Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yidE and ibpB are separated by 195 nucleotides ibpB and ibpA are separated by 111 nucleotides ibpA and yidQ are separated by 305 nucleotides yidQ and yidR are separated by 1 nucleotides
b3685: yidE - putative transport protein (VIMSS), at 3,862,635 to 3,864,296
yidE
b3686: ibpB - heat shock chaperone (NCBI), at 3,864,492 to 3,864,920
ibpB
b3687: ibpA - heat shock chaperone (NCBI), at 3,865,032 to 3,865,445
ibpA
b3688: yidQ - orf, hypothetical protein (VIMSS), at 3,865,751 to 3,866,083
yidQ
b3689: yidR - conserved protein (NCBI), at 3,866,085 to 3,867,332
yidR
Position (kb)
3865
3866 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 3864.039 kb on + strand, within yidE at 3864.053 kb on - strand, within yidE at 3864.059 kb on + strand, within yidE at 3864.087 kb on - strand, within yidE at 3864.130 kb on - strand at 3864.140 kb on - strand at 3864.140 kb on - strand at 3864.170 kb on + strand at 3864.186 kb on - strand at 3864.186 kb on - strand at 3864.201 kb on + strand at 3864.214 kb on - strand at 3864.214 kb on - strand at 3864.239 kb on + strand at 3864.239 kb on + strand at 3864.269 kb on - strand at 3864.275 kb on + strand at 3864.283 kb on - strand at 3864.284 kb on + strand at 3864.289 kb on - strand at 3864.292 kb on - strand at 3864.292 kb on - strand at 3864.295 kb on + strand at 3864.376 kb on + strand at 3864.433 kb on + strand at 3864.488 kb on - strand at 3864.488 kb on - strand at 3864.510 kb on + strand at 3864.545 kb on + strand, within ibpB at 3864.569 kb on - strand, within ibpB at 3864.583 kb on - strand, within ibpB at 3864.626 kb on + strand, within ibpB at 3864.719 kb on + strand, within ibpB at 3864.719 kb on + strand, within ibpB at 3864.727 kb on - strand, within ibpB at 3864.727 kb on - strand, within ibpB at 3864.733 kb on + strand, within ibpB at 3864.778 kb on - strand, within ibpB at 3864.778 kb on - strand, within ibpB at 3864.778 kb on - strand, within ibpB at 3864.789 kb on - strand, within ibpB at 3864.812 kb on - strand, within ibpB at 3864.856 kb on + strand, within ibpB at 3864.864 kb on - strand, within ibpB at 3864.866 kb on + strand, within ibpB at 3864.870 kb on + strand, within ibpB at 3865.029 kb on - strand at 3865.029 kb on - strand at 3865.029 kb on - strand at 3865.090 kb on + strand, within ibpA at 3865.090 kb on + strand, within ibpA at 3865.090 kb on + strand, within ibpA at 3865.090 kb on + strand, within ibpA at 3865.090 kb on + strand, within ibpA at 3865.098 kb on - strand, within ibpA at 3865.098 kb on - strand, within ibpA at 3865.098 kb on - strand, within ibpA at 3865.112 kb on + strand, within ibpA at 3865.113 kb on - strand, within ibpA at 3865.120 kb on - strand, within ibpA at 3865.120 kb on - strand, within ibpA at 3865.120 kb on - strand, within ibpA at 3865.180 kb on + strand, within ibpA at 3865.182 kb on - strand, within ibpA at 3865.184 kb on + strand, within ibpA at 3865.210 kb on - strand, within ibpA at 3865.217 kb on + strand, within ibpA at 3865.233 kb on - strand, within ibpA at 3865.233 kb on - strand, within ibpA at 3865.240 kb on + strand, within ibpA at 3865.241 kb on + strand, within ibpA at 