Strain Fitness in Escherichia coli BW25113 around b2994

Experiment: LB Anaerobic with Sulfamonomethoxine 0.0000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthybF and hybE overlap by 8 nucleotideshybE and hybD overlap by 8 nucleotideshybD and hybC overlap by 1 nucleotideshybC and hybB overlap by 4 nucleotides b2991: hybF - protein involved with the maturation of hydrogenases 1 and 2 (NCBI), at 3,137,999 to 3,138,340 hybF b2992: hybE - hydrogenase 2-specific chaperone (NCBI), at 3,138,333 to 3,138,821 hybE b2993: hybD - predicted maturation element for hydrogenase 2 (NCBI), at 3,138,814 to 3,139,308 hybD b2994: hybC - hydrogenase 2, large subunit (NCBI), at 3,139,308 to 3,141,011 hybC b2995: hybB - predicted hydrogenase 2 cytochrome b type component (NCBI), at 3,141,008 to 3,142,186 hybB Position (kb) 3139 3140 3141 3142Strain fitness (log2 ratio) -2 -1 0 1at 3138.363 kb on + strandat 3138.474 kb on + strand, within hybEat 3138.474 kb on + strand, within hybEat 3138.640 kb on + strand, within hybEat 3138.640 kb on + strand, within hybEat 3138.649 kb on + strand, within hybEat 3138.658 kb on - strand, within hybEat 3138.707 kb on + strand, within hybEat 3138.715 kb on - strand, within hybEat 3138.839 kb on - strandat 3138.839 kb on - strandat 3138.928 kb on + strand, within hybDat 3139.075 kb on - strand, within hybDat 3139.075 kb on - strand, within hybDat 3139.157 kb on + strand, within hybDat 3139.227 kb on + strand, within hybDat 3139.242 kb on - strand, within hybDat 3139.245 kb on + strand, within hybDat 3139.288 kb on + strandat 3139.308 kb on + strandat 3139.371 kb on - strandat 3139.371 kb on - strandat 3139.379 kb on + strandat 3139.379 kb on + strandat 3139.379 kb on + strandat 3139.396 kb on + strandat 3139.443 kb on - strandat 3139.538 kb on - strand, within hybCat 3139.613 kb on - strand, within hybCat 3139.676 kb on + strand, within hybCat 3139.683 kb on + strand, within hybCat 3139.701 kb on + strand, within hybCat 3139.716 kb on + strand, within hybCat 3139.763 kb on + strand, within hybCat 3139.832 kb on - strand, within hybCat 3139.864 kb on + strand, within hybCat 3140.001 kb on - strand, within hybCat 3140.013 kb on - strand, within hybCat 3140.013 kb on - strand, within hybCat 3140.129 kb on + strand, within hybCat 3140.159 kb on + strand, within hybCat 3140.167 kb on - strand, within hybCat 3140.167 kb on - strand, within hybCat 3140.227 kb on + strandat 3140.227 kb on + strand, within hybCat 3140.235 kb on + strand, within hybCat 3140.284 kb on + strand, within hybCat 3140.320 kb on - strand, within hybCat 3140.361 kb on + strand, within hybCat 3140.391 kb on - strand, within hybCat 3140.418 kb on + strand, within hybCat 3140.418 kb on + strand, within hybCat 3140.525 kb on - strand, within hybCat 3140.583 kb on + strand, within hybCat 3140.583 kb on + strand, within hybCat 3140.583 kb on + strand, within hybCat 3140.583 kb on + strand, within hybCat 3140.591 kb on - strand, within hybCat 3140.591 kb on - strand, within hybCat 3140.641 kb on - strand, within hybCat 3140.657 kb on + strand, within hybCat 3140.657 kb on + strand, within hybCat 3140.823 kb on - strand, within hybCat 3140.838 kb on + strand, within hybCat 3140.838 kb on + strand, within hybCat 3140.852 kb on - strandat 3140.885 kb on + strandat 3141.123 kb on + strandat 3141.192 kb on - strand, within hybBat 3141.215 kb on + strand, within hybBat 3141.215 kb on + strand, within hybBat 3141.223 kb on - strand, within hybBat 3141.240 kb on - strand, within hybBat 3141.240 kb on - strand, within hybBat 3141.286 kb on - strand, within hybBat 3141.341 kb on - strand, within hybBat 3141.341 kb on - strand, within hybBat 3141.472 kb on + strand, within hybBat 3141.486 kb on + strand, within hybBat 3141.554 kb on - strand, within hybBat 3141.632 kb on + strand, within hybBat 3141.642 kb on + strand, within hybBat 3141.642 kb on + strand, within hybBat 3141.657 kb on - strand, within hybBat 3141.676 kb on - strand, within hybBat 3141.733 kb on - strand, within hybBat 3141.738 kb on - strand, within hybBat 3141.738 kb on - strand, within hybBat 3141.751 kb on - strand, within hybBat 3141.751 kb on - strand, within hybBat 3141.779 kb on - strand, within hybBat 3141.804 kb on - strand, within hybBat 3141.808 kb on + strand, within hybBat 3141.843 kb on + strand, within hybBat 3141.851 kb on - strand, within hybBat 3141.888 kb on + strand, within hybBat 3141.901 kb on - strand, within hybBat 3141.901 kb on - strand, within hybBat 3141.913 kb on - strand, within hybBat 3141.955 kb on - strand, within hybBat 3141.955 kb on - strand, within hybB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Sulfamonomethoxine 0.0000625 mM
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3,138,363 + +0.3
3,138,474 + hybE b2992 0.29 -0.2
3,138,474 + hybE b2992 0.29 -0.5
3,138,640 + hybE b2992 0.63 +0.2
3,138,640 + hybE b2992 0.63 -0.5
3,138,649 + hybE b2992 0.65 -0.7
3,138,658 - hybE b2992 0.66 -1.4
3,138,707 + hybE b2992 0.76 -0.8
3,138,715 - hybE b2992 0.78 -0.4
3,138,839 - +0.1
3,138,839 - +0.4
3,138,928 + hybD b2993 0.23 -0.4
3,139,075 - hybD b2993 0.53 -2.6
3,139,075 - hybD b2993 0.53 -0.7
3,139,157 + hybD b2993 0.69 -1.6
3,139,227 + hybD b2993 0.83 -1.2
3,139,242 - hybD b2993 0.86 -1.3
3,139,245 + hybD b2993 0.87 -0.9
3,139,288 + -1.5
3,139,308 + +0.1
3,139,371 - -0.7
3,139,371 - -1.2
3,139,379 + -0.6
3,139,379 + -1.0
3,139,379 + +0.1
3,139,396 + -1.0
3,139,443 - -2.5
3,139,538 - hybC b2994 0.13 -1.2
3,139,613 - hybC b2994 0.18 -2.2
3,139,676 + hybC b2994 0.22 -0.4
3,139,683 + hybC b2994 0.22 -0.9
3,139,701 + hybC b2994 0.23 -2.1
3,139,716 + hybC b2994 0.24 -0.4
3,139,763 + hybC b2994 0.27 -1.0
3,139,832 - hybC b2994 0.31 -1.3
3,139,864 + hybC b2994 0.33 -0.4
3,140,001 - hybC b2994 0.41 -1.6
3,140,013 - hybC b2994 0.41 -1.3
3,140,013 - hybC b2994 0.41 -1.1
3,140,129 + hybC b2994 0.48 -1.4
3,140,159 + hybC b2994 0.50 -0.4
3,140,167 - hybC b2994 0.50 -0.8
3,140,167 - hybC b2994 0.50 -0.9
3,140,227 + -0.0
3,140,227 + hybC b2994 0.54 -0.2
3,140,235 + hybC b2994 0.54 -0.8
3,140,284 + hybC b2994 0.57 -0.4
3,140,320 - hybC b2994 0.59 -0.9
3,140,361 + hybC b2994 0.62 -0.8
3,140,391 - hybC b2994 0.64 -1.3
3,140,418 + hybC b2994 0.65 -2.5
3,140,418 + hybC b2994 0.65 -0.8
3,140,525 - hybC b2994 0.71 -1.2
3,140,583 + hybC b2994 0.75 -1.0
3,140,583 + hybC b2994 0.75 -1.2
3,140,583 + hybC b2994 0.75 -0.9
3,140,583 + hybC b2994 0.75 -1.7
3,140,591 - hybC b2994 0.75 -0.7
3,140,591 - hybC b2994 0.75 -0.6
3,140,641 - hybC b2994 0.78 -1.7
3,140,657 + hybC b2994 0.79 -1.0
3,140,657 + hybC b2994 0.79 -0.6
3,140,823 - hybC b2994 0.89 -1.5
3,140,838 + hybC b2994 0.90 +0.1
3,140,838 + hybC b2994 0.90 -1.0
3,140,852 - +0.5
3,140,885 + +0.2
3,141,123 + +0.2
3,141,192 - hybB b2995 0.16 +0.1
3,141,215 + hybB b2995 0.18 -0.6
3,141,215 + hybB b2995 0.18 -0.7
3,141,223 - hybB b2995 0.18 -0.9
3,141,240 - hybB b2995 0.20 -2.7
3,141,240 - hybB b2995 0.20 -0.5
3,141,286 - hybB b2995 0.24 -1.0
3,141,341 - hybB b2995 0.28 +0.6
3,141,341 - hybB b2995 0.28 -2.0
3,141,472 + hybB b2995 0.39 -0.3
3,141,486 + hybB b2995 0.41 +0.3
3,141,554 - hybB b2995 0.46 -0.6
3,141,632 + hybB b2995 0.53 +0.3
3,141,642 + hybB b2995 0.54 -1.1
3,141,642 + hybB b2995 0.54 -0.7
3,141,657 - hybB b2995 0.55 -0.2
3,141,676 - hybB b2995 0.57 -0.1
3,141,733 - hybB b2995 0.61 +0.5
3,141,738 - hybB b2995 0.62 -0.4
3,141,738 - hybB b2995 0.62 -1.2
3,141,751 - hybB b2995 0.63 -0.1
3,141,751 - hybB b2995 0.63 -0.1
3,141,779 - hybB b2995 0.65 -0.4
3,141,804 - hybB b2995 0.68 -0.2
3,141,808 + hybB b2995 0.68 -0.8
3,141,843 + hybB b2995 0.71 -1.6
3,141,851 - hybB b2995 0.72 -1.1
3,141,888 + hybB b2995 0.75 -0.9
3,141,901 - hybB b2995 0.76 -0.5
3,141,901 - hybB b2995 0.76 +0.4
3,141,913 - hybB b2995 0.77 -0.1
3,141,955 - hybB b2995 0.80 -0.4
3,141,955 - hybB b2995 0.80 -1.0

Or see this region's nucleotide sequence