Strain Fitness in Escherichia coli BW25113 around b3326
Experiment: LB Anaerobic with Nalidixic 0.00000195313 mM
Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.
Per-strain Table
Position | Strand | Gene | LocusTag | Fraction | LB Anaerobic with Nalidixic 0.00000195313 mM |
---|---|---|---|---|---|
remove | |||||
3,455,372 | + | yheF | b3325 | 0.50 | -0.6 |
3,455,372 | + | yheF | b3325 | 0.50 | -0.1 |
3,455,385 | - | yheF | b3325 | 0.50 | +0.3 |
3,455,417 | - | yheF | b3325 | 0.52 | -1.4 |
3,455,448 | - | yheF | b3325 | 0.54 | +0.9 |
3,455,448 | - | yheF | b3325 | 0.54 | -0.3 |
3,455,537 | - | yheF | b3325 | 0.58 | -1.5 |
3,455,537 | - | yheF | b3325 | 0.58 | +0.7 |
3,455,566 | + | yheF | b3325 | 0.60 | +0.2 |
3,455,633 | - | yheF | b3325 | 0.63 | -1.2 |
3,455,651 | - | yheF | b3325 | 0.64 | -0.5 |
3,455,664 | + | yheF | b3325 | 0.65 | +0.0 |
3,455,664 | + | yheF | b3325 | 0.65 | +0.2 |
3,455,672 | - | yheF | b3325 | 0.65 | -0.0 |
3,455,672 | - | yheF | b3325 | 0.65 | -0.7 |
3,455,686 | + | yheF | b3325 | 0.66 | -0.5 |
3,455,700 | + | yheF | b3325 | 0.67 | +0.7 |
3,455,700 | + | yheF | b3325 | 0.67 | +0.1 |
3,455,708 | - | yheF | b3325 | 0.67 | +0.6 |
3,455,749 | - | yheF | b3325 | 0.69 | +0.3 |
3,455,749 | - | yheF | b3325 | 0.69 | -0.7 |
3,455,759 | - | yheF | b3325 | 0.70 | -0.3 |
3,455,784 | + | yheF | b3325 | 0.71 | -0.5 |
3,455,787 | - | yheF | b3325 | 0.71 | +0.6 |
3,455,799 | + | yheF | b3325 | 0.72 | +0.7 |
3,455,799 | + | yheF | b3325 | 0.72 | +0.2 |
3,455,802 | - | yheF | b3325 | 0.72 | -0.0 |
3,455,802 | - | yheF | b3325 | 0.72 | +0.2 |
3,455,816 | + | yheF | b3325 | 0.73 | -0.7 |
3,455,816 | + | yheF | b3325 | 0.73 | +0.1 |
3,455,817 | - | yheF | b3325 | 0.73 | +0.1 |
3,455,824 | - | yheF | b3325 | 0.73 | +0.6 |
3,455,824 | - | yheF | b3325 | 0.73 | +0.3 |
3,455,831 | - | yheF | b3325 | 0.73 | +0.3 |
3,455,832 | + | yheF | b3325 | 0.73 | +0.7 |
3,455,832 | + | yheF | b3325 | 0.73 | +0.1 |
3,455,832 | + | yheF | b3325 | 0.73 | +0.0 |
3,455,886 | + | yheF | b3325 | 0.76 | +0.9 |
3,455,887 | + | yheF | b3325 | 0.76 | +0.3 |
3,455,887 | + | yheF | b3325 | 0.76 | +0.9 |
3,455,913 | - | yheF | b3325 | 0.78 | -0.1 |
3,455,923 | - | yheF | b3325 | 0.78 | -0.2 |
3,455,923 | - | yheF | b3325 | 0.78 | -0.6 |
3,455,989 | + | yheF | b3325 | 0.81 | -0.7 |
3,456,006 | + | yheF | b3325 | 0.82 | -0.6 |
3,456,014 | - | yheF | b3325 | 0.83 | -0.4 |
