Experiment: LB Anaerobic with Nalidixic 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysB and yciX_1 are separated by 330 nucleotides yciX_1 and yciX are separated by 2 nucleotides yciX and acnA are separated by 372 nucleotides
b1275: cysB - DNA-binding transcriptional dual regulator, O-acetyl-L-serine-binding (NCBI), at 1,331,879 to 1,332,853
cysB
b4522: yciX_1 - hypothetical protein (NCBI), at 1,333,184 to 1,333,312
_1
b4523: yciX - hypothetical protein (RefSeq), at 1,333,315 to 1,333,482
yciX
b1276: acnA - aconitate hydratase (NCBI), at 1,333,855 to 1,336,530
acnA
Position (kb)
1333
1334 Strain fitness (log2 ratio)
-1
0
1 at 1332.440 kb on - strand at 1332.922 kb on + strand at 1332.930 kb on - strand at 1332.930 kb on - strand at 1333.156 kb on + strand at 1333.158 kb on - strand at 1333.160 kb on - strand at 1333.197 kb on + strand, within yciX_1 at 1333.198 kb on + strand, within yciX_1 at 1333.275 kb on - strand, within yciX_1 at 1333.286 kb on - strand, within yciX_1 at 1333.286 kb on - strand, within yciX_1 at 1333.301 kb on + strand at 1333.301 kb on + strand at 1333.311 kb on + strand at 1333.320 kb on - strand at 1333.320 kb on - strand at 1333.322 kb on + strand at 1333.322 kb on + strand at 1333.345 kb on + strand, within yciX at 1333.345 kb on + strand, within yciX at 1333.345 kb on + strand, within yciX at 1333.349 kb on + strand, within yciX at 1333.418 kb on + strand, within yciX at 1333.420 kb on - strand, within yciX at 1333.434 kb on - strand, within yciX at 1333.450 kb on - strand, within yciX at 1333.450 kb on - strand, within yciX at 1333.525 kb on + strand at 1333.538 kb on + strand at 1333.578 kb on + strand at 1333.606 kb on - strand at 1333.606 kb on - strand at 1333.606 kb on - strand at 1333.606 kb on - strand at 1333.630 kb on - strand at 1333.655 kb on + strand at 1333.670 kb on + strand at 1333.670 kb on + strand at 1333.678 kb on - strand at 1333.794 kb on + strand at 1333.794 kb on + strand at 1333.818 kb on + strand at 1333.838 kb on + strand at 1333.841 kb on - strand at 1333.847 kb on - strand at 1333.873 kb on - strand at 1333.932 kb on - strand at 1334.080 kb on - strand at 1334.080 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nalidixic 0.0000078125 mM remove 1,332,440 - +0.5 1,332,922 + +0.5 1,332,930 - +0.3 1,332,930 - -1.0 1,333,156 + -1.0 1,333,158 - +0.5 1,333,160 - +0.1 1,333,197 + yciX_1 b4522 0.10 -0.8 1,333,198 + yciX_1 b4522 0.11 -0.8 1,333,275 - yciX_1 b4522 0.71 -1.3 1,333,286 - yciX_1 b4522 0.79 +1.5 1,333,286 - yciX_1 b4522 0.79 -0.9 1,333,301 + -0.8 1,333,301 + -1.7 1,333,311 + -1.0 1,333,320 - +1.5 1,333,320 - +1.5 1,333,322 + -0.4 1,333,322 + -0.5 1,333,345 + yciX b4523 0.18 -0.1 1,333,345 + yciX b4523 0.18 -0.1 1,333,345 + yciX b4523 0.18 +1.5 1,333,349 + yciX b4523 0.20 -0.4 1,333,418 + yciX b4523 0.61 -0.3 1,333,420 - yciX b4523 0.62 +1.5 1,333,434 - yciX b4523 0.71 +1.5 1,333,450 - yciX b4523 0.80 +1.5 1,333,450 - yciX b4523 0.80 -0.1 1,333,525 + -1.4 1,333,538 + -0.5 1,333,578 + -0.9 1,333,606 - -0.3 1,333,606 - -0.5 1,333,606 - -0.9 1,333,606 - -0.4 1,333,630 - +0.7 1,333,655 + -0.7 1,333,670 + +1.1 1,333,670 + -0.9 1,333,678 - -0.5 1,333,794 + -0.8 1,333,794 + -0.8 1,333,818 + -0.1 1,333,838 + -0.2 1,333,841 - -1.5 1,333,847 - +0.1 1,333,873 - -0.6 1,333,932 - -1.3 1,334,080 - -1.6 1,334,080 - +0.6
Or see this region's nucleotide sequence