Experiment: LB Anaerobic with Sulfamonomethoxine 0.0000625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yrfA and yrfB overlap by 11 nucleotides yrfB and yrfC overlap by 17 nucleotides yrfC and yrfD overlap by 1 nucleotides yrfD and mrcA are separated by 119 nucleotides
b3392: yrfA - orf, hypothetical protein (VIMSS), at 3,518,637 to 3,519,041
yrfA
b3393: yrfB - hypothetical protein (NCBI), at 3,519,031 to 3,519,471
yrfB
b3394: yrfC - predicted fimbrial assembly protein (NCBI), at 3,519,455 to 3,519,994
yrfC
b3395: yrfD - orf, hypothetical protein (VIMSS), at 3,519,994 to 3,520,773
yrfD
b3396: mrcA - peptidoglycan synthetase; penicillin-binding protein 1A (VIMSS), at 3,520,893 to 3,523,445
mrcA
Position (kb)
3519
3520
3521 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3519.000 kb on + strand, within yrfA at 3519.013 kb on - strand at 3519.013 kb on - strand at 3519.013 kb on - strand at 3519.027 kb on + strand at 3519.027 kb on + strand at 3519.027 kb on + strand at 3519.113 kb on + strand, within yrfB at 3519.123 kb on + strand, within yrfB at 3519.123 kb on + strand, within yrfB at 3519.150 kb on + strand, within yrfB at 3519.189 kb on + strand, within yrfB at 3519.189 kb on + strand, within yrfB at 3519.290 kb on + strand, within yrfB at 3519.292 kb on + strand, within yrfB at 3519.363 kb on - strand, within yrfB at 3519.429 kb on + strand at 3519.471 kb on + strand at 3519.471 kb on + strand at 3519.483 kb on + strand at 3519.509 kb on + strand, within yrfC at 3519.509 kb on + strand, within yrfC at 3519.529 kb on + strand, within yrfC at 3519.556 kb on - strand, within yrfC at 3519.556 kb on - strand, within yrfC at 3519.608 kb on - strand, within yrfC at 3519.609 kb on + strand, within yrfC at 3519.609 kb on + strand, within yrfC at 3519.641 kb on - strand, within yrfC at 3519.731 kb on - strand, within yrfC at 3519.877 kb on + strand, within yrfC at 3519.885 kb on - strand, within yrfC at 3519.971 kb on - strand at 3519.995 kb on + strand at 3520.003 kb on - strand at 3520.079 kb on + strand, within yrfD at 3520.098 kb on - strand, within yrfD at 3520.153 kb on - strand, within yrfD at 3520.163 kb on - strand, within yrfD at 3520.193 kb on + strand, within yrfD at 3520.193 kb on + strand, within yrfD at 3520.201 kb on - strand, within yrfD at 3520.215 kb on + strand, within yrfD at 3520.215 kb on + strand, within yrfD at 3520.276 kb on + strand, within yrfD at 3520.279 kb on + strand, within yrfD at 3520.289 kb on - strand, within yrfD at 3520.357 kb on - strand, within yrfD at 3520.357 kb on - strand, within yrfD at 3520.388 kb on - strand, within yrfD at 3520.409 kb on - strand, within yrfD at 3520.427 kb on + strand, within yrfD at 3520.434 kb on + strand at 3520.436 kb on + strand, within yrfD at 3520.436 kb on + strand, within yrfD at 3520.437 kb on - strand, within yrfD at 3520.468 kb on - strand, within yrfD at 3520.586 kb on + strand, within yrfD at 3520.637 kb on + strand, within yrfD at 3520.637 kb on + strand, within yrfD at 3520.775 kb on + strand at 3520.793 kb on - strand at 3520.831 kb on + strand at 3520.918 kb on + strand at 3520.928 kb on + strand at 3520.928 kb on - strand at 3520.928 kb on - strand at 3520.938 kb on - strand at 3520.962 kb on + strand at 3520.970 kb on + strand at 3520.970 kb on + strand at 3520.970 kb on + strand at 3520.970 kb on - strand at 3520.978 kb on - strand at 3520.994 kb on - strand at 3521.004 kb on - strand at 3521.004 kb on - strand at 3521.047 kb on - strand at 3521.047 kb on - strand at 3521.052 kb on + strand at 3521.067 kb on + strand at 3521.075 kb on - strand at 3521.075 kb on - strand at 3521.085 kb on - strand at 3521.085 kb on - strand at 3521.184 kb on - strand, within mrcA at 3521.198 kb on - strand, within mrcA at 3521.238 kb on + strand, within mrcA at 3521.251 kb on + strand, within mrcA at 3521.251 kb on - strand, within mrcA at 3521.256 kb on + strand, within mrcA at 3521.256 kb on + strand, within mrcA at 3521.282 kb on + strand, within mrcA at 3521.287 kb on + strand, within mrcA at 3521.295 kb on - strand, within mrcA at 3521.295 kb on - strand, within mrcA at 3521.343 kb on + strand, within mrcA at 3521.349 kb on - strand, within mrcA at 3521.361 kb on + strand, within mrcA at 3521.366 kb on - strand, within mrcA at 3521.366 kb on - strand, within mrcA at 3521.408 kb on + strand, within mrcA at 3521.408 kb on + strand at 3521.408 kb on + strand, within mrcA at 3521.408 kb on + strand, within mrcA at 3521.416 kb on - strand, within mrcA at 3521.443 kb on - strand, within mrcA at 3521.525 kb on + strand, within mrcA at 3521.555 kb on + strand, within mrcA at 3521.555 kb on + strand, within mrcA at 3521.555 kb on + strand, within mrcA at 3521.555 kb on + strand, within mrcA at 3521.555 kb on + strand, within mrcA at 3521.561 kb on - strand, within mrcA at 3521.591 kb on - strand, within mrcA at 3521.591 kb on - strand, within mrcA at 3521.599 kb on + strand, within mrcA at 3521.599 kb on + strand, within mrcA at 3521.599 kb on - strand, within mrcA at 3521.601 kb on + strand, within mrcA at 3521.601 kb on + strand, within mrcA at 3521.607 kb on - strand, within mrcA at 3521.626 kb on - strand, within mrcA at 3521.626 kb on - strand, within mrcA at 3521.626 kb on - strand, within mrcA at 3521.694 kb on - strand, within mrcA at 3521.694 kb on - strand, within mrcA at 3521.694 kb on - strand, within mrcA at 3521.694 kb on - strand, within mrcA at 3521.708 kb on + strand, within mrcA at 3521.728 kb on + strand, within mrcA at 3521.728 kb on + strand, within mrcA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Sulfamonomethoxine 0.0000625 mM remove 3,519,000 + yrfA b3392 0.90 -0.7 3,519,013 - -0.3 3,519,013 - +0.1 3,519,013 - -0.8 3,519,027 + -0.1 3,519,027 + -0.2 3,519,027 + +0.3 3,519,113 + yrfB b3393 0.19 +0.3 3,519,123 + yrfB b3393 0.21 -0.4 3,519,123 + yrfB b3393 0.21 -0.2 3,519,150 + yrfB b3393 0.27 +0.0 3,519,189 + yrfB b3393 0.36 -0.6 3,519,189 + yrfB b3393 0.36 +0.4 3,519,290 + yrfB b3393 0.59 -1.1 3,519,292 + yrfB b3393 0.59 -1.2 3,519,363 - yrfB b3393 0.75 -0.1 3,519,429 + +0.2 3,519,471 + -0.3 3,519,471 + +0.3 3,519,483 + -2.1 3,519,509 + yrfC b3394 0.10 -0.3 3,519,509 + yrfC b3394 0.10 -0.3 3,519,529 + yrfC b3394 0.14 -0.4 3,519,556 - yrfC b3394 