strand, within tatBat 4020.539 kb on + strand, within tatBat 4020.572 kb on + strand, within tatBat 4020.579 kb on - strand, within tatBat 4020.596 kb on + strand, within tatBat 4020.640 kb on - strand, within tatBat 4020.645 kb on - strand, within tatBat 4020.686 kb on + strand, within tatBat 4020.727 kb on - strandat 4020.750 kb on + strandat 4020.771 kb on - strandat 4020.777 kb on - strandat 4020.782 kb on + strandat 4020.796 kb on + strandat 4020.804 kb on - strandat 4020.814 kb on - strandat 4020.814 kb on - strandat 4020.814 kb on - strandat 4020.824 kb on - strandat 4020.839 kb on + strand, within tatCat 4020.845 kb on + strand, within tatCat 4020.851 kb on - strand, within tatCat 4020.881 kb on + strand, within tatC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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4,017,662 + -0.1
4,017,674 + -1.7
4,017,674 + +0.2
4,017,698 + -2.5
4,017,698 + -0.9
4,017,711 + yigP b3834 0.11 -0.5
4,017,732 + yigP b3834 0.14 +0.1
4,017,752 + yigP b3834 0.17 -0.3
4,017,797 + yigP b3834 0.25 -1.0
4,017,797 + yigP b3834 0.25 +1.5
4,017,816 + yigP b3834 0.28 +0.0
4,017,816 + yigP b3834 0.28 +0.6
4,017,843 + yigP b3834 0.32 +0.4
4,017,843 + yigP b3834 0.32 +0.3
4,017,916 + yigP b3834 0.44 +1.5
4,017,992 + yigP b3834 0.57 +1.6
4,017,992 + yigP b3834 0.57 -0.0
4,018,018 + yigP b3834 0.61 +0.2
4,018,018 + yigP b3834 0.61 +0.6
4,018,038 + yigP b3834 0.65 +1.6
4,019,914 + -0.5
4,019,914 + +1.1
4,019,957 - -3.0
4,019,960 - -3.3
4,019,972 - -2.6
4,019,975 + -2.5
4,019,975 + -3.0
4,019,981 + -1.7
4,019,983 - -2.7
4,019,991 + -2.1
4,020,007 - b3836 b3836 0.14 +0.2
4,020,011 + b3836 b3836 0.16 -2.7
4,020,014 + b3836 b3836 0.17 -2.0
4,020,181 + b3836 b3836 0.79 -1.5
4,020,182 + b3836 b3836 0.79 -1.0
4,020,182 + b3836 b3836 0.79 -1.5
4,020,197 + b3836 b3836 0.85 +0.1
4,020,224 - -3.9
4,020,226 - -2.9
4,020,229 + -2.3
4,020,232 + +0.3
4,020,239 - -3.1
4,020,262 - +0.2
4,020,276 + -2.3
4,020,282 - -0.8
4,020,353 - tatB b3838 0.22 -2.6
4,020,357 + tatB b3838 0.22 -1.1
4,020,357 + tatB b3838 0.22 -1.5
4,020,366 + tatB b3838 0.24 -1.4
4,020,366 + tatB b3838 0.24 -1.6
4,020,366 + tatB b3838 0.24 -0.8
4,020,366 + tatB b3838 0.24 -1.5
4,020,368 + tatB b3838 0.25 -1.1
4,020,376 + tatB b3838 0.26 -1.6
4,020,381 + tatB b3838 0.27 +0.6
4,020,381 + tatB b3838 0.27 -1.4
4,020,381 + tatB b3838 0.27 -0.4
4,020,381 + tatB b3838 0.27 +0.2
4,020,384 - tatB b3838 0.28 -0.4
4,020,423 - tatB b3838 0.35 -0.8
4,020,423 - tatB b3838 0.35 +0.6
4,020,425 + tatB b3838 0.36 -1.8
4,020,453 + tatB b3838 0.41 -2.3
4,020,490 + tatB b3838 0.48 -2.3
4,020,491 + tatB b3838 0.48 -1.5
4,020,491 + tatB b3838 0.48 -2.8
4,020,495 + tatB b3838 0.49 -1.8
4,020,495 + tatB b3838 0.49 -0.4
4,020,522 + tatB b3838 0.54 -2.0
4,020,527 - tatB b3838 0.55 -0.6
4,020,539 + tatB b3838 0.58 -2.3
4,020,572 + tatB b3838 0.64 -2.0
4,020,579 - tatB b3838 0.66 -3.8
4,020,596 + tatB b3838 0.69 -1.3
4,020,640 - tatB b3838 0.77 -2.0
4,020,645 - tatB b3838 0.78 -2.3
4,020,686 + tatB b3838 0.86 -0.7
4,020,727 - -2.0
4,020,750 + +1.4
4,020,771 - +1.2
4,020,777 - -3.2
4,020,782 + -2.3
4,020,796 + -0.4
4,020,804 - +0.2
4,020,814 - -0.4
4,020,814 - +0.2
4,020,814 - -0.6
4,020,824 - -2.6
4,020,839 + tatC b3839 0.10 -0.1
4,020,845 + tatC b3839 0.11 -1.5
4,020,851 - tatC b3839 0.12 -0.6
4,020,881 + tatC b3839 0.16 -0.4

Or see this region's nucleotide sequence