3,456,014 | - | yheF | b3325 | 0.83 | -0.8 |
3,456,014 | - | yheF | b3325 | 0.83 | +1.1 |
3,456,098 | + | yheF | b3325 | 0.87 | -0.3 |
3,456,114 | + | yheF | b3325 | 0.88 | +0.3 |
3,456,120 | + | yheF | b3325 | 0.88 | +0.5 |
3,456,123 | + | yheF | b3325 | 0.88 | +0.1 |
3,456,123 | + | yheF | b3325 | 0.88 | +0.4 |
3,456,136 | - | yheF | b3325 | 0.89 | +0.3 |
3,456,165 | - | +0.6 | |||
3,456,195 | - | +0.6 | |||
3,456,195 | - | +0.5 | |||
3,456,233 | + | +0.1 | |||
3,456,321 | - | -0.2 | |||
3,456,323 | - | +0.0 | |||
3,456,327 | + | -1.0 | |||
3,456,327 | + | +0.7 | |||
3,456,337 | - | -0.2 | |||
3,456,348 | + | +0.3 | |||
3,456,348 | - | -0.7 | |||
3,456,376 | - | -0.2 | |||
3,456,379 | + | +0.3 | |||
3,456,379 | + | +0.9 | |||
3,456,392 | - | -0.2 | |||
3,456,498 | + | -0.1 | |||
3,456,498 | + | -2.2 | |||
3,456,504 | + | -0.8 | |||
3,456,505 | + | -0.2 | |||
3,456,506 | - | -1.4 | |||
3,456,506 | - | +0.3 | |||
3,456,506 | - | -0.4 | |||
3,456,506 | - | +0.4 | |||
3,456,506 | - | +1.0 | |||
3,456,527 | - | gspE | b3326 | 0.11 | +0.1 |
3,456,529 | + | gspE | b3326 | 0.11 | +0.6 |
3,456,575 | + | gspE | b3326 | 0.14 | -0.1 |
3,456,594 | + | gspE | b3326 | 0.16 | +0.1 |
3,456,631 | + | gspE | b3326 | 0.18 | +0.1 |
3,456,676 | + | gspE | b3326 | 0.21 | +0.3 |
3,456,707 | + | gspE | b3326 | 0.23 | +0.2 |
3,456,707 | + | gspE | b3326 | 0.23 | +1.0 |
3,456,856 | + | gspE | b3326 | 0.33 | -0.3 |
3,456,856 | + | gspE | b3326 | 0.33 | +0.3 |
3,456,897 | + | gspE | b3326 | 0.36 | +0.0 |
3,456,898 | - | gspE | b3326 | 0.36 | +0.0 |
3,456,911 | - | gspE | b3326 | 0.37 | +1.3 |
3,456,911 | - | gspE | b3326 | 0.37 | +0.7 |
3,456,927 | + | gspE | b3326 | 0.38 | +0.5 |
3,456,935 | - | gspE | b3326 | 0.39 | -0.2 |
3,456,935 | - | gspE | b3326 | 0.39 | +1.2 |
3,456,935 | - | gspE | b3326 | 0.39 | +0.2 |
3,456,937 | + | gspE | b3326 | 0.39 | +0.4 |
3,456,940 | - | gspE | b3326 | 0.39 | -0.6 |
3,456,940 | - | gspE | b3326 | 0.39 | -0.3 |
3,456,943 | + | gspE | b3326 | 0.39 | -0.0 |
3,456,968 | + | gspE | b3326 | 0.41 | +0.4 |
3,456,971 | - | gspE | b3326 | 0.41 | -0.9 |
3,456,971 | - | gspE | b3326 | 0.41 | -0.1 |
3,456,976 | - | gspE | b3326 | 0.41 | -1.4 |
3,456,976 | - | gspE | b3326 | 0.41 | +0.9 |
3,457,028 | + | gspE | b3326 | 0.45 | +0.1 |
3,457,028 | + | gspE | b3326 | 0.45 | +0.8 |
3,457,031 | - | gspE | b3326 | 0.45 | +0.8 |
3,457,043 | - | gspE | b3326 | 0.46 | +0.3 |
