Strain Fitness in Escherichia coli BW25113 around b3326

Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyheF and gspE are separated by 9 nucleotidesgspE and gspF overlap by 4 nucleotides b3325: yheF - putative general protein secretion protein (VIMSS), at 3,454,399 to 3,456,351 yheF b3326: gspE - general secretory pathway component, cryptic (NCBI), at 3,456,361 to 3,457,842 gspE b3327: gspF - general secretory pathway component, cryptic (NCBI), at 3,457,839 to 3,459,035 gspF Position (kb) 3456 3457 3458Strain fitness (log2 ratio) -2 -1 0 1 2at 3455.372 kb on + strand, within yheFat 3455.372 kb on + strand, within yheFat 3455.385 kb on - strand, within yheFat 3455.417 kb on - strand, within yheFat 3455.448 kb on - strand, within yheFat 3455.448 kb on - strand, within yheFat 3455.537 kb on - strand, within yheFat 3455.537 kb on - strand, within yheFat 3455.566 kb on + strand, within yheFat 3455.633 kb on - strand, within yheFat 3455.651 kb on - strand, within yheFat 3455.664 kb on + strand, within yheFat 3455.664 kb on + strand, within yheFat 3455.672 kb on - strand, within yheFat 3455.672 kb on - strand, within yheFat 3455.686 kb on + strand, within yheFat 3455.700 kb on + strand, within yheFat 3455.700 kb on + strand, within yheFat 3455.708 kb on - strand, within yheFat 3455.749 kb on - strand, within yheFat 3455.749 kb on - strand, within yheFat 3455.759 kb on - strand, within yheFat 3455.784 kb on + strand, within yheFat 3455.787 kb on - strand, within yheFat 3455.799 kb on + strand, within yheFat 3455.799 kb on + strand, within yheFat 3455.802 kb on - strand, within yheFat 3455.802 kb on - strand, within yheFat 3455.816 kb on + strand, within yheFat 3455.816 kb on + strand, within yheFat 3455.817 kb on - strand, within yheFat 3455.824 kb on - strand, within yheFat 3455.824 kb on - strand, within yheFat 3455.831 kb on - strand, within yheFat 3455.832 kb on + strand, within yheFat 3455.832 kb on + strand, within yheFat 3455.832 kb on + strand, within yheFat 3455.886 kb on + strand, within yheFat 3455.887 kb on + strand, within yheFat 3455.887 kb on + strand, within yheFat 3455.913 kb on - strand, within yheFat 3455.923 kb on - strand, within yheFat 3455.923 kb on - strand, within yheFat 3455.989 kb on + strand, within yheFat 3456.006 kb on + strand, within yheFat 3456.014 kb on - strand, within yheFat 3456.014 kb on - strand, within yheFat 3456.014 kb on - strand, within yheFat 3456.098 kb on + strand, within yheFat 3456.114 kb on + strand, within yheFat 3456.120 kb on + strand, within yheFat 3456.123 kb on + strand, within yheFat 3456.123 kb on + strand, within yheFat 3456.136 kb on - strand, within yheFat 3456.165 kb on - strandat 3456.195 kb on - strandat 3456.195 kb on - strandat 3456.233 kb on + strandat 3456.321 kb on - strandat 3456.323 kb on - strandat 3456.327 kb on + strandat 3456.327 kb on + strandat 3456.337 kb on - strandat 3456.348 kb on + strandat 3456.348 kb on - strandat 3456.376 kb on - strandat 3456.379 kb on + strandat 3456.379 kb on + strandat 3456.392 kb on - strandat 3456.498 kb on + strandat 3456.498 kb on + strandat 3456.504 kb on + strandat 3456.505 kb on + strandat 3456.506 kb on - strandat 3456.506 kb on - strandat 3456.506 kb on - strandat 3456.506 kb on - strandat 3456.506 kb on - strandat 3456.527 kb on - strand, within gspEat 3456.529 kb on + strand, within gspEat 3456.575 kb on + strand, within gspEat 3456.594 kb on + strand, within gspEat 3456.631 kb on + strand, within gspEat 3456.676 kb on + strand, within gspEat 3456.707 kb on + strand, within gspEat 3456.707 kb on + strand, within gspEat 3456.856 kb on + strand, within gspEat 3456.856 kb on + strand, within gspEat 3456.897 kb on + strand, within gspEat 3456.898 kb on - strand, within gspEat 3456.911 kb on - strand, within gspEat 3456.911 kb on - strand, within gspEat 3456.927 kb on + strand, within gspEat 3456.935 kb on - strand, within gspEat 3456.935 kb on - strand, within gspEat 3456.935 kb on - strand, within gspEat 3456.937 kb on + strand, within gspEat 3456.940 kb on - strand, within gspEat 3456.940 kb on - strand, within gspEat 3456.943 kb on + strand, within gspEat 3456.968 kb on + strand, within gspEat 3456.971 kb on - strand, within gspEat 3456.971 kb on - strand, within gspEat 3456.976 kb on - strand, within gspEat 3456.976 kb on - strand, within gspEat 3457.028 kb on + strand, within gspEat 3457.028 kb on + strand, within gspEat 3457.031 kb on - strand, within gspEat 3457.043 kb on - strand, within gspEat 3457.054 kb on - strand, within gspEat 3457.088 kb on + strand, within gspEat 3457.088 kb on + strand, within gspEat 3457.088 kb on + strand, within gspEat 3457.088 kb on + strand, within gspEat 3457.088 kb on + strand, within gspEat 3457.088 kb on + strand, within gspEat 3457.093 kb on + strand, within gspEat 3457.096 kb on - strand, within gspEat 3457.096 kb on - strand, within gspEat 3457.106 kb on - strand, within gspEat 3457.134 kb on + strand, within gspEat 3457.143 kb on - strand, within gspEat 3457.186 kb on + strand, within gspEat 3457.234 kb on + strand, within gspEat 3457.234 kb on + strand, within gspEat 3457.241 kb on - strand, within gspEat 3457.242 kb on - strand, within gspEat 3457.252 kb on - strand, within gspEat 3457.254 kb on - strand, within gspEat 3457.254 kb on - strand, within gspEat 3457.262 kb on + strand, within gspEat 3457.262 kb on + strand, within gspEat 3457.274 kb on + strand, within gspEat 3457.275 kb on + strand, within gspEat 3457.301 kb on - strand, within gspEat 3457.378 kb on - strand, within gspEat 3457.387 kb on - strand, within gspEat 3457.387 kb on - strand, within gspEat 3457.387 kb on - strand, within gspEat 3457.400 kb on - strand, within gspEat 3457.400 kb on - strand, within gspEat 3457.404 kb on + strand, within gspEat 3457.422 kb on + strand, within gspEat 3457.455 kb on + strand, within gspEat 3457.504 kb on + strand, within gspEat 3457.541 kb on + strand, within gspEat 3457.594 kb on + strand, within gspEat 3457.594 kb on + strand, within gspEat 3457.594 kb on + strand, within gspEat 3457.602 kb on - strand, within gspEat 3457.604 kb on + strand, within gspEat 3457.752 kb on - strandat 3457.779 kb on - strandat 