3865.244 kb on - strand, within ibpA at 3865.244 kb on - strand, within ibpA at 3865.253 kb on + strand, within ibpA at 3865.253 kb on - strand, within ibpA at 3865.325 kb on + strand, within ibpA at 3865.325 kb on + strand, within ibpA at 3865.330 kb on + strand, within ibpA at 3865.332 kb on + strand, within ibpA at 3865.332 kb on + strand, within ibpA at 3865.382 kb on + strand, within ibpA at 3865.389 kb on - strand, within ibpA at 3865.424 kb on - strand at 3865.428 kb on - strand at 3865.481 kb on - strand at 3865.481 kb on - strand at 3865.505 kb on + strand at 3865.613 kb on + strand at 3865.613 kb on + strand at 3865.615 kb on + strand at 3865.621 kb on - strand at 3865.630 kb on - strand at 3865.637 kb on - strand at 3865.637 kb on - strand at 3865.674 kb on - strand at 3865.711 kb on - strand at 3865.713 kb on + strand at 3865.713 kb on - strand at 3865.757 kb on + strand at 3865.763 kb on + strand at 3865.791 kb on + strand, within yidQ at 3865.799 kb on - strand, within yidQ at 3865.813 kb on + strand, within yidQ at 3865.813 kb on + strand, within yidQ at 3865.821 kb on - strand, within yidQ at 3865.821 kb on - strand, within yidQ at 3865.825 kb on + strand, within yidQ at 3865.833 kb on - strand, within yidQ at 3865.957 kb on - strand, within yidQ at 3865.969 kb on + strand, within yidQ at 3865.969 kb on + strand, within yidQ at 3865.969 kb on + strand, within yidQ at 3865.974 kb on + strand, within yidQ at 3865.974 kb on + strand, within yidQ at 3865.998 kb on + strand, within yidQ at 3866.112 kb on - strand at 3866.112 kb on - strand at 3866.124 kb on + strand at 3866.124 kb on + strand at 3866.125 kb on + strand at 3866.135 kb on + strand at 3866.135 kb on + strand at 3866.162 kb on - strand at 3866.162 kb on - strand at 3866.174 kb on - strand at 3866.237 kb on + strand, within yidR at 3866.237 kb on - strand, within yidR at 3866.308 kb on + strand, within yidR at 3866.308 kb on + strand, within yidR at 3866.320 kb on + strand, within yidR at 3866.321 kb on - strand, within yidR at 3866.321 kb on - strand, within yidR at 3866.321 kb on - strand, within yidR at 3866.323 kb on + strand, within yidR at 3866.359 kb on - strand, within yidR at 3866.359 kb on - strand, within yidR at 3866.361 kb on + strand, within yidR at 3866.373 kb on + strand, within yidR at 3866.410 kb on - strand, within yidR at 3866.441 kb on - strand, within yidR at 3866.443 kb on + strand, within yidR at 3866.443 kb on + strand, within yidR
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM remove 3,864,039 + yidE b3685 0.84 +0.5 3,864,053 - yidE b3685 0.85 +0.3 3,864,059 + yidE b3685 0.86 -0.4 3,864,087 - yidE b3685 0.87 +0.8 3,864,130 - +0.1 3,864,140 - +0.2 3,864,140 - -0.3 3,864,170 + -0.6 3,864,186 - -1.5 3,864,186 - +1.1 3,864,201 + -0.9 3,864,214 - +0.2 3,864,214 - +0.9 3,864,239 + +1.2 3,864,239 + -1.1 3,864,269 - -0.7 3,864,275 + -0.3 3,864,283 - -0.8 3,864,284 + -0.5 3,864,289 - -0.4 3,864,292 - -0.1 3,864,292 - +1.2 3,864,295 + -0.4 3,864,376 + -0.1 3,864,433 + -1.1 3,864,488 - -0.9 3,864,488 - -0.9 3,864,510 + +0.2 3,864,545 + ibpB b3686 0.12 -0.5 3,864,569 - ibpB b3686 0.18 -1.2 3,864,583 - ibpB b3686 0.21 -0.2 3,864,626 + ibpB