Strain Fitness in Escherichia coli BW25113 around b2827

Experiment: LB Anaerobic with Sulfamonomethoxine 0.0000625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntppdB and ppdA overlap by 10 nucleotidesppdA and thyA are separated by 183 nucleotidesthyA and lgt are separated by 6 nucleotides b2825: ppdB - hypothetical protein (NCBI), at 2,961,175 to 2,961,738 ppdB b2826: ppdA - hypothetical protein (NCBI), at 2,961,729 to 2,962,199 ppdA b2827: thyA - thymidylate synthase (NCBI), at 2,962,383 to 2,963,177 thyA b2828: lgt - prolipoprotein diacylglyceryl transferase (NCBI), at 2,963,184 to 2,964,059 lgt Position (kb) 2962 2963 2964Strain fitness (log2 ratio) -2 -1 0 1at 2961.469 kb on + strand, within ppdBat 2961.469 kb on + strand, within ppdBat 2961.483 kb on - strand, within ppdBat 2961.483 kb on - strand, within ppdBat 2961.493 kb on - strand, within ppdBat 2961.493 kb on - strand, within ppdBat 2961.518 kb on + strand, within ppdBat 2961.529 kb on - strand, within ppdBat 2961.529 kb on - strand, within ppdBat 2961.543 kb on - strand, within ppdBat 2961.543 kb on - strand, within ppdBat 2961.543 kb on - strand, within ppdBat 2961.664 kb on + strand, within ppdBat 2961.664 kb on + strand, within ppdBat 2961.794 kb on + strand, within ppdAat 2961.794 kb on + strand, within ppdAat 2961.832 kb on - strand, within ppdAat 2961.832 kb on - strand, within ppdAat 2961.852 kb on - strand, within ppdAat 2961.875 kb on + strand, within ppdAat 2961.875 kb on + strand, within ppdAat 2961.906 kb on + strand, within ppdAat 2961.918 kb on + strand, within ppdAat 2961.943 kb on + strand, within ppdAat 2961.943 kb on + strand, within ppdAat 2961.958 kb on + strand, within ppdAat 2961.958 kb on + strand, within ppdAat 2961.963 kb on + strand, within ppdAat 2962.113 kb on + strand, within ppdAat 2962.122 kb on - strand, within ppdAat 2962.130 kb on + strand, within ppdAat 2962.130 kb on + strand, within ppdAat 2962.149 kb on - strand, within ppdAat 2962.149 kb on - strand, within ppdAat 2962.151 kb on + strand, within ppdAat 2962.173 kb on - strandat 2962.175 kb on + strandat 2962.175 kb on + strandat 2962.175 kb on + strandat 2962.175 kb on + strandat 2962.183 kb on - strandat 2962.215 kb on - strandat 2962.271 kb on + strandat 2962.281 kb on + strandat 2962.281 kb on + strandat 2962.289 kb on - strandat 2962.289 kb on - strandat 2963.201 kb on - strandat 2963.239 kb on - strandat 2964.140 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Sulfamonomethoxine 0.0000625 mM
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2,961,469 + ppdB b2825 0.52 -0.0
2,961,469 + ppdB b2825 0.52 -0.0
2,961,483 - ppdB b2825 0.55 -1.3
2,961,483 - ppdB b2825 0.55 -1.1
2,961,493 - ppdB b2825 0.56 +0.1
2,961,493 - ppdB b2825 0.56 +0.3
2,961,518 + ppdB b2825 0.61 -0.9
2,961,529 - ppdB b2825 0.63 +0.4
2,961,529 - ppdB b2825 0.63 -0.0
2,961,543 - ppdB b2825 0.65 -0.0
2,961,543 - ppdB b2825 0.65 -1.2
2,961,543 - ppdB b2825 0.65 -0.6
2,961,664 + ppdB b2825 0.87 +0.3
2,961,664 + ppdB b2825 0.87 -1.0
2,961,794 + ppdA b2826 0.14 -0.4
2,961,794 + ppdA b2826 0.14 +0.8
2,961,832 - ppdA b2826 0.22 -0.3
2,961,832 - ppdA b2826 0.22 -0.0
2,961,852 - ppdA b2826 0.26 +0.4
2,961,875 + ppdA b2826 0.31 -2.1
2,961,875 + ppdA b2826 0.31 -0.8
2,961,906 + ppdA b2826 0.38 -0.0
2,961,918 + ppdA b2826 0.40 -0.6
2,961,943 + ppdA b2826 0.45 -2.4
2,961,943 + ppdA b2826 0.45 +0.2
2,961,958 + ppdA b2826 0.49 -0.3
2,961,958 + ppdA b2826 0.49 +0.5
2,961,963 + ppdA b2826 0.50 +0.9
2,962,113 + ppdA b2826 0.82 -0.4
2,962,122 - ppdA b2826 0.83 +1.1
2,962,130 + ppdA b2826 0.85 -0.3
2,962,130 + ppdA b2826 0.85 -0.4
2,962,149 - ppdA b2826 0.89 -0.9
2,962,149 - ppdA b2826 0.89 +0.8
2,962,151 + ppdA b2826 0.90 -0.8
2,962,173 - -0.1
2,962,175 + +0.5
2,962,175 + -0.0
2,962,175 + -0.3
2,962,175 + -0.3
2,962,183 - +0.1
2,962,215 - +0.0
2,962,271 + -0.9
2,962,281 + -0.5
2,962,281 + -0.9
2,962,289 - -0.1
2,962,289 - -0.0
2,963,201 - +0.6
2,963,239 - +0.4
2,964,140 - -1.0

Or see this region's nucleotide sequence