Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhhM and yhhN are separated by 140 nucleotides yhhN and zntA are separated by 73 nucleotides zntA and yhhP are separated by 101 nucleotides yhhP and yhhQ are separated by 220 nucleotides
b3467: yhhM - hypothetical protein (NCBI), at 3,603,274 to 3,603,633
yhhM
b3468: yhhN - conserved inner membrane protein (NCBI), at 3,603,774 to 3,604,400
yhhN
b3469: zntA - zinc, cobalt and lead efflux system (NCBI), at 3,604,474 to 3,606,672
zntA
b3470: yhhP - cell developmental protein SirA (NCBI), at 3,606,774 to 3,607,019
yhhP
b3471: yhhQ - conserved inner membrane protein (NCBI), at 3,607,240 to 3,607,905
yhhQ
Position (kb)
3604
3605
3606
3607 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3603.678 kb on + strand at 3603.726 kb on + strand at 3603.776 kb on + strand at 3603.812 kb on + strand at 3603.825 kb on + strand at 3603.825 kb on + strand at 3603.945 kb on + strand, within yhhN at 3603.945 kb on + strand, within yhhN at 3603.955 kb on - strand, within yhhN at 3603.960 kb on + strand, within yhhN at 3603.960 kb on + strand, within yhhN at 3603.968 kb on - strand, within yhhN at 3603.980 kb on - strand, within yhhN at 3603.980 kb on - strand, within yhhN at 3603.996 kb on + strand, within yhhN at 3603.996 kb on + strand, within yhhN at 3604.037 kb on + strand, within yhhN at 3604.037 kb on + strand, within yhhN at 3604.037 kb on + strand, within yhhN at 3604.037 kb on + strand, within yhhN at 3604.037 kb on + strand, within yhhN at 3604.045 kb on - strand, within yhhN at 3604.045 kb on - strand, within yhhN at 3604.045 kb on - strand, within yhhN at 3604.071 kb on + strand, within yhhN at 3604.071 kb on + strand, within yhhN at 3604.087 kb on + strand, within yhhN at 3604.087 kb on + strand, within yhhN at 3604.087 kb on + strand, within yhhN at 3604.087 kb on + strand, within yhhN at 3604.092 kb on - strand, within yhhN at 3604.092 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.095 kb on - strand, within yhhN at 3604.107 kb on + strand, within yhhN at 3604.107 kb on + strand, within yhhN at 3604.119 kb on + strand, within yhhN at 3604.157 kb on - strand, within yhhN at 3604.167 kb on + strand, within yhhN at 3604.167 kb on + strand, within yhhN at 3604.170 kb on - strand, within yhhN at 3604.170 kb on - strand, within yhhN at 3604.193 kb on + strand, within yhhN at 3604.193 kb on + strand, within yhhN at 3604.193 kb on + strand, within yhhN at 3604.199 kb on - strand, within yhhN at 3604.229 kb on - strand, within yhhN at 3604.229 kb on - strand, within yhhN at 3604.360 kb on - strand at 3604.387 kb on + strand at 3604.392 kb on - strand at 3604.392 kb on - strand at 3604.423 kb on - strand at 3604.549 kb on + strand at 3604.586 kb on + strand at 3604.594 kb on - strand at 3604.594 kb on - strand at 3604.594 kb on - strand at 3604.639 kb on + strand at 3604.716 kb on + strand, within zntA at 3604.716 kb on + strand, within zntA at 3604.780 kb on + strand, within zntA at 3604.808 kb on + strand, within zntA at 3604.808 kb on + strand, within zntA at 3604.973 kb on - strand, within zntA at 3604.973 kb on - strand, within zntA at 