0.19 -0.6 3,519,556 - yrfC b3394 0.19 -0.5 3,519,608 - yrfC b3394 0.28 +0.0 3,519,609 + yrfC b3394 0.29 -1.7 3,519,609 + yrfC b3394 0.29 -0.5 3,519,641 - yrfC b3394 0.34 -0.8 3,519,731 - yrfC b3394 0.51 -0.9 3,519,877 + yrfC b3394 0.78 -0.4 3,519,885 - yrfC b3394 0.80 +0.5 3,519,971 - -0.3 3,519,995 + -1.6 3,520,003 - -0.3 3,520,079 + yrfD b3395 0.11 -2.0 3,520,098 - yrfD b3395 0.13 -1.2 3,520,153 - yrfD b3395 0.20 -0.1 3,520,163 - yrfD b3395 0.22 +0.4 3,520,193 + yrfD b3395 0.26 -1.8 3,520,193 + yrfD b3395 0.26 -1.1 3,520,201 - yrfD b3395 0.27 -0.2 3,520,215 + yrfD b3395 0.28 -0.4 3,520,215 + yrfD b3395 0.28 -1.7 3,520,276 + yrfD b3395 0.36 -0.7 3,520,279 + yrfD b3395 0.37 -0.8 3,520,289 - yrfD b3395 0.38 -0.5 3,520,357 - yrfD b3395 0.47 -0.0 3,520,357 - yrfD b3395 0.47 -0.6 3,520,388 - yrfD b3395 0.51 +0.7 3,520,409 - yrfD b3395 0.53 +0.1 3,520,427 + yrfD b3395 0.56 -1.2 3,520,434 + -0.3 3,520,436 + yrfD b3395 0.57 -2.0 3,520,436 + yrfD b3395 0.57 -1.1 3,520,437 - yrfD b3395 0.57 -1.0 3,520,468 - yrfD b3395 0.61 -0.8 3,520,586 + yrfD b3395 0.76 +0.6 3,520,637 + yrfD b3395 0.82 +0.3 3,520,637 + yrfD b3395 0.82 -1.5 3,520,775 + +0.1 3,520,793 - +0.7 3,520,831 + -0.8 3,520,918 + -0.7 3,520,928 + +0.5 3,520,928 - -2.5 3,520,928 - +0.4 3,520,938 - +0.0 3,520,962 + -0.9 3,520,970 + -0.2 3,520,970 + -0.6 3,520,970 + +0.4 3,520,970 - -0.7 3,520,978 - -0.2 3,520,994 - +0.1 3,521,004 - +0.4 3,521,004 - -0.9 3,521,047 - -1.1 3,521,047 - +0.9 3,521,052 + -1.9 3,521,067 + -1.1 3,521,075 - -2.1 3,521,075 - -0.3 3,521,085 - +0.1 3,521,085 - -0.5 3,521,184 - mrcA b3396 0.11 +0.4 3,521,198 - mrcA b3396 0.12 -1.5 3,521,238 + mrcA b3396 0.14 +0.3 3,521,251 + mrcA b3396 0.14 -0.5 3,521,251 - mrcA b3396 0.14 -1.3 3,521,256 + mrcA b3396 0.14 +0.8 3,521,256 + mrcA b3396 0.14 +0.4 3,521,282 + mrcA b3396 0.15 -0.7 3,521,287 + mrcA b3396 0.15 -0.0 3,521,295 - mrcA b3396 0.16 -2.3 3,521,295 - mrcA b3396 0.16 +1.3 3,521,343 + mrcA b3396 0.18 -1.5 3,521,349 - mrcA b3396 0.18 +0.2 3,521,361 + mrcA b3396 0.18 -0.1 3,521,366 - mrcA b3396 0.19 -1.2 3,521,366 - mrcA b3396 0.19 -0.6 3,521,408 + mrcA b3396 0.20 -2.0 3,521,408 + -1.9 3,521,408 + mrcA b3396 0.20 -1.0 3,521,408 + mrcA b3396 0.20 -1.4 3,521,416 - mrcA b3396 0.20 -1.2 3,521,443 - mrcA b3396 0.22 +0.3 3,521,525 + mrcA b3396 0.25 -1.4 3,521,555 + mrcA b3396 0.26 +0.0 3,521,555 + mrcA b3396 0.26 -1.2 3,521,555 + mrcA b3396 0.26 +0.1 3,521,555 + mrcA b3396 0.26 -0.2 3,521,555 + mrcA b3396 0.26 -0.9 3,521,561 - mrcA b3396 0.26 +1.0 3,521,591 - mrcA b3396 0.27 +0.7 3,521,591 - mrcA b3396 0.27 +1.5 3,521,599 + mrcA b3396 0.28 -0.3 3,521,599 + mrcA b3396 0.28 -0.1 3,521,599 - mrcA b3396 0.28 -0.2 3,521,601 + mrcA b3396 0.28 +0.1 3,521,601 + mrcA b3396 0.28 -1.7 3,521,607 - mrcA b3396 0.28 -0.4 3,521,626 - mrcA b3396 0.29 -0.3 3,521,626 - mrcA b3396 0.29 -1.1 3,521,626 - mrcA b3396 0.29 -0.1 3,521,694 - mrcA b3396 0.31 -0.2 3,521,694 - mrcA b3396 0.31 +3.2 3,521,694 - mrcA b3396 0.31 -0.5 3,521,694 - mrcA b3396 0.31 +0.8 3,521,708 + mrcA b3396 0.32 -2.7 3,521,728 + mrcA b3396 0.33 -0.8 3,521,728 + mrcA b3396 0.33 -2.0
Or see this region's nucleotide sequence