3457.788 kb on + strandat 3457.788 kb on + strandat 3457.788 kb on + strandat 3457.830 kb on - strandat 3457.830 kb on - strandat 3457.833 kb on - strandat 3457.862 kb on + strandat 3457.863 kb on + strandat 3457.864 kb on - strandat 3457.864 kb on - strandat 3457.870 kb on - strandat 3457.871 kb on - strandat 3457.871 kb on - strandat 3457.889 kb on + strandat 3457.963 kb on - strand, within gspFat 3458.010 kb on - strand, within gspFat 3458.011 kb on + strand, within gspFat 3458.019 kb on - strand, within gspFat 3458.048 kb on + strand, within gspFat 3458.067 kb on + strand, within gspFat 3458.067 kb on + strand, within gspFat 3458.074 kb on - strand, within gspFat 3458.085 kb on + strand, within gspFat 3458.093 kb on - strand, within gspFat 3458.113 kb on - strand, within gspFat 3458.141 kb on + strand, within gspFat 3458.218 kb on + strand, within gspFat 3458.223 kb on - strand, within gspFat 3458.223 kb on - strand, within gspFat 3458.223 kb on - strand, within gspFat 3458.223 kb on - strand, within gspFat 3458.223 kb on - strand, within gspFat 3458.223 kb on - strand, within gspFat 3458.243 kb on - strand, within gspFat 3458.243 kb on - strand, within gspFat 3458.324 kb on + strand, within gspFat 3458.324 kb on + strand, within gspFat 3458.324 kb on + strand, within gspFat 3458.338 kb on - strand, within gspFat 3458.338 kb on - strand, within gspFat 3458.348 kb on + strand, within gspFat 3458.348 kb on + strand, within gspFat 3458.353 kb on - strand, within gspFat 3458.358 kb on - strand, within gspFat 3458.406 kb on + strand, within gspFat 3458.420 kb on + strand, within gspFat 3458.420 kb on + strand, within gspFat 3458.428 kb on - strand, within gspFat 3458.453 kb on + strand, within gspFat 3458.475 kb on + strand, within gspFat 3458.505 kb on - strand, within gspFat 3458.521 kb on - strand, within gspFat 3458.521 kb on - strand, within gspFat 3458.632 kb on - strand, within gspFat 3458.632 kb on - strand, within gspFat 3458.638 kb on + strand, within gspFat 3458.663 kb on - strand, within gspFat 3458.668 kb on + strand, within gspFat 3458.671 kb on - strand, within gspFat 3458.687 kb on - strand, within gspFat 3458.687 kb on - strand, within gspFat 3458.689 kb on + strand, within gspFat 3458.711 kb on + strand, within gspFat 3458.817 kb on + strand, within gspFat 3458.817 kb on + strand, within gspFat 3458.822 kb on + strand, within gspFat 3458.822 kb on + strand, within gspFat 3458.823 kb on + strand, within gspF

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.00000195313 mM
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3,455,372 + yheF b3325 0.50 -0.6
3,455,372 + yheF b3325 0.50 -0.1
3,455,385 - yheF b3325 0.50 +0.3
3,455,417 - yheF b3325 0.52 -1.4
3,455,448 - yheF b3325 0.54 +0.9
3,455,448 - yheF b3325 0.54 -0.3
3,455,537 - yheF b3325 0.58 -1.5
3,455,537 - yheF b3325 0.58 +0.7
3,455,566 + yheF b3325 0.60 +0.2
3,455,633 - yheF b3325 0.63 -1.2
3,455,651 - yheF b3325 0.64 -0.5
3,455,664 + yheF b3325 0.65 +0.0
3,455,664 + yheF b3325 0.65 +0.2
3,455,672 - yheF b3325 0.65 -0.0
3,455,672 - yheF b3325 0.65 -0.7
