Strain Fitness in Escherichia coli BW25113 around b0018

Experiment: LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntinsL-1 and mokC are separated by 193 nucleotidesmokC and hokC overlap by 210 nucleotideshokC and sokC are separated by 48 nucleotidessokC and nhaA are separated by 482 nucleotides b0016: insL-1 - IS186/IS421 transposase (NCBI), at 15,445 to 16,557 insL-1 b0018: mokC - regulatory protein for HokC, overlaps CDS of hokC (NCBI), at 16,751 to 16,960 mokC b4412: hokC - toxic membrane protein, small (NCBI), at 16,751 to 16,903 hokC b4413: sokC - regulatory antisense RNA (NCBI), at 16,952 to 17,006 sokC b0019: nhaA - pH-dependent sodium/proton antiporter (NCBI), at 17,489 to 18,655 nhaA Position (kb) 16 17Strain fitness (log2 ratio) -2 -1 0 1 2at 16.703 kb on + strandat 16.729 kb on + strandat 16.729 kb on + strandat 16.895 kb on - strandat 16.895 kb on - strandat 16.920 kb on + strand, within mokCat 16.942 kb on + strandat 16.950 kb on - strandat 16.950 kb on - strandat 16.950 kb on - strandat 16.950 kb on - strandat 16.950 kb on - strandat 16.950 kb on - strandat 16.955 kb on - strandat 16.957 kb on + strandat 16.957 kb on + strandat 16.957 kb on + strandat 16.976 kb on - strand, within sokCat 17.024 kb on - strandat 17.032 kb on + strandat 17.037 kb on + strandat 17.037 kb on + strandat 17.058 kb on + strandat 17.058 kb on + strandat 17.058 kb on + strandat 17.060 kb on + strandat 17.060 kb on + strandat 17.096 kb on + strandat 17.097 kb on + strandat 17.104 kb on + strandat 17.104 kb on + strandat 17.112 kb on - strandat 17.306 kb on + strandat 17.318 kb on - strandat 17.330 kb on - strandat 17.339 kb on - strandat 17.339 kb on - strandat 17.503 kb on - strandat 17.517 kb on - strandat 17.517 kb on - strandat 17.517 kb on - strandat 17.537 kb on + strandat 17.555 kb on + strandat 17.581 kb on - strandat 17.600 kb on + strandat 17.621 kb on + strand, within nhaAat 17.671 kb on + strand, within nhaAat 17.676 kb on - strandat 17.749 kb on - strand, within nhaAat 17.749 kb on - strand, within nhaAat 17.765 kb on - strand, within nhaAat 17.765 kb on - strand, within nhaAat 17.813 kb on + strand, within nhaAat 17.879 kb on + strand, within nhaAat 17.887 kb on - strand, within nhaAat 17.902 kb on - strand, within nhaAat 17.904 kb on + strand, within nhaAat 17.917 kb on + strand, within nhaAat 17.925 kb on - strand, within nhaAat 17.925 kb on - strand, within nhaAat 17.925 kb on - strand, within nhaA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Vancomycin Hydrochloride from Streptomyces orientalis 0.00003125 mM
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16,703 + +0.3
16,729 + -0.5
16,729 + +1.1
16,895 - -1.4
16,895 - -0.3
16,920 + mokC b0018 0.80 -1.6
16,942 + -0.5
16,950 - -0.9
16,950 - -0.5
16,950 - +0.3
16,950 - -0.3
16,950 - +0.2
16,950 - -0.5
16,955 - -0.9
16,957 + +0.6
16,957 + -1.3
16,957 + -0.2
16,976 - sokC b4413 0.44 -1.1
17,024 - +0.6
17,032 + -0.8
17,037 + -1.1
17,037 + +1.6
17,058 + -0.2
17,058 + -0.4
17,058 + -0.2
17,060 + -1.4
17,060 + -2.0
17,096 + -1.1
17,097 + -0.1
17,104 + +0.9
17,104 + -0.7
17,112 - -0.6
17,306 + +1.9
17,318 - -1.1
17,330 - -0.3
17,339 - -1.2
17,339 - -0.4
17,503 - +0.3
17,517 - +0.0
17,517 - +0.9
17,517 - -0.3
17,537 + +0.4
17,555 + +0.5
17,581 - +0.0
17,600 + -2.3
17,621 + nhaA b0019 0.11 +0.1
17,671 + nhaA b0019 0.16 +0.8
17,676 - +0.3
17,749 - nhaA b0019 0.22 -0.3
17,749 - nhaA b0019 0.22 +1.7
17,765 - nhaA b0019 0.24 -1.0
17,765 - nhaA b0019 0.24 -1.3
17,813 + nhaA b0019 0.28 +0.6
17,879 + nhaA b0019 0.33 +0.2
17,887 - nhaA b0019 0.34 -0.7
17,902 - nhaA b0019 0.35 +0.8
17,904 + nhaA b0019 0.36 +0.3
17,917 + nhaA b0019 0.37 -1.2
17,925 - nhaA b0019 0.37 +1.2
17,925 - nhaA b0019 0.37 -1.4
17,925 - nhaA b0019 0.37 +1.1

Or see this region's nucleotide sequence