3604.999 kb on + strand, within zntA at 3605.025 kb on - strand, within zntA at 3605.025 kb on - strand, within zntA at 3605.049 kb on - strand, within zntA at 3605.049 kb on - strand, within zntA at 3605.058 kb on + strand, within zntA at 3605.202 kb on - strand, within zntA at 3605.207 kb on - strand, within zntA at 3605.207 kb on - strand, within zntA at 3605.298 kb on + strand, within zntA at 3605.307 kb on - strand, within zntA at 3605.368 kb on + strand, within zntA at 3605.403 kb on - strand, within zntA at 3605.403 kb on - strand, within zntA at 3605.404 kb on + strand, within zntA at 3605.404 kb on + strand, within zntA at 3605.413 kb on - strand, within zntA at 3605.413 kb on - strand, within zntA at 3605.414 kb on + strand, within zntA at 3605.426 kb on + strand, within zntA at 3605.426 kb on + strand, within zntA at 3605.427 kb on + strand, within zntA at 3605.466 kb on - strand, within zntA at 3605.495 kb on + strand, within zntA at 3605.517 kb on - strand, within zntA at 3605.529 kb on + strand, within zntA at 3605.530 kb on + strand, within zntA at 3605.613 kb on + strand, within zntA at 3605.697 kb on + strand, within zntA at 3605.712 kb on + strand, within zntA at 3605.759 kb on - strand, within zntA at 3605.759 kb on - strand, within zntA at 3605.846 kb on - strand, within zntA at 3605.849 kb on + strand, within zntA at 3605.870 kb on - strand, within zntA at 3605.905 kb on + strand, within zntA at 3605.905 kb on + strand, within zntA at 3605.909 kb on + strand, within zntA at 3605.932 kb on - strand, within zntA at 3606.008 kb on - strand, within zntA at 3606.008 kb on - strand, within zntA at 3606.008 kb on - strand, within zntA at 3606.021 kb on + strand, within zntA at 3606.069 kb on - strand, within zntA at 3606.074 kb on - strand, within zntA at 3606.078 kb on + strand, within zntA at 3606.100 kb on + strand, within zntA at 3606.100 kb on + strand, within zntA at 3606.144 kb on + strand, within zntA at 3606.198 kb on + strand, within zntA at 3606.203 kb on + strand, within zntA at 3606.222 kb on - strand, within zntA at 3606.298 kb on + strand, within zntA at 3606.326 kb on + strand, within zntA at 3606.331 kb on + strand, within zntA at 3606.350 kb on - strand, within zntA at 3606.350 kb on - strand, within zntA at 3606.367 kb on - strand, within zntA at 3606.367 kb on - strand, within zntA at 3606.367 kb on - strand, within zntA at 3606.383 kb on - strand, within zntA at 3606.383 kb on - strand, within zntA at 3606.414 kb on + strand, within zntA at 3606.414 kb on + strand, within zntA at 3606.448 kb on + strand, within zntA at 3606.448 kb on + strand, within zntA at 3606.454 kb on + strand at 3606.454 kb on + strand at 3606.468 kb on + strand at 3606.478 kb on + strand at 3606.478 kb on + strand at 3606.590 kb on + strand at 3606.602 kb on - strand at 3606.629 kb on - strand at 3606.631 kb on - strand at 3606.636 kb on - strand at 3606.667 kb on + strand at 3606.713 kb on - strand at 3606.713 kb on - strand at 3606.723 kb on - strand at 3606.723 kb on - strand at 3606.723 kb on - strand at 3606.727 kb on + strand at 3606.727 kb on + strand at 3606.727 kb on + strand at 3606.735 kb on - strand at 3606.735 kb on - strand at 3606.735 kb on - strand at 3606.736 kb on - strand at 3606.775 kb on - strand at 3606.775 kb on - strand at 3606.987 kb on + strand, within yhhP at 3606.997 kb on + strand at 3607.058 kb on - strand at 3607.059 kb on - strand at 3607.175 kb on - strand at 3607.177 kb on + strand at 3607.177 kb on + strand at 3607.214 kb on - strand at 3607.274 kb on - strand at 3607.274 kb on - strand at 3607.411 kb on - strand, within yhhQ at 3607.435 kb on + strand, within yhhQ at 3607.447 kb on + strand, within yhhQ at 3607.466 kb on + strand, within yhhQ at 3607.466 kb on + strand, within yhhQ at 3607.482 kb on - strand, within yhhQ at 3607.511 kb on - strand, within yhhQ at 3607.542 kb on + strand, within yhhQ at 3607.603 kb on + strand, within yhhQ at 3607.603 kb on + strand, within yhhQ at 3607.660 kb on - strand, within yhhQ at 3607.660 kb on - strand, within yhhQ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM remove 3,603,678 + -0.1 3,603,726 + +0.1 3,603,776 + -0.3 3,603,812 + -0.3 3,603,825 + -0.7 3,603,825 + +0.4 3,603,945 + yhhN b3468 0.27 -0.7 3,603,945 + yhhN b3468 0.27 +1.4 3,603,955 - yhhN b3468 0.29 +0.4 3,603,960 + yhhN b3468 0.30 +0.3 3,603,960 + yhhN b3468 0.30 -0.4 3,603,968 - yhhN b3468 0.31 -0.2 3,603,980 - yhhN b3468 0.33 -0.2 3,603,980 - yhhN b3468 0.33 +1.0 3,603,996 + yhhN b3468 0.35 -1.1 3,603,996 + yhhN b3468 0.35 +0.4 3,604,037 + yhhN b3468 0.42 -0.3 3,604,037 + yhhN b3468 0.42 -0.3 3,604,037 + yhhN b3468 0.42 +1.2 3,604,037 + yhhN b3468 0.42 +1.3 3,604,037 + yhhN b3468 0.42 -0.2 3,604,045 - yhhN b3468 0.43 +0.5 3,604,045 - yhhN b3468 0.43 +0.5 3,604,045 - yhhN b3468 0.43 +0.4 3,604,071 + yhhN b3468 0.47 -1.5 3,604,071 + yhhN b3468 0.47 -0.8 3,604,087 + yhhN b3468 0.50 -0.1 3,604,087 + yhhN b3468 0.50 +0.5 3,604,087 + yhhN b3468 0.50 -0.1 3,604,087 + yhhN b3468 0.50 -0.1 3,604,092 - yhhN b3468 0.51 -0.4 3,604,092 - yhhN b3468 0.51 +0.6 3,604,095 - yhhN b3468 0.51 -0.2 3,604,095 - yhhN b3468 0.51 -0.1 3,604,095 - yhhN b3468 0.51 +0.5 3,604,095 - yhhN b3468 0.51 -0.2 3,604,095 - yhhN b3468 0.51 +0.4 3,604,095 - yhhN b3468 0.51 +1.6 3,604,095 - yhhN b3468 0.51 +0.5 3,604,107 + yhhN b3468 0.53 -1.1 3,604,107 + yhhN b3468 0.53 +0.1 3,604,119 + yhhN b3468 0.55 +0.4 3,604,157 - yhhN b3468 0.61 +0.2 3,604,167 + yhhN b3468 0.63 +0.2 3,604,167 + yhhN b3468 0.63 +0.3 3,604,170 - yhhN b3468 0.63 +0.6 3,604,170 - yhhN b3468 0.63 +1.3 3,604,193 + yhhN b3468 0.67 +0.4 3,604,193 + yhhN b3468 0.67 +0.5 3,604,193 + yhhN b3468 0.67 -0.1 3,604,199 - yhhN b3468 0.68 +1.7 3,604,229 - yhhN b3468 0.73 +0.8 3,604,229 - yhhN b3468 0.73 -0.6 3,604,360 - +0.4 3,604,387 + +0.7 3,604,392 - +0.4 3,604,392 - +2.3 3,604,423 - -0.1 3,604,549 + -0.1 3,604,586 + -0.3 3,604,594 - -0.9 3,604,594 - +0.3 3,604,594 - -0.6 3,604,639 + +0.4 3,604,716 + zntA b3469 0.11 +0.7 3,604,716 + zntA b3469 0.11 +1.3 3,604,780 + zntA b3469 0.14 -2.3 3,604,808 + zntA b3469 0.15 -0.9 3,604,808 + zntA b3469 0.15 -0.9 3,604,973 - zntA b3469 0.23 +2.3 3,604,973 - zntA b3469 0.23 -1.6 3,604,999 + zntA b3469 0.24 -0.7 3,605,025 - zntA b3469 0.25 -0.1 3,605,025 - zntA