Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeaU and yeaV are separated by 190 nucleotides yeaV and yeaW are separated by 31 nucleotides
b1800: yeaU - predicted dehydrogenase (NCBI), at 1,879,936 to 1,881,021
yeaU
b1801: yeaV - predicted transporter (NCBI), at 1,881,212 to 1,882,657
yeaV
b1802: yeaW - predicted 2Fe-2S cluster-containing protein (NCBI), at 1,882,689 to 1,883,813
yeaW
Position (kb)
1881
1882
1883 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1880.373 kb on + strand, within yeaU at 1880.488 kb on + strand, within yeaU at 1880.488 kb on + strand, within yeaU at 1880.489 kb on - strand, within yeaU at 1880.516 kb on + strand, within yeaU at 1880.524 kb on - strand, within yeaU at 1880.535 kb on + strand, within yeaU at 1880.628 kb on - strand, within yeaU at 1880.642 kb on + strand, within yeaU at 1880.706 kb on + strand, within yeaU at 1880.787 kb on - strand, within yeaU at 1880.846 kb on + strand, within yeaU at 1880.881 kb on + strand, within yeaU at 1880.881 kb on + strand, within yeaU at 1880.890 kb on + strand, within yeaU at 1880.904 kb on - strand, within yeaU at 1880.929 kb on - strand at 1880.953 kb on + strand at 1880.956 kb on + strand at 1880.956 kb on + strand at 1880.977 kb on - strand at 1881.022 kb on + strand at 1881.053 kb on + strand at 1881.081 kb on + strand at 1881.172 kb on + strand at 1881.192 kb on - strand at 1881.206 kb on - strand at 1881.208 kb on - strand at 1881.243 kb on - strand at 1881.260 kb on - strand at 1881.298 kb on - strand at 1881.298 kb on - strand at 1881.303 kb on - strand at 1881.307 kb on + strand at 1881.307 kb on + strand at 1881.322 kb on + strand at 1881.329 kb on + strand at 1881.474 kb on - strand, within yeaV at 1881.494 kb on - strand, within yeaV at 1881.494 kb on - strand, within yeaV at 1881.513 kb on - strand, within yeaV at 1881.715 kb on - strand, within yeaV at 1882.003 kb on + strand, within yeaV at 1882.003 kb on + strand, within yeaV at 1882.028 kb on - strand, within yeaV at 1882.190 kb on + strand, within yeaV at 1882.315 kb on + strand, within yeaV at 1882.315 kb on + strand, within yeaV at 1882.323 kb on - strand, within yeaV at 1882.323 kb on - strand, within yeaV at 1882.323 kb on - strand, within yeaV at 1882.324 kb on - strand, within yeaV at 1882.481 kb on + strand, within yeaV at 1882.635 kb on - strand at 1882.679 kb on + strand at 1882.679 kb on + strand at 1882.769 kb on + strand at 1883.061 kb on + strand, within yeaW at 1883.116 kb on - strand, within yeaW at 1883.116 kb on - strand, within yeaW at 1883.298 kb on - strand, within yeaW at 1883.298 kb on - strand, within yeaW at 1883.458 kb on - strand, within yeaW at 1883.503 kb on + strand, within yeaW at 1883.656 kb on - strand, within yeaW
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM remove 1,880,373 + yeaU b1800 0.40 -0.6 1,880,488 + yeaU b1800 0.51 +0.7 1,880,488 + yeaU b1800 0.51 -0.8 1,880,489 - yeaU b1800 0.51 -0.3 1,880,516 + yeaU b1800 0.53 +1.6 1,880,524 - yeaU b1800 0.54 -0.2 1,880,535 + yeaU b1800 0.55 +0.1 1,880,628 - yeaU b1800 0.64 -0.2 1,880,642 + yeaU b1800 0.65 +0.9 1,880,706 + yeaU b1800 0.71 +0.6 1,880,787 - yeaU b1800 0.78 +2.3 1,880,846 + yeaU b1800 0.84 +0.2 1,880,881 + yeaU b1800 0.87 -1.2 1,880,881 + yeaU b1800 0.87 +1.2 1,880,890 + yeaU b1800 0.88 -2.0 1,880,904 - yeaU b1800 0.89 +0.1 1,880,929 - +0.7 1,880,953 + -0.2 1,880,956 + -0.0 1,880,956 + +0.3 1,880,977 - +1.7 1,881,022 + -0.0 1,881,053 + -0.3 1,881,081 + +1.7 1,881,172 + +1.3 1,881,192 - -0.7 1,881,206 - -0.3 1,881,208 - -0.8 1,881,243 - +0.1 1,881,260 - +0.4 1,881,298 - +0.4 1,881,298 - -0.3 1,881,303 - +0.8 1,881,307 + +0.7 1,881,307 + +1.7 1,881,322 + +1.7 1,881,329 + +1.7 1,881,474 - yeaV b1801 0.18 +1.1 1,881,494 - yeaV b1801 0.20 +0.1 1,881,494 - yeaV b1801 0.20 +0.7 1,881,513 - yeaV b1801 0.21 -0.4 1,881,715 - yeaV b1801 0.35 -0.6 1,882,003 + yeaV b1801 0.55 +0.4 1,882,003 + yeaV b1801 0.55 -0.8 1,882,028 - yeaV b1801 0.56 -0.1 1,882,190 + yeaV b1801 0.68 +0.4 1,882,315 + yeaV b1801 0.76 +0.1 1,882,315 + yeaV b1801 0.76 +0.1 1,882,323 - yeaV b1801 0.77 -0.5 1,882,323 - yeaV b1801 0.77 -1.1 1,882,323 - yeaV b1801 0.77 -1.1 1,882,324 - yeaV b1801 0.77 -1.2 1,882,481 + yeaV b1801 0.88 +0.2 1,882,635 - -0.2 1,882,679 + +1.7 1,882,679 + -0.3 1,882,769 + -0.0 1,883,061 + yeaW b1802 0.33 +0.5 1,883,116 - yeaW b1802 0.38 -0.2 1,883,116 - yeaW b1802 0.38 -0.0 1,883,298 - yeaW b1802 0.54 -1.9 1,883,298 - yeaW b1802 0.54 -0.2 1,883,458 - yeaW b1802 0.68 +1.2 1,883,503 + yeaW b1802 0.72 -0.9 1,883,656 - yeaW b1802 0.86 -0.6
Or see this region's nucleotide sequence