Strain Fitness in Escherichia coli BW25113 around b1097

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfabF and pabC are separated by 119 nucleotidespabC and yceG are separated by 2 nucleotidesyceG and tmk overlap by 11 nucleotidestmk and holB overlap by 4 nucleotides b1095: fabF - 3-oxoacyl-(acyl carrier protein) synthase (NCBI), at 1,151,162 to 1,152,403 fabF b1096: pabC - 4-amino-4-deoxychorismate lyase (NCBI), at 1,152,523 to 1,153,332 pabC b1097: yceG - predicted aminodeoxychorismate lyase (NCBI), at 1,153,335 to 1,154,357 yceG b1098: tmk - thymidylate kinase (NCBI), at 1,154,347 to 1,154,988 tmk b1099: holB - DNA polymerase III subunit delta' (NCBI), at 1,154,985 to 1,155,989 holB Position (kb) 1153 1154 1155Strain fitness (log2 ratio) -1 0 1 2 3at 1152.335 kb on + strandat 1152.361 kb on - strandat 1152.361 kb on - strandat 1152.420 kb on + strandat 1152.420 kb on + strandat 1152.474 kb on - strandat 1152.482 kb on - strandat 1152.520 kb on + strandat 1152.520 kb on + strandat 1152.558 kb on + strandat 1152.676 kb on - strand, within pabCat 1152.788 kb on + strand, within pabCat 1152.788 kb on + strand, within pabCat 1152.876 kb on + strand, within pabCat 1152.902 kb on - strand, within pabCat 1152.909 kb on + strand, within pabCat 1152.909 kb on + strand, within pabCat 1153.012 kb on + strand, within pabCat 1153.171 kb on + strand, within pabCat 1153.177 kb on + strand, within pabCat 1153.208 kb on - strand, within pabCat 1153.223 kb on + strand, within pabCat 1153.301 kb on - strandat 1153.412 kb on + strandat 1153.412 kb on + strandat 1153.585 kb on - strand, within yceGat 1153.597 kb on + strand, within yceGat 1153.603 kb on - strand, within yceGat 1153.609 kb on + strand, within yceGat 1153.656 kb on + strand, within yceGat 1153.697 kb on - strand, within yceGat 1153.802 kb on + strand, within yceGat 1153.907 kb on + strand, within yceGat 1153.907 kb on + strand, within yceGat 1153.934 kb on + strand, within yceGat 1154.104 kb on - strand, within yceGat 1154.150 kb on + strand, within yceGat 1154.150 kb on + strand, within yceGat 1154.152 kb on - strand, within yceGat 1154.152 kb on - strand, within yceGat 1154.158 kb on - strand, within yceGat 1154.158 kb on - strand, within yceGat 1154.160 kb on - strand, within yceGat 1154.280 kb on - strandat 1154.323 kb on + strandat 1154.323 kb on + strandat 1155.056 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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1,152,335 + +2.0
1,152,361 - +3.1
1,152,361 - +1.5
1,152,420 + +1.1
1,152,420 + +1.4
1,152,474 - -0.6
1,152,482 - -0.1
1,152,520 + +1.7
1,152,520 + +1.7
1,152,558 + +1.1
1,152,676 - pabC b1096 0.19 +0.1
1,152,788 + pabC b1096 0.33 +1.2
1,152,788 + pabC b1096 0.33 +0.1
1,152,876 + pabC b1096 0.44 -1.3
1,152,902 - pabC b1096 0.47 -0.9
1,152,909 + pabC b1096 0.48 +0.2
1,152,909 + pabC b1096 0.48 +2.0
1,153,012 + pabC b1096 0.60 +0.8
1,153,171 + pabC b1096 0.80 +1.6
1,153,177 + pabC b1096 0.81 +2.5
1,153,208 - pabC b1096 0.85 +0.5
1,153,223 + pabC b1096 0.86 +3.1
1,153,301 - -0.9
1,153,412 + +2.7
1,153,412 + +2.6
1,153,585 - yceG b1097 0.24 +0.1
1,153,597 + yceG b1097 0.26 +3.7
1,153,603 - yceG b1097 0.26 +2.2
1,153,609 + yceG b1097 0.27 +2.2
1,153,656 + yceG b1097 0.31 +2.4
1,153,697 - yceG b1097 0.35 +3.2
1,153,802 + yceG b1097 0.46 +2.3
1,153,907 + yceG b1097 0.56 +2.6
1,153,907 + yceG b1097 0.56 +2.4
1,153,934 + yceG b1097 0.59 +3.0
1,154,104 - yceG b1097 0.75 +2.7
1,154,150 + yceG b1097 0.80 +3.1
1,154,150 + yceG b1097 0.80 +3.0
1,154,152 - yceG b1097 0.80 +1.7
1,154,152 - yceG b1097 0.80 +3.2
1,154,158 - yceG b1097 0.80 +2.7
1,154,158 - yceG b1097 0.80 +3.4
1,154,160 - yceG b1097 0.81 +1.2
1,154,280 - +2.4
1,154,323 + +1.1
1,154,323 + +0.1
1,155,056 + -1.3

Or see this region's nucleotide sequence