Strain Fitness in Escherichia coli BW25113 around b2141

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntdusC and yohJ are separated by 238 nucleotidesyohJ and yohK overlap by 4 nucleotidesyohK and cdd are separated by 129 nucleotides b2140: dusC - tRNA-dihydrouridine synthase C (NCBI), at 2,227,460 to 2,228,407 dusC b2141: yohJ - hypothetical protein (NCBI), at 2,228,646 to 2,229,044 yohJ b2142: yohK - predicted inner membrane protein (NCBI), at 2,229,041 to 2,229,736 yohK b2143: cdd - cytidine deaminase (NCBI), at 2,229,866 to 2,230,750 cdd Position (kb) 2228 2229 2230Strain fitness (log2 ratio) -2 -1 0 1at 2227.663 kb on + strand, within dusCat 2227.663 kb on + strand, within dusCat 2227.671 kb on - strand, within dusCat 2227.671 kb on - strand, within dusCat 2227.671 kb on - strand, within dusCat 2227.671 kb on - strand, within dusCat 2227.671 kb on - strand, within dusCat 2227.674 kb on + strand, within dusCat 2227.675 kb on - strand, within dusCat 2227.676 kb on - strand, within dusCat 2227.747 kb on - strand, within dusCat 2227.752 kb on - strand, within dusCat 2227.752 kb on - strand, within dusCat 2227.767 kb on + strand, within dusCat 2227.767 kb on + strand, within dusCat 2227.791 kb on - strand, within dusCat 2227.791 kb on - strand, within dusCat 2227.791 kb on - strand, within dusCat 2227.808 kb on - strand, within dusCat 2227.808 kb on - strand, within dusCat 2227.862 kb on + strand, within dusCat 2227.862 kb on + strand, within dusCat 2227.891 kb on - strand, within dusCat 2227.918 kb on - strand, within dusCat 2228.138 kb on + strand, within dusCat 2228.327 kb on - strandat 2228.444 kb on - strandat 2228.444 kb on - strandat 2228.446 kb on - strandat 2228.446 kb on - strandat 2228.530 kb on - strandat 2228.542 kb on - strandat 2228.542 kb on - strandat 2228.637 kb on - strandat 2228.637 kb on - strandat 2228.716 kb on - strand, within yohJat 2228.716 kb on - strand, within yohJat 2228.753 kb on + strand, within yohJat 2228.753 kb on + strand, within yohJat 2228.951 kb on + strand, within yohJat 2229.015 kb on + strandat 2229.068 kb on - strandat 2229.123 kb on - strand, within yohKat 2229.123 kb on - strand, within yohKat 2229.123 kb on - strand, within yohKat 2229.123 kb on - strand, within yohKat 2229.190 kb on + strand, within yohKat 2229.221 kb on + strand, within yohKat 2229.221 kb on + strand, within yohKat 2229.259 kb on + strand, within yohKat 2229.272 kb on - strand, within yohKat 2229.279 kb on + strand, within yohKat 2229.279 kb on + strand, within yohKat 2229.284 kb on + strand, within yohKat 2229.284 kb on + strand, within yohKat 2229.292 kb on - strand, within yohKat 2229.525 kb on + strand, within yohKat 2229.580 kb on + strand, within yohKat 2229.588 kb on - strand, within yohKat 2229.672 kb on - strandat 2229.728 kb on - strandat 2229.728 kb on - strandat 2229.972 kb on - strand, within cddat 2229.972 kb on - strand, within cddat 2229.984 kb on - strand, within cdd

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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2,227,663 + dusC b2140 0.21 +1.7
2,227,663 + dusC b2140 0.21 +1.7
2,227,671 - dusC b2140 0.22 +0.1
2,227,671 - dusC b2140 0.22 +0.2
2,227,671 - dusC b2140 0.22 +0.2
2,227,671 - dusC b2140 0.22 +0.0
2,227,671 - dusC b2140 0.22 +0.2
2,227,674 + dusC b2140 0.23 +1.7
2,227,675 - dusC b2140 0.23 -0.7
2,227,676 - dusC b2140 0.23 +0.6
2,227,747 - dusC b2140 0.30 -0.1
2,227,752 - dusC b2140 0.31 -1.2
2,227,752 - dusC b2140 0.31 +0.4
2,227,767 + dusC b2140 0.32 -0.9
2,227,767 + dusC b2140 0.32 -0.9
2,227,791 - dusC b2140 0.35 -0.5
2,227,791 - dusC b2140 0.35 -0.4
2,227,791 - dusC b2140 0.35 -1.1
2,227,808 - dusC b2140 0.37 +0.9
2,227,808 - dusC b2140 0.37 -0.9
2,227,862 + dusC b2140 0.42 -0.9
2,227,862 + dusC b2140 0.42 -0.8
2,227,891 - dusC b2140 0.45 -0.1
2,227,918 - dusC b2140 0.48 -0.1
2,228,138 + dusC b2140 0.72 +0.2
2,228,327 - +0.6
2,228,444 - +0.0
2,228,444 - -2.0
2,228,446 - -1.5
2,228,446 - -0.1
2,228,530 - -0.1
2,228,542 - +0.6
2,228,542 - -1.3
2,228,637 - -0.7
2,228,637 - -0.1
2,228,716 - yohJ b2141 0.18 -1.1
2,228,716 - yohJ b2141 0.18 +0.3
2,228,753 + yohJ b2141 0.27 -1.3
2,228,753 + yohJ b2141 0.27 -2.2
2,228,951 + yohJ b2141 0.76 -0.9
2,229,015 + -1.3
2,229,068 - -0.4
2,229,123 - yohK b2142 0.12 +1.1
2,229,123 - yohK b2142 0.12 -0.3
2,229,123 - yohK b2142 0.12 -1.4
2,229,123 - yohK b2142 0.12 -0.4
2,229,190 + yohK b2142 0.21 +0.6
2,229,221 + yohK b2142 0.26 +1.2
2,229,221 + yohK b2142 0.26 -0.6
2,229,259 + yohK b2142 0.31 -0.7
2,229,272 - yohK b2142 0.33 +0.3
2,229,279 + yohK b2142 0.34 +1.0
2,229,279 + yohK b2142 0.34 -0.1
2,229,284 + yohK b2142 0.35 -0.9
2,229,284 + yohK b2142 0.35 -1.1
2,229,292 - yohK b2142 0.36 -2.5
2,229,525 + yohK b2142 0.70 -1.1
2,229,580 + yohK b2142 0.77 +0.0
2,229,588 - yohK b2142 0.79 +0.3
2,229,672 - -0.6
2,229,728 - +0.8
2,229,728 - -0.1
2,229,972 - cdd b2143 0.12 -1.7
2,229,972 - cdd b2143 0.12 +0.6
2,229,984 - cdd b2143 0.13 +0.1

Or see this region's nucleotide sequence