3,457,054 | - | gspE | b3326 | 0.47 | +1.4 |
3,457,088 | + | gspE | b3326 | 0.49 | +0.8 |
3,457,088 | + | gspE | b3326 | 0.49 | +0.1 |
3,457,088 | + | gspE | b3326 | 0.49 | +1.6 |
3,457,088 | + | gspE | b3326 | 0.49 | +0.1 |
3,457,088 | + | gspE | b3326 | 0.49 | +0.1 |
3,457,088 | + | gspE | b3326 | 0.49 | +0.0 |
3,457,093 | + | gspE | b3326 | 0.49 | +0.7 |
3,457,096 | - | gspE | b3326 | 0.50 | +0.6 |
3,457,096 | - | gspE | b3326 | 0.50 | -0.1 |
3,457,106 | - | gspE | b3326 | 0.50 | -0.0 |
3,457,134 | + | gspE | b3326 | 0.52 | -0.2 |
3,457,143 | - | gspE | b3326 | 0.53 | -1.1 |
3,457,186 | + | gspE | b3326 | 0.56 | -0.2 |
3,457,234 | + | gspE | b3326 | 0.59 | +0.2 |
3,457,234 | + | gspE | b3326 | 0.59 | +1.4 |
3,457,241 | - | gspE | b3326 | 0.59 | -0.4 |
3,457,242 | - | gspE | b3326 | 0.59 | +0.7 |
3,457,252 | - | gspE | b3326 | 0.60 | +0.9 |
3,457,254 | - | gspE | b3326 | 0.60 | -1.0 |
3,457,254 | - | gspE | b3326 | 0.60 | +0.2 |
3,457,262 | + | gspE | b3326 | 0.61 | +1.5 |
3,457,262 | + | gspE | b3326 | 0.61 | -0.3 |
3,457,274 | + | gspE | b3326 | 0.62 | -0.1 |
3,457,275 | + | gspE | b3326 | 0.62 | -0.3 |
3,457,301 | - | gspE | b3326 | 0.63 | +0.2 |
3,457,378 | - | gspE | b3326 | 0.69 | -0.7 |
3,457,387 | - | gspE | b3326 | 0.69 | -2.4 |
3,457,387 | - | gspE | b3326 | 0.69 | +0.6 |
3,457,387 | - | gspE | b3326 | 0.69 | -0.5 |
3,457,400 | - | gspE | b3326 | 0.70 | -0.7 |
3,457,400 | - | gspE | b3326 | 0.70 | -0.2 |
3,457,404 | + | gspE | b3326 | 0.70 | -0.6 |
3,457,422 | + | gspE | b3326 | 0.72 | -0.2 |
3,457,455 | + | gspE | b3326 | 0.74 | +0.6 |
3,457,504 | + | gspE | b3326 | 0.77 | +0.6 |
3,457,541 | + | gspE | b3326 | 0.80 | -0.1 |
3,457,594 | + | gspE | b3326 | 0.83 | -0.4 |
3,457,594 | + | gspE | b3326 | 0.83 | -0.1 |
3,457,594 | + | gspE | b3326 | 0.83 | +0.6 |
3,457,602 | - | gspE | b3326 | 0.84 | +0.4 |
3,457,604 | + | gspE | b3326 | 0.84 | -0.1 |
3,457,752 | - | +0.3 | |||
3,457,779 | - | -0.5 | |||
3,457,788 | + | -0.8 | |||
3,457,788 | + | -0.0 | |||
3,457,788 | + | +0.1 | |||
3,457,830 | - | -0.9 | |||
3,457,830 | - | +0.9 | |||
3,457,833 | - | +0.3 | |||
3,457,862 | + | +0.2 | |||
3,457,863 | + | +0.1 | |||
3,457,864 | - | +0.2 | |||
3,457,864 | - | -0.7 | |||
3,457,870 | - | +0.7 | |||
3,457,871 | - | -0.0 | |||
3,457,871 | - | +0.3 | |||
3,457,889 | + | -0.5 | |||
3,457,963 | - | gspF | b3327 | 0.10 | -0.2 |
3,458,010 | - | gspF | b3327 | 0.14 | -0.6 |