3,455,686 + yheF b3325 0.66 -0.5
3,455,700 + yheF b3325 0.67 +0.7
3,455,700 + yheF b3325 0.67 +0.1
3,455,708 - yheF b3325 0.67 +0.6
3,455,749 - yheF b3325 0.69 +0.3
3,455,749 - yheF b3325 0.69 -0.7
3,455,759 - yheF b3325 0.70 -0.3
3,455,784 + yheF b3325 0.71 -0.5
3,455,787 - yheF b3325 0.71 +0.6
3,455,799 + yheF b3325 0.72 +0.7
3,455,799 + yheF b3325 0.72 +0.2
3,455,802 - yheF b3325 0.72 -0.0
3,455,802 - yheF b3325 0.72 +0.2
3,455,816 + yheF b3325 0.73 -0.7
3,455,816 + yheF b3325 0.73 +0.1
3,455,817 - yheF b3325 0.73 +0.1
3,455,824 - yheF b3325 0.73 +0.6
3,455,824 - yheF b3325 0.73 +0.3
3,455,831 - yheF b3325 0.73 +0.3
3,455,832 + yheF b3325 0.73 +0.7
3,455,832 + yheF b3325 0.73 +0.1
3,455,832 + yheF b3325 0.73 +0.0
3,455,886 + yheF b3325 0.76 +0.9
3,455,887 + yheF b3325 0.76 +0.3
3,455,887 + yheF b3325 0.76 +0.9
3,455,913 - yheF b3325 0.78 -0.1
3,455,923 - yheF b3325 0.78 -0.2
3,455,923 - yheF b3325 0.78 -0.6
3,455,989 + yheF b3325 0.81 -0.7
3,456,006 + yheF b3325 0.82 -0.6
3,456,014 - yheF b3325 0.83 -0.4
3,456,014 - yheF b3325 0.83 -0.8
3,456,014 - yheF b3325 0.83 +1.1
3,456,098 + yheF b3325 0.87 -0.3
3,456,114 + yheF b3325 0.88 +0.3
3,456,120 + yheF b3325 0.88 +0.5
3,456,123 + yheF b3325 0.88 +0.1
3,456,123 + yheF b3325 0.88 +0.4
3,456,136 - yheF b3325 0.89 +0.3
3,456,165 - +0.6
3,456,195 - +0.6
3,456,195 - +0.5
3,456,233 + +0.1
3,456,321 - -0.2
3,456,323 - +0.0
3,456,327 + -1.0
3,456,327 + +0.7
3,456,337 - -0.2
3,456,348 + +0.3
3,456,348 - -0.7
3,456,376 - -0.2
3,456,379 + +0.3
3,456,379 + +0.9
3,456,392 - -0.2
3,456,498 + -0.1
3,456,498 + -2.2
3,456,504 + -0.8
3,456,505 + -0.2
3,456,506 - -1.4
3,456,506 - +0.3
3,456,506 - -0.4
3,456,506 - +0.4
3,456,506 - +1.0
3,456,527 - gspE b3326 0.11 +0.1
3,456,529 + gspE b3326 0.11 +0.6
3,456,575 + gspE b3326 0.14 -0.1
3,456,594 + gspE b3326 0.16 +0.1
3,456,631 + gspE b3326 0.18 +0.1
3,456,676 + gspE b3326 0.21 +0.3
3,456,707 + gspE b3326 0.23 +0.2
3,456,707 + gspE b3326 0.23 +1.0
3,456,856 + gspE b3326 0.33 -0.3
3,456,856 + gspE b3326 0.33 +0.3
3,456,897 + gspE b3326 0.36 +0.0
3,456,898 - gspE b3326 0.36 +0.0
3,456,911 - gspE b3326 0.37 +1.3
3,456,911 - gspE b3326 0.37 +0.7
3,456,927 + gspE b3326 0.38 +0.5
3,456,935 - gspE b3326 0.39 -0.2
3,456,935 - gspE b3326 0.39 +1.2
3,456,935 - gspE b3326 0.39 +0.2
3,456,937 + gspE b3326 0.39 +0.4
3,456,940 - gspE b3326 0.39 -0.6
3,456,940 - gspE b3326 0.39 -0.3
3,456,943 + gspE b3326 0.39 -0.0
3,456,968 + gspE b3326 0.41 +0.4
3,456,971 - gspE b3326 0.41 -0.9
3,456,971 - gspE b3326 0.41 -0.1
3,456,976 - gspE b3326 0.41 -1.4
3,456,976 - gspE b3326 0.41 +0.9
3,457,028 + gspE b3326 0.45 +0.1
3,457,028 + gspE b3326 0.45 +0.8
3,457,031 - gspE b3326 0.45 +0.8
3,457,043 - gspE b3326 0.46 +0.3
3,457,054 - gspE b3326 0.47 +1.4
3,457,088 + gspE b3326 0.49 +0.8
3,457,088 + gspE b3326 0.49 +0.1
3,457,088 + gspE b3326 0.49 +1.6
3,457,088 + gspE b3326 0.49 +0.1
3,457,088 + gspE b3326 0.49 +0.1
3,457,088 + gspE b3326 0.49 +0.0
3,457,093 + gspE b3326 0.49 +0.7
3,457,096 - gspE b3326 0.50 +0.6
3,457,096 - gspE b3326 0.50 -0.1
3,457,106 - gspE b3326 0.50 -0.0
3,457,134 + gspE b3326 0.52 -0.2