b3469 0.25 -0.3 3,605,049 - zntA b3469 0.26 -0.6 3,605,049 - zntA b3469 0.26 +0.7 3,605,058 + zntA b3469 0.27 +1.3 3,605,202 - zntA b3469 0.33 -0.6 3,605,207 - zntA b3469 0.33 -1.1 3,605,207 - zntA b3469 0.33 +0.7 3,605,298 + zntA b3469 0.37 -2.4 3,605,307 - zntA b3469 0.38 +0.7 3,605,368 + zntA b3469 0.41 -0.8 3,605,403 - zntA b3469 0.42 -0.1 3,605,403 - zntA b3469 0.42 +0.3 3,605,404 + zntA b3469 0.42 +1.3 3,605,404 + zntA b3469 0.42 +0.3 3,605,413 - zntA b3469 0.43 -0.7 3,605,413 - zntA b3469 0.43 -0.3 3,605,414 + zntA b3469 0.43 +2.3 3,605,426 + zntA b3469 0.43 +0.3 3,605,426 + zntA b3469 0.43 -0.1 3,605,427 + zntA b3469 0.43 -1.1 3,605,466 - zntA b3469 0.45 +0.9 3,605,495 + zntA b3469 0.46 -0.1 3,605,517 - zntA b3469 0.47 -1.6 3,605,529 + zntA b3469 0.48 +0.3 3,605,530 + zntA b3469 0.48 -0.6 3,605,613 + zntA b3469 0.52 +1.3 3,605,697 + zntA b3469 0.56 +0.3 3,605,712 + zntA b3469 0.56 +0.7 3,605,759 - zntA b3469 0.58 -0.7 3,605,759 - zntA b3469 0.58 -2.3 3,605,846 - zntA b3469 0.62 -0.1 3,605,849 + zntA b3469 0.63 +0.7 3,605,870 - zntA b3469 0.63 -0.7 3,605,905 + zntA b3469 0.65 -0.1 3,605,905 + zntA b3469 0.65 -0.9 3,605,909 + zntA b3469 0.65 -0.9 3,605,932 - zntA b3469 0.66 -1.3 3,606,008 - zntA b3469 0.70 +0.7 3,606,008 - zntA b3469 0.70 +0.7 3,606,008 - zntA b3469 0.70 +0.7 3,606,021 + zntA b3469 0.70 +0.7 3,606,069 - zntA b3469 0.73 -2.6 3,606,074 - zntA b3469 0.73 -1.2 3,606,078 + zntA b3469 0.73 +2.3 3,606,100 + zntA b3469 0.74 +0.7 3,606,100 + zntA b3469 0.74 -0.1 3,606,144 + zntA b3469 0.76 +0.4 3,606,198 + zntA b3469 0.78 -0.2 3,606,203 + zntA b3469 0.79 +0.3 3,606,222 - zntA b3469 0.79 -1.6 3,606,298 + zntA b3469 0.83 +1.3 3,606,326 + zntA b3469 0.84 -0.6 3,606,331 + zntA b3469 0.84 -0.5 3,606,350 - zntA b3469 0.85 +0.7 3,606,350 - zntA b3469 0.85 -0.1 3,606,367 - zntA b3469 0.86 -0.6 3,606,367 - zntA b3469 0.86 +1.3 3,606,367 - zntA b3469 0.86 +2.3 3,606,383 - zntA b3469 0.87 -0.1 3,606,383 - zntA b3469 0.87 +1.3 3,606,414 + zntA b3469 0.88 +2.3 3,606,414 + zntA b3469 0.88 -0.1 3,606,448 + zntA b3469 0.90 +0.4 3,606,448 + zntA b3469 0.90 +1.1 3,606,454 + +0.7 3,606,454 + +0.3 3,606,468 + -0.3 3,606,478 + -0.7 3,606,478 + +1.3 3,606,590 + -0.6 3,606,602 - +1.0 3,606,629 - -0.6 3,606,631 - -1.6 3,606,636 - -1.1 3,606,667 + -0.7 3,606,713 - +0.2 3,606,713 - +0.3 3,606,723 - +0.1 3,606,723 - -0.6 3,606,723 - -0.9 3,606,727 + -0.3 3,606,727 + +0.9 3,606,727 + -1.0 3,606,735 - -0.1 3,606,735 - +0.4 3,606,735 - -0.7 3,606,736 - -1.1 3,606,775 - +0.6 3,606,775 - -0.5 3,606,987 + yhhP b3470 0.87 +0.7 3,606,997 + -0.6 3,607,058 - +1.6 3,607,059 - -0.2 3,607,175 - +0.2 3,607,177 + -0.8 3,607,177 + -0.1 3,607,214 - -0.8 3,607,274 - +1.0 3,607,274 - +0.3 3,607,411 - yhhQ b3471 0.26 -1.1 3,607,435 + yhhQ b3471 0.29 +0.3 3,607,447 + yhhQ b3471 0.31 +0.1 3,607,466 + yhhQ b3471 0.34 +0.8 3,607,466 + yhhQ b3471 0.34 +0.3 3,607,482 - yhhQ b3471 0.36 -0.6 3,607,511 - yhhQ b3471 0.41 +1.4 3,607,542 + yhhQ b3471 0.45 -1.3 3,607,603 + yhhQ b3471 0.55 -0.3 3,607,603 + yhhQ b3471 0.55 +1.5 3,607,660 - yhhQ b3471 0.63 +0.3 3,607,660 - yhhQ b3471 0.63 +0.7
Or see this region's nucleotide sequence