b3686 0.31 +0.9 3,864,719 + ibpB b3686 0.53 -1.4 3,864,719 + ibpB b3686 0.53 -0.1 3,864,727 - ibpB b3686 0.55 -0.3 3,864,727 - ibpB b3686 0.55 -2.2 3,864,733 + ibpB b3686 0.56 -1.4 3,864,778 - ibpB b3686 0.67 -0.4 3,864,778 - ibpB b3686 0.67 -0.5 3,864,778 - ibpB b3686 0.67 -2.2 3,864,789 - ibpB b3686 0.69 -0.4 3,864,812 - ibpB b3686 0.75 +0.8 3,864,856 + ibpB b3686 0.85 -1.8 3,864,864 - ibpB b3686 0.87 -3.4 3,864,866 + ibpB b3686 0.87 -2.3 3,864,870 + ibpB b3686 0.88 -4.0 3,865,029 - -0.6 3,865,029 - -0.0 3,865,029 - -0.0 3,865,090 + ibpA b3687 0.14 -2.2 3,865,090 + ibpA b3687 0.14 -2.7 3,865,090 + ibpA b3687 0.14 -3.7 3,865,090 + ibpA b3687 0.14 -1.1 3,865,090 + ibpA b3687 0.14 -2.3 3,865,098 - ibpA b3687 0.16 -0.9 3,865,098 - ibpA b3687 0.16 -1.7 3,865,098 - ibpA b3687 0.16 -2.4 3,865,112 + ibpA b3687 0.19 -2.5 3,865,113 - ibpA b3687 0.20 -1.5 3,865,120 - ibpA b3687 0.21 -2.4 3,865,120 - ibpA b3687 0.21 -1.6 3,865,120 - ibpA b3687 0.21 -0.2 3,865,180 + ibpA b3687 0.36 -1.4 3,865,182 - ibpA b3687 0.36 -0.4 3,865,184 + ibpA b3687 0.37 -2.8 3,865,210 - ibpA b3687 0.43 -1.1 3,865,217 + ibpA b3687 0.45 -2.9 3,865,233 - ibpA b3687 0.49 -1.1 3,865,233 - ibpA b3687 0.49 -2.3 3,865,240 + ibpA b3687 0.50 -4.6 3,865,241 + ibpA b3687 0.50 -2.6 3,865,244 - ibpA b3687 0.51 -2.3 3,865,244 - ibpA b3687 0.51 -1.6 3,865,253 + ibpA b3687 0.53 -3.5 3,865,253 - ibpA b3687 0.53 -1.4 3,865,325 + ibpA b3687 0.71 -3.1 3,865,325 + ibpA b3687 0.71 -1.4 3,865,330 + ibpA b3687 0.72 -2.2 3,865,332 + ibpA b3687 0.72 -3.2 3,865,332 + ibpA b3687 0.72 -2.8 3,865,382 + ibpA b3687 0.85 -4.5 3,865,389 - ibpA b3687 0.86 -1.1 3,865,424 - -0.5 3,865,428 - +0.1 3,865,481 - -2.9 3,865,481 - -1.9 3,865,505 + -2.9 3,865,613 + -2.3 3,865,613 + -2.4 3,865,615 + -2.9 3,865,621 - +0.5 3,865,630 - -0.2 3,865,637 - -0.6 3,865,637 - -0.4 3,865,674 - +0.8 3,865,711 - +0.5 3,865,713 + -4.1 3,865,713 - -1.5 3,865,757 + +0.1 3,865,763 + -0.4 3,865,791 + yidQ b3688 0.12 +2.4 3,865,799 - yidQ b3688 0.14 +2.1 3,865,813 + yidQ b3688 0.19 +1.7 3,865,813 + yidQ b3688 0.19 +2.5 3,865,821 - yidQ b3688 0.21 +2.2 3,865,821 - yidQ b3688 0.21 +2.3 3,865,825 + yidQ b3688 0.22 +1.7 3,865,833 - yidQ b3688 0.25 +0.6 3,865,957 - yidQ b3688 0.62 +2.5 3,865,969 + yidQ b3688 0.65 +2.2 3,865,969 + yidQ b3688 0.65 +1.0 3,865,969 + yidQ b3688 0.65 +2.5 3,865,974 + yidQ b3688 0.67 +2.2 3,865,974 + yidQ b3688 0.67 +1.6 3,865,998 + yidQ b3688 0.74 +1.5 3,866,112 - +0.7 3,866,112 - -0.2 3,866,124 + -0.1 3,866,124 + -0.6 3,866,125 + +0.9 3,866,135 + -0.6 3,866,135 + +0.3 3,866,162 - +0.5 3,866,162 - +1.0 3,866,174 - +1.5 3,866,237 + yidR b3689 0.12 +0.4 3,866,237 - yidR b3689 0.12 -0.1 3,866,308 + yidR b3689 0.18 +0.1 3,866,308 + yidR b3689 0.18 +0.6 3,866,320 + yidR b3689 0.19 -0.0 3,866,321 - yidR b3689 0.19 -1.6 3,866,321 - yidR b3689 0.19 +0.3 3,866,321 - yidR b3689 0.19 -0.8 3,866,323 + yidR b3689 0.19 -1.4 3,866,359 - yidR b3689 0.22 -2.7 3,866,359 - yidR b3689 0.22 -2.1 3,866,361 + yidR b3689 0.22 -0.9 3,866,373 + yidR b3689 0.23 +0.4 3,866,410 - yidR b3689 0.26 +1.0 3,866,441 - yidR b3689 0.29 -0.9 3,866,443 + yidR b3689 0.29 -0.9 3,866,443 + yidR b3689 0.29 -1.7
Or see this region's nucleotide sequence