Experiment: soil sample 5; outgrowth in LB
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PS417_06265 and PS417_06270 overlap by 4 nucleotides PS417_06270 and PS417_06275 are separated by 2 nucleotides PS417_06275 and PS417_06280 overlap by 4 nucleotides PS417_06280 and PS417_06285 are separated by 73 nucleotides
PS417_06265: PS417_06265 - 3-hydroxyacyl-ACP dehydratase, at 1,390,435 to 1,390,875
_06265
PS417_06270: PS417_06270 - UDP-N-acetylglucosamine acyltransferase, at 1,390,872 to 1,391,648
_06270
PS417_06275: PS417_06275 - lipid-A-disaccharide synthase, at 1,391,651 to 1,392,790
_06275
PS417_06280: PS417_06280 - ribonuclease HII, at 1,392,787 to 1,393,419
_06280
PS417_06285: PS417_06285 - DNA polymerase III subunit alpha, at 1,393,493 to 1,397,014
_06285
Position (kb) 1391
1392
1393 Strain fitness (log2 ratio) -1
0
1
2
3 at 1392.979 kb on + strand at 1393.268 kb on - strand, within PS417_06280 at 1393.268 kb on - strand, within PS417_06280 at 1393.418 kb on - strand at 1393.418 kb on - strand at 1393.479 kb on + strand at 1393.479 kb on + strand at 1393.479 kb on + strand at 1393.479 kb on + strand at 1393.479 kb on + strand at 1393.481 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction soil sample 5; outgrowth in LB remove 1,392,979 + +0.5 1,393,268 - PS417_06280 0.76 -0.6 1,393,268 - PS417_06280 0.76 -0.7 1,393,418 - +0.1 1,393,418 - -1.1 1,393,479 + +2.8 1,393,479 + -0.6 1,393,479 + -1.3 1,393,479 + -0.1 1,393,479 + -0.2 1,393,481 + -0.3
Or see this region's nucleotide sequence