Strain Fitness in Escherichia coli BW25113 around b1123

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycfZ and ymfA overlap by 4 nucleotidesymfA and potD are separated by 57 nucleotidespotD and potC overlap by 4 nucleotidespotC and potB overlap by 4 nucleotides b1121: ycfZ - predicted inner membrane protein (NCBI), at 1,179,702 to 1,180,490 ycfZ b1122: ymfA - orf, hypothetical protein (VIMSS), at 1,180,487 to 1,180,948 ymfA b1123: potD - spermidine/putrescine ABC transporter periplasmic substrate-binding protein (NCBI), at 1,181,006 to 1,182,052 potD b1124: potC - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,049 to 1,182,843 potC b1125: potB - spermidine/putrescine ABC transporter membrane protein (NCBI), at 1,182,840 to 1,183,667 potB Position (kb) 1181 1182 1183Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 1180.234 kb on - strand, within ycfZat 1180.389 kb on - strand, within ycfZat 1180.389 kb on - strand, within ycfZat 1180.438 kb on + strandat 1180.457 kb on + strandat 1180.739 kb on - strand, within ymfAat 1180.755 kb on - strand, within ymfAat 1180.755 kb on - strand, within ymfAat 1180.763 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.777 kb on - strand, within ymfAat 1180.778 kb on - strand, within ymfAat 1180.796 kb on + strand, within ymfAat 1180.796 kb on + strand, within ymfAat 1180.799 kb on - strand, within ymfAat 1180.800 kb on - strand, within ymfAat 1180.803 kb on - strand, within ymfAat 1180.809 kb on + strand, within ymfAat 1180.813 kb on + strand, within ymfAat 1180.817 kb on - strand, within ymfAat 1180.834 kb on - strand, within ymfAat 1180.838 kb on - strand, within ymfAat 1180.854 kb on - strand, within ymfAat 1180.869 kb on - strand, within ymfAat 1180.881 kb on + strand, within ymfAat 1180.881 kb on + strand, within ymfAat 1180.890 kb on + strand, within ymfAat 1180.894 kb on + strand, within ymfAat 1180.894 kb on + strand, within ymfAat 1180.894 kb on + strand, within ymfAat 1180.900 kb on + strand, within ymfAat 1180.900 kb on + strand, within ymfAat 1180.902 kb on - strandat 1180.908 kb on - strandat 1180.937 kb on + strandat 1180.945 kb on - strandat 1180.945 kb on - strandat 1180.953 kb on - strandat 1180.959 kb on - strandat 1180.988 kb on - strandat 1180.988 kb on - strandat 1180.989 kb on - strandat 1180.989 kb on - strandat 1181.036 kb on - strandat 1181.046 kb on - strandat 1181.101 kb on + strandat 1181.101 kb on + strandat 1181.170 kb on - strand, within potDat 1181.280 kb on - strand, within potDat 1181.280 kb on - strand, within potDat 1181.421 kb on + strand, within potDat 1181.480 kb on - strand, within potDat 1181.522 kb on - strand, within potDat 1181.539 kb on + strand, within potDat 1181.547 kb on - strand, within potDat 1181.547 kb on - strand, within potDat 1181.556 kb on + strand, within potDat 1181.556 kb on + strand, within potDat 1181.581 kb on - strand, within potDat 1181.684 kb on - strand, within potDat 1181.721 kb on - strand, within potDat 1181.839 kb on - strand, within potDat 1181.865 kb on - strand, within potDat 1181.918 kb on + strand, within potDat 1182.003 kb on + strandat 1182.395 kb on + strand, within potCat 