Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafT and ykfM are separated by 136 nucleotides ykfM and yafU are separated by 9 nucleotides yafU and yafF are separated by 3 nucleotides yafF and yafV are separated by 40 nucleotides
b0217: yafT - predicted aminopeptidase (NCBI), at 237,335 to 238,120
yafT
b4586: ykfM - hypothetical protein, no homologs (RefSeq), at 238,257 to 238,736
ykfM
b0218: yafU - predicted inner membrane protein (RefSeq), at 238,746 to 239,102
yafU
b4503: yafF - no description, at 239,106 to 239,378
yafF
b0219: yafV - predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI), at 239,419 to 240,189
yafV
Position (kb) 238
239
240 Strain fitness (log2 ratio) -1
0
1
2
3 at 237.775 kb on + strand, within yafT at 237.908 kb on - strand, within yafT at 237.911 kb on - strand, within yafT at 237.913 kb on + strand, within yafT at 237.937 kb on + strand, within yafT at 237.937 kb on + strand, within yafT at 237.943 kb on + strand, within yafT at 237.951 kb on + strand, within yafT at 237.953 kb on + strand, within yafT at 237.953 kb on + strand, within yafT at 237.963 kb on + strand, within yafT at 238.027 kb on + strand, within yafT at 238.027 kb on + strand, within yafT at 238.029 kb on + strand, within yafT at 238.036 kb on + strand, within yafT at 238.037 kb on + strand, within yafT at 238.037 kb on + strand, within yafT at 238.037 kb on + strand, within yafT at 238.037 kb on + strand, within yafT at 238.038 kb on + strand, within yafT at 238.046 kb on + strand at 238.046 kb on + strand at 238.063 kb on + strand at 238.063 kb on + strand at 238.734 kb on - strand at 238.901 kb on + strand, within yafU at 238.910 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 238.915 kb on - strand, within yafU at 239.013 kb on + strand, within yafU at 239.013 kb on + strand, within yafU at 239.013 kb on + strand, within yafU at 239.036 kb on - strand, within yafU at 239.036 kb on - strand, within yafU at 239.163 kb on + strand, within yafF at 239.166 kb on + strand, within yafF at 239.392 kb on - strand at 239.412 kb on + strand at 239.414 kb on - strand at 239.417 kb on + strand at 239.426 kb on + strand at 239.436 kb on - strand at 239.436 kb on - strand at 239.850 kb on - strand, within yafV at 239.850 kb on - strand, within yafV at 239.907 kb on + strand, within yafV at 240.066 kb on + strand, within yafV at 240.072 kb on + strand, within yafV at 240.098 kb on - strand, within yafV
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM remove 237,775 + yafT b0217 0.56 +1.9 237,908 - yafT b0217 0.73 +0.9 237,911 - yafT b0217 0.73 +1.9 237,913 + yafT b0217 0.74 +0.9 237,937 + yafT b0217 0.77 -0.7 237,937 + yafT b0217 0.77 +0.9 237,943 + yafT b0217 0.77 +0.9 237,951 + yafT b0217 0.78 +1.3 237,953 + yafT b0217 0.79 +0.3 237,953 + yafT b0217 0.79 -0.7 237,963 + yafT b0217 0.80 +0.2 238,027 + yafT b0217 0.88 +3.5 238,027 + yafT b0217 0.88 +0.6 238,029 + yafT b0217 0.88 -0.4 238,036 + yafT b0217 0.89 +0.2 238,037 + yafT b0217 0.89 +1.5 238,037 + yafT b0217 0.89 +1.4 238,037 + yafT b0217 0.89 +0.6 238,037 + yafT b0217 0.89 +1.1 238,038 + yafT b0217 0.89 +1.2 238,046 + +1.3 238,046 + +0.5 238,063 + -0.1 238,063 + +0.2 238,734 - +1.9 238,901 + yafU b0218 0.43 +1.9 238,910 - yafU b0218 0.46 -0.4 238,915 - yafU b0218 0.47 +0.9 238,915 - yafU b0218 0.47 -0.4 238,915 - yafU b0218 0.47 -1.3 239,013 + yafU b0218 0.75 +1.9 239,013 + yafU b0218 0.75 +1.9 239,013 + yafU b0218 0.75 +1.9 239,036 - yafU b0218 0.81 -0.6 239,036 - yafU b0218 0.81 -1.7 239,163 + yafF b4503 0.21 +0.9 239,166 + yafF b4503 0.22 -0.4 239,392 - -0.0 239,412 + -0.9 239,414 - +0.2 239,417 + -1.6 239,426 + -0.3 239,436 - +0.8 239,436 - -0.2 239,850 - yafV b0219 0.56 +1.1 239,850 - yafV b0219 0.56 -1.6 239,907 + yafV b0219 0.63 +0.7 240,066 + yafV b0219 0.84 +0.4 240,072 + yafV b0219 0.85 +0.9 240,098 - yafV b0219 0.88 +1.3
Or see this region's nucleotide sequence