3,457,143 - gspE b3326 0.53 -1.1
3,457,186 + gspE b3326 0.56 -0.2
3,457,234 + gspE b3326 0.59 +0.2
3,457,234 + gspE b3326 0.59 +1.4
3,457,241 - gspE b3326 0.59 -0.4
3,457,242 - gspE b3326 0.59 +0.7
3,457,252 - gspE b3326 0.60 +0.9
3,457,254 - gspE b3326 0.60 -1.0
3,457,254 - gspE b3326 0.60 +0.2
3,457,262 + gspE b3326 0.61 +1.5
3,457,262 + gspE b3326 0.61 -0.3
3,457,274 + gspE b3326 0.62 -0.1
3,457,275 + gspE b3326 0.62 -0.3
3,457,301 - gspE b3326 0.63 +0.2
3,457,378 - gspE b3326 0.69 -0.7
3,457,387 - gspE b3326 0.69 -2.4
3,457,387 - gspE b3326 0.69 +0.6
3,457,387 - gspE b3326 0.69 -0.5
3,457,400 - gspE b3326 0.70 -0.7
3,457,400 - gspE b3326 0.70 -0.2
3,457,404 + gspE b3326 0.70 -0.6
3,457,422 + gspE b3326 0.72 -0.2
3,457,455 + gspE b3326 0.74 +0.6
3,457,504 + gspE b3326 0.77 +0.6
3,457,541 + gspE b3326 0.80 -0.1
3,457,594 + gspE b3326 0.83 -0.4
3,457,594 + gspE b3326 0.83 -0.1
3,457,594 + gspE b3326 0.83 +0.6
3,457,602 - gspE b3326 0.84 +0.4
3,457,604 + gspE b3326 0.84 -0.1
3,457,752 - +0.3
3,457,779 - -0.5
3,457,788 + -0.8
3,457,788 + -0.0
3,457,788 + +0.1
3,457,830 - -0.9
3,457,830 - +0.9
3,457,833 - +0.3
3,457,862 + +0.2
3,457,863 + +0.1
3,457,864 - +0.2
3,457,864 - -0.7
3,457,870 - +0.7
3,457,871 - -0.0
3,457,871 - +0.3
3,457,889 + -0.5
3,457,963 - gspF b3327 0.10 -0.2
3,458,010 - gspF b3327 0.14 -0.6
3,458,011 + gspF b3327 0.14 +0.4
3,458,019 - gspF b3327 0.15 +0.9
3,458,048 + gspF b3327 0.17 -1.1
3,458,067 + gspF b3327 0.19 -0.4
3,458,067 + gspF b3327 0.19 -1.5
3,458,074 - gspF b3327 0.20 +0.5
3,458,085 + gspF b3327 0.21 -0.2
3,458,093 - gspF b3327 0.21 -0.2
3,458,113 - gspF b3327 0.23 -1.1
3,458,141 + gspF b3327 0.25 -0.2
3,458,218 + gspF b3327 0.32 -0.6
3,458,223 - gspF b3327 0.32 +0.2
3,458,223 - gspF b3327 0.32 +0.2
3,458,223 - gspF b3327 0.32 +0.9
3,458,223 - gspF b3327 0.32 -0.5
3,458,223 - gspF b3327 0.32 -0.6
3,458,223 - gspF b3327 0.32 +0.6
3,458,243 - gspF b3327 0.34 -0.1
3,458,243 - gspF b3327 0.34 -1.8
3,458,324 + gspF b3327 0.41 -0.6
3,458,324 + gspF b3327 0.41 -2.7
3,458,324 + gspF b3327 0.41 +1.0
3,458,338 - gspF b3327 0.42 -1.0
3,458,338 - gspF b3327 0.42 +0.4
3,458,348 + gspF b3327 0.43 +0.1
3,458,348 + gspF b3327 0.43 +0.5
3,458,353 - gspF b3327 0.43 -1.0
3,458,358 - gspF b3327 0.43 -0.2
3,458,406 + gspF b3327 0.47 -0.1
3,458,420 + gspF b3327 0.49 -0.2
3,458,420 + gspF b3327 0.49 -0.3
3,458,428 - gspF b3327 0.49 +0.8
3,458,453 + gspF b3327 0.51 -0.3
3,458,475 + gspF b3327 0.53 +0.1
3,458,505 - gspF b3327 0.56 -0.1
3,458,521 - gspF b3327 0.57 -0.5
3,458,521 - gspF b3327 0.57 +1.9
3,458,632 - gspF b3327 0.66 +0.3
3,458,632 - gspF b3327 0.66 -0.2
3,458,638 + gspF b3327 0.67 +0.4
3,458,663 - gspF b3327 0.69 +0.3
3,458,668 + gspF b3327 0.69 +0.6
3,458,671 - gspF b3327 0.70 -0.4
3,458,687 - gspF b3327 0.71 +0.6
3,458,687 - gspF b3327 0.71 +1.1
3,458,689 + gspF b3327 0.71 -1.1
3,458,711 + gspF b3327 0.73 +0.5
3,458,817 + gspF b3327 0.82 +0.2
3,458,817 + gspF b3327 0.82 -0.2
3,458,822 + gspF b3327 0.82 +1.3
3,458,822 + gspF b3327 0.82 +2.1
3,458,823 + gspF b3327 0.82 -0.7

Or see this region's nucleotide sequence