3,458,011 | + | gspF | b3327 | 0.14 | +0.4 |
3,458,019 | - | gspF | b3327 | 0.15 | +0.9 |
3,458,048 | + | gspF | b3327 | 0.17 | -1.1 |
3,458,067 | + | gspF | b3327 | 0.19 | -0.4 |
3,458,067 | + | gspF | b3327 | 0.19 | -1.5 |
3,458,074 | - | gspF | b3327 | 0.20 | +0.5 |
3,458,085 | + | gspF | b3327 | 0.21 | -0.2 |
3,458,093 | - | gspF | b3327 | 0.21 | -0.2 |
3,458,113 | - | gspF | b3327 | 0.23 | -1.1 |
3,458,141 | + | gspF | b3327 | 0.25 | -0.2 |
3,458,218 | + | gspF | b3327 | 0.32 | -0.6 |
3,458,223 | - | gspF | b3327 | 0.32 | +0.2 |
3,458,223 | - | gspF | b3327 | 0.32 | +0.2 |
3,458,223 | - | gspF | b3327 | 0.32 | +0.9 |
3,458,223 | - | gspF | b3327 | 0.32 | -0.5 |
3,458,223 | - | gspF | b3327 | 0.32 | -0.6 |
3,458,223 | - | gspF | b3327 | 0.32 | +0.6 |
3,458,243 | - | gspF | b3327 | 0.34 | -0.1 |
3,458,243 | - | gspF | b3327 | 0.34 | -1.8 |
3,458,324 | + | gspF | b3327 | 0.41 | -0.6 |
3,458,324 | + | gspF | b3327 | 0.41 | -2.7 |
3,458,324 | + | gspF | b3327 | 0.41 | +1.0 |
3,458,338 | - | gspF | b3327 | 0.42 | -1.0 |
3,458,338 | - | gspF | b3327 | 0.42 | +0.4 |
3,458,348 | + | gspF | b3327 | 0.43 | +0.1 |
3,458,348 | + | gspF | b3327 | 0.43 | +0.5 |
3,458,353 | - | gspF | b3327 | 0.43 | -1.0 |
3,458,358 | - | gspF | b3327 | 0.43 | -0.2 |
3,458,406 | + | gspF | b3327 | 0.47 | -0.1 |
3,458,420 | + | gspF | b3327 | 0.49 | -0.2 |
3,458,420 | + | gspF | b3327 | 0.49 | -0.3 |
3,458,428 | - | gspF | b3327 | 0.49 | +0.8 |
3,458,453 | + | gspF | b3327 | 0.51 | -0.3 |
3,458,475 | + | gspF | b3327 | 0.53 | +0.1 |
3,458,505 | - | gspF | b3327 | 0.56 | -0.1 |
3,458,521 | - | gspF | b3327 | 0.57 | -0.5 |
3,458,521 | - | gspF | b3327 | 0.57 | +1.9 |
3,458,632 | - | gspF | b3327 | 0.66 | +0.3 |
3,458,632 | - | gspF | b3327 | 0.66 | -0.2 |
3,458,638 | + | gspF | b3327 | 0.67 | +0.4 |
3,458,663 | - | gspF | b3327 | 0.69 | +0.3 |
3,458,668 | + | gspF | b3327 | 0.69 | +0.6 |
3,458,671 | - | gspF | b3327 | 0.70 | -0.4 |
3,458,687 | - | gspF | b3327 | 0.71 | +0.6 |
3,458,687 | - | gspF | b3327 | 0.71 | +1.1 |
3,458,689 | + | gspF | b3327 | 0.71 | -1.1 |
3,458,711 | + | gspF | b3327 | 0.73 | +0.5 |
3,458,817 | + | gspF | b3327 | 0.82 | +0.2 |
3,458,817 | + | gspF | b3327 | 0.82 | -0.2 |
3,458,822 | + | gspF | b3327 | 0.82 | +1.3 |
3,458,822 | + | gspF | b3327 | 0.82 | +2.1 |
3,458,823 | + | gspF | b3327 | 0.82 | -0.7 |
Or see this region's nucleotide sequence