1182.474 kb on - strand, within potCat 1182.476 kb on - strand, within potCat 1182.524 kb on + strand, within potCat 1182.581 kb on + strand, within potCat 1182.581 kb on + strand, within potCat 1182.589 kb on + strand, within potCat 1182.668 kb on + strand, within potCat 1182.691 kb on + strand, within potCat 1182.700 kb on + strand, within potCat 1182.730 kb on + strand, within potCat 1182.910 kb on + strandat 1182.910 kb on + strandat 1182.914 kb on - strandat 1183.032 kb on + strand, within potBat 1183.032 kb on + strand, within potB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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1,180,234 - ycfZ b1121 0.67 +1.7
1,180,389 - ycfZ b1121 0.87 +1.7
1,180,389 - ycfZ b1121 0.87 +1.7
1,180,438 + +1.7
1,180,457 + +1.7
1,180,739 - ymfA b1122 0.55 -1.5
1,180,755 - ymfA b1122 0.58 -0.4
1,180,755 - ymfA b1122 0.58 +0.1
1,180,763 - ymfA b1122 0.60 +2.5
1,180,777 - ymfA b1122 0.63 +0.6
1,180,777 - ymfA b1122 0.63 +1.7
1,180,777 - ymfA b1122 0.63 -1.3
1,180,777 - ymfA b1122 0.63 +0.7
1,180,777 - ymfA b1122 0.63 +1.1
1,180,777 - ymfA b1122 0.63 -0.4
1,180,778 - ymfA b1122 0.63 -1.4
1,180,796 + ymfA b1122 0.67 +0.7
1,180,796 + ymfA b1122 0.67 +0.7
1,180,799 - ymfA b1122 0.68 -1.2
1,180,800 - ymfA b1122 0.68 +0.1
1,180,803 - ymfA b1122 0.68 +0.1
1,180,809 + ymfA b1122 0.70 +0.7
1,180,813 + ymfA b1122 0.71 -1.3
1,180,817 - ymfA b1122 0.71 +0.5
1,180,834 - ymfA b1122 0.75 +0.7
1,180,838 - ymfA b1122 0.76 +3.1
1,180,854 - ymfA b1122 0.79 -0.3
1,180,869 - ymfA b1122 0.83 -2.7
1,180,881 + ymfA b1122 0.85 +0.6
1,180,881 + ymfA b1122 0.85 -0.1
1,180,890 + ymfA b1122 0.87 -0.0
1,180,894 + ymfA b1122 0.88 -0.2
1,180,894 + ymfA b1122 0.88 +0.1
1,180,894 + ymfA b1122 0.88 -0.1
1,180,900 + ymfA b1122 0.89 -0.3
1,180,900 + ymfA b1122 0.89 +0.3
1,180,902 - -0.3
1,180,908 - -1.4
1,180,937 + -0.5
1,180,945 - +0.7
1,180,945 - -0.6
1,180,953 - -0.9
1,180,959 - -1.1
1,180,988 - +0.7
1,180,988 - +0.1
1,180,989 - -0.6
1,180,989 - -1.3
1,181,036 - -0.6
1,181,046 - -0.8
1,181,101 + +1.7
1,181,101 + +1.7
1,181,170 - potD b1123 0.16 -0.3
1,181,280 - potD b1123 0.26 -1.3
1,181,280 - potD b1123 0.26 -2.1
1,181,421 + potD b1123 0.40 -3.6
1,181,480 - potD b1123 0.45 -0.9
1,181,522 - potD b1123 0.49 -1.4
1,181,539 + potD b1123 0.51 -1.0
1,181,547 - potD b1123 0.52 -1.2
1,181,547 - potD b1123 0.52 -2.7
1,181,556 + potD b1123 0.53 +0.7
1,181,556 + potD b1123 0.53 -1.4
1,181,581 - potD b1123 0.55 -2.4
1,181,684 - potD b1123 0.65 -1.7
1,181,721 - potD b1123 0.68 -0.6
1,181,839 - potD b1123 0.80 +0.3
1,181,865 - potD b1123 0.82 -1.3
1,181,918 + potD b1123 0.87 -2.0
1,182,003 + -0.4
1,182,395 + potC b1124 0.44 -1.2
1,182,474 - potC b1124 0.53 -1.3
1,182,476 - potC b1124 0.54 -3.4
1,182,524 + potC b1124 0.60 -2.2
1,182,581 + potC b1124 0.67 -2.5
1,182,581 + potC b1124 0.67 -1.5
1,182,589 + potC b1124 0.68 -0.3
1,182,668 + potC b1124 0.78 -2.5
1,182,691 + potC b1124 0.81 +0.7
1,182,700 + potC b1124 0.82 -2.2
1,182,730 + potC b1124 0.86 -3.0
1,182,910 + -1.0
1,182,910 + -2.5
1,182,914 - -0.7
1,183,032 + potB b1125 0.23 -0.8
1,183,032 + potB b1125 0.23 -1.4

Or see this region's nucleotide sequence