Strain Fitness in Escherichia coli BW25113 around b1953

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrcsA and dsrB are separated by 43 nucleotidesdsrB and b1953 are separated by 162 nucleotidesb1953 and dsrA are separated by 13 nucleotidesdsrA and yedP are separated by 197 nucleotides b1951: rcsA - DNA-binding transcriptional activator, co-regulator with RcsB (NCBI), at 2,021,992 to 2,022,615 rcsA b1952: dsrB - hypothetical protein (NCBI), at 2,022,659 to 2,022,847 dsrB b1953: b1953 - orf, hypothetical protein (VIMSS), at 2,023,010 to 2,023,237 b1953 b1954: dsrA - regulatory antisense RNA (NCBI), at 2,023,251 to 2,023,337 dsrA b1955: yedP - hypothetical protein (NCBI), at 2,023,535 to 2,024,350 yedP Position (kb) 2023 2024Strain fitness (log2 ratio) -1 0 1at 2022.049 kb on + strandat 2022.191 kb on + strand, within rcsAat 2022.382 kb on - strand, within rcsAat 2022.382 kb on - strand, within rcsAat 2022.413 kb on - strand, within rcsAat 2022.413 kb on - strand, within rcsAat 2022.424 kb on - strand, within rcsAat 2022.424 kb on - strand, within rcsAat 2022.427 kb on - strand, within rcsAat 2022.435 kb on + strand, within rcsAat 2022.453 kb on - strand, within rcsAat 2022.456 kb on - strand, within rcsAat 2022.610 kb on + strandat 2022.610 kb on + strandat 2022.627 kb on - strandat 2022.637 kb on - strandat 2022.650 kb on - strandat 2022.650 kb on - strandat 2022.674 kb on - strandat 2022.674 kb on - strandat 2022.694 kb on + strand, within dsrBat 2022.694 kb on + strand, within dsrBat 2022.720 kb on - strand, within dsrBat 2022.770 kb on + strand, within dsrBat 2022.770 kb on + strand, within dsrBat 2022.787 kb on - strand, within dsrBat 2022.787 kb on - strand, within dsrBat 2022.790 kb on + strand, within dsrBat 2022.792 kb on - strand, within dsrBat 2022.874 kb on + strandat 2022.874 kb on + strandat 2022.874 kb on + strandat 2022.940 kb on + strandat 2022.942 kb on + strandat 2023.038 kb on - strand, within b1953at 2023.107 kb on + strand, within b1953at 2023.138 kb on + strand, within b1953at 2023.138 kb on + strand, within b1953at 2023.138 kb on + strand, within b1953at 2023.267 kb on - strand, within dsrAat 2023.298 kb on + strand, within dsrAat 2023.343 kb on - strandat 2023.343 kb on - strandat 2023.439 kb on - strandat 2023.439 kb on - strandat 2023.517 kb on + strandat 2023.525 kb on - strandat 2023.525 kb on - strandat 2023.540 kb on - strandat 2023.540 kb on - strandat 2023.604 kb on - strandat 2023.631 kb on - strand, within yedPat 2023.767 kb on - strand, within yedPat 2024.110 kb on + strand, within yedPat 2024.134 kb on + strand, within yedPat 2024.165 kb on + strand, within yedP

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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2,022,049 + -1.0
2,022,191 + rcsA b1951 0.32 +1.7
2,022,382 - rcsA b1951 0.62 +1.7
2,022,382 - rcsA b1951 0.62 +0.7
2,022,413 - rcsA b1951 0.67 +0.7
2,022,413 - rcsA b1951 0.67 +1.7
2,022,424 - rcsA b1951 0.69 +1.7
2,022,424 - rcsA b1951 0.69 +0.1
2,022,427 - rcsA b1951 0.70 +1.7
2,022,435 + rcsA b1951 0.71 +1.7
2,022,453 - rcsA b1951 0.74 +0.4
2,022,456 - rcsA b1951 0.74 +0.7
2,022,610 + +1.7
2,022,610 + +0.5
2,022,627 - -0.2
2,022,637 - -0.5
2,022,650 - +0.5
2,022,650 - +1.1
2,022,674 - +0.1
2,022,674 - -0.3
2,022,694 + dsrB b1952 0.19 -1.5
2,022,694 + dsrB b1952 0.19 -0.3
2,022,720 - dsrB b1952 0.32 -0.0
2,022,770 + dsrB b1952 0.59 +0.1
2,022,770 + dsrB b1952 0.59 +0.3
2,022,787 - dsrB b1952 0.68 -1.0
2,022,787 - dsrB b1952 0.68 -0.4
2,022,790 + dsrB b1952 0.69 +0.2
2,022,792 - dsrB b1952 0.70 +0.3
2,022,874 + -0.4
2,022,874 + +0.9
2,022,874 + +1.2
2,022,940 + -0.8
2,022,942 + +1.2
2,023,038 - b1953 b1953 0.12 +0.1
2,023,107 + b1953 b1953 0.43 -0.2
2,023,138 + b1953 b1953 0.56 -0.6
2,023,138 + b1953 b1953 0.56 +1.4
2,023,138 + b1953 b1953 0.56 +1.7
2,023,267 - dsrA b1954 0.18 -0.1
2,023,298 + dsrA b1954 0.54 -1.4
2,023,343 - -1.7
2,023,343 - -0.1
2,023,439 - +0.1
2,023,439 - -0.6
2,023,517 + -1.1
2,023,525 - -0.1
2,023,525 - -0.6
2,023,540 - -0.1
2,023,540 - +0.3
2,023,604 - -0.8
2,023,631 - yedP b1955 0.12 +0.5
2,023,767 - yedP b1955 0.28 +1.2
2,024,110 + yedP b1955 0.70 -0.3
2,024,134 + yedP b1955 0.73 -0.1
2,024,165 + yedP b1955 0.77 -1.4

Or see this